UniProtKB - P77510 (DPIB_ECOLI)
Protein
Sensor histidine kinase DpiB
Gene
dpiB
Organism
Escherichia coli (strain K12)
Status
Functioni
Member of the two-component regulatory system DpiA/DpiB, which is essential for expression of citrate-specific fermentation genes and genes involved in plasmid inheritance. Could be involved in response to both the presence of citrate and external redox conditions. Functions as a sensor kinase that phosphorylates DpiA in the presence of citrate.2 Publications
Catalytic activityi
- ATP + protein L-histidine = ADP + protein N-phospho-L-histidine. EC:2.7.13.3
Activity regulationi
Autophosphorylation is induced in vitro by dithiothreitol (DTT).1 Publication
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- histidine phosphotransfer kinase activity Source: GO_Central
- phosphorelay sensor kinase activity Source: GO_Central
- protein histidine kinase activity Source: EcoCyc
GO - Biological processi
- peptidyl-histidine phosphorylation Source: EcoCyc
- protein autophosphorylation Source: EcoCyc
- regulation of transcription, DNA-templated Source: InterPro
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Two-component regulatory system |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:G6345-MONOMER MetaCyc:G6345-MONOMER |
BRENDAi | 2.7.13.3, 2026 |
Names & Taxonomyi
Protein namesi | Recommended name: Sensor histidine kinase DpiB (EC:2.7.13.3)Alternative name(s): Sensor histidine kinase CitA |
Gene namesi | Name:dpiB Synonyms:citA, mpdB, ybeP Ordered Locus Names:b0619, JW0611 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cell inner membrane 1 Publication; Multi-pass membrane protein 1 Publication
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 21 | CytoplasmicSequence analysisAdd BLAST | 21 | |
Transmembranei | 22 – 42 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 43 – 182 | PeriplasmicSequence analysisAdd BLAST | 140 | |
Transmembranei | 183 – 203 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 204 – 552 | CytoplasmicSequence analysisAdd BLAST | 349 |
GO - Cellular componenti
- cytosol Source: EcoCyc
- integral component of plasma membrane Source: EcoCyc
- outer membrane-bounded periplasmic space Source: EcoCyc
- plasma membrane Source: EcoCyc
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 138 | G → R: Reduces strongly citrate binding. 1 Publication | 1 | |
Mutagenesisi | 478 | C → A: Loss of autophosphorylation. 1 Publication | 1 | |
Mutagenesisi | 517 | C → A: Decrease in autophosphorylation. 1 Publication | 1 | |
Mutagenesisi | 529 | C → A: Constitutively active. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000074732 | 1 – 552 | Sensor histidine kinase DpiBAdd BLAST | 552 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 347 | Phosphohistidine; by autocatalysisPROSITE-ProRule annotation | 1 |
Post-translational modificationi
Autophosphorylated.
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | P77510 |
PRIDEi | P77510 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4263372, 14 interactors |
IntActi | P77510, 6 interactors |
STRINGi | 511145.b0619 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 222 – 292 | PASAdd BLAST | 71 | |
Domaini | 344 – 541 | Histidine kinasePROSITE-ProRule annotationAdd BLAST | 198 |
Domaini
The periplasmic domain binds citrate with high affinity. The C-terminal cytoplasmic domain senses reduced conditions using a single Cys in vitro.2 Publications
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG3290, Bacteria |
HOGENOMi | CLU_020211_11_2_6 |
PhylomeDBi | P77510 |
Family and domain databases
CDDi | cd00130, PAS, 1 hit |
Gene3Di | 3.30.565.10, 1 hit |
InterProi | View protein in InterPro IPR003594, HATPase_C IPR036890, HATPase_C_sf IPR005467, His_kinase_dom IPR000014, PAS IPR035965, PAS-like_dom_sf IPR013767, PAS_fold IPR033463, sCache_3 IPR029151, Sensor-like_sf IPR004358, Sig_transdc_His_kin-like_C IPR016120, Sig_transdc_His_kin_SpoOB IPR039506, SPOB_a |
Pfami | View protein in Pfam PF02518, HATPase_c, 1 hit PF00989, PAS, 1 hit PF17203, sCache_3_2, 1 hit PF14689, SPOB_a, 1 hit |
PRINTSi | PR00344, BCTRLSENSOR |
SMARTi | View protein in SMART SM00387, HATPase_c, 1 hit SM00091, PAS, 1 hit |
SUPFAMi | SSF103190, SSF103190, 1 hit SSF55785, SSF55785, 1 hit SSF55874, SSF55874, 1 hit SSF55890, SSF55890, 1 hit |
PROSITEi | View protein in PROSITE PS50109, HIS_KIN, 1 hit |
i Sequence
Sequence statusi: Complete.
