UniProtKB - P77258 (NEMA_ECOLI)
Protein
N-ethylmaleimide reductase
Gene
nemA
Organism
Escherichia coli (strain K12)
Status
Functioni
Involved in the degradation of toxic compounds (PubMed:9013822, PubMed:15184158, PubMed:17504490, PubMed:23527133, PubMed:23506073, PubMed:23536188). Can use a variety of substrates, including the nitrate ester explosives glycerol trinitrate (GTN) and pentaerythritol tetranitrate (PETN), chromate and various electrophiles such as quinones (PubMed:15184158, PubMed:23527133, PubMed:23506073). Involved in resistance to hypochlorous acid (HOCl), which is the active component of household bleach and a powerful antimicrobial during the innate immune response (PubMed:23536188). Catalyzes the reduction of N-ethylmaleimide (NEM) to N-ethylsuccinimide (PubMed:9013822, PubMed:23506073). Together with NfsA and NfsB, can use the nitroaromatic explosive 2,4,6-trinitrotoluene (TNT) (PubMed:17504490).6 Publications
Catalytic activityi
Cofactori
FMNBy similarity
Kineticsi
kcat is 354000 sec(-1) with NEM as substrate. kcat is 123000 sec(-1) with ubiquinone 0 as substrate. kcat is 14200 sec(-1) with menadione as substrate (PubMed:23506073). kcat is 2.1 sec(-1) with chromate as substrate in the presence of NADPH. kcat is 1 sec(-1) with chromate as substrate in the presence of NADH (PubMed:23527133).2 Publications
- KM=27.13 µM for NEM1 Publication
- KM=23 µM for chromate (in the presence of NADPH)1 Publication
- KM=16 µM for chromate (in the presence of NADH)1 Publication
- KM=0.29 mM for menadione1 Publication
- KM=0.31 mM for ubiquinone 01 Publication
- KM=0.78 mM for ubiquinone 101 Publication
- KM=6.43 mM for acrolein1 Publication
- KM=11.52 mM for menaquinone1 Publication
- KM=25.63 mM for methylglyoxal1 Publication
- KM=318 mM for glyoxal1 Publication
- KM=480 mM for glycolaldehyde1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 101 | FMNBy similarity | 1 | |
Active sitei | 187 | Proton donorBy similarity | 1 | |
Binding sitei | 234 | FMNBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 324 – 325 | FMNBy similarity | 2 |
GO - Molecular functioni
- chromate reductase activity Source: EcoCyc
- FMN binding Source: EcoCyc
- N-ethylmaleimide reductase activity Source: EcoCyc
- oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Source: EcoCyc
GO - Biological processi
- 2,4,6-trinitrotoluene catabolic process Source: EcoCyc
- nitroglycerin metabolic process Source: EcoCyc
- xenobiotic metabolic process Source: EcoCyc
Keywordsi
Molecular function | Oxidoreductase |
Ligand | Flavoprotein, FMN, NADP |
Enzyme and pathway databases
BioCyci | EcoCyc:G6890-MONOMER MetaCyc:G6890-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: N-ethylmaleimide reductase1 Publication (EC:1.3.1.-2 Publications)Short name: NEM reductase1 Publication Alternative name(s): N-ethylmaleimide reducing enzyme |
Gene namesi | Name:nemA1 Publication Synonyms:ydhN Ordered Locus Names:b1650, JW1642 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000194481 | 1 – 365 | N-ethylmaleimide reductaseAdd BLAST | 365 |
Proteomic databases
jPOSTi | P77258 |
PaxDbi | P77258 |
PRIDEi | P77258 |
2D gel databases
SWISS-2DPAGEi | P77258 |
Expressioni
Inductioni
Interactioni
Protein-protein interaction databases
BioGRIDi | 4259388, 46 interactors |
IntActi | P77258, 3 interactors |
STRINGi | 511145.b1650 |
Family & Domainsi
Sequence similaritiesi
Belongs to the NADH:flavin oxidoreductase/NADH oxidase family.Curated
Phylogenomic databases
eggNOGi | COG1902, Bacteria |
HOGENOMi | CLU_012153_0_2_6 |
InParanoidi | P77258 |
PhylomeDBi | P77258 |
Family and domain databases
Gene3Di | 3.20.20.70, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR001155, OxRdtase_FMN_N |
Pfami | View protein in Pfam PF00724, Oxidored_FMN, 1 hit |
i Sequence
Sequence statusi: Complete.
P77258-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSSEKLYSPL KVGAITAANR IFMAPLTRLR SIEPGDIPTP LMAEYYRQRA
60 70 80 90 100
SAGLIISEAT QISAQAKGYA GAPGIHSPEQ IAAWKKITAG VHAENGHMAV
110 120 130 140 150
QLWHTGRISH ASLQPGGQAP VAPSALSAGT RTSLRDENGQ AIRVETSMPR
160 170 180 190 200
ALELEEIPGI VNDFRQAIAN AREAGFDLVE LHSAHGYLLH QFLSPSSNHR
210 220 230 240 250
TDQYGGSVEN RARLVLEVVD AGIEEWGADR IGIRVSPIGT FQNTDNGPNE
260 270 280 290 300
EADALYLIEQ LGKRGIAYLH MSEPDWAGGE PYTDAFREKV RARFHGPIIG
310 320 330 340 350
AGAYTVEKAE TLIGKGLIDA VAFGRDWIAN PDLVARLQRK AELNPQRAES
360
FYGGGAEGYT DYPTL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D86931 Genomic DNA Translation: BAA13186.1 U00096 Genomic DNA Translation: AAC74722.1 AP009048 Genomic DNA Translation: BAE76493.1 |
PIRi | JC5605 |
RefSeqi | NP_416167.1, NC_000913.3 WP_000093589.1, NZ_SSZK01000001.1 |
Genome annotation databases
EnsemblBacteriai | AAC74722; AAC74722; b1650 BAE76493; BAE76493; BAE76493 |
GeneIDi | 57731045 946164 |
KEGGi | ecj:JW1642 eco:b1650 |
PATRICi | fig|1411691.4.peg.609 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D86931 Genomic DNA Translation: BAA13186.1 U00096 Genomic DNA Translation: AAC74722.1 AP009048 Genomic DNA Translation: BAE76493.1 |
PIRi | JC5605 |
RefSeqi | NP_416167.1, NC_000913.3 WP_000093589.1, NZ_SSZK01000001.1 |
3D structure databases
SMRi | P77258 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4259388, 46 interactors |
IntActi | P77258, 3 interactors |
STRINGi | 511145.b1650 |
2D gel databases
SWISS-2DPAGEi | P77258 |
Proteomic databases
jPOSTi | P77258 |
PaxDbi | P77258 |
PRIDEi | P77258 |
Genome annotation databases
EnsemblBacteriai | AAC74722; AAC74722; b1650 BAE76493; BAE76493; BAE76493 |
GeneIDi | 57731045 946164 |
KEGGi | ecj:JW1642 eco:b1650 |
PATRICi | fig|1411691.4.peg.609 |
Organism-specific databases
EchoBASEi | EB3316 |
Phylogenomic databases
eggNOGi | COG1902, Bacteria |
HOGENOMi | CLU_012153_0_2_6 |
InParanoidi | P77258 |
PhylomeDBi | P77258 |
Enzyme and pathway databases
BioCyci | EcoCyc:G6890-MONOMER MetaCyc:G6890-MONOMER |
Miscellaneous databases
PROi | PR:P77258 |
Family and domain databases
Gene3Di | 3.20.20.70, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR001155, OxRdtase_FMN_N |
Pfami | View protein in Pfam PF00724, Oxidored_FMN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NEMA_ECOLI | |
Accessioni | P77258Primary (citable) accession number: P77258 Secondary accession number(s): Q2MB63 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | February 1, 1997 | |
Last modified: | February 10, 2021 | |
This is version 140 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families