UniProtKB - P77494 (HOKB_ECOLI)
Protein
Toxic protein HokB
Gene
hokB
Organism
Escherichia coli (strain K12)
Status
Functioni
Toxic component of a type I toxin-antitoxin (TA) system (Probable). When overexpressed kills cells within minutes; causes collapse of the transmembrane potential and arrest of respiration (PubMed:10361310). Expression leads to membrane depolarization; when protein levels are high enough depolarization probably leads to lowered metabolic activity which in turn induces some cells to enter the persistent state in which they transiently survive antibiotic exposure. Its toxic effect is probably neutralized by antisense antitoxin RNA SokB, which is encoded in trans on the opposite DNA strand (PubMed:10361310).1 Publication2 Publications
GO - Biological processi
- dormancy process Source: UniProtKB
Keywordsi
Biological process | Toxin-antitoxin system |
Enzyme and pathway databases
BioCyci | EcoCyc:MONOMER0-1604 |
Protein family/group databases
TCDBi | 1.E.53.1.3, the toxic hok/gef protein (hok/gef) family |
Names & Taxonomyi
Protein namesi | Recommended name: Toxic protein HokBCurated |
Gene namesi | Name:hokB1 Publication Synonyms:ydcB Ordered Locus Names:b4428, JW5225 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane By similarity1 Publication; Single-pass membrane protein By similarity
Plasma Membrane
- integral component of plasma membrane Source: EcoCyc
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 4 – 24 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Partial loss of Obg-controlled persistence.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000199030 | 1 – 49 | Toxic protein HokBAdd BLAST | 49 |
Proteomic databases
PaxDbi | P77494 |
PRIDEi | P77494 |
Expressioni
Inductioni
Induced by GTPase Obg expression, which requires alarmone (p)ppGpp.1 Publication
Interactioni
Protein-protein interaction databases
BioGRIDi | 4262884, 12 interactors |
STRINGi | 511145.b4428 |
Family & Domainsi
Sequence similaritiesi
Belongs to the Hok/Gef family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG50339QC, Bacteria |
HOGENOMi | CLU_177638_2_1_6 |
Family and domain databases
InterProi | View protein in InterPro IPR000021, Hok/gef_toxin IPR018084, Hok/gef_toxin_CS |
Pfami | View protein in Pfam PF01848, HOK_GEF, 1 hit |
PRINTSi | PR00281, HOKGEFTOXIC |
PROSITEi | View protein in PROSITE PS00556, HOK_GEF, 1 hit |
i Sequence
Sequence statusi: Complete.
P77494-1 [UniParc]FASTAAdd to basket
10 20 30 40
MKHNPLVVCL LIICITILTF TLLTRQTLYE LRFRDGDKEV AALMACTSR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAT48130.1 AP009048 Genomic DNA Translation: BAA15042.2 |
RefSeqi | WP_001320773.1, NZ_SSZK01000021.1 YP_025301.1, NC_000913.3 |
Genome annotation databases
EnsemblBacteriai | AAT48130; AAT48130; b4428 BAA15042; BAA15042; BAA15042 |
GeneIDi | 2847727 57732154 |
KEGGi | ecj:JW5225 eco:b4428 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAT48130.1 AP009048 Genomic DNA Translation: BAA15042.2 |
RefSeqi | WP_001320773.1, NZ_SSZK01000021.1 YP_025301.1, NC_000913.3 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 4262884, 12 interactors |
STRINGi | 511145.b4428 |
Protein family/group databases
TCDBi | 1.E.53.1.3, the toxic hok/gef protein (hok/gef) family |
Proteomic databases
PaxDbi | P77494 |
PRIDEi | P77494 |
Genome annotation databases
EnsemblBacteriai | AAT48130; AAT48130; b4428 BAA15042; BAA15042; BAA15042 |
GeneIDi | 2847727 57732154 |
KEGGi | ecj:JW5225 eco:b4428 |
Organism-specific databases
EchoBASEi | EB1222 |
Phylogenomic databases
eggNOGi | ENOG50339QC, Bacteria |
HOGENOMi | CLU_177638_2_1_6 |
Enzyme and pathway databases
BioCyci | EcoCyc:MONOMER0-1604 |
Miscellaneous databases
PROi | PR:P77494 |
Family and domain databases
InterProi | View protein in InterPro IPR000021, Hok/gef_toxin IPR018084, Hok/gef_toxin_CS |
Pfami | View protein in Pfam PF01848, HOK_GEF, 1 hit |
PRINTSi | PR00281, HOKGEFTOXIC |
PROSITEi | View protein in PROSITE PS00556, HOK_GEF, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HOKB_ECOLI | |
Accessioni | P77494Primary (citable) accession number: P77494 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | July 15, 1998 | |
Last modified: | February 10, 2021 | |
This is version 113 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families