UniProtKB - P77334 (PDER_ECOLI)
Cyclic di-GMP phosphodiesterase PdeR
pdeR
Functioni
Part of a signaling cascade that regulates curli biosynthesis. The cascade is composed of two cyclic-di-GMP (c-di-GMP) control modules, in which c-di-GMP controlled by the DgcE/PdeH pair (module I) regulates the activity of the DgcM/PdeR pair (module II), which in turn regulates activity of the transcription factor MlrA and expression of the master biofilm regulator csgD (PubMed:23708798).
PdeR acts as a trigger enzyme that connects modules I and II. It inhibits DgcM and MlrA by direct interaction. Inhibition is relieved when PdeR binds and degrades c-di-GMP generated by module I (PubMed:17010156, PubMed:23708798).
2 PublicationsCatalytic activityi
- EC:3.1.4.522 Publications
GO - Molecular functioni
- cyclic-guanylate-specific phosphodiesterase activity Source: EcoCyc
- GTP binding Source: EcoCyc
GO - Biological processi
- negative regulation of cyclase activity Source: EcoCyc
- posttranscriptional regulation of gene expression Source: EcoCyc
Keywordsi
Molecular function | Hydrolase |
Ligand | c-di-GMP |
Enzyme and pathway databases
BioCyci | EcoCyc:G6639-MONOMER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:pdeR1 Publication Synonyms:gmr, yciR Ordered Locus Names:b1285, JW1278 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000168885 | 1 – 661 | Cyclic di-GMP phosphodiesterase PdeRAdd BLAST | 661 |
Proteomic databases
jPOSTi | P77334 |
PaxDbi | P77334 |
PRIDEi | P77334 |
Expressioni
Inductioni
Interactioni
Subunit structurei
Interacts with DgcM and MlrA.
1 PublicationBinary interactionsi
Protein-protein interaction databases
BioGRIDi | 4261961, 47 interactors |
DIPi | DIP-11590N |
IntActi | P77334, 6 interactors |
MINTi | P77334 |
STRINGi | 511145.b1285 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 109 – 179 | PASPROSITE-ProRule annotationAdd BLAST | 71 | |
Domaini | 265 – 397 | GGDEFPROSITE-ProRule annotationAdd BLAST | 133 | |
Domaini | 406 – 658 | EALPROSITE-ProRule annotationAdd BLAST | 253 |
Phylogenomic databases
eggNOGi | COG5001, Bacteria |
HOGENOMi | CLU_000445_70_50_6 |
InParanoidi | P77334 |
OMAi | RFCCAFQ |
PhylomeDBi | P77334 |
Family and domain databases
CDDi | cd01948, EAL, 1 hit cd01949, GGDEF, 1 hit cd00130, PAS, 1 hit |
Gene3Di | 3.20.20.450, 1 hit 3.30.70.270, 1 hit |
InterProi | View protein in InterPro IPR001633, EAL_dom IPR035919, EAL_sf IPR000160, GGDEF_dom IPR029787, Nucleotide_cyclase IPR000014, PAS IPR035965, PAS-like_dom_sf IPR043128, Rev_trsase/Diguanyl_cyclase |
Pfami | View protein in Pfam PF00563, EAL, 1 hit PF00990, GGDEF, 1 hit PF13426, PAS_9, 1 hit |
SMARTi | View protein in SMART SM00052, EAL, 1 hit SM00267, GGDEF, 1 hit SM00091, PAS, 1 hit |
SUPFAMi | SSF141868, SSF141868, 1 hit SSF55073, SSF55073, 1 hit SSF55785, SSF55785, 1 hit |
TIGRFAMsi | TIGR00254, GGDEF, 1 hit TIGR00229, sensory_box, 1 hit |
PROSITEi | View protein in PROSITE PS50883, EAL, 1 hit PS50887, GGDEF, 1 hit PS50112, PAS, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MKTVRESTTL YNFLGSHNPY WRLTESSDVL RFSTTETTEP DRTLQLSAEQ
60 70 80 90 100
AARIREMTVI TSSLMMSLTV DESDLSVHLV GRKINKREWA GNASAWHDTP
110 120 130 140 150
AVARDLSHGL SFAEQVVSEA HSAIVILDSR GNIQRFNRLC EDYTGLKEHD
160 170 180 190 200
VIGQSVFKLF MSRREAAASR RNNRVFFRSG NAYEVELWIP TCKGQRLFLF
210 220 230 240 250
RNKFVHSGSG KNEIFLICSG TDITEERRAQ ERLRILANTD SITGLPNRNA
260 270 280 290 300
MQDLIDHAIN HADNNKVGVV YLDLDNFKKV NDAYGHLFGD QLLRDVSLAI
310 320 330 340 350