P77510-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLQLNENKQF AFFQRLAFPL RIFLLILVFS IFVIAALAQY FTASFEDYLT
60 70 80 90 100
LHVRDMAMNQ AKIIASNDSV ISAVKTRDYK RLATIANKLQ RDTDFDYVVI
110 120 130 140 150
GDRHSIRLYH PNPEKIGYPM QFTKQGALEK GESYFITGKG SMGMAMRAKT
160 170 180 190 200
PIFDDDGKVI GVVSIGYLVS KIDSWRAEFL LPMAGVFVVL LGILMLLSWF
210 220 230 240 250
LAAHIRRQMM GMEPKQIARV VRQQEALFSS VYEGLIAVDP HGYITAINRN
260 270 280 290 300
ARKMLGLSSP GRQWLGKPIV EVVRPADFFT EQIDEKRQDV VANFNGLSVI
310 320 330 340 350
ANREAIRSGD DLLGAIISFR SKDEISTLNA QLTQIKQYVE SLRTLRHEHL
360 370 380 390 400
NWMSTLNGLL QMKEYDRVLA MVQGESQAQQ QLIDSLREAF ADRQVAGLLF
410 420 430 440 450
GKVQRARELG LKMIIVPGSQ LSQLPPGLDS TEFAAIVGNL LDNAFEASLR
460 470 480 490 500
SDEGNKIVEL FLSDEGDDVV IEVADQGCGV PESLRDKIFE QGVSTRADEP
510 520 530 540 550
GEHGIGLYLI ASYVTRCGGV ITLEDNDPCG TLFSIYIPKV KPNDSSINPI
DR
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 518 – 552 | GGVIT…NPIDR → VVLSLSKIMIPAVPYFQSIF RK in AAC28951 (PubMed:9701802).CuratedAdd BLAST | 35 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U46667 Genomic DNA Translation: AAC28951.1 U82598 Genomic DNA Translation: AAB40819.1 U00096 Genomic DNA Translation: AAC73720.1 AP009048 Genomic DNA Translation: BAA35255.1 |
PIRi | A64796 |
RefSeqi | NP_415152.1, NC_000913.3 WP_000939767.1, NZ_SSZK01000032.1 |
Genome annotation databases
EnsemblBacteriai | AAC73720; AAC73720; b0619 BAA35255; BAA35255; BAA35255 |
GeneIDi | 945233 |
KEGGi | ecj:JW0611 eco:b0619 |
PATRICi | fig|511145.12.peg.649 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U46667 Genomic DNA Translation: AAC28951.1 U82598 Genomic DNA Translation: AAB40819.1 U00096 Genomic DNA Translation: AAC73720.1 AP009048 Genomic DNA Translation: BAA35255.1 |
PIRi | A64796 |
RefSeqi | NP_415152.1, NC_000913.3 WP_000939767.1, NZ_SSZK01000032.1 |
3D structure databases
SMRi | P77510 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4263372, 14 interactors |
IntActi | P77510, 6 interactors |
STRINGi | 511145.b0619 |
Proteomic databases
PaxDbi | P77510 |
PRIDEi | P77510 |
Genome annotation databases
EnsemblBacteriai | AAC73720; AAC73720; b0619 BAA35255; BAA35255; BAA35255 |
GeneIDi | 945233 |
KEGGi | ecj:JW0611 eco:b0619 |
PATRICi | fig|511145.12.peg.649 |
Organism-specific databases
EchoBASEi | EB3410 |
Phylogenomic databases
eggNOGi | COG3290, Bacteria |
HOGENOMi | CLU_020211_11_2_6 |
PhylomeDBi | P77510 |
Enzyme and pathway databases
BioCyci | EcoCyc:G6345-MONOMER MetaCyc:G6345-MONOMER |
BRENDAi | 2.7.13.3, 2026 |
Miscellaneous databases
PROi | PR:P77510 |
Family and domain databases
CDDi | cd00130, PAS, 1 hit |
Gene3Di | 3.30.565.10, 1 hit |
InterProi | View protein in InterPro IPR003594, HATPase_C IPR036890, HATPase_C_sf IPR005467, His_kinase_dom IPR000014, PAS IPR035965, PAS-like_dom_sf IPR013767, PAS_fold IPR033463, sCache_3 IPR029151, Sensor-like_sf IPR004358, Sig_transdc_His_kin-like_C IPR016120, Sig_transdc_His_kin_SpoOB IPR039506, SPOB_a |
Pfami | View protein in Pfam PF02518, HATPase_c, 1 hit PF00989, PAS, 1 hit PF17203, sCache_3_2, 1 hit PF14689, SPOB_a, 1 hit |
PRINTSi | PR00344, BCTRLSENSOR |
SMARTi | View protein in SMART SM00387, HATPase_c, 1 hit SM00091, PAS, 1 hit |
SUPFAMi | SSF103190, SSF103190, 1 hit SSF55785, SSF55785, 1 hit SSF55874, SSF55874, 1 hit SSF55890, SSF55890, 1 hit |
PROSITEi | View protein in PROSITE PS50109, HIS_KIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DPIB_ECOLI | |
Accessioni | P77510Primary (citable) accession number: P77510 Secondary accession number(s): O54338 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | February 1, 1997 | |
Last modified: | December 2, 2020 | |
This is version 170 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |