UniProtKB - P77247 (HXPB_ECOLI)
Hexitol phosphatase B
hxpB
Functioni
Sugar-phosphate phosphohydrolase that catalyzes the dephosphorylation of D-mannitol 1-phosphate and D-sorbitol 6-phosphate (PubMed:27941785).
Also catalyzes the dephosphorylation of 2-deoxyglucose 6-phosphate (2dGlu6P); this is a biologically important activity in vivo since it contributes to the elimination of this toxic compound and plays an important role in the resistance of E.coli to 2-deoxyglucose (PubMed:16990279).
To a lesser extent, is also able to dephosphorylate mannose 6-phosphate (Man6P), erythrose-4-phosphate, 2-deoxyribose-5-phosphate (2dRib5P), ribose-5-phosphate (Rib5P) and glucose-6-phosphate (Glu6P) in vitro (PubMed:16990279).
3 PublicationsCatalytic activityi
- Sugar phosphate + H(2)O = sugar + phosphate.1 Publication EC:3.1.3.23
Cofactori
Kineticsi
- KM=0.61 mM for 2-deoxyglucose-6-P (with manganese ions as cofactor and at pH 9)1 Publication
- KM=2.5 mM for 2-deoxyribose-5-P (with zinc ions as cofactor and at pH 9)1 Publication
- KM=2.6 mM for ribose-5-P (with zinc ions as cofactor and at pH 9)1 Publication
- KM=3.6 mM for glucose-6-P (with zinc ions as cofactor and at pH 9)1 Publication
- KM=4.7 mM for mannose-6-P (with zinc ions as cofactor and at pH 9)1 Publication
pH dependencei
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 13 | NucleophileBy similarity | 1 | |
Metal bindingi | 13 | Divalent metal cation1 Publication | 1 | |
Active sitei | 15 | Proton donorBy similarity | 1 | |
Metal bindingi | 15 | Divalent metal cation; via carbonyl oxygen1 Publication | 1 | |
Binding sitei | 148 | Substrate1 Publication | 1 | |
Metal bindingi | 173 | Divalent metal cation1 Publication | 1 |
GO - Molecular functioni
- 2-deoxyglucose-6-phosphatase activity Source: EcoliWiki
- glucose-6-phosphatase activity Source: EcoliWiki
- magnesium ion binding Source: UniProtKB
- mannitol-1-phosphatase activity Source: EcoCyc
- metal ion binding Source: EcoliWiki
- phosphatase activity Source: EcoliWiki
- sorbitol-6-phosphatase activity Source: EcoCyc
- sugar-phosphatase activity Source: EcoliWiki
GO - Biological processi
- carbohydrate metabolic process Source: UniProtKB-KW
Keywordsi
Molecular function | Hydrolase |
Biological process | Carbohydrate metabolism |
Ligand | Cobalt, Magnesium, Manganese, Metal-binding, Zinc |
Enzyme and pathway databases
BioCyci | EcoCyc:G6932-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Hexitol phosphatase B1 PublicationAlternative name(s): |
Gene namesi | Name:hxpB1 Publication Synonyms:yniCImported Ordered Locus Names:b1727, JW1716 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 13 | D → A: Loss of phosphatase activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000108061 | 1 – 222 | Hexitol phosphatase BAdd BLAST | 222 |
Proteomic databases
jPOSTi | P77247 |
PaxDbi | P77247 |
PRIDEi | P77247 |
2D gel databases
SWISS-2DPAGEi | P77247 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4263414, 9 interactors |
DIPi | DIP-12777N |
IntActi | P77247, 8 interactors |
STRINGi | 511145.b1727 |
Structurei
Secondary structure
3D structure databases
SMRi | P77247 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P77247 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 13 – 15 | Substrate binding1 Publication | 3 | |