LSCLEHDQVL ARPGGDEFLV LASNTSQSAL EAMASRILTR LRLPFRIGLI
360 370 380 390 400
EVYTSCSVGI ALSPEHGSDS TAIIRHADTA MYTAKEGGRG QFCVFTPEMN
410 420 430 440 450
QRVFEYLWLD TNLRKALEND QLVIHYQPKI TWRGEVRSLE ALVRWQSPER
460 470 480 490 500
GLIPPLDFIS YAEESGLIVP LGRWVILDVV RQVAKWRDKG INLRVAVNIS
510 520 530 540 550
ARQLADQTIF TALKQVLQEL NFEYCPIDVE LTESCLIEND ELALSVIQQF
560 570 580 590 600
SQLGAQVHLD DFGTGYSSLS QLARFPIDAI KLDQVFVRDI HKQPVSQSLV
610 620 630 640 650
RAIVAVAQAL NLQVIAEGVE SAKEDAFLTK NGINERQGFL FAKPMPAVAF
660
ERWYKRYLKR A
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 4 | V → L in L40788 (Ref. 4) Curated | 1 | |
Sequence conflicti | 113 | A → P in L40788 (Ref. 4) Curated | 1 | |
Sequence conflicti | 175 | V → L in L40788 (Ref. 4) Curated | 1 | |
Sequence conflicti | 256 | D → H in L40788 (Ref. 4) Curated | 1 | |
Sequence conflicti | 344 | P → G in L40788 (Ref. 4) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74367.1 AP009048 Genomic DNA Translation: BAA14839.1 L40788 Genomic DNA No translation available. |
PIRi | H64876 |
RefSeqi | NP_415801.1, NC_000913.3 WP_000859945.1, NZ_SSZK01000012.1 |
Genome annotation databases
EnsemblBacteriai | AAC74367; AAC74367; b1285 BAA14839; BAA14839; BAA14839 |
GeneIDi | 66674889 945868 |
KEGGi | ecj:JW1278 eco:b1285 |
PATRICi | fig|1411691.4.peg.994 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74367.1 AP009048 Genomic DNA Translation: BAA14839.1 L40788 Genomic DNA No translation available. |
PIRi | H64876 |
RefSeqi | NP_415801.1, NC_000913.3 WP_000859945.1, NZ_SSZK01000012.1 |
3D structure databases
AlphaFoldDBi | P77334 |
SMRi | P77334 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4261961, 47 interactors |
DIPi | DIP-11590N |
IntActi | P77334, 6 interactors |
MINTi | P77334 |
STRINGi | 511145.b1285 |
Proteomic databases
jPOSTi | P77334 |
PaxDbi | P77334 |
PRIDEi | P77334 |
Protocols and materials databases
DNASUi | 945868 |
Genome annotation databases
EnsemblBacteriai | AAC74367; AAC74367; b1285 BAA14839; BAA14839; BAA14839 |
GeneIDi | 66674889 945868 |
KEGGi | ecj:JW1278 eco:b1285 |
PATRICi | fig|1411691.4.peg.994 |
Organism-specific databases
EchoBASEi | EB3191 |
Phylogenomic databases
eggNOGi | COG5001, Bacteria |
HOGENOMi | CLU_000445_70_50_6 |
InParanoidi | P77334 |
OMAi | RFCCAFQ |
PhylomeDBi | P77334 |
Enzyme and pathway databases
BioCyci | EcoCyc:G6639-MONOMER |
Miscellaneous databases
PROi | PR:P77334 |
Family and domain databases
CDDi | cd01948, EAL, 1 hit cd01949, GGDEF, 1 hit cd00130, PAS, 1 hit |
Gene3Di | 3.20.20.450, 1 hit 3.30.70.270, 1 hit |
InterProi | View protein in InterPro IPR001633, EAL_dom IPR035919, EAL_sf IPR000160, GGDEF_dom IPR029787, Nucleotide_cyclase IPR000014, PAS IPR035965, PAS-like_dom_sf IPR043128, Rev_trsase/Diguanyl_cyclase |
Pfami | View protein in Pfam PF00563, EAL, 1 hit PF00990, GGDEF, 1 hit PF13426, PAS_9, 1 hit |
SMARTi | View protein in SMART SM00052, EAL, 1 hit SM00267, GGDEF, 1 hit SM00091, PAS, 1 hit |
SUPFAMi | SSF141868, SSF141868, 1 hit SSF55073, SSF55073, 1 hit SSF55785, SSF55785, 1 hit |
TIGRFAMsi | TIGR00254, GGDEF, 1 hit TIGR00229, sensory_box, 1 hit |
PROSITEi | View protein in PROSITE PS50883, EAL, 1 hit PS50887, GGDEF, 1 hit PS50112, PAS, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PDER_ECOLI | |
Accessioni | P77334Primary (citable) accession number: P77334 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | February 1, 1997 | |
Last modified: | May 25, 2022 | |
This is version 162 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |