UniProtKB - P77302 (DGCM_ECOLI)
Diguanylate cyclase DgcM
dgcM
Functioni
Part of a signaling cascade that regulates curli biosynthesis. The cascade is composed of two cyclic-di-GMP (c-di-GMP) control modules, in which c-di-GMP controlled by the DgcE/PdeH pair (module I) regulates the activity of the DgcM/PdeR pair (module II), which in turn regulates activity of the transcription factor MlrA and expression of the master biofilm regulator csgD (PubMed:23708798).
DgcM stimulates activity of MlrA by direct interaction, leading to the transcription of csgD. It also catalyzes the synthesis of c-di-GMP via the condensation of 2 GTP molecules, which contributes to the c-di-GMP pool generated by module I in a positive feedback loop. Production of c-di-GMP contributes to but is not essential for MlrA activation (PubMed:17010156, PubMed:23708798).
2 PublicationsCatalytic activityi
- EC:2.7.7.652 Publications
Cofactori
Activity regulationi
: 3',5'-cyclic di-GMP biosynthesis Pathwayi
This protein is involved in the pathway 3',5'-cyclic di-GMP biosynthesis, which is part of Purine metabolism.CuratedView all proteins of this organism that are known to be involved in the pathway 3',5'-cyclic di-GMP biosynthesis and in Purine metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 291 | MagnesiumBy similarity | 1 | |
Sitei | 296 | Transition state stabilizerSequence analysis | 1 | |
Binding sitei | 299 | SubstrateBy similarity | 1 | |
Binding sitei | 304 | Substrate; via carbonyl oxygenBy similarity | 1 | |
Binding sitei | 308 | SubstrateBy similarity | 1 | |
Active sitei | 334 | Proton acceptorSequence analysis | 1 | |
Metal bindingi | 334 | MagnesiumBy similarity | 1 |
GO - Molecular functioni
- diguanylate cyclase activity Source: EcoCyc
- GTP binding Source: UniProtKB-KW
- identical protein binding Source: EcoCyc
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- cell adhesion involved in single-species biofilm formation Source: GO_Central
- negative regulation of bacterial-type flagellum-dependent cell motility Source: GO_Central
- positive regulation of DNA-binding transcription factor activity Source: EcoCyc
- positive regulation of single-species biofilm formation on inanimate substrate Source: EcoCyc
Keywordsi
Molecular function | Transferase |
Ligand | GTP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:G6673-MONOMER |
UniPathwayi | UPA00599 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:dgcM1 Publication Synonyms:ydaM Ordered Locus Names:b1341, JW5206 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 334 – 335 | EE → AA: No longer complements a dgcM disruption, loss of csgB induction. 1 Publication | 2 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000201313 | 1 – 410 | Diguanylate cyclase DgcMAdd BLAST | 410 |
Proteomic databases
jPOSTi | P77302 |
PaxDbi | P77302 |
PRIDEi | P77302 |
Expressioni
Inductioni
Interactioni
Subunit structurei
Binary interactionsi
P77302
With | #Exp. | IntAct |
---|---|---|
itself | 3 | EBI-544662,EBI-544662 |
mlrA [P33358] | 2 | EBI-544662,EBI-1127668 |
pdeR [P77334] | 5 | EBI-544662,EBI-548149 |
GO - Molecular functioni
- identical protein binding Source: EcoCyc
Protein-protein interaction databases
BioGRIDi | 4259650, 19 interactors |
DIPi | DIP-28053N |
IntActi | P77302, 7 interactors |
MINTi | P77302 |
STRINGi | 511145.b1341 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 3 – 70 | PAS 1Sequence analysisAdd BLAST | 68 | |
Domaini | 129 – 198 | PAS 2Sequence analysisAdd BLAST | 70 | |
Domaini | 199 – 251 | PACPROSITE-ProRule annotationAdd BLAST | 53 | |
Domaini | 283 – 410 | GGDEFPROSITE-ProRule annotationAdd BLAST | 128 |
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | COG3706, Bacteria |
HOGENOMi | CLU_000445_11_4_6 |
InParanoidi | P77302 |
OMAi | HTWEINT |
