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Protein

Cysteine desulfurase

Gene

sufS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cysteine desulfurases mobilize the sulfur from L-cysteine to yield L-alanine, an essential step in sulfur metabolism for biosynthesis of a variety of sulfur-containing biomolecules. Component of the suf operon, which is activated and required under specific conditions such as oxidative stress and iron limitation. Acts as a potent selenocysteine lyase in vitro, that mobilizes selenium from L-selenocysteine. Selenocysteine lyase activity is however unsure in vivo.5 Publications

Catalytic activityi

L-cysteine + acceptor = L-alanine + S-sulfanyl-acceptor.
L-selenocysteine + reduced acceptor = selenide + L-alanine + acceptor.

Cofactori

Activity regulationi

Displays a strong preference for selenocysteine as a substrate in vitro and is only very slightly active using cysteine. The interactions with SufE and the SufBCD complex act synergistically to enhance, up to 50-fold, its cysteine desulfurase activity.3 Publications

Pathwayi: iron-sulfur cluster biosynthesis

This protein is involved in the pathway iron-sulfur cluster biosynthesis, which is part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the pathway iron-sulfur cluster biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei364Cysteine persulfide intermediate1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase, Transferase
LigandPyridoxal phosphate

Enzyme and pathway databases

BioCyciEcoCyc:G6906-MONOMER
MetaCyc:G6906-MONOMER
BRENDAi2.8.1.7 2026
4.4.1.16 2026
UniPathwayi
UPA00266

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine desulfurase (EC:2.8.1.7)
Alternative name(s):
Selenocysteine beta-lyase
Short name:
SCL
Selenocysteine lyase (EC:4.4.1.16)
Selenocysteine reductase
Gene namesi
Name:sufS
Synonyms:csdB, ynhB
Ordered Locus Names:b1680, JW1670
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13962 sufS

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi55H → A: No effect. 1 Publication1
Mutagenesisi123H → A: Loss of function; possibly due to destabilization of PLP in the active site. 1 Publication1
Mutagenesisi364C → A: Abolishes activity towards L-cysteine but not towards selenocysteine. 1 Publication1
Mutagenesisi379R → A: Loss of function. 1 Publication1

Chemistry databases

DrugBankiDB04217 L-2-amino-3-butynoic acid
DB02761 S-Mercaptocysteine
DB03049 S-Selanyl Cysteine
DB02345 Selenocysteine

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001503291 – 406Cysteine desulfuraseAdd BLAST406

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei226N6-(pyridoxal phosphate)lysine1

Proteomic databases

EPDiP77444
PaxDbiP77444
PRIDEiP77444

Expressioni

Inductioni

Suf operon is under both the Fe-dependent Fur repressor and the oxidative stress dependent OxyR activator.

Interactioni

Subunit structurei

Homodimer. Interacts with SufE and the SufBCD complex composed of SufB, SufC and SufD. The interaction with SufE is required to mediate the direct transfer of the sulfur atom from the S-sulfanylcysteine.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
sufEP761943EBI-1124981,EBI-1124973

Protein-protein interaction databases

BioGridi4260281, 100 interactors
ComplexPortaliCPX-2124 SufS complex
DIPiDIP-9324N
IntActiP77444, 14 interactors
STRINGi316385.ECDH10B_1814

Structurei

Secondary structure

1406
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP77444
SMRiP77444
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP77444

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105C9B Bacteria
COG0520 LUCA
HOGENOMiHOG000017511
InParanoidiP77444
KOiK11717
PhylomeDBiP77444

Family and domain databases

Gene3Di3.40.640.10, 1 hit
3.90.1150.10, 1 hit
HAMAPiMF_01831 SufS_aminotrans_5, 1 hit
InterProiView protein in InterPro
IPR000192 Aminotrans_V_dom
IPR020578 Aminotrans_V_PyrdxlP_BS
IPR010970 Cys_dSase_SufS
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
PfamiView protein in Pfam
PF00266 Aminotran_5, 1 hit
SUPFAMiSSF53383 SSF53383, 1 hit
TIGRFAMsiTIGR01979 sufS, 1 hit
PROSITEiView protein in PROSITE
PS00595 AA_TRANSFER_CLASS_5, 1 hit

Sequencei

Sequence statusi: Complete.

