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Entry version 125 (02 Dec 2020)
Sequence version 1 (01 Feb 1997)
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Protein

D-glucoside 3-dehydrogenase

Gene

ycjS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the NADH-dependent reduction of the oxo group at C3 of 3-dehydro-D-glucosides leading to D-glucosides. Probably functions in a metabolic pathway that transforms D-gulosides to D-glucosides. Can use 3-dehydro-D-glucose, methyl alpha-3-dehydro-D-glucoside and methyl beta-3-dehydro-D-glucoside as substrates in vitro. However, the actual specific physiological substrates for this metabolic pathway are unknown. To a lesser extent, is also able to catalyze the reverse reactions, i.e. the NAD+-dependent oxidation of the hydroxyl group at C3 of D-glucosides leading to 3-dehydro-D-glucosides. Cannot act on UDP-glucose, UDP-N-acetyl-D-glucosamine, D-glucosamine, N-acetyl-D-glucosamine, or UDP-D-galactose.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

kcat is 0.65 sec(-1) for the NAD+-dependent oxidation of D-glucose (at pH 8.0 and 30 degrees Celsius). kcat is 1.14 sec(-1) for the NAD+-dependent oxidation of D-glucose (at pH 9.0 and 30 degrees Celsius). kcat is 0.58 sec(-1) for the NAD+-dependent oxidation of methyl alpha-D-glucoside (at pH 8.0 and 30 degrees Celsius). kcat is 1.2 sec(-1) for the NAD+-dependent oxidation of methyl alpha-D-glucoside (at pH 9.0 and 30 degrees Celsius). kcat is 0.4 sec(-1) for the NAD+-dependent oxidation of methyl beta-D-glucoside (at pH 8.0 and 30 degrees Celsius). kcat is 0.3 sec(-1) for the NAD+-dependent oxidation of methyl beta-D-glucoside (at pH 9.0 and 30 degrees Celsius). kcat is 0.17 sec(-1) for the NAD+-dependent oxidation of 1,5-anhydro-D-glucitol (at pH 8.0 and 30 degrees Celsius). kcat is 0.4 sec(-1) for the NAD+-dependent oxidation of 1,5-anhydro-D-glucitol (at pH 9.0 and 30 degrees Celsius). kcat is 4.7 sec(-1) for the NADH-dependent reduction of 3-dehydro-D-glucose (at pH 6.5 and 30 degrees Celsius). kcat is 22 sec(-1) for the NADH-dependent reduction of methyl alpha-3-dehydro-D-glucoside (at pH 7.0 and 30 degrees Celsius). kcat is 18 sec(-1) for the NADH-dependent reduction of methyl alpha-3-dehydro-D-glucoside (at pH 8.0 and 30 degrees Celsius). kcat is 19 sec(-1) for the NADH-dependent reduction of methyl beta-3-dehydro-D-glucoside (at pH 7.0 and 30 degrees Celsius). kcat is 12.5 sec(-1) for the NADH-dependent reduction of methyl beta-3-dehydro-D-glucoside (at pH 8.0 and 30 degrees Celsius).1 Publication
  1. KM=7.2 mM for D-glucose (at pH 8.0 and 30 degrees Celsius)1 Publication
  2. KM=8.9 mM for D-glucose (at pH 9.0 and 30 degrees Celsius)1 Publication
  3. KM=5.6 mM for methyl alpha-D-glucoside (at pH 8.0 and 30 degrees Celsius)1 Publication
  4. KM=5.9 mM for methyl alpha-D-glucoside (at pH 9.0 and 30 degrees Celsius)1 Publication
  5. KM=2.0 mM for methyl beta-D-glucoside (at pH 8.0 and 30 degrees Celsius)1 Publication
  6. KM=1.6 mM for methyl beta-D-glucoside (at pH 9.0 and 30 degrees Celsius)1 Publication
  7. KM=2.1 mM for 1,5-anhydro-D-glucitol (at pH 8.0 and 30 degrees Celsius)1 Publication
  8. KM=3.9 mM for 1,5-anhydro-D-glucitol (at pH 9.0 and 30 degrees Celsius)1 Publication
  9. KM=0.5 mM for 3-dehydro-D-glucose (at pH 6.5 and 30 degrees Celsius)1 Publication
  10. KM=0.95 mM for methyl alpha-3-dehydro-D-glucoside (at pH 7.0 and 30 degrees Celsius)1 Publication
  11. KM=1.2 mM for methyl alpha-3-dehydro-D-glucoside (at pH 8.0 and 30 degrees Celsius)1 Publication
  12. KM=0.80 mM for methyl beta-3-dehydro-D-glucoside (at pH 7.0 and 30 degrees Celsius)1 Publication
  13. KM=0.92 mM for methyl beta-3-dehydro-D-glucoside (at pH 8.0 and 30 degrees Celsius)1 Publication

    <p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

    GO - Biological processi

    <p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

    Molecular functionOxidoreductase
    Biological processCarbohydrate metabolism
    LigandNAD

    Enzyme and pathway databases

    BioCyc Collection of Pathway/Genome Databases

    More...
    BioCyci
    EcoCyc:G6653-MONOMER
    MetaCyc:G6653-MONOMER

    <p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
    Recommended name:
    D-glucoside 3-dehydrogenase1 Publication (EC:1.1.1.-1 Publication)
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
    Name:ycjS
    Ordered Locus Names:b1315, JW1308
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
    • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    <p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000917791 – 351D-glucoside 3-dehydrogenaseAdd BLAST351

    Proteomic databases

    PaxDb, a database of protein abundance averages across all three domains of life

    More...
    PaxDbi
    P77503

    PRoteomics IDEntifications database

    More...
    PRIDEi
    P77503

    <p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

    Protein-protein interaction databases

    The Biological General Repository for Interaction Datasets (BioGRID)

    More...
    BioGRIDi
    4263193, 12 interactors

    Protein interaction database and analysis system

    More...
    IntActi
    P77503, 1 interactor

    STRING: functional protein association networks

    More...
    STRINGi
    511145.b1315

    <p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

    3D structure databases

    SWISS-MODEL Repository - a database of annotated 3D protein structure models

    More...
    SMRi
    P77503

    Database of comparative protein structure models

    More...
    ModBasei
    Search...

    <p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

    <p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

    Belongs to the Gfo/Idh/MocA family.Curated

    Phylogenomic databases

    evolutionary genealogy of genes: Non-supervised Orthologous Groups

    More...
    eggNOGi
    COG0673, Bacteria

    The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

    More...
    HOGENOMi
    CLU_023194_1_4_6

    InParanoid: Eukaryotic Ortholog Groups

    More...
    InParanoidi
    P77503

    Database for complete collections of gene phylogenies

    More...
    PhylomeDBi
    P77503

    Family and domain databases

    Integrated resource of protein families, domains and functional sites

    More...
    InterProi
    View protein in InterPro
    IPR004104, Gfo/Idh/MocA-like_OxRdtase_C
    IPR036291, NAD(P)-bd_dom_sf
    IPR000683, Oxidoreductase_N

    Pfam protein domain database

    More...
    Pfami
    View protein in Pfam
    PF01408, GFO_IDH_MocA, 1 hit
    PF02894, GFO_IDH_MocA_C, 1 hit

    Superfamily database of structural and functional annotation

    More...
    SUPFAMi
    SSF51735, SSF51735, 1 hit

    <p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

    <p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

    P77503-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MKSAMTSSPL RVAIIGAGQV ADKVHASYYC TRNDLELVAV CDSRLSQAQA
    60 70 80 90 100
    LAEKYGNASV WDDPQAMLLA VKPDVVSVCS PNRFHYEHTL MALEAGCHVM
    110 120 130 140 150
    CEKPPAMTPE QAREMCDTAR KLGKVLAYDF HHRFALDTQQ LREQVTNGVL
    160 170 180 190 200
    GEIYVTTARA LRRCGVPGWG VFTNKELQGG GPLIDIGIHM LDAAMYVLGF
    210 220 230 240 250
    PAVKSVNAHS FQKIGTQKSC GQFGEWDPAT YSVEDSLFGT IEFHNGGILW
    260 270 280 290 300
    LETSFALNIR EQSIMNVSFC GDKAGATLFP AHIYTDNNGE LMTLMQREIA
    310 320 330 340 350
    DDNRHLRSME AFINHVQGKP VMIADAEQGY IIQQLVAALY QSAETGTRVE