PhylomeDBi | P77302 |
Family and domain databases
CDDi | cd01949, GGDEF, 1 hit cd00130, PAS, 1 hit |
Gene3Di | 3.30.70.270, 1 hit |
InterProi | View protein in InterPro IPR000160, GGDEF_dom IPR029787, Nucleotide_cyclase IPR000014, PAS IPR000700, PAS-assoc_C IPR035965, PAS-like_dom_sf IPR013656, PAS_4 IPR043128, Rev_trsase/Diguanyl_cyclase |
Pfami | View protein in Pfam PF00990, GGDEF, 1 hit PF08448, PAS_4, 1 hit |
SMARTi | View protein in SMART SM00267, GGDEF, 1 hit SM00091, PAS, 2 hits |
SUPFAMi | SSF55073, SSF55073, 1 hit SSF55785, SSF55785, 1 hit |
TIGRFAMsi | TIGR00254, GGDEF, 1 hit TIGR00229, sensory_box, 1 hit |
PROSITEi | View protein in PROSITE PS50887, GGDEF, 1 hit PS50113, PAC, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MITHNFNTLD LLTSPVWIVS PFEEQLIYAN SAAKLLMQDL TFSQLRTGPY
60 70 80 90 100
SVSSQKELPK YLSDLQNQHD IIEILTVQRK EEETALSCRL VLRKLTETEP
110 120 130 140 150
VIIFEGIEAP ATLGLKASRS ANYQRKKQGF YARFFLTNSA PMLLIDPSRD
160 170 180 190 200
GQIVDANLAA LNFYGYNHET MCQKHTWEIN MLGRRVMPIM HEISHLPGGH
210 220 230 240 250
KPLNFVHKLA DGSTRHVQTY AGPIEIYGDK LMLCIVHDIT EQKRLEEQLE
260 270 280 290 300
HAAHHDAMTG LLNRRQFYHI TEPGQMQHLA IAQDYSLLLI DTDRFKHIND
310 320 330 340 350
LYGHSKGDEV LCALARTLES CARKGDLVFR WGGEEFVLLL PRTPLDTALS
360 370 380 390 400
LAETIRVSVA KVSISGLPRF TVSIGVAHHE GNESIDELFK RVDDALYRAK
410
NDGRNRVLAA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74423.2 AP009048 Genomic DNA Translation: BAA14945.2 |
PIRi | H64883 |
RefSeqi | NP_415857.2, NC_000913.3 WP_000628058.1, NZ_SSZK01000012.1 |
Genome annotation databases
EnsemblBacteriai | AAC74423; AAC74423; b1341 BAA14945; BAA14945; BAA14945 |
GeneIDi | 945909 |
KEGGi | ecj:JW5206 eco:b1341 |
PATRICi | fig|1411691.4.peg.936 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74423.2 AP009048 Genomic DNA Translation: BAA14945.2 |
PIRi | H64883 |
RefSeqi | NP_415857.2, NC_000913.3 WP_000628058.1, NZ_SSZK01000012.1 |
3D structure databases
AlphaFoldDBi | P77302 |
SMRi | P77302 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4259650, 19 interactors |
DIPi | DIP-28053N |
IntActi | P77302, 7 interactors |
MINTi | P77302 |
STRINGi | 511145.b1341 |
Proteomic databases
jPOSTi | P77302 |
PaxDbi | P77302 |
PRIDEi | P77302 |
Genome annotation databases
EnsemblBacteriai | AAC74423; AAC74423; b1341 BAA14945; BAA14945; BAA14945 |
GeneIDi | 945909 |
KEGGi | ecj:JW5206 eco:b1341 |
PATRICi | fig|1411691.4.peg.936 |
Organism-specific databases
EchoBASEi | EB3138 |
Phylogenomic databases
eggNOGi | COG3706, Bacteria |
HOGENOMi | CLU_000445_11_4_6 |
InParanoidi | P77302 |
OMAi | HTWEINT |
PhylomeDBi | P77302 |
Enzyme and pathway databases
UniPathwayi | UPA00599 |
BioCyci | EcoCyc:G6673-MONOMER |
Miscellaneous databases
PROi | PR:P77302 |
Family and domain databases
CDDi | cd01949, GGDEF, 1 hit cd00130, PAS, 1 hit |
Gene3Di | 3.30.70.270, 1 hit |
InterProi | View protein in InterPro IPR000160, GGDEF_dom IPR029787, Nucleotide_cyclase IPR000014, PAS IPR000700, PAS-assoc_C IPR035965, PAS-like_dom_sf IPR013656, PAS_4 IPR043128, Rev_trsase/Diguanyl_cyclase |
Pfami | View protein in Pfam PF00990, GGDEF, 1 hit PF08448, PAS_4, 1 hit |
SMARTi | View protein in SMART SM00267, GGDEF, 1 hit SM00091, PAS, 2 hits |
SUPFAMi | SSF55073, SSF55073, 1 hit SSF55785, SSF55785, 1 hit |
TIGRFAMsi | TIGR00254, GGDEF, 1 hit TIGR00229, sensory_box, 1 hit |
PROSITEi | View protein in PROSITE PS50887, GGDEF, 1 hit PS50113, PAC, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DGCM_ECOLI | |
Accessioni | P77302Primary (citable) accession number: P77302 Secondary accession number(s): P76846 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 27, 2002 |
Last sequence update: | December 4, 2007 | |
Last modified: | May 25, 2022 | |
This is version 174 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways