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UniProtKB - P77364 (GLXK2_ECOLI)

Entry version 143 (12 Aug 2020)
Sequence version 1 (01 Feb 1997)
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Protein

Glycerate 3-kinase

Gene

glxK

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

GK1 has a half-life of 92 minutes while GK2 has a half-life of 11 minutes at 49 degrees Celsius.

Caution

E.coli has 2 glycerate kinases, GK1 and GK2; it is not clear which gene encodes which enzyme. PubMed:5325263 may be a mix of GK1 and GK2.Curated

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Mg2+1 Publication, Co2+1 Publication, Mn2+1 Publication, Fe2+1 Publication, Ca2+1 PublicationNote: Divalent metal cations; Mg2+ and Co2+ are better than Mn2+, Fe2+ or Ca2+.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

Inhibited by EDTA, p-hydroxy-mercuribenzoate and iodoacetate but not by NaF.1 Publication

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

  1. KM=0.07 mM for glycerate1 Publication

    pH dependencei

    Optimum pH is 7.0-7.6.1 Publication

    <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: glycolate degradation

    This protein is involved in step 4 of the subpathway that synthesizes 3-phospho-D-glycerate from glycolate.
    Proteins known to be involved in the 4 steps of the subpathway in this organism are:
    1. no protein annotated in this organism
    2. Glyoxylate carboligase (gcl)
    3. 2-hydroxy-3-oxopropionate reductase (glxR)
    4. Glycerate 3-kinase (glxK)
    This subpathway is part of the pathway glycolate degradation, which is itself part of Organic acid metabolism.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes 3-phospho-D-glycerate from glycolate, the pathway glycolate degradation and in Organic acid metabolism.

    <p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

    Complete GO annotation on QuickGO ...

    GO - Biological processi

    • glycolate catabolic process Source: EcoCyc
    • glyoxylate catabolic process Source: EcoCyc
    • organic acid phosphorylation Source: InterPro
    Complete GO annotation on QuickGO ...

    <p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

    Molecular functionKinase, Transferase
    LigandATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyc Collection of Pathway/Genome Databases

    More...    BioCyci    		EcoCyc:GLY3KIN-MONOMER
    ECOL316407:JW0502-MONOMER
    MetaCyc:GLY3KIN-MONOMER

    BRENDA Comprehensive Enzyme Information System

    More...    BRENDAi    		2.7.1.165, 2165

    UniPathway: a resource for the exploration and annotation of metabolic pathways

    More...    UniPathwayi    		UPA00864;UER00833

    <p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
    Recommended name:
    Glycerate 3-kinase (EC:2.7.1.31)
    Alternative name(s):
    D-Glycerate-3-kinase
    Glycerate kinase 2
    Short name:
    GK2
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
    Name:glxK
    Synonyms:glxB5, ybbZ
    Ordered Locus Names:b0514, JW0502
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia ›
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
    • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    <p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

    <p>This subsection of the 'Pathology and Biotech' section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

    Loss of glycerate kinase activity.1 Publication

    Chemistry databases

    ChEMBL database of bioactive drug-like small molecules

    More...    ChEMBLi    		CHEMBL3309014

    <p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000715311 – 381Glycerate 3-kinaseAdd BLAST381

    Proteomic databases

    jPOST - Japan Proteome Standard Repository/Database

    More...    jPOSTi    		P77364

    PaxDb, a database of protein abundance averages across all three domains of life

    More...    PaxDbi    		P77364

    PRoteomics IDEntifications database

    More...    PRIDEi    		P77364

    <p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

    <p>This subsection of the 'Expression' section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductioni

    In glycolate-grown cells but not in glucose-grown cells (at protein level).2 Publications

    <p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

    Protein-protein interaction databases

    The Biological General Repository for Interaction Datasets (BioGRID)

    More...    BioGRIDi    		4262012, 12 interactors

    Protein interaction database and analysis system

    More...    IntActi    		P77364, 3 interactors

    STRING: functional protein association networks

    More...    STRINGi    		511145.b0514

    <p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

    3D structure databases

    SWISS-MODEL Repository - a database of annotated 3D protein structure models

    More...    SMRi    		P77364

    Database of comparative protein structure models

    More...    ModBasei    		Search...

    <p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

    <p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

    Belongs to the glycerate kinase type-1 family.Curated

    Phylogenomic databases

    evolutionary genealogy of genes: Non-supervised Orthologous Groups

    More...    eggNOGi    		COG1929, Bacteria

    The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

    More...    HOGENOMi    		CLU_028255_0_1_6

    InParanoid: Eukaryotic Ortholog Groups

    More...    InParanoidi    		P77364

    KEGG Orthology (KO)

    More...    KOi    		K00865

    Database for complete collections of gene phylogenies

    More...    PhylomeDBi    		P77364

    Family and domain databases

    Gene3D Structural and Functional Annotation of Protein Families

    More...    Gene3Di    		3.40.50.10350, 1 hit
    3.90.1510.10, 1 hit

    Integrated resource of protein families, domains and functional sites

    More...    InterProi    		View protein in InterPro
    IPR018193, Glyc_kinase_flavodox-like_fold
    IPR004381, Glycerate_kinase
    IPR018197, Glycerate_kinase_RE-like
    IPR036129, Glycerate_kinase_sf

    The PANTHER Classification System

    More...    PANTHERi    		PTHR21599, PTHR21599, 1 hit

    Pfam protein domain database

    More...    Pfami    		View protein in Pfam
    PF02595, Gly_kinase, 1 hit

    PIRSF; a whole-protein classification database

    More...    PIRSFi    		PIRSF006078, GlxK, 1 hit

    Superfamily database of structural and functional annotation

    More...    SUPFAMi    		SSF110738, SSF110738, 1 hit

    TIGRFAMs; a protein family database

    More...    TIGRFAMsi    		TIGR00045, TIGR00045, 1 hit

    <p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

    <p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

    P77364-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MKIVIAPDSF KESLSAEKCC QAIKAGFSTL FPDANYICLP IADGGEGTVD 
            60         70         80         90        100
    AMVAATGGNI VTLEVCGPMG EKVNAFYGLT GDGKTAVIEM AAASGLMLVA 
           110        120        130        140        150
    PEKRNPLLAS SFGTGELIRH ALDNDIRHII LGIGGSATVD GGMGMAQALG 
           160        170        180        190        200
    VRFLDADGQA LAANGGNLAR VASIEMDECD PRLANCHIEV ACDVDNPLVG 
           210        220        230        240        250
    ARGAAAVFGP QKGATPEMVE ELEQGLQNYA RVLQQQTEIN VCQMAGGGAA 
           260        270        280        290        300
    GGMGIAAAVF LNADIKPGIE IVLNAVNLAQ AVQGAALVIT GEGRIDSQTA 
           310        320        330        340        350
    GGKAPLGVAS VAKQFNVPVI GIAGVLGDGV EVVHQYGIDA VFSILPRLAP 
           360        370        380 
    LAEVLASGET NLFNSARNIA CAIKIGQGIK N
    Length:381
    Mass (Da):38,734
    Last modified:February 1, 1997 - v1
    <p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i0F902DF27DE63578
    GO

    Sequence databases

    Select the link destinations:

    EMBL nucleotide sequence database

    More...    EMBLi

    GenBank nucleotide sequence database

    More...    GenBanki

    DNA Data Bank of Japan; a nucleotide sequence database

    More...    DDBJi
    Links Updated
    		U89279 Genomic DNA Translation: AAB93855.1
    U82664 Genomic DNA Translation: AAB40266.1
    U00096 Genomic DNA Translation: AAC73616.1
    AP009048 Genomic DNA Translation: BAE76292.1

