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UniProtKB - P77399 (FADJ_ECOLI)
Protein
Fatty acid oxidation complex subunit alpha
Gene
fadJ
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the formation of a hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities. Strongly involved in the anaerobic degradation of long and medium-chain fatty acids in the presence of nitrate and weakly involved in the aerobic degradation of long-chain fatty acids.
2 PublicationsCatalytic activityi
: fatty acid beta-oxidation Pathwayi
This protein is involved in the pathway fatty acid beta-oxidation, which is part of Lipid metabolism.View all proteins of this organism that are known to be involved in the pathway fatty acid beta-oxidation and in Lipid metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 118 | Important for catalytic activityBy similarity | 1 | |
Sitei | 140 | Important for catalytic activityBy similarity | 1 |
GO - Molecular functioni
- 3-hydroxyacyl-CoA dehydrogenase activity Source: EcoCyc
- 3-hydroxybutyryl-CoA epimerase activity Source: UniProtKB-UniRule
- enoyl-CoA hydratase activity Source: EcoCyc
- long-chain-3-hydroxyacyl-CoA dehydrogenase activity Source: GO_Central
- NAD+ binding Source: InterPro
GO - Biological processi
- fatty acid beta-oxidation Source: ComplexPortal
Keywordsi
Molecular function | Isomerase, Lyase, Multifunctional enzyme, Oxidoreductase |
Biological process | Fatty acid metabolism, Lipid degradation, Lipid metabolism |
Ligand | NAD |
Enzyme and pathway databases
BioCyci | EcoCyc:G7212-MONOMER |
UniPathwayi | UPA00659 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:fadJ Synonyms:yfcX Ordered Locus Names:b2341, JW2338 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Other locations
- cytoplasm Source: UniProtKB-SubCell
- fatty acid beta-oxidation multienzyme complex Source: ComplexPortal
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000109298 | 1 – 714 | Fatty acid oxidation complex subunit alphaAdd BLAST | 714 |
Proteomic databases
jPOSTi | P77399 |
PaxDbi | P77399 |
PRIDEi | P77399 |
Expressioni
Inductioni
Unlike the aerobic pathway, the anaerobic pathway is not strongly repressed by FadR regulatory protein.
Interactioni
Subunit structurei
Heterotetramer of two alpha chains (FadJ) and two beta chains (FadI).
By similarityBinary interactionsi
P77399
With | #Exp. | IntAct |
---|---|---|
yfdH [P77293] | 2 | EBI-545361,EBI-545379 |
Protein-protein interaction databases
BioGRIDi | 4260533, 252 interactors |
ComplexPortali | CPX-3966, fadJI fatty acid oxidation complex, anaerobic conditions |
DIPi | DIP-11990N |
IntActi | P77399, 8 interactors |
STRINGi | 511145.b2341 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 190 | Enoyl-CoA hydrataseAdd BLAST | 190 | |
Regioni | 306 – 714 | 3-hydroxyacyl-CoA dehydrogenaseAdd BLAST | 409 |
Sequence similaritiesi
In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.Curated
In the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.Curated
Phylogenomic databases
eggNOGi | COG1024, Bacteria COG1250, Bacteria |
HOGENOMi | CLU_009834_16_1_6 |
InParanoidi | P77399 |
PhylomeDBi | P77399 |
Family and domain databases
HAMAPi | MF_01617, FadJ, 1 hit |
InterProi | View protein in InterPro IPR006180, 3-OHacyl-CoA_DH_CS IPR006176, 3-OHacyl-CoA_DH_NAD-bd IPR006108, 3HC_DH_C IPR008927, 6-PGluconate_DH-like_C_sf IPR029045, ClpP/crotonase-like_dom_sf IPR001753, Enoyl-CoA_hydra/iso IPR012802, FadJ IPR036291, NAD(P)-bd_dom_sf |
Pfami | View protein in Pfam PF00725, 3HCDH, 2 hits PF02737, 3HCDH_N, 1 hit PF00378, ECH_1, 1 hit |
SUPFAMi | SSF48179, SSF48179, 2 hits SSF51735, SSF51735, 1 hit SSF52096, SSF52096, 1 hit |
TIGRFAMsi | TIGR02440, FadJ, 1 hit |
PROSITEi | View protein in PROSITE PS00067, 3HCDH, 1 hit |
i Sequence
Sequence statusi: Complete.
