UniProtKB - P77455 (PAAZ_ECOLI)
Protein
Bifunctional protein PaaZ
Gene
paaZ
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the hydrolytic ring cleavage of 2-oxepin-2(3H)-ylideneacetyl-CoA (oxepin-CoA) via the open-chain aldehyde intermediate to yield 3-oxo-5,6-dehydrosuberyl-CoA. The enzyme consists of a C-terminal (R)-specific enoyl-CoA hydratase domain (formerly MaoC) that cleaves the ring and produces the highly reactive 3-oxo-5,6-dehydrosuberyl-CoA semialdehyde and an N-terminal NADP-dependent aldehyde dehydrogenase domain that oxidizes the aldehyde to 3-oxo-5,6-dehydrosuberyl-CoA. Can also use crotonyl-CoA as substrate.3 Publications
Catalytic activityi
- EC:3.3.2.121 Publication
- 3-oxo-5,6-dehydrosuberyl-CoA semialdehyde + H2O + NADP+ = 3-oxo-5,6-didehydrosuberyl-CoA + 2 H+ + NADPH1 PublicationEC:1.2.1.911 Publication
Kineticsi
- KM=11 µM for oxepin-CoA (at 22 degrees Celsius and pH 8)1 Publication
- KM=56 µM for NADP (at 22 degrees Celsius and pH 8)1 Publication
pH dependencei
Optimum pH is 8.1 Publication
: phenylacetate degradation Pathwayi
This protein is involved in the pathway phenylacetate degradation, which is part of Aromatic compound metabolism.View all proteins of this organism that are known to be involved in the pathway phenylacetate degradation and in Aromatic compound metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 256 | By similarity | 1 | |
Active sitei | 295 | By similarity | 1 |
GO - Molecular functioni
- enoyl-CoA hydratase activity Source: EcoCyc
- ether hydrolase activity Source: EcoCyc
- hydrolase activity, acting on acid carbon-carbon bonds, in ketonic substances Source: UniProtKB
- oxidoreductase activity, acting on CH or CH2 groups, NAD or NADP as acceptor Source: UniProtKB
- oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor Source: InterPro
GO - Biological processi
- phenylacetate catabolic process Source: UniProtKB
Keywordsi
Molecular function | Hydrolase, Lyase, Multifunctional enzyme, Oxidoreductase |
Ligand | NADP |
Enzyme and pathway databases
BioCyci | EcoCyc:G6708-MONOMER MetaCyc:G6708-MONOMER |
BRENDAi | 3.3.2.12, 2026 |
UniPathwayi | UPA00930 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:paaZ Synonyms:maoC, ydbN Ordered Locus Names:b1387, JW1382 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Disruption causes the conversion of phenylacetate (PA) into 2-hydroxyphenylacetate (2-HPA).1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 256 | E → Q: Catalyzes the formation of 3-oxo-5,6-dehydrosuberyl-CoA semialdehyde instead of 3-oxo-5,6-dehydrosuberyl-CoA. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000056581 | 1 – 681 | Bifunctional protein PaaZAdd BLAST | 681 |
Proteomic databases
PaxDbi | P77455 |
PRIDEi | P77455 |
Expressioni
Inductioni
Activated by cAMP receptor protein (CRP) and integration host factor (IHF). Inhibited by PaaX.1 Publication
Interactioni
Protein-protein interaction databases
BioGRIDi | 4260176, 262 interactors |
IntActi | P77455, 3 interactors |
STRINGi | 511145.b1387 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SASBDBi | P77455 |
SMRi | P77455 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 537 – 648 | MaoC-likeAdd BLAST | 112 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 333 | Aldehyde dehydrogenaseAdd BLAST | 333 |
Sequence similaritiesi
In the N-terminal section; belongs to the aldehyde dehydrogenase family.Curated
Phylogenomic databases
eggNOGi | COG1012, Bacteria COG2030, Bacteria |
HOGENOMi | CLU_025047_0_0_6 |
InParanoidi | P77455 |
PhylomeDBi | P77455 |
Family and domain databases
Gene3Di | 3.40.309.10, 1 hit 3.40.605.10, 1 hit |
InterProi | View protein in InterPro IPR016161, Ald_DH/histidinol_DH IPR016163, Ald_DH_C IPR016162, Ald_DH_N IPR015590, Aldehyde_DH_dom IPR029069, HotDog_dom_sf IPR002539, MaoC-like_dom IPR011966, PaaN-DH |
Pfami | View protein in Pfam PF00171, Aldedh, 1 hit PF01575, MaoC_dehydratas, 1 hit |
SUPFAMi | SSF53720, SSF53720, 1 hit SSF54637, SSF54637, 1 hit |
TIGRFAMsi | TIGR02278, PaaN-DH, 1 hit |
i Sequence
Sequence statusi: Complete.
