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UniProtKB - P77213 (GCS2_ECOLI)
Protein
Putative glutamate--cysteine ligase 2
Gene
ybdK
Organism
Escherichia coli (strain K12)
Status
Functioni
ATP-dependent carboxylate-amine ligase which exhibits weak glutamate--cysteine ligase activity. However, because of the low catalytic rate, the question remains whether L-cysteine is the actual biological substrate.
UniRule annotation1 PublicationMiscellaneous
Catalytic activity was tested towards all 20 common amino acids, but was only observed with L-cysteine.
Catalytic activityi
- EC:6.3.2.2UniRule annotation
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- glutamate-cysteine ligase activity Source: UniProtKB-EC
- ligase activity, forming carbon-nitrogen bonds Source: EcoCyc
GO - Biological processi
- cellular modified amino acid biosynthetic process Source: InterPro
Keywordsi
Molecular function | Ligase |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:G6326-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Putative glutamate--cysteine ligase 2UniRule annotation (EC:6.3.2.2UniRule annotation)Alternative name(s): Gamma-glutamylcysteine synthetase 2UniRule annotation Short name: GCS 2UniRule annotation Short name: Gamma-GCS 2UniRule annotation |
Gene namesi | Name:ybdK Ordered Locus Names:b0581, JW0570 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000218196 | 1 – 372 | Putative glutamate--cysteine ligase 2Add BLAST | 372 |
Proteomic databases
jPOSTi | P77213 |
PaxDbi | P77213 |
PRIDEi | P77213 |
Interactioni
Subunit structurei
Homodimer.
UniRule annotation1 PublicationProtein-protein interaction databases
BioGRIDi | 4260711, 13 interactors 851576, 1 interactor |
IntActi | P77213, 6 interactors |
STRINGi | 511145.b0581 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P77213 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P77213 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG2170, Bacteria |
HOGENOMi | CLU_044848_1_1_6 |
InParanoidi | P77213 |
PhylomeDBi | P77213 |
Family and domain databases
HAMAPi | MF_01609, Glu_cys_ligase_2, 1 hit |
InterProi | View protein in InterPro IPR006336, GCS2 IPR014746, Gln_synth/guanido_kin_cat_dom IPR011793, YbdK |
Pfami | View protein in Pfam PF04107, GCS2, 1 hit |
SUPFAMi | SSF55931, SSF55931, 1 hit |
TIGRFAMsi | TIGR02050, gshA_cyan_rel, 1 hit |
i Sequence
Sequence statusi: Complete.
P77213-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPLPDFHVSE PFTLGIELEM QVVNPPGYDL SQDSSMLIDA VKNKITAGEV
60 70 80 90 100
KHDITESMLE LATDVCRDIN QAAGQFSAMQ KVVLQAATDH HLEICGGGTH
110 120 130 140 150
PFQKWQRQEV CDNERYQRTL ENFGYLIQQA TVFGQHVHVG CASGDDAIYL
160 170 180 190 200
LHGLSRFVPH FIALSAASPY MQGTDTRFAS SRPNIFSAFP DNGPMPWVSN
210 220 230 240 250
WQQFEALFRC LSYTTMIDSI KDLHWDIRPS PHFGTVEVRV MDTPLTLSHA
260 270 280 290 300
VNMAGLIQAT AHWLLTERPF KHQEKDYLLY KFNRFQACRY GLEGVITDPH
310 320 330 340 350
TGDRRPLTED TLRLLEKIAP SAHKIGASSA IEALHRQVVS GLNEAQLMRD
360 370
FVADGGSLIG LVKKHCEIWA GD
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U82598 Genomic DNA Translation: AAB40779.1 U00096 Genomic DNA Translation: AAC73682.1 AP009048 Genomic DNA Translation: BAA35221.1 |
PIRi | C64791 |
RefSeqi | NP_415113.1, NC_000913.3 WP_001130654.1, NZ_SSZK01000104.1 |
Genome annotation databases
EnsemblBacteriai | AAC73682; AAC73682; b0581 BAA35221; BAA35221; BAA35221 |
GeneIDi | 947246 |
KEGGi | ecj:JW0570 eco:b0581 |
PATRICi | fig|1411691.4.peg.1693 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U82598 Genomic DNA Translation: AAB40779.1 U00096 Genomic DNA Translation: AAC73682.1 AP009048 Genomic DNA Translation: BAA35221.1 |
PIRi | C64791 |
RefSeqi | NP_415113.1, NC_000913.3 WP_001130654.1, NZ_SSZK01000104.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1R8G | X-ray | 2.15 | A/B | 1-372 | [»] | |
SMRi | P77213 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260711, 13 interactors 851576, 1 interactor |
IntActi | P77213, 6 interactors |
STRINGi | 511145.b0581 |
Proteomic databases
jPOSTi | P77213 |
PaxDbi | P77213 |
PRIDEi | P77213 |
Genome annotation databases
EnsemblBacteriai | AAC73682; AAC73682; b0581 BAA35221; BAA35221; BAA35221 |
GeneIDi | 947246 |
KEGGi | ecj:JW0570 eco:b0581 |
PATRICi | fig|1411691.4.peg.1693 |
Organism-specific databases
EchoBASEi | EB3408 |
Phylogenomic databases
eggNOGi | COG2170, Bacteria |
HOGENOMi | CLU_044848_1_1_6 |
InParanoidi | P77213 |
PhylomeDBi | P77213 |
Enzyme and pathway databases
BioCyci | EcoCyc:G6326-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P77213 |
PROi | PR:P77213 |
Family and domain databases
HAMAPi | MF_01609, Glu_cys_ligase_2, 1 hit |
InterProi | View protein in InterPro IPR006336, GCS2 IPR014746, Gln_synth/guanido_kin_cat_dom IPR011793, YbdK |
Pfami | View protein in Pfam PF04107, GCS2, 1 hit |
SUPFAMi | SSF55931, SSF55931, 1 hit |
TIGRFAMsi | TIGR02050, gshA_cyan_rel, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GCS2_ECOLI | |
Accessioni | P77213Primary (citable) accession number: P77213 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | February 1, 1997 | |
Last modified: | February 23, 2022 | |
This is version 145 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families