UniProtKB - P77397 (MHPA_ECOLI)
Protein
3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid hydroxylase
Gene
mhpA
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the insertion of one atom of molecular oxygen into position 2 of the phenyl ring of 3-(3-hydroxyphenyl)propionate (3-HPP) and hydroxycinnamic acid (3HCI).1 Publication
Catalytic activityi
- EC:1.14.13.127
- (2E)-3-(3-hydroxyphenyl)prop-2-enoate + H+ + NADH + O2 = (2E)-3-(2,3-dihydroxyphenyl)prop-2-enoate + H2O + NAD+EC:1.14.13.127
Cofactori
FADBy similarity
: 3-phenylpropanoate degradation Pathwayi
This protein is involved in the pathway 3-phenylpropanoate degradation, which is part of Aromatic compound metabolism.View all proteins of this organism that are known to be involved in the pathway 3-phenylpropanoate degradation and in Aromatic compound metabolism.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 17 – 46 | FADSequence analysisAdd BLAST | 30 | |
Nucleotide bindingi | 285 – 295 | FADSequence analysisAdd BLAST | 11 |
GO - Molecular functioni
- 3-(3-hydroxyphenyl)propionate hydroxylase activity Source: GO_Central
- FAD binding Source: InterPro
GO - Biological processi
- 3-(3-hydroxy)phenylpropionate catabolic process Source: EcoCyc
- 3-phenylpropionate catabolic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Aromatic hydrocarbons catabolism |
Ligand | FAD, Flavoprotein, NAD |
Enzyme and pathway databases
BioCyci | EcoCyc:MHPHYDROXY-MONOMER ECOL316407:JW0338-MONOMER MetaCyc:MHPHYDROXY-MONOMER |
UniPathwayi | UPA00714 |
Names & Taxonomyi
Protein namesi | Recommended name: 3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid hydroxylase (EC:1.14.13.127)Short name: 3-HCI hydroxylase Short name: 3-HPP hydroxylase |
Gene namesi | Name:mhpA Ordered Locus Names:b0347, JW0338 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000214043 | 1 – 554 | 3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid hydroxylaseAdd BLAST | 554 |
Proteomic databases
jPOSTi | P77397 |
PaxDbi | P77397 |
PRIDEi | P77397 |
Interactioni
Protein-protein interaction databases
BioGridi | 4260728, 15 interactors 849583, 1 interactor |
IntActi | P77397, 3 interactors |
STRINGi | 511145.b0347 |
Family & Domainsi
Sequence similaritiesi
Belongs to the PheA/TfdB FAD monooxygenase family.Curated
Phylogenomic databases
eggNOGi | ENOG4105E2Q Bacteria COG0654 LUCA |
HOGENOMi | HOG000221401 |
InParanoidi | P77397 |
KOi | K05712 |
PhylomeDBi | P77397 |
Family and domain databases
Gene3Di | 3.50.50.60, 1 hit |
HAMAPi | MF_01652 MhpA, 1 hit |
InterProi | View protein in InterPro IPR023786 3-HPP/3HCI_hydroxylase IPR002938 FAD-bd IPR036188 FAD/NAD-bd_sf |
Pfami | View protein in Pfam PF01494 FAD_binding_3, 1 hit |
SUPFAMi | SSF51905 SSF51905, 1 hit |
i Sequence
Sequence statusi: Complete.
