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UniProtKB - P77397 (MHPA_ECOLI)

Entry version 145 (13 Nov 2019)
Sequence version 1 (01 Feb 1997)
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Protein

3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid hydroxylase

Gene

mhpA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the insertion of one atom of molecular oxygen into position 2 of the phenyl ring of 3-(3-hydroxyphenyl)propionate (3-HPP) and hydroxycinnamic acid (3HCI).1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the ‘Function’ section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

FADBy similarity

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: 3-phenylpropanoate degradation

This protein is involved in the pathway 3-phenylpropanoate degradation, which is part of Aromatic compound metabolism.
View all proteins of this organism that are known to be involved in the pathway 3-phenylpropanoate degradation and in Aromatic compound metabolism.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi17 – 46FADSequence analysisAdd BLAST30
Nucleotide bindingi285 – 295FADSequence analysisAdd BLAST11

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionOxidoreductase
Biological processAromatic hydrocarbons catabolism
LigandFAD, Flavoprotein, NAD

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...BioCyci		EcoCyc:MHPHYDROXY-MONOMER
ECOL316407:JW0338-MONOMER
MetaCyc:MHPHYDROXY-MONOMER

UniPathway: a resource for the exploration and annotation of metabolic pathways

More...UniPathwayi		UPA00714

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid hydroxylase (EC:1.14.13.127)
Short name:
3-HCI hydroxylase
Short name:
3-HPP hydroxylase
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:mhpA
Ordered Locus Names:b0347, JW0338
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia ›
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00002140431 – 5543-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid hydroxylaseAdd BLAST554

Proteomic databases

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		P77397

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		P77397

PRoteomics IDEntifications database

More...PRIDEi		P77397

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

More...BioGridi		4260728, 15 interactors
849583, 1 interactor

Protein interaction database and analysis system

More...IntActi		P77397, 3 interactors

STRING: functional protein association networks

More...STRINGi		511145.b0347

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		P77397

Database of comparative protein structure models

More...ModBasei		Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the PheA/TfdB FAD monooxygenase family.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		ENOG4105E2Q Bacteria
COG0654 LUCA

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		HOG000221401

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		P77397

KEGG Orthology (KO)

More...KOi		K05712

Database for complete collections of gene phylogenies

More...PhylomeDBi		P77397

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		3.50.50.60, 1 hit

HAMAP database of protein families

More...HAMAPi		MF_01652 MhpA, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR023786 3-HPP/3HCI_hydroxylase
IPR002938 FAD-bd
IPR036188 FAD/NAD-bd_sf

Pfam protein domain database

More...Pfami		View protein in Pfam
PF01494 FAD_binding_3, 1 hit

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF51905 SSF51905, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P77397-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAIQHPDIQP AVNHSVQVAI AGAGPVGLMM ANYLGQMGID VLVVEKLDKL 
        60         70         80         90        100
IDYPRAIGID DEALRTMQSV GLVDDVLPHT TPWHAMRFLT PKGRCFADIQ 
       110        120        130        140        150
PMTDEFGWPR RNAFIQPQVD AVMLEGVSRF PNVRCLFSRE LEAFSQQDDE 
       160        170        180        190        200
VTLHLKTAEG QREIVKAQWL VACDGGASFV RRTLNVPFEG KTAPNQWIVV 
       210        220        230        240        250
DIANDPLSTP HIYLCCDPVR PYVSAALPHA VRRFEFMVMP GETEEQLREP 
       260        270        280        290        300
QNMRKLLSKV LPNPDNVELI RQRVYTHNAR LAQRFRIDRV LLAGDAAHIM 
       310        320        330        340        350
PVWQGQGYNS GMRDAFNLAW KLALVIQGKA RDALLDTYQQ ERRDHAKAMI 
       360        370        380        390        400
DLSVTAGNVL APPKRWQGTL RDGVSWLLNY LPPVKRYFLE MRFKPMPQYY 
       410        420        430        440        450
GGALMREGEA KHSPVGKMFI QPKVTLENGD VTLLDNAIGA NFAVIGWGCN 
       460        470        480        490        500
PLWGMSDEQI QQWRALGTRF IQVVPEVQIH TAQDNHDGVL RVGDTQGRLR 
       510        520        530        540        550
SWFAQHNASL VVMRPDRFVA ATAIPQTLGK TLNKLASVMT LTRPDADVSV 

EKVA
Length:554
Mass (Da):62,186
Last modified:February 1, 1997 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i1D56CB799E9F8A8E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti272Q → H in BAA13052 (Ref. 1) Curated1
Sequence conflicti360L → P in BAA13052 (Ref. 1) Curated1
Sequence conflicti502W → G in BAA13052 (Ref. 1) Curated1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
D86239 Genomic DNA Translation: BAA13052.1
Y09555 Genomic DNA Translation: CAA70747.1
U73857 Genomic DNA Translation: AAB18071.1
U00096 Genomic DNA Translation: AAC73450.1
AP009048 Genomic DNA Translation: BAE76129.1

Protein sequence database of the Protein Information Resource

More...PIRi		C64762

NCBI Reference Sequences

More...RefSeqi		NP_414881.1, NC_000913.3
WP_001007407.1, NZ_SSZK01000061.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...EnsemblBacteriai		AAC73450; AAC73450; b0347
BAE76129; BAE76129; BAE76129

Database of genes from NCBI RefSeq genomes

More...GeneIDi		945197

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		ecj:JW0338
eco:b0347

Pathosystems Resource Integration Center (PATRIC)

More...PATRICi		fig|1411691.4.peg.1931

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D86239 Genomic DNA Translation: BAA13052.1
Y09555 Genomic DNA Translation: CAA70747.1
U73857 Genomic DNA Translation: AAB18071.1
U00096 Genomic DNA Translation: AAC73450.1
AP009048 Genomic DNA Translation: BAE76129.1
PIRiC64762
RefSeqiNP_414881.1, NC_000913.3
WP_001007407.1, NZ_SSZK01000061.1

3D structure databases

SMRiP77397
ModBaseiSearch...

Protein-protein interaction databases

BioGridi4260728, 15 interactors
849583, 1 interactor
IntActiP77397, 3 interactors
STRINGi511145.b0347

Proteomic databases

jPOSTiP77397
PaxDbiP77397
PRIDEiP77397

Genome annotation databases

EnsemblBacteriaiAAC73450; AAC73450; b0347
BAE76129; BAE76129; BAE76129
GeneIDi945197
KEGGiecj:JW0338
eco:b0347
PATRICifig|1411691.4.peg.1931

Organism-specific databases

EchoBASE - an integrated post-genomic database for E. coli

More...EchoBASEi		EB4166

Phylogenomic databases

eggNOGiENOG4105E2Q Bacteria
COG0654 LUCA
HOGENOMiHOG000221401
InParanoidiP77397
KOiK05712
PhylomeDBiP77397

Enzyme and pathway databases

UniPathwayiUPA00714
BioCyciEcoCyc:MHPHYDROXY-MONOMER
ECOL316407:JW0338-MONOMER
MetaCyc:MHPHYDROXY-MONOMER

Miscellaneous databases

Protein Ontology

More...PROi		PR:P77397

Family and domain databases

Gene3Di3.50.50.60, 1 hit
HAMAPiMF_01652 MhpA, 1 hit
InterProiView protein in InterPro
IPR023786 3-HPP/3HCI_hydroxylase
IPR002938 FAD-bd
IPR036188 FAD/NAD-bd_sf
PfamiView protein in Pfam
PF01494 FAD_binding_3, 1 hit
SUPFAMiSSF51905 SSF51905, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...ProtoNeti		Search...

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiMHPA_ECOLI
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P77397
Secondary accession number(s): P71203, P77047, Q2MC77
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: November 13, 2019
This is version 145 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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