UniProtKB - P77485 (CUSS_ECOLI)
Protein
Sensor histidine kinase CusS
Gene
cusS
Organism
Escherichia coli (strain K12)
Status
Functioni
Member of the two-component regulatory system CusS/CusR involved in response to copper and silver. Acts as a copper/silver ion sensor. Activates CusR by phosphorylation.5 Publications
Miscellaneous
The cus system plays an important role in copper tolerance under anaerobic growth and, under extreme copper stress, in aerobic growth.1 Publication
HprSR and CusSR form a unique regulation system, where both two-component systems recognize and regulate the same set of genes, but under different environmental conditions. HprSR plays a role in H2O2 response regulation, while CusSR plays a role in Cu2+ response regulation.2 Publications
Catalytic activityi
- ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.1 Publication EC:2.7.13.3
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- metal ion binding Source: EcoCyc
- phosphorelay sensor kinase activity Source: EcoliWiki
- protein histidine kinase activity Source: EcoCyc
GO - Biological processi
- cellular response to copper ion Source: EcoCyc
- cellular response to silver ion Source: EcoCyc
- peptidyl-histidine phosphorylation Source: EcoCyc
- phosphorelay signal transduction system Source: GO_Central
- phosphorylation Source: EcoliWiki
- signal transduction by protein phosphorylation Source: EcoCyc
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Two-component regulatory system |
Ligand | ATP-binding, Copper, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:G6318-MONOMER MetaCyc:G6318-MONOMER |
BRENDAi | 2.7.13.3, 2026 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:cusS Synonyms:ybcZ Ordered Locus Names:b0570, JW5082 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cell inner membrane 1 Publication; Multi-pass membrane protein Sequence analysis
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 15 | CytoplasmicCuratedAdd BLAST | 15 | |
Transmembranei | 16 – 36 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 37 – 186 | PeriplasmicCuratedAdd BLAST | 150 | |
Transmembranei | 187 – 207 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 208 – 480 | Cytoplasmic1 PublicationAdd BLAST | 273 |
GO - Cellular componenti
- integral component of plasma membrane Source: EcoCyc
- membrane Source: EcoliWiki
- plasma membrane Source: EcoCyc
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Deletion mutant has higher susceptibility to silver and shows an increase in copper accumulation.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000074724 | 1 – 480 | Sensor histidine kinase CusSAdd BLAST | 480 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 271 | Phosphohistidine; by autocatalysisPROSITE-ProRule annotation | 1 |
Post-translational modificationi
Autophosphorylated.1 Publication
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | P77485 |
PRIDEi | P77485 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4259499, 18 interactors 850340, 1 interactor |
DIPi | DIP-9349N |
IntActi | P77485, 2 interactors |
STRINGi | 511145.b0570 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P77485 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 208 – 260 | HAMPPROSITE-ProRule annotationAdd BLAST | 53 | |
Domaini | 268 – 480 | Histidine kinasePROSITE-ProRule annotationAdd BLAST | 213 |
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG5002, Bacteria |
HOGENOMi | CLU_000445_89_6_6 |
InParanoidi | P77485 |
PhylomeDBi | P77485 |
Family and domain databases
CDDi | cd06225, HAMP, 1 hit cd00082, HisKA, 1 hit |
Gene3Di | 3.30.565.10, 1 hit |
InterProi | View protein in InterPro IPR006290, CztS_silS_copS IPR003660, HAMP_dom IPR003594, HATPase_C IPR036890, HATPase_C_sf IPR005467, His_kinase_dom IPR003661, HisK_dim/P IPR036097, HisK_dim/P_sf IPR004358, Sig_transdc_His_kin-like_C |
Pfami | View protein in Pfam PF00672, HAMP, 1 hit PF02518, HATPase_c, 1 hit PF00512, HisKA, 1 hit |
PRINTSi | PR00344, BCTRLSENSOR |
SMARTi | View protein in SMART SM00304, HAMP, 1 hit SM00387, HATPase_c, 1 hit SM00388, HisKA, 1 hit |
SUPFAMi | SSF47384, SSF47384, 1 hit SSF55874, SSF55874, 1 hit |
TIGRFAMsi | TIGR01386, cztS_silS_copS, 1 hit |
PROSITEi | View protein in PROSITE PS50885, HAMP, 1 hit PS50109, HIS_KIN, 1 hit |
i Sequence
Sequence statusi: Complete.