    L
    Length:351
    Mass (Da):38,652
    Last modified:February 1, 1997 - v1
    <p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i3410735BAA0A756D
    GO

    Sequence databases

    Select the link destinations:

    EMBL nucleotide sequence database

    More...
    EMBLi

    GenBank nucleotide sequence database

    More...
    GenBanki

    DNA Data Bank of Japan; a nucleotide sequence database

    More...
    DDBJi
    Links Updated
    U00096 Genomic DNA Translation: AAC74397.1
    AP009048 Genomic DNA Translation: BAA14890.1

    Protein sequence database of the Protein Information Resource

    More...
    PIRi
    F64880

    NCBI Reference Sequences

    More...
    RefSeqi
    NP_415831.1, NC_000913.3
    WP_001395397.1, NZ_LN832404.1

    Genome annotation databases

    Ensembl bacterial and archaeal genome annotation project

    More...
    EnsemblBacteriai
    AAC74397; AAC74397; b1315
    BAA14890; BAA14890; BAA14890

    Database of genes from NCBI RefSeq genomes

    More...
    GeneIDi
    948589

    KEGG: Kyoto Encyclopedia of Genes and Genomes

    More...
    KEGGi
    ecj:JW1308
    eco:b1315

    Pathosystems Resource Integration Center (PATRIC)

    More...
    PATRICi
    fig|1411691.4.peg.964

    <p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

    <p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U00096 Genomic DNA Translation: AAC74397.1
    AP009048 Genomic DNA Translation: BAA14890.1
    PIRiF64880
    RefSeqiNP_415831.1, NC_000913.3
    WP_001395397.1, NZ_LN832404.1

    3D structure databases

    SMRiP77503
    ModBaseiSearch...

    Protein-protein interaction databases

    BioGRIDi4263193, 12 interactors
    IntActiP77503, 1 interactor
    STRINGi511145.b1315

    Proteomic databases

    PaxDbiP77503
    PRIDEiP77503

    Genome annotation databases

    EnsemblBacteriaiAAC74397; AAC74397; b1315
    BAA14890; BAA14890; BAA14890
    GeneIDi948589
    KEGGiecj:JW1308
    eco:b1315
    PATRICifig|1411691.4.peg.964

    Organism-specific databases

    EchoBASE - an integrated post-genomic database for E. coli

    More...
    EchoBASEi
    EB3675

    Phylogenomic databases

    eggNOGiCOG0673, Bacteria
    HOGENOMiCLU_023194_1_4_6
    InParanoidiP77503
    PhylomeDBiP77503

    Enzyme and pathway databases

    BioCyciEcoCyc:G6653-MONOMER
    MetaCyc:G6653-MONOMER

    Miscellaneous databases

    Protein Ontology

    More...
    PROi
    PR:P77503

    Family and domain databases

    InterProiView protein in InterPro
    IPR004104, Gfo/Idh/MocA-like_OxRdtase_C
    IPR036291, NAD(P)-bd_dom_sf
    IPR000683, Oxidoreductase_N
    PfamiView protein in Pfam
    PF01408, GFO_IDH_MocA, 1 hit
    PF02894, GFO_IDH_MocA_C, 1 hit
    SUPFAMiSSF51735, SSF51735, 1 hit

    ProtoNet; Automatic hierarchical classification of proteins

    More...
    ProtoNeti
    Search...

    MobiDB: a database of protein disorder and mobility annotations

    More...
    MobiDBi
    Search...

    <p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

    <p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiYCJS_ECOLI
    <p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P77503
    <p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: February 1, 1997
    Last modified: December 2, 2020
    This is version 125 of the entry and version 1 of the sequence. See complete history.
    <p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    <p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

    Keywords - Technical termi

    Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
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