P77444-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MIFSVDKVRA DFPVLSREVN GLPLAYLDSA ASAQKPSQVI DAEAEFYRHG
60 70 80 90 100
YAAVHRGIHT LSAQATEKME NVRKRASLFI NARSAEELVF VRGTTEGINL
110 120 130 140 150
VANSWGNSNV RAGDNIIISQ MEHHANIVPW QMLCARVGAE LRVIPLNPDG
160 170 180 190 200
TLQLETLPTL FDEKTRLLAI THVSNVLGTE NPLAEMITLA HQHGAKVLVD
210 220 230 240 250
GAQAVMHHPV DVQALDCDFY VFSGHKLYGP TGIGILYVKE ALLQEMPPWE
260 270 280 290 300
GGGSMIATVS LSEGTTWTKA PWRFEAGTPN TGGIIGLGAA LEYVSALGLN
310 320 330 340 350
NIAEYEQNLM HYALSQLESV PDLTLYGPQN RLGVIAFNLG KHHAYDVGSF
360 370 380 390 400
LDNYGIAVRT GHHCAMPLMA YYNVPAMCRA SLAMYNTHEE VDRLVTGLQR

IHRLLG
Length:406
Mass (Da):44,434
Last modified:February 1, 1997 - v1
Checksum:i9374C43C3AD9D8E3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB055108 Genomic DNA Translation: BAB21542.1
U00096 Genomic DNA Translation: AAC74750.1
AP009048 Genomic DNA Translation: BAA15457.1
PIRiH64925
RefSeqiNP_416195.1, NC_000913.3
WP_000577988.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74750; AAC74750; b1680
BAA15457; BAA15457; BAA15457
GeneIDi946185
KEGGiecj:JW1670
eco:b1680
PATRICifig|1411691.4.peg.578

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB055108 Genomic DNA Translation: BAB21542.1
U00096 Genomic DNA Translation: AAC74750.1
AP009048 Genomic DNA Translation: BAA15457.1
PIRiH64925
RefSeqiNP_416195.1, NC_000913.3
WP_000577988.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1C0NX-ray2.80A1-406[»]
1I29X-ray2.80A1-406[»]
1JF9X-ray2.00A1-406[»]
1KMJX-ray2.00A1-406[»]
1KMKX-ray2.20A1-406[»]
5DB5X-ray2.75A/B1-406[»]
ProteinModelPortaliP77444
SMRiP77444
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260281, 100 interactors
ComplexPortaliCPX-2124 SufS complex
DIPiDIP-9324N
IntActiP77444, 14 interactors
STRINGi316385.ECDH10B_1814

Chemistry databases

DrugBankiDB04217 L-2-amino-3-butynoic acid
DB02761 S-Mercaptocysteine
DB03049 S-Selanyl Cysteine
DB02345 Selenocysteine

Proteomic databases

EPDiP77444
PaxDbiP77444
PRIDEiP77444

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74750; AAC74750; b1680
BAA15457; BAA15457; BAA15457
GeneIDi946185
KEGGiecj:JW1670
eco:b1680
PATRICifig|1411691.4.peg.578

Organism-specific databases

EchoBASEiEB3720
EcoGeneiEG13962 sufS

Phylogenomic databases

eggNOGiENOG4105C9B Bacteria
COG0520 LUCA
HOGENOMiHOG000017511
InParanoidiP77444
KOiK11717
PhylomeDBiP77444

Enzyme and pathway databases

UniPathwayi
UPA00266

BioCyciEcoCyc:G6906-MONOMER
MetaCyc:G6906-MONOMER
BRENDAi2.8.1.7 2026
4.4.1.16 2026

Miscellaneous databases

EvolutionaryTraceiP77444
PROiPR:P77444

Family and domain databases

Gene3Di3.40.640.10, 1 hit
3.90.1150.10, 1 hit
HAMAPiMF_01831 SufS_aminotrans_5, 1 hit
InterProiView protein in InterPro
IPR000192 Aminotrans_V_dom
IPR020578 Aminotrans_V_PyrdxlP_BS
IPR010970 Cys_dSase_SufS
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
PfamiView protein in Pfam
PF00266 Aminotran_5, 1 hit
SUPFAMiSSF53383 SSF53383, 1 hit
TIGRFAMsiTIGR01979 sufS, 1 hit
PROSITEiView protein in PROSITE
PS00595 AA_TRANSFER_CLASS_5, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSUFS_ECOLI
AccessioniPrimary (citable) accession number: P77444
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: February 1, 1997
Last modified: November 7, 2018
This is version 160 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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