    Protein sequence database of the Protein Information Resource

    More...    PIRi    		A64783

    NCBI Reference Sequences

    More...    RefSeqi    		NP_415047.1, NC_000913.3
    WP_001333621.1, NZ_SSZK01000024.1

    Genome annotation databases

    Ensembl bacterial and archaeal genome annotation project

    More...    EnsemblBacteriai    		AAC73616; AAC73616; b0514
    BAE76292; BAE76292; BAE76292

    Database of genes from NCBI RefSeq genomes

    More...    GeneIDi    		945129

    KEGG: Kyoto Encyclopedia of Genes and Genomes

    More...    KEGGi    		ecj:JW0502
    eco:b0514

    Pathosystems Resource Integration Center (PATRIC)

    More...    PATRICi    		fig|1411691.4.peg.1764

    <p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

    <p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    		U89279 Genomic DNA Translation: AAB93855.1
    U82664 Genomic DNA Translation: AAB40266.1
    U00096 Genomic DNA Translation: AAC73616.1
    AP009048 Genomic DNA Translation: BAE76292.1
    PIRiA64783
    RefSeqiNP_415047.1, NC_000913.3
    WP_001333621.1, NZ_SSZK01000024.1

    3D structure databases

    SMRiP77364
    ModBaseiSearch...

    Protein-protein interaction databases

    BioGRIDi4262012, 12 interactors
    IntActiP77364, 3 interactors
    STRINGi511145.b0514

    Chemistry databases

    ChEMBLiCHEMBL3309014

    Proteomic databases

    jPOSTiP77364
    PaxDbiP77364
    PRIDEiP77364

    Genome annotation databases

    EnsemblBacteriaiAAC73616; AAC73616; b0514
    BAE76292; BAE76292; BAE76292
    GeneIDi945129
    KEGGiecj:JW0502
    eco:b0514
    PATRICifig|1411691.4.peg.1764

    Organism-specific databases

    EchoBASE - an integrated post-genomic database for E. coli

    More...    EchoBASEi    		EB3386

    Phylogenomic databases

    eggNOGiCOG1929, Bacteria
    HOGENOMiCLU_028255_0_1_6
    InParanoidiP77364
    KOiK00865
    PhylomeDBiP77364

    Enzyme and pathway databases

    UniPathwayiUPA00864;UER00833
    BioCyciEcoCyc:GLY3KIN-MONOMER
    ECOL316407:JW0502-MONOMER
    MetaCyc:GLY3KIN-MONOMER
    BRENDAi2.7.1.165, 2165

    Miscellaneous databases

    Protein Ontology

    More...    PROi    		PR:P77364

    Family and domain databases

    Gene3Di3.40.50.10350, 1 hit
    3.90.1510.10, 1 hit
    InterProiView protein in InterPro
    IPR018193, Glyc_kinase_flavodox-like_fold
    IPR004381, Glycerate_kinase
    IPR018197, Glycerate_kinase_RE-like
    IPR036129, Glycerate_kinase_sf
    PANTHERiPTHR21599, PTHR21599, 1 hit
    PfamiView protein in Pfam
    PF02595, Gly_kinase, 1 hit
    PIRSFiPIRSF006078, GlxK, 1 hit
    SUPFAMiSSF110738, SSF110738, 1 hit
    TIGRFAMsiTIGR00045, TIGR00045, 1 hit

    ProtoNet; Automatic hierarchical classification of proteins

    More...    ProtoNeti    		Search...

    MobiDB: a database of protein disorder and mobility annotations

    More...    MobiDBi    		Search...

    <p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

    <p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiGLXK2_ECOLI
    <p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P77364
    Secondary accession number(s): Q2MBR4
    <p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: February 1, 1997
    Last modified: August 12, 2020
    This is version 143 of the entry and version 1 of the sequence. See complete history.
    <p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    <p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

    Keywords - Technical termi

    Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Uncharacterized protein families (UPF)
      List of uncharacterized protein family (UPF) entries
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