P77399-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEMTSAFTLN VRLDNIAVIT IDVPGEKMNT LKAEFASQVR AIIKQLRENK
60 70 80 90 100
ELRGVVFVSA KPDNFIAGAD INMIGNCKTA QEAEALARQG QQLMAEIHAL
110 120 130 140 150
PIQVIAAIHG ACLGGGLELA LACHGRVCTD DPKTVLGLPE VQLGLLPGSG
160 170 180 190 200
GTQRLPRLIG VSTALEMILT GKQLRAKQAL KLGLVDDVVP HSILLEAAVE
210 220 230 240 250
LAKKERPSSR PLPVRERILA GPLGRALLFK MVGKKTEHKT QGNYPATERI
260 270 280 290 300
LEVVETGLAQ GTSSGYDAEA RAFGELAMTP QSQALRSIFF ASTDVKKDPG
310 320 330 340 350
SDAPPAPLNS VGILGGGLMG GGIAYVTACK AGIPVRIKDI NPQGINHALK
360 370 380 390 400
YSWDQLEGKV RRRHLKASER DKQLALISGT TDYRGFAHRD LIIEAVFENL
410 420 430 440 450
ELKQQMVAEV EQNCAAHTIF ASNTSSLPIG DIAAHATRPE QVIGLHFFSP
460 470 480 490 500
VEKMPLVEII PHAGTSAQTI ATTVKLAKKQ GKTPIVVRDK AGFYVNRILA
510 520 530 540 550
PYINEAIRML TQGERVEHID AALVKFGFPV GPIQLLDEVG IDTGTKIIPV
560 570 580 590 600
LEAAYGERFS APANVVSSIL NDDRKGRKNG RGFYLYGQKG RKSKKQVDPA
610 620 630 640 650
IYPLIGTQGQ GRISAPQVAE RCVMLMLNEA VRCVDEQVIR SVRDGDIGAV
660 670 680 690 700
FGIGFPPFLG GPFRYIDSLG AGEVVAIMQR LATQYGSRFT PCERLVEMGA
710
RGESFWKTTA TDLQ
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC75401.1 AP009048 Genomic DNA Translation: BAA16195.1 |
PIRi | C65007 |
RefSeqi | NP_416843.1, NC_000913.3 WP_000426176.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC75401; AAC75401; b2341 BAA16195; BAA16195; BAA16195 |
GeneIDi | 949097 |
KEGGi | ecj:JW2338 eco:b2341 |
PATRICi | fig|1411691.4.peg.4391 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC75401.1 AP009048 Genomic DNA Translation: BAA16195.1 |
PIRi | C65007 |
RefSeqi | NP_416843.1, NC_000913.3 WP_000426176.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6YSV | X-ray | 2.70 | A/B | 1-714 | [»] | |
6YSW | X-ray | 2.82 | A/B | 1-714 | [»] | |
SASBDBi | P77399 | |||||
SMRi | P77399 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260533, 252 interactors |
ComplexPortali | CPX-3966, fadJI fatty acid oxidation complex, anaerobic conditions |
DIPi | DIP-11990N |
IntActi | P77399, 8 interactors |
STRINGi | 511145.b2341 |
Proteomic databases
jPOSTi | P77399 |
PaxDbi | P77399 |
PRIDEi | P77399 |
Genome annotation databases
EnsemblBacteriai | AAC75401; AAC75401; b2341 BAA16195; BAA16195; BAA16195 |
GeneIDi | 949097 |
KEGGi | ecj:JW2338 eco:b2341 |
PATRICi | fig|1411691.4.peg.4391 |
Organism-specific databases
EchoBASEi | EB3879 |
Phylogenomic databases
eggNOGi | COG1024, Bacteria COG1250, Bacteria |
HOGENOMi | CLU_009834_16_1_6 |
InParanoidi | P77399 |
PhylomeDBi | P77399 |
Enzyme and pathway databases
UniPathwayi | UPA00659 |
BioCyci | EcoCyc:G7212-MONOMER |
Miscellaneous databases
PROi | PR:P77399 |
Family and domain databases
HAMAPi | MF_01617, FadJ, 1 hit |
InterProi | View protein in InterPro IPR006180, 3-OHacyl-CoA_DH_CS IPR006176, 3-OHacyl-CoA_DH_NAD-bd IPR006108, 3HC_DH_C IPR008927, 6-PGluconate_DH-like_C_sf IPR029045, ClpP/crotonase-like_dom_sf IPR001753, Enoyl-CoA_hydra/iso IPR012802, FadJ IPR036291, NAD(P)-bd_dom_sf |
Pfami | View protein in Pfam PF00725, 3HCDH, 2 hits PF02737, 3HCDH_N, 1 hit PF00378, ECH_1, 1 hit |
SUPFAMi | SSF48179, SSF48179, 2 hits SSF51735, SSF51735, 1 hit SSF52096, SSF52096, 1 hit |
TIGRFAMsi | TIGR02440, FadJ, 1 hit |
PROSITEi | View protein in PROSITE PS00067, 3HCDH, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | FADJ_ECOLI | |
Accessioni | P77399Primary (citable) accession number: P77399 Secondary accession number(s): Q6KCX2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | February 1, 1997 | |
Last modified: | February 23, 2022 | |
This is version 178 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families