UniProtKB - P77182 (MNMC_ECOLI)
Protein
tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC
Gene
mnmC
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the last two steps in the biosynthesis of 5-methylaminomethyl-2-thiouridine (mnm5s2U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm5s2U34 to nm5s2U34, followed by the transfer of a methyl group from S-adenosyl-L-methionine to nm5s2U34, to form mnm5s2U34.2 Publications
Catalytic activityi
- 5-aminomethyl-2-thiouridine34 in tRNA + S-adenosyl-L-methionine = 5-methylaminomethyl-2-thiouridine34 in tRNA + H+ + S-adenosyl-L-homocysteineEC:2.1.1.61
Cofactori
FAD2 Publications
GO - Molecular functioni
- FAD binding Source: EcoCyc
- oxidoreductase activity Source: GO_Central
- oxidoreductase activity, acting on the CH-NH group of donors Source: InterPro
- tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase activity Source: EcoCyc
GO - Biological processi
- tRNA methylation Source: EcoCyc
- tRNA wobble uridine modification Source: EcoCyc
Keywordsi
Molecular function | Methyltransferase, Multifunctional enzyme, Oxidoreductase, Transferase |
Biological process | tRNA processing |
Ligand | FAD, Flavoprotein, S-adenosyl-L-methionine |
Enzyme and pathway databases
BioCyci | EcoCyc:G7199-MONOMER MetaCyc:G7199-MONOMER |
BRENDAi | 2.1.1.229, 2026 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:mnmC Synonyms:yfcK Ordered Locus Names:b2324, JW5380 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm Curated
Other locations
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 64 | E → A: Loss of methyltransferase activity. 1 Publication | 1 | |
Mutagenesisi | 178 | D → A: Strong decrease in methyltransferase activity. 1 Publication | 1 | |
Mutagenesisi | 180 | F → A: Strong decrease in methyltransferase activity. 1 Publication | 1 | |
Mutagenesisi | 271 | G → Q: 4-fold decrease in activity, but no change in FAD binding. 1 Publication | 1 | |
Mutagenesisi | 567 | R → A: Loss of activity, but no change in FAD binding. 1 Publication | 1 | |
Mutagenesisi | 618 | R → A: Loss of activity, but no change in FAD binding. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000095012 | 1 – 668 | tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmCAdd BLAST | 668 |
Proteomic databases
jPOSTi | P77182 |
PaxDbi | P77182 |
PRIDEi | P77182 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4261069, 20 interactors |
DIPi | DIP-28058N |
IntActi | P77182, 1 interactor |
STRINGi | 511145.b2324 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P77182 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 245 | tRNA (mnm(5)s(2)U34)-methyltransferaseAdd BLAST | 245 | |
Regioni | 270 – 668 | FAD-dependent cmnm(5)s(2)U34 oxidoreductaseAdd BLAST | 399 |
Sequence similaritiesi
In the N-terminal section; belongs to the methyltransferase superfamily. tRNA (mnm(5)s(2)U34)-methyltransferase family.Curated
In the C-terminal section; belongs to the DAO family.Curated
Phylogenomic databases
eggNOGi | COG0665, Bacteria COG4121, Bacteria |
HOGENOMi | CLU_022427_1_0_6 |
InParanoidi | P77182 |
PhylomeDBi | P77182 |
Family and domain databases
Gene3Di | 3.50.50.60, 1 hit |
HAMAPi | MF_01102, MnmC, 1 hit |
InterProi | View protein in InterPro IPR006076, FAD-dep_OxRdtase IPR036188, FAD/NAD-bd_sf IPR008471, MnmC-like_methylTransf IPR023032, tRNA_MAMT_biosynth_bifunc_MnmC IPR017610, tRNA_S-uridine_synth_MnmC_C |
Pfami | View protein in Pfam PF01266, DAO, 1 hit PF05430, Methyltransf_30, 1 hit |
SUPFAMi | SSF51905, SSF51905, 1 hit |
TIGRFAMsi | TIGR03197, MnmC_Cterm, 1 hit |
i Sequence
Sequence statusi: Complete.