P77455-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQQLASFLSG TWQSGRGRSR LIHHAISGEA LWEVTSEGLD MAAARQFAIE
60 70 80 90 100
KGAPALRAMT FIERAAMLKA VAKHLLSEKE RFYALSAQTG ATRADSWVDI
110 120 130 140 150
EGGIGTLFTY ASLGSRELPD DTLWPEDELI PLSKEGGFAA RHLLTSKSGV
160 170 180 190 200
AVHINAFNFP CWGMLEKLAP TWLGGMPAII KPATATAQLT QAMVKSIVDS
210 220 230 240 250
GLVPEGAISL ICGSAGDLLD HLDSQDVVTF TGSAATGQML RVQPNIVAKS
260 270 280 290 300
IPFTMEADSL NCCVLGEDVT PDQPEFALFI REVVREMTTK AGQKCTAIRR
310 320 330 340 350
IIVPQALVNA VSDALVARLQ KVVVGDPAQE GVKMGALVNA EQRADVQEKV
360 370 380 390 400
NILLAAGCEI RLGGQADLSA AGAFFPPTLL YCPQPDETPA VHATEAFGPV
410 420 430 440 450
ATLMPAQNQR HALQLACAGG GSLAGTLVTA DPQIARQFIA DAARTHGRIQ
460 470 480 490 500
ILNEESAKES TGHGSPLPQL VHGGPGRAGG GEELGGLRAV KHYMQRTAVQ
510 520 530 540 550
GSPTMLAAIS KQWVRGAKVE EDRIHPFRKY FEELQPGDSL LTPRRTMTEA
560 570 580 590 600
DIVNFACLSG DHFYAHMDKI AAAESIFGER VVHGYFVLSA AAGLFVDAGV
610 620 630 640 650
GPVIANYGLE SLRFIEPVKP GDTIQVRLTC KRKTLKKQRS AEEKPTGVVE
660 670 680
WAVEVFNQHQ TPVALYSILT LVARQHGDFV D
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 143 | L → V in strain: W. | 1 | |
Natural varianti | 440 | A → T in strain: W. | 1 | |
Natural varianti | 611 | S → N in strain: W. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X97452 Genomic DNA Translation: CAA66089.1 U00096 Genomic DNA Translation: AAC74469.1 AP009048 Genomic DNA Translation: BAA14997.1 |
PIRi | F64889 |
RefSeqi | NP_415905.1, NC_000913.3 WP_001186469.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC74469; AAC74469; b1387 BAA14997; BAA14997; BAA14997 |
GeneIDi | 945954 |
KEGGi | ecj:JW1382 eco:b1387 |
PATRICi | fig|1411691.4.peg.884 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X97452 Genomic DNA Translation: CAA66089.1 U00096 Genomic DNA Translation: AAC74469.1 AP009048 Genomic DNA Translation: BAA14997.1 |
PIRi | F64889 |
RefSeqi | NP_415905.1, NC_000913.3 WP_001186469.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6JQL | electron microscopy | 2.90 | A/B/C/D/E/F | 2-681 | [»] | |
6JQM | electron microscopy | 3.30 | A/B/C/D/E/F | 2-681 | [»] | |
6JQN | electron microscopy | 3.10 | A/B/C/D/E/F | 2-681 | [»] | |
6JQO | electron microscopy | 3.10 | A/B/C/D/E/F | 2-681 | [»] | |
SASBDBi | P77455 | |||||
SMRi | P77455 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260176, 262 interactors |
IntActi | P77455, 3 interactors |
STRINGi | 511145.b1387 |
Proteomic databases
PaxDbi | P77455 |
PRIDEi | P77455 |
Genome annotation databases
EnsemblBacteriai | AAC74469; AAC74469; b1387 BAA14997; BAA14997; BAA14997 |
GeneIDi | 945954 |
KEGGi | ecj:JW1382 eco:b1387 |
PATRICi | fig|1411691.4.peg.884 |
Organism-specific databases
EchoBASEi | EB3498 |
Phylogenomic databases
eggNOGi | COG1012, Bacteria COG2030, Bacteria |
HOGENOMi | CLU_025047_0_0_6 |
InParanoidi | P77455 |
PhylomeDBi | P77455 |
Enzyme and pathway databases
UniPathwayi | UPA00930 |
BioCyci | EcoCyc:G6708-MONOMER MetaCyc:G6708-MONOMER |
BRENDAi | 3.3.2.12, 2026 |
Miscellaneous databases
PROi | PR:P77455 |
Family and domain databases
Gene3Di | 3.40.309.10, 1 hit 3.40.605.10, 1 hit |
InterProi | View protein in InterPro IPR016161, Ald_DH/histidinol_DH IPR016163, Ald_DH_C IPR016162, Ald_DH_N IPR015590, Aldehyde_DH_dom IPR029069, HotDog_dom_sf IPR002539, MaoC-like_dom IPR011966, PaaN-DH |
Pfami | View protein in Pfam PF00171, Aldedh, 1 hit PF01575, MaoC_dehydratas, 1 hit |
SUPFAMi | SSF53720, SSF53720, 1 hit SSF54637, SSF54637, 1 hit |
TIGRFAMsi | TIGR02278, PaaN-DH, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PAAZ_ECOLI | |
Accessioni | P77455Primary (citable) accession number: P77455 Secondary accession number(s): O53009 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | February 1, 1997 | |
Last modified: | December 2, 2020 | |
This is version 149 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families