P77485-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVSKPFQRPF SLATRLTFFI SLATIAAFFA FAWIMIHSVK VHFAEQDIND
60 70 80 90 100
LKEISATLER VLNHPDETQA RRLMTLEDIV SGYSNVLISL ADSQGKTVYH
110 120 130 140 150
SPGAPDIREF TRDAIPDKDA QGGEVYLLSG PTMMMPGHGH GHMEHSNWRM
160 170 180 190 200
INLPVGPLVD GKPIYTLYIA LSIDFHLHYI NDLMNKLIMT ASVISILIVF
210 220 230 240 250
IVLLAVHKGH APIRSVSRQI QNITSKDLDV RLDPQTVPIE LEQLVLSFNH
260 270 280 290 300
MIERIEDVFT RQSNFSADIA HEIRTPITNL ITQTEIALSQ SRSQKELEDV
310 320 330 340 350
LYSNLEELTR MAKMVSDMLF LAQADNNQLI PEKKMLNLAD EVGKVFDFFE
360 370 380 390 400
ALAEDRGVEL RFVGDKCQVA GDPLMLRRAL SNLLSNALRY TPTGETIVVR
410 420 430 440 450
CQTVDHLVQV IVENPGTPIA PEHLPRLFDR FYRVDPSRQR KGEGSGIGLA
460 470 480
IVKSIVVAHK GTVAVTSDAR GTRFVITLPA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF245661 Genomic DNA Translation: AAF70176.1 U82598 Genomic DNA Translation: AAB40768.1 U00096 Genomic DNA Translation: AAC73671.1 AP009048 Genomic DNA Translation: BAA35204.2 |
PIRi | H64789 |
RefSeqi | NP_415102.1, NC_000913.3 WP_000253839.1, NZ_SSZK01000024.1 |
Genome annotation databases
EnsemblBacteriai | AAC73671; AAC73671; b0570 BAA35204; BAA35204; BAA35204 |
GeneIDi | 945978 |
KEGGi | ecj:JW5082 eco:b0570 |
PATRICi | fig|1411691.4.peg.1704 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF245661 Genomic DNA Translation: AAF70176.1 U82598 Genomic DNA Translation: AAB40768.1 U00096 Genomic DNA Translation: AAC73671.1 AP009048 Genomic DNA Translation: BAA35204.2 |
PIRi | H64789 |
RefSeqi | NP_415102.1, NC_000913.3 WP_000253839.1, NZ_SSZK01000024.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5KU5 | X-ray | 2.15 | A/B/C/D | 39-187 | [»] | |
SMRi | P77485 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259499, 18 interactors 850340, 1 interactor |
DIPi | DIP-9349N |
IntActi | P77485, 2 interactors |
STRINGi | 511145.b0570 |
Proteomic databases
PaxDbi | P77485 |
PRIDEi | P77485 |
Genome annotation databases
EnsemblBacteriai | AAC73671; AAC73671; b0570 BAA35204; BAA35204; BAA35204 |
GeneIDi | 945978 |
KEGGi | ecj:JW5082 eco:b0570 |
PATRICi | fig|1411691.4.peg.1704 |
Organism-specific databases
EchoBASEi | EB3406 |
Phylogenomic databases
eggNOGi | COG5002, Bacteria |
HOGENOMi | CLU_000445_89_6_6 |
InParanoidi | P77485 |
PhylomeDBi | P77485 |
Enzyme and pathway databases
BioCyci | EcoCyc:G6318-MONOMER MetaCyc:G6318-MONOMER |
BRENDAi | 2.7.13.3, 2026 |
Miscellaneous databases
PROi | PR:P77485 |
Family and domain databases
CDDi | cd06225, HAMP, 1 hit cd00082, HisKA, 1 hit |
Gene3Di | 3.30.565.10, 1 hit |
InterProi | View protein in InterPro IPR006290, CztS_silS_copS IPR003660, HAMP_dom IPR003594, HATPase_C IPR036890, HATPase_C_sf IPR005467, His_kinase_dom IPR003661, HisK_dim/P IPR036097, HisK_dim/P_sf IPR004358, Sig_transdc_His_kin-like_C |
Pfami | View protein in Pfam PF00672, HAMP, 1 hit PF02518, HATPase_c, 1 hit PF00512, HisKA, 1 hit |
PRINTSi | PR00344, BCTRLSENSOR |
SMARTi | View protein in SMART SM00304, HAMP, 1 hit SM00387, HATPase_c, 1 hit SM00388, HisKA, 1 hit |
SUPFAMi | SSF47384, SSF47384, 1 hit SSF55874, SSF55874, 1 hit |
TIGRFAMsi | TIGR01386, cztS_silS_copS, 1 hit |
PROSITEi | View protein in PROSITE PS50885, HAMP, 1 hit PS50109, HIS_KIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CUSS_ECOLI | |
Accessioni | P77485Primary (citable) accession number: P77485 Secondary accession number(s): Q9R7T9, Q9R7U0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | February 1, 1997 | |
Last modified: | December 2, 2020 | |
This is version 179 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references