Regioni | 115 – 116 | Substrate binding1 Publication | 2 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0637, Bacteria |
HOGENOMi | CLU_045011_13_1_6 |
InParanoidi | P77247 |
PhylomeDBi | P77247 |
Family and domain databases
Gene3Di | 1.10.150.240, 1 hit 3.40.50.1000, 1 hit |
InterProi | View protein in InterPro IPR036412, HAD-like_sf IPR006439, HAD-SF_hydro_IA IPR041492, HAD_2 IPR023214, HAD_sf IPR023198, PGP-like_dom2 |
Pfami | View protein in Pfam PF13419, HAD_2, 1 hit |
PRINTSi | PR00413, HADHALOGNASE |
SUPFAMi | SSF56784, SSF56784, 1 hit |
TIGRFAMsi | TIGR01509, HAD-SF-IA-v3, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MSTPRQILAA IFDMDGLLID SEPLWDRAEL DVMASLGVDI SRRNELPDTL
60 70 80 90 100
GLRIDMVVDL WYARQPWNGP SRQEVVERVI ARAISLVEET RPLLPGVREA
110 120 130 140 150
VALCKEQGLL VGLASASPLH MLEKVLTMFD LRDSFDALAS AEKLPYSKPH
160 170 180 190 200
PQVYLDCAAK LGVDPLTCVA LEDSVNGMIA SKAARMRSIV VPAPEAQNDP
210 220
RFVLADVKLS SLTELTAKDL LG
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74797.1 AP009048 Genomic DNA Translation: BAA15508.1 |
PIRi | G64931 |
RefSeqi | NP_416241.1, NC_000913.3 WP_000106833.1, NZ_SSZK01000001.1 |
Genome annotation databases
EnsemblBacteriai | AAC74797; AAC74797; b1727 BAA15508; BAA15508; BAA15508 |
GeneIDi | 945632 |
KEGGi | ecj:JW1716 eco:b1727 |
PATRICi | fig|1411691.4.peg.529 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74797.1 AP009048 Genomic DNA Translation: BAA15508.1 |
PIRi | G64931 |
RefSeqi | NP_416241.1, NC_000913.3 WP_000106833.1, NZ_SSZK01000001.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1TE2 | X-ray | 1.76 | A/B | 1-222 | [»] | |
SMRi | P77247 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4263414, 9 interactors |
DIPi | DIP-12777N |
IntActi | P77247, 8 interactors |
STRINGi | 511145.b1727 |
2D gel databases
SWISS-2DPAGEi | P77247 |
Proteomic databases
jPOSTi | P77247 |
PaxDbi | P77247 |
PRIDEi | P77247 |
Protocols and materials databases
DNASUi | 945632 |
Genome annotation databases
EnsemblBacteriai | AAC74797; AAC74797; b1727 BAA15508; BAA15508; BAA15508 |
GeneIDi | 945632 |
KEGGi | ecj:JW1716 eco:b1727 |
PATRICi | fig|1411691.4.peg.529 |
Organism-specific databases
EchoBASEi | EB3744 |
Phylogenomic databases
eggNOGi | COG0637, Bacteria |
HOGENOMi | CLU_045011_13_1_6 |
InParanoidi | P77247 |
PhylomeDBi | P77247 |
Enzyme and pathway databases
BioCyci | EcoCyc:G6932-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P77247 |
PROi | PR:P77247 |
Family and domain databases
Gene3Di | 1.10.150.240, 1 hit 3.40.50.1000, 1 hit |
InterProi | View protein in InterPro IPR036412, HAD-like_sf IPR006439, HAD-SF_hydro_IA IPR041492, HAD_2 IPR023214, HAD_sf IPR023198, PGP-like_dom2 |
Pfami | View protein in Pfam PF13419, HAD_2, 1 hit |
PRINTSi | PR00413, HADHALOGNASE |
SUPFAMi | SSF56784, SSF56784, 1 hit |
TIGRFAMsi | TIGR01509, HAD-SF-IA-v3, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | HXPB_ECOLI | |
Accessioni | P77247Primary (citable) accession number: P77247 Secondary accession number(s): P78167 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | February 1, 1997 | |
Last modified: | February 23, 2022 | |
This is version 158 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families