P77397-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAIQHPDIQP AVNHSVQVAI AGAGPVGLMM ANYLGQMGID VLVVEKLDKL
60 70 80 90 100
IDYPRAIGID DEALRTMQSV GLVDDVLPHT TPWHAMRFLT PKGRCFADIQ
110 120 130 140 150
PMTDEFGWPR RNAFIQPQVD AVMLEGVSRF PNVRCLFSRE LEAFSQQDDE
160 170 180 190 200
VTLHLKTAEG QREIVKAQWL VACDGGASFV RRTLNVPFEG KTAPNQWIVV
210 220 230 240 250
DIANDPLSTP HIYLCCDPVR PYVSAALPHA VRRFEFMVMP GETEEQLREP
260 270 280 290 300
QNMRKLLSKV LPNPDNVELI RQRVYTHNAR LAQRFRIDRV LLAGDAAHIM
310 320 330 340 350
PVWQGQGYNS GMRDAFNLAW KLALVIQGKA RDALLDTYQQ ERRDHAKAMI
360 370 380 390 400
DLSVTAGNVL APPKRWQGTL RDGVSWLLNY LPPVKRYFLE MRFKPMPQYY
410 420 430 440 450
GGALMREGEA KHSPVGKMFI QPKVTLENGD VTLLDNAIGA NFAVIGWGCN
460 470 480 490 500
PLWGMSDEQI QQWRALGTRF IQVVPEVQIH TAQDNHDGVL RVGDTQGRLR
510 520 530 540 550
SWFAQHNASL VVMRPDRFVA ATAIPQTLGK TLNKLASVMT LTRPDADVSV
EKVA
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 272 | Q → H in BAA13052 (Ref. 1) Curated | 1 | |
Sequence conflicti | 360 | L → P in BAA13052 (Ref. 1) Curated | 1 | |
Sequence conflicti | 502 | W → G in BAA13052 (Ref. 1) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D86239 Genomic DNA Translation: BAA13052.1 Y09555 Genomic DNA Translation: CAA70747.1 U73857 Genomic DNA Translation: AAB18071.1 U00096 Genomic DNA Translation: AAC73450.1 AP009048 Genomic DNA Translation: BAE76129.1 |
PIRi | C64762 |
RefSeqi | NP_414881.1, NC_000913.3 WP_001007407.1, NZ_SSZK01000061.1 |
Genome annotation databases
EnsemblBacteriai | AAC73450; AAC73450; b0347 BAE76129; BAE76129; BAE76129 |
GeneIDi | 945197 |
KEGGi | ecj:JW0338 eco:b0347 |
PATRICi | fig|1411691.4.peg.1931 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D86239 Genomic DNA Translation: BAA13052.1 Y09555 Genomic DNA Translation: CAA70747.1 U73857 Genomic DNA Translation: AAB18071.1 U00096 Genomic DNA Translation: AAC73450.1 AP009048 Genomic DNA Translation: BAE76129.1 |
PIRi | C64762 |
RefSeqi | NP_414881.1, NC_000913.3 WP_001007407.1, NZ_SSZK01000061.1 |
3D structure databases
SMRi | P77397 |
ModBasei | Search... |
Protein-protein interaction databases
BioGridi | 4260728, 15 interactors 849583, 1 interactor |
IntActi | P77397, 3 interactors |
STRINGi | 511145.b0347 |
Proteomic databases
jPOSTi | P77397 |
PaxDbi | P77397 |
PRIDEi | P77397 |
Genome annotation databases
EnsemblBacteriai | AAC73450; AAC73450; b0347 BAE76129; BAE76129; BAE76129 |
GeneIDi | 945197 |
KEGGi | ecj:JW0338 eco:b0347 |
PATRICi | fig|1411691.4.peg.1931 |
Organism-specific databases
EchoBASEi | EB4166 |
Phylogenomic databases
eggNOGi | ENOG4105E2Q Bacteria COG0654 LUCA |
HOGENOMi | HOG000221401 |
InParanoidi | P77397 |
KOi | K05712 |
PhylomeDBi | P77397 |
Enzyme and pathway databases
UniPathwayi | UPA00714 |
BioCyci | EcoCyc:MHPHYDROXY-MONOMER ECOL316407:JW0338-MONOMER MetaCyc:MHPHYDROXY-MONOMER |
Miscellaneous databases
PROi | PR:P77397 |
Family and domain databases
Gene3Di | 3.50.50.60, 1 hit |
HAMAPi | MF_01652 MhpA, 1 hit |
InterProi | View protein in InterPro IPR023786 3-HPP/3HCI_hydroxylase IPR002938 FAD-bd IPR036188 FAD/NAD-bd_sf |
Pfami | View protein in Pfam PF01494 FAD_binding_3, 1 hit |
SUPFAMi | SSF51905 SSF51905, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MHPA_ECOLI | |
Accessioni | P77397Primary (citable) accession number: P77397 Secondary accession number(s): P71203, P77047, Q2MC77 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | February 1, 1997 | |
Last modified: | November 13, 2019 | |
This is version 145 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene - PATHWAY comments
Index of metabolic and biosynthesis pathways