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UniProtKB - P77265 (MDLA_ECOLI)
Protein
Multidrug resistance-like ATP-binding protein MdlA
Gene
mdlA
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalytic activityi
- ATP + H(2)O + xenobiotic(Side 1) = ADP + phosphate + xenobiotic(Side 2). EC:7.6.2.2
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 369 – 376 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ABC-type xenobiotic transporter activity Source: UniProtKB-EC
- ATPase-coupled transmembrane transporter activity Source: GO_Central
- ATP binding Source: EcoCyc
GO - Biological processi
- transmembrane transport Source: GO_Central
Keywordsi
Molecular function | Translocase |
Biological process | Transport |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:MDLA-MONOMER |
Protein family/group databases
TCDBi | 3.A.1.106.13, the atp-binding cassette (abc) superfamily |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:mdlA Synonyms:mdl Ordered Locus Names:b0448, JW0438 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane 1 Publication; Multi-pass membrane protein PROSITE-ProRule annotation1 Publication
Plasma Membrane
- plasma membrane Source: EcoCyc
Other locations
- integral component of membrane Source: EcoCyc
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 23 – 43 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 53 – 73 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 134 – 154 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 155 – 175 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 248 – 268 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 280 – 300 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000092492 | 1 – 590 | Multidrug resistance-like ATP-binding protein MdlAAdd BLAST | 590 |
Proteomic databases
PaxDbi | P77265 |
PRIDEi | P77265 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4261677, 157 interactors |
DIPi | DIP-10173N |
IntActi | P77265, 1 interactor |
STRINGi | 511145.b0448 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 18 – 303 | ABC transmembrane type-1PROSITE-ProRule annotationAdd BLAST | 286 | |
Domaini | 337 – 570 | ABC transporterPROSITE-ProRule annotationAdd BLAST | 234 |
Sequence similaritiesi
Belongs to the ABC transporter superfamily. Drug exporter-2 (TC 3.A.1.117) family. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG1132, Bacteria |
HOGENOMi | CLU_000604_84_6_6 |
InParanoidi | P77265 |
OMAi | RVMHDLR |
PhylomeDBi | P77265 |
Family and domain databases
Gene3Di | 1.20.1560.10, 1 hit 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR011527, ABC1_TM_dom IPR036640, ABC1_TM_sf IPR003439, ABC_transporter-like_ATP-bd IPR017871, ABC_transporter-like_CS IPR027417, P-loop_NTPase IPR039421, Type_1_exporter |
PANTHERi | PTHR24221, PTHR24221, 1 hit |
Pfami | View protein in Pfam PF00664, ABC_membrane, 1 hit PF00005, ABC_tran, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF90123, SSF90123, 1 hit |
PROSITEi | View protein in PROSITE PS50929, ABC_TM1F, 1 hit PS00211, ABC_TRANSPORTER_1, 1 hit PS50893, ABC_TRANSPORTER_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P77265-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRLFAQLSWY FRREWRRYLG AVALLVIIAM LQLVPPKVVG IVVDGVTEQH
60 70 80 90 100
FTTGQILMWI ATMVLIAVVV YLLRYVWRVL LFGASYQLAV ELREDYYRQL
110 120 130 140 150
SRQHPEFYLR HRTGDLMARA TNDVDRVVFA AGEGVLTLVD SLVMGCAVLI
160 170 180 190 200
MMSTQISWQL TLFSLLPMPV MAIMIKRNGD ALHERFKLAQ AAFSSLNDRT
210 220 230 240 250
QESLTSIRMI KAFGLEDRQS ALFAADAEDT GKKNMRVARI DARFDPTIYI
260 270 280 290 300
AIGMANLLAI GGGSWMVVQG SLTLGQLTSF MMYLGLMIWP MLALAWMFNI
310 320 330 340 350
VERGSAAYSR IRAMLAEAPV VNDGSEPVPE GRGELDVNIH QFTYPQTDHP
360 370 380 390 400
ALENVNFALK PGQMLGICGP TGSGKSTLLS LIQRHFDVSE GDIRFHDIPL
410 420 430 440 450
TKLQLDSWRS RLAVVSQTPF LFSDTVANNI ALGCPNATQQ EIEHVARLAS
460 470 480 490 500
VHDDILRLPQ GYDTEVGERG VMLSGGQKQR ISIARALLVN AEILILDDAL
510 520 530 540 550
SAVDGRTEHQ ILHNLRQWGQ GRTVIISAHR LSALTEASEI IVMQHGHIAQ
560 570 580 590
RGNHDVLAQQ SGWYRDMYRY QQLEAALDDA PENREEAVDA
Sequence cautioni
The sequence AAC36870 differs from that shown. Reason: Frameshift. Fuses together mdlA and mdlB.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 145 | G → A in AAC36870 (PubMed:7904973).Curated | 1 | |
Sequence conflicti | 234 – 235 | NM → KL in AAC36870 (PubMed:7904973).Curated | 2 | |
Sequence conflicti | 318 | A → R in AAC36870 (PubMed:7904973).Curated | 1 | |
Sequence conflicti | 371 | T → N in AAC36870 (PubMed:7904973).Curated | 1 | |
Sequence conflicti | 390 | E → P in AAC36870 (PubMed:7904973).Curated | 1 | |
Sequence conflicti | 410 – 411 | SR → TG in AAC36870 (PubMed:7904973).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L08627 Unassigned DNA Translation: AAC36870.1 Frameshift. U82664 Genomic DNA Translation: AAB40204.1 U00096 Genomic DNA Translation: AAC73551.1 AP009048 Genomic DNA Translation: BAE76228.1 |
PIRi | H64774 |
RefSeqi | NP_414982.1, NC_000913.3 WP_001235649.1, NZ_SSZK01000009.1 |
Genome annotation databases
EnsemblBacteriai | AAC73551; AAC73551; b0448 BAE76228; BAE76228; BAE76228 |
GeneIDi | 945092 |
KEGGi | ecj:JW0438 eco:b0448 |
PATRICi | fig|1411691.4.peg.1828 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L08627 Unassigned DNA Translation: AAC36870.1 Frameshift. U82664 Genomic DNA Translation: AAB40204.1 U00096 Genomic DNA Translation: AAC73551.1 AP009048 Genomic DNA Translation: BAE76228.1 |
PIRi | H64774 |
RefSeqi | NP_414982.1, NC_000913.3 WP_001235649.1, NZ_SSZK01000009.1 |
3D structure databases
AlphaFoldDBi | P77265 |
SMRi | P77265 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4261677, 157 interactors |
DIPi | DIP-10173N |
IntActi | P77265, 1 interactor |
STRINGi | 511145.b0448 |
Protein family/group databases
TCDBi | 3.A.1.106.13, the atp-binding cassette (abc) superfamily |
Proteomic databases
PaxDbi | P77265 |
PRIDEi | P77265 |
Genome annotation databases
EnsemblBacteriai | AAC73551; AAC73551; b0448 BAE76228; BAE76228; BAE76228 |
GeneIDi | 945092 |
KEGGi | ecj:JW0438 eco:b0448 |
PATRICi | fig|1411691.4.peg.1828 |
Organism-specific databases
EchoBASEi | EB1579 |
Phylogenomic databases
eggNOGi | COG1132, Bacteria |
HOGENOMi | CLU_000604_84_6_6 |
InParanoidi | P77265 |
OMAi | RVMHDLR |
PhylomeDBi | P77265 |
Enzyme and pathway databases
BioCyci | EcoCyc:MDLA-MONOMER |
Miscellaneous databases
PROi | PR:P77265 |
Family and domain databases
Gene3Di | 1.20.1560.10, 1 hit 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR011527, ABC1_TM_dom IPR036640, ABC1_TM_sf IPR003439, ABC_transporter-like_ATP-bd IPR017871, ABC_transporter-like_CS IPR027417, P-loop_NTPase IPR039421, Type_1_exporter |
PANTHERi | PTHR24221, PTHR24221, 1 hit |
Pfami | View protein in Pfam PF00664, ABC_membrane, 1 hit PF00005, ABC_tran, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF90123, SSF90123, 1 hit |
PROSITEi | View protein in PROSITE PS50929, ABC_TM1F, 1 hit PS00211, ABC_TRANSPORTER_1, 1 hit PS50893, ABC_TRANSPORTER_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MDLA_ECOLI | |
Accessioni | P77265Primary (citable) accession number: P77265 Secondary accession number(s): P30751, Q2MBX8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1999 |
Last sequence update: | February 1, 1997 | |
Last modified: | May 25, 2022 | |
This is version 173 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families