UniProtKB - P77467 (PAAG_ECOLI)
Protein
1,2-epoxyphenylacetyl-CoA isomerase
Gene
paaG
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the reversible conversion of the epoxide to 2-oxepin-2(3H)-ylideneacetyl-CoA (oxepin-CoA).3 Publications
Catalytic activityi
- EC:5.3.3.18
: phenylacetate degradation Pathwayi
This protein is involved in the pathway phenylacetate degradation, which is part of Aromatic compound metabolism.View all proteins of this organism that are known to be involved in the pathway phenylacetate degradation and in Aromatic compound metabolism.
GO - Molecular functioni
- enoyl-CoA hydratase activity Source: GO_Central
- identical protein binding Source: EcoCyc
- isomerase activity Source: UniProtKB
GO - Biological processi
- fatty acid beta-oxidation Source: GO_Central
- phenylacetate catabolic process Source: EcoCyc
Keywordsi
Molecular function | Isomerase, Lyase |
Enzyme and pathway databases
BioCyci | EcoCyc:G6715-MONOMER |
BRENDAi | 5.3.3.18, 2026 |
UniPathwayi | UPA00930 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:paaG Synonyms:ydbT Ordered Locus Names:b1394, JW1389 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Mutants accumulate ring-1,2-dihydroxy-1,2-dihydrophenylacetyl lactone and are unable to use phenylacetate as a carbon source.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000109331 | 1 – 262 | 1,2-epoxyphenylacetyl-CoA isomeraseAdd BLAST | 262 |
Proteomic databases
PaxDbi | P77467 |
PRIDEi | P77467 |
Expressioni
Inductioni
Activated by cAMP receptor protein (CRP), integration host factor (IHF) and by phenylacetyl-coenzyme A (PA-CoA) that prevents PaaX from binding its target sequences. Inhibited by PaaX.2 Publications
Interactioni
Binary interactionsi
P77467
With | #Exp. | IntAct |
---|---|---|
paaF [P76082] | 3 | EBI-1118484,EBI-1115747 |
GO - Molecular functioni
- identical protein binding Source: EcoCyc
Protein-protein interaction databases
BioGRIDi | 4260969, 375 interactors 850622, 7 interactors |
ComplexPortali | CPX-5681, Enoyl CoA hydratase/isomerase complex |
IntActi | P77467, 10 interactors |
STRINGi | 511145.b1394 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P77467 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the enoyl-CoA hydratase/isomerase family.Curated
Phylogenomic databases
eggNOGi | COG1024, Bacteria |
HOGENOMi | CLU_009834_7_2_6 |
InParanoidi | P77467 |
PhylomeDBi | P77467 |
Family and domain databases
Gene3Di | 1.10.12.10, 1 hit |
InterProi | View protein in InterPro IPR029045, ClpP/crotonase-like_dom_sf IPR001753, Enoyl-CoA_hydra/iso IPR014748, Enoyl-CoA_hydra_C IPR011968, PaaB1 |
Pfami | View protein in Pfam PF00378, ECH_1, 1 hit |
SUPFAMi | SSF52096, SSF52096, 1 hit |
TIGRFAMsi | TIGR02280, PaaB1, 1 hit |
i Sequence
Sequence statusi: Complete.
P77467-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MMEFILSHVE KGVMTLTLNR PERLNSFNDE MHAQLAECLK QVERDDTIRC
60 70 80 90 100
LLLTGAGRGF CAGQDLNDRN VDPTGPAPDL GMSVERFYNP LVRRLAKLPK
110 120 130 140 150
PVICAVNGVA AGAGATLALG GDIVIAARSA KFVMAFSKLG LIPDCGGTWL
160 170 180 190 200
LPRVAGRARA MGLALLGNQL SAEQAHEWGM IWQVVDDETL ADTAQQLARH
210 220 230 240 250
LATQPTFGLG LIKQAINSAE TNTLDTQLDL ERDYQRLAGR SADYREGVSA
260
FLAKRSPQFT GK
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 121 | G → C in strain: W. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X97452 Genomic DNA Translation: CAA66096.1 U00096 Genomic DNA Translation: AAC74476.1 AP009048 Genomic DNA Translation: BAA15000.1 |
PIRi | E64890 |
RefSeqi | NP_415912.1, NC_000913.3 WP_000969777.1, NZ_STEB01000005.1 WP_000969784.1, NZ_SSZK01000012.1 |
Genome annotation databases
EnsemblBacteriai | AAC74476; AAC74476; b1394 BAA15000; BAA15000; BAA15000 |
GeneIDi | 946263 |
KEGGi | ecj:JW1389 eco:b1394 |
PATRICi | fig|1411691.4.peg.877 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X97452 Genomic DNA Translation: CAA66096.1 U00096 Genomic DNA Translation: AAC74476.1 AP009048 Genomic DNA Translation: BAA15000.1 |
PIRi | E64890 |
RefSeqi | NP_415912.1, NC_000913.3 WP_000969777.1, NZ_STEB01000005.1 WP_000969784.1, NZ_SSZK01000012.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4FZW | X-ray | 2.55 | C/D | 1-262 | [»] | |
SMRi | P77467 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260969, 375 interactors 850622, 7 interactors |
ComplexPortali | CPX-5681, Enoyl CoA hydratase/isomerase complex |
IntActi | P77467, 10 interactors |
STRINGi | 511145.b1394 |
Proteomic databases
PaxDbi | P77467 |
PRIDEi | P77467 |
Genome annotation databases
EnsemblBacteriai | AAC74476; AAC74476; b1394 BAA15000; BAA15000; BAA15000 |
GeneIDi | 946263 |
KEGGi | ecj:JW1389 eco:b1394 |
PATRICi | fig|1411691.4.peg.877 |
Organism-specific databases
EchoBASEi | EB3504 |
Phylogenomic databases
eggNOGi | COG1024, Bacteria |
HOGENOMi | CLU_009834_7_2_6 |
InParanoidi | P77467 |
PhylomeDBi | P77467 |
Enzyme and pathway databases
UniPathwayi | UPA00930 |
BioCyci | EcoCyc:G6715-MONOMER |
BRENDAi | 5.3.3.18, 2026 |
Miscellaneous databases
PROi | PR:P77467 |
Family and domain databases
Gene3Di | 1.10.12.10, 1 hit |
InterProi | View protein in InterPro IPR029045, ClpP/crotonase-like_dom_sf IPR001753, Enoyl-CoA_hydra/iso IPR014748, Enoyl-CoA_hydra_C IPR011968, PaaB1 |
Pfami | View protein in Pfam PF00378, ECH_1, 1 hit |
SUPFAMi | SSF52096, SSF52096, 1 hit |
TIGRFAMsi | TIGR02280, PaaB1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PAAG_ECOLI | |
Accessioni | P77467Primary (citable) accession number: P77467 Secondary accession number(s): O53015 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | February 1, 1997 | |
Last modified: | April 7, 2021 | |
This is version 147 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families