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UniProtKB - P77364 (GLXK2_ECOLI)
Protein
Glycerate 3-kinase
Gene
glxK
Organism
Escherichia coli (strain K12)
Status
Functioni
Miscellaneous
GK1 has a half-life of 92 minutes while GK2 has a half-life of 11 minutes at 49 degrees Celsius.
Caution
E.coli has 2 glycerate kinases, GK1 and GK2; it is not clear which gene encodes which enzyme. PubMed:5325263 may be a mix of GK1 and GK2.Curated
Catalytic activityi
- EC:2.7.1.31
Cofactori
Mg2+1 Publication, Co2+1 Publication, Mn2+1 Publication, Fe2+1 Publication, Ca2+1 PublicationNote: Divalent metal cations; Mg2+ and Co2+ are better than Mn2+, Fe2+ or Ca2+.1 Publication
Activity regulationi
Inhibited by EDTA, p-hydroxy-mercuribenzoate and iodoacetate but not by NaF.1 Publication
Kineticsi
- KM=0.07 mM for glycerate1 Publication
pH dependencei
Optimum pH is 7.0-7.6.1 Publication
: glycolate degradation Pathwayi
This protein is involved in step 4 of the subpathway that synthesizes 3-phospho-D-glycerate from glycolate. This subpathway is part of the pathway glycolate degradation, which is itself part of Organic acid metabolism.View all proteins of this organism that are known to be involved in the subpathway that synthesizes 3-phospho-D-glycerate from glycolate, the pathway glycolate degradation and in Organic acid metabolism.
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- glycerate 2-kinase activity Source: EcoCyc
- glycerate kinase activity Source: UniProtKB-EC
GO - Biological processi
- glycolate catabolic process Source: EcoCyc
- glyoxylate catabolic process Source: EcoCyc
- organic acid phosphorylation Source: InterPro
Keywordsi
Molecular function | Kinase, Transferase |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:GLY3KIN-MONOMER |
BRENDAi | 2.7.1.165, 2165 |
UniPathwayi | UPA00864;UER00833 |
Names & Taxonomyi
Protein namesi | Recommended name: Glycerate 3-kinase (EC:2.7.1.31)Alternative name(s): D-Glycerate-3-kinase Glycerate kinase 2 Short name: GK2 |
Gene namesi | Name:glxK Synonyms:glxB5, ybbZ Ordered Locus Names:b0514, JW0502 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Loss of glycerate kinase activity.1 Publication
Chemistry databases
ChEMBLi | CHEMBL3309014 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000071531 | 1 – 381 | Glycerate 3-kinaseAdd BLAST | 381 |
Proteomic databases
jPOSTi | P77364 |
PaxDbi | P77364 |
PRIDEi | P77364 |
Expressioni
Inductioni
In glycolate-grown cells but not in glucose-grown cells (at protein level).2 Publications
Interactioni
Protein-protein interaction databases
BioGRIDi | 4262012, 12 interactors |
IntActi | P77364, 3 interactors |
STRINGi | 511145.b0514 |
Family & Domainsi
Sequence similaritiesi
Belongs to the glycerate kinase type-1 family.Curated
Phylogenomic databases
eggNOGi | COG1929, Bacteria |
HOGENOMi | CLU_028255_0_1_6 |
InParanoidi | P77364 |
PhylomeDBi | P77364 |
Family and domain databases
Gene3Di | 3.40.50.10350, 1 hit 3.90.1510.10, 1 hit |
InterProi | View protein in InterPro IPR018193, Glyc_kinase_flavodox-like_fold IPR004381, Glycerate_kinase IPR018197, Glycerate_kinase_RE-like IPR036129, Glycerate_kinase_sf |
PANTHERi | PTHR21599, PTHR21599, 1 hit |
Pfami | View protein in Pfam PF02595, Gly_kinase, 1 hit |
PIRSFi | PIRSF006078, GlxK, 1 hit |
SUPFAMi | SSF110738, SSF110738, 1 hit |
TIGRFAMsi | TIGR00045, TIGR00045, 1 hit |
i Sequence
Sequence statusi: Complete.
P77364-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKIVIAPDSF KESLSAEKCC QAIKAGFSTL FPDANYICLP IADGGEGTVD
60 70 80 90 100
AMVAATGGNI VTLEVCGPMG EKVNAFYGLT GDGKTAVIEM AAASGLMLVA
110 120 130 140 150
PEKRNPLLAS SFGTGELIRH ALDNDIRHII LGIGGSATVD GGMGMAQALG
160 170 180 190 200
VRFLDADGQA LAANGGNLAR VASIEMDECD PRLANCHIEV ACDVDNPLVG
210 220 230 240 250
ARGAAAVFGP QKGATPEMVE ELEQGLQNYA RVLQQQTEIN VCQMAGGGAA
260 270 280 290 300
GGMGIAAAVF LNADIKPGIE IVLNAVNLAQ AVQGAALVIT GEGRIDSQTA
310 320 330 340 350
GGKAPLGVAS VAKQFNVPVI GIAGVLGDGV EVVHQYGIDA VFSILPRLAP
360 370 380
LAEVLASGET NLFNSARNIA CAIKIGQGIK N
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U89279 Genomic DNA Translation: AAB93855.1 U82664 Genomic DNA Translation: AAB40266.1 U00096 Genomic DNA Translation: AAC73616.1 AP009048 Genomic DNA Translation: BAE76292.1 |
PIRi | A64783 |
RefSeqi | NP_415047.1, NC_000913.3 WP_001333621.1, NZ_SSZK01000024.1 |
Genome annotation databases
EnsemblBacteriai | AAC73616; AAC73616; b0514 BAE76292; BAE76292; BAE76292 |
GeneIDi | 945129 |
KEGGi | ecj:JW0502 eco:b0514 |
PATRICi | fig|1411691.4.peg.1764 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U89279 Genomic DNA Translation: AAB93855.1 U82664 Genomic DNA Translation: AAB40266.1 U00096 Genomic DNA Translation: AAC73616.1 AP009048 Genomic DNA Translation: BAE76292.1 |
PIRi | A64783 |
RefSeqi | NP_415047.1, NC_000913.3 WP_001333621.1, NZ_SSZK01000024.1 |
3D structure databases
SMRi | P77364 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4262012, 12 interactors |
IntActi | P77364, 3 interactors |
STRINGi | 511145.b0514 |
Chemistry databases
ChEMBLi | CHEMBL3309014 |
Proteomic databases
jPOSTi | P77364 |
PaxDbi | P77364 |
PRIDEi | P77364 |
Genome annotation databases
EnsemblBacteriai | AAC73616; AAC73616; b0514 BAE76292; BAE76292; BAE76292 |
GeneIDi | 945129 |
KEGGi | ecj:JW0502 eco:b0514 |
PATRICi | fig|1411691.4.peg.1764 |
Organism-specific databases
EchoBASEi | EB3386 |
Phylogenomic databases
eggNOGi | COG1929, Bacteria |
HOGENOMi | CLU_028255_0_1_6 |
InParanoidi | P77364 |
PhylomeDBi | P77364 |
Enzyme and pathway databases
UniPathwayi | UPA00864;UER00833 |
BioCyci | EcoCyc:GLY3KIN-MONOMER |
BRENDAi | 2.7.1.165, 2165 |
Miscellaneous databases
PROi | PR:P77364 |
Family and domain databases
Gene3Di | 3.40.50.10350, 1 hit 3.90.1510.10, 1 hit |
InterProi | View protein in InterPro IPR018193, Glyc_kinase_flavodox-like_fold IPR004381, Glycerate_kinase IPR018197, Glycerate_kinase_RE-like IPR036129, Glycerate_kinase_sf |
PANTHERi | PTHR21599, PTHR21599, 1 hit |
Pfami | View protein in Pfam PF02595, Gly_kinase, 1 hit |
PIRSFi | PIRSF006078, GlxK, 1 hit |
SUPFAMi | SSF110738, SSF110738, 1 hit |
TIGRFAMsi | TIGR00045, TIGR00045, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GLXK2_ECOLI | |
Accessioni | P77364Primary (citable) accession number: P77364 Secondary accession number(s): Q2MBR4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | February 1, 1997 | |
Last modified: | February 23, 2022 | |
This is version 146 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Uncharacterized protein families (UPF)
List of uncharacterized protein family (UPF) entries - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families