P77182-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKHYSIQPAN LEFNAEGTPV SRDFDDVYFS NDNGLEETRY VFLGGNQLEV
60 70 80 90 100
RFPEHPHPLF VVAESGFGTG LNFLTLWQAF DQFREAHPQA QLQRLHFISF
110 120 130 140 150
EKFPLTRADL ALAHQHWPEL APWAEQLQAQ WPMPLPGCHR LLLDEGRVTL
160 170 180 190 200
DLWFGDINEL TSQLDDSLNQ KVDAWFLDGF APAKNPDMWT QNLFNAMARL
210 220 230 240 250
ARPGGTLATF TSAGFVRRGL QDAGFTMQKR KGFGRKREML CGVMEQTLPL
260 270 280 290 300
PCSAPWFNRT GSSKREAAII GGGIASALLS LALLRRGWQV TLYCADEAPA
310 320 330 340 350
LGASGNRQGA LYPLLSKHDE ALNRFFSNAF TFARRFYDQL PVKFDHDWCG
360 370 380 390 400
VTQLGWDEKS QHKIAQMLSM DLPAELAVAV EANAVEQITG VATNCSGITY
410 420 430 440 450
PQGGWLCPAE LTRNVLELAQ QQGLQIYYQY QLQNLSRKDD CWLLNFAGDQ
460 470 480 490 500
QATHSVVVLA NGHQISRFSQ TSTLPVYSVA GQVSHIPTTP ELAELKQVLC
510 520 530 540 550
YDGYLTPQNP ANQHHCIGAS YHRGSEDTAY SEDDQQQNRQ RLIDCFPQAQ
560 570 580 590 600
WAKEVDVSDK EARCGVRCAT RDHLPMVGNV PDYEATLVEY ASLAEQKDEA
610 620 630 640 650
VSAPVFDDLF MFAALGSRGL CSAPLCAEIL AAQMSDEPIP MDASTLAALN
660
PNRLWVRKLL KGKAVKAG
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC75384.2 AP009048 Genomic DNA Translation: BAA16181.2 |
PIRi | B65005 |
RefSeqi | NP_416827.4, NC_000913.3 WP_000683799.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC75384; AAC75384; b2324 BAA16181; BAA16181; BAA16181 |
GeneIDi | 946800 |
KEGGi | ecj:JW5380 eco:b2324 |
PATRICi | fig|511145.12.peg.2420 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC75384.2 AP009048 Genomic DNA Translation: BAA16181.2 |
PIRi | B65005 |
RefSeqi | NP_416827.4, NC_000913.3 WP_000683799.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3AWI | X-ray | 3.00 | A/B/C/D/E/F | 1-668 | [»] | |
SMRi | P77182 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261069, 20 interactors |
DIPi | DIP-28058N |
IntActi | P77182, 1 interactor |
STRINGi | 511145.b2324 |
Proteomic databases
jPOSTi | P77182 |
PaxDbi | P77182 |
PRIDEi | P77182 |
Genome annotation databases
EnsemblBacteriai | AAC75384; AAC75384; b2324 BAA16181; BAA16181; BAA16181 |
GeneIDi | 946800 |
KEGGi | ecj:JW5380 eco:b2324 |
PATRICi | fig|511145.12.peg.2420 |
Organism-specific databases
EchoBASEi | EB3867 |
Phylogenomic databases
eggNOGi | COG0665, Bacteria COG4121, Bacteria |
HOGENOMi | CLU_022427_1_0_6 |
InParanoidi | P77182 |
PhylomeDBi | P77182 |
Enzyme and pathway databases
BioCyci | EcoCyc:G7199-MONOMER MetaCyc:G7199-MONOMER |
BRENDAi | 2.1.1.229, 2026 |
Miscellaneous databases
PROi | PR:P77182 |
Family and domain databases
Gene3Di | 3.50.50.60, 1 hit |
HAMAPi | MF_01102, MnmC, 1 hit |
InterProi | View protein in InterPro IPR006076, FAD-dep_OxRdtase IPR036188, FAD/NAD-bd_sf IPR008471, MnmC-like_methylTransf IPR023032, tRNA_MAMT_biosynth_bifunc_MnmC IPR017610, tRNA_S-uridine_synth_MnmC_C |
Pfami | View protein in Pfam PF01266, DAO, 1 hit PF05430, Methyltransf_30, 1 hit |
SUPFAMi | SSF51905, SSF51905, 1 hit |
TIGRFAMsi | TIGR03197, MnmC_Cterm, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MNMC_ECOLI | |
Accessioni | P77182Primary (citable) accession number: P77182 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 27, 2002 |
Last sequence update: | March 27, 2002 | |
Last modified: | April 7, 2021 | |
This is version 153 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Uncharacterized protein families (UPF)
List of uncharacterized protein family (UPF) entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families