UniProtKB - P77173 (ZIPA_ECOLI)
Protein
Cell division protein ZipA
Gene
zipA
Organism
Escherichia coli (strain K12)
Status
Functioni
Essential cell division protein that stabilizes the FtsZ protofilaments by cross-linking them and that serves as a cytoplasmic membrane anchor for the Z ring (PubMed:9008158, PubMed:11847116, PubMed:22164258, PubMed:22304478, PubMed:23233671). Also required for the recruitment to the septal ring of the downstream cell division proteins FtsK, FtsQ, FtsL and FtsN (PubMed:11847116, PubMed:11948172). ZipA overproduction protects FtsZ from degradation by ClpP by preventing recognition by ClpX (PubMed:23233671). Does not affect the GTPase activity of FtsZ (PubMed:10209756).7 Publications
Miscellaneous
Has been isolated as a 91 kDa complex containing ZipA-EptA and an unidentified 24 kDa protein (PubMed:16079137), but it was shown later that there is no physical interaction between ZipA and EptA (PubMed:22304478).2 Publications
GO - Biological processi
- division septum assembly Source: EcoliWiki
- FtsZ-dependent cytokinesis Source: UniProtKB-UniRule
Keywordsi
Biological process | Cell cycle, Cell division |
Enzyme and pathway databases
BioCyci | EcoCyc:G7258-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Cell division protein ZipAUniRule annotationAlternative name(s): FtsZ interacting protein A1 Publication |
Gene namesi | Name:zipA1 PublicationUniRule annotation Ordered Locus Names:b2412, JW2404 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane 2 Publications; Single-pass type I membrane protein 1 Publication
Note: Localizes to the Z ring in an FtsZ-dependent manner (PubMed:9008158, PubMed:9864327, PubMed:10209756). Localization does not depend upon FtsA or FtsI (PubMed:9864327, PubMed:10209756).3 Publications
Plasma Membrane
- integral component of plasma membrane Source: EcoliWiki
- plasma membrane Source: EcoCyc
Other locations
- cell division site Source: EcoliWiki
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 6 | PeriplasmicUniRule annotation1 Publication | 6 | |
Transmembranei | 7 – 27 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 28 – 328 | CytoplasmicUniRule annotation1 PublicationAdd BLAST | 301 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Chemistry databases
ChEMBLi | CHEMBL3954 |
DrugBanki | DB02191, (7as,12ar,12bs)-1,2,3,4,7a,12,12a,12b-Octahydroindolo[2,3-a]Quinolizin-7(6h)-One DB03916, 4-{2-[4-(2-Aminoethyl)Piperazin-1-Yl]Pyridin-4-Yl}-N-(3-Chloro-4-Methylphenyl)Pyrimidin-2-Amine DB04154, N-Methyl-N-[3-(6-Phenyl[1,2,4]Triazolo[4,3-B]Pyridazin-3-Yl)Phenyl]Acetamide DB01967, N-{3-[(7ar,12as,12bs)-7-Oxo-1,3,4,6,7,7a,12a,12b-Octahydroindolo[2,3-a]Quinolizin-12(2h)-Yl]Propyl}Propane-2-Sulfonamide |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000214521 | 1 – 328 | Cell division protein ZipAAdd BLAST | 328 |
Proteomic databases
jPOSTi | P77173 |
PaxDbi | P77173 |
PRIDEi | P77173 |
Expressioni
Inductioni
Repressed 1.5-fold by hydroxyurea.1 Publication
Interactioni
Subunit structurei
Binary interactionsi
P77173
With | #Exp. | IntAct |
---|---|---|
ftsZ [P0A9A6] | 5 | EBI-1029213,EBI-370963 |
Protein-protein interaction databases
BioGRIDi | 4261708, 561 interactors 851209, 1 interactor |
ComplexPortali | CPX-1936, Divisome complex |
DIPi | DIP-1157N |
IntActi | P77173, 2 interactors |
STRINGi | 511145.b2412 |
Chemistry databases
BindingDBi | P77173 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P77173 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P77173 |
Family & Domainsi
Domaini
Contains an N-terminal transmembrane domain, followed by a charged domain, an unstructured P/Q domain rich in proline and glutamine, and a C-terminal FtsZ-binding domain.1 Publication
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG3115, Bacteria |
HOGENOMi | CLU_030174_1_0_6 |
InParanoidi | P77173 |
Family and domain databases
CDDi | cd00231, ZipA, 1 hit |
DisProti | DP00161 |
Gene3Di | 3.30.1400.10, 1 hit |
HAMAPi | MF_00509, ZipA, 1 hit |
InterProi | View protein in InterPro IPR011919, Cell_div_ZipA IPR007449, ZipA_FtsZ-bd_C IPR036765, ZipA_FtsZ-bd_C_sf |
PANTHERi | PTHR38685, PTHR38685, 1 hit |
Pfami | View protein in Pfam PF04354, ZipA_C, 1 hit |
SMARTi | View protein in SMART SM00771, ZipA_C, 1 hit |
SUPFAMi | SSF64383, SSF64383, 1 hit |
TIGRFAMsi | TIGR02205, septum_zipA, 1 hit |
i Sequence
Sequence statusi: Complete.
P77173-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MMQDLRLILI IVGAIAIIAL LVHGFWTSRK ERSSMFRDRP LKRMKSKRDD
60 70 80 90 100
DSYDEDVEDD EGVGEVRVHR VNHAPANAQE HEAARPSPQH QYQPPYASAQ
110 120 130 140 150
PRQPVQQPPE AQVPPQHAPH PAQPVQQPAY QPQPEQPLQQ PVSPQVAPAP
160 170 180 190 200
QPVHSAPQPA QQAFQPAEPV AAPQPEPVAE PAPVMDKPKR KEAVIIMNVA
210 220 230 240 250
AHHGSELNGE LLLNSIQQAG FIFGDMNIYH RHLSPDGSGP ALFSLANMVK
260 270 280 290 300
PGTFDPEMKD FTTPGVTIFM QVPSYGDELQ NFKLMLQSAQ HIADEVGGVV
310 320
LDDQRRMMTP QKLREYQDII REVKDANA
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 211 | L → A in AAB42061 (PubMed:9008158).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U74650 Genomic DNA Translation: AAB42061.1 U00096 Genomic DNA Translation: AAC75465.1 AP009048 Genomic DNA Translation: BAA16284.1 |
PIRi | C65015 |
RefSeqi | NP_416907.1, NC_000913.3 WP_001300494.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC75465; AAC75465; b2412 BAA16284; BAA16284; BAA16284 |
GeneIDi | 57729417 946869 |
KEGGi | ecj:JW2404 eco:b2412 |
PATRICi | fig|511145.12.peg.2506 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U74650 Genomic DNA Translation: AAB42061.1 U00096 Genomic DNA Translation: AAC75465.1 AP009048 Genomic DNA Translation: BAA16284.1 |
PIRi | C65015 |
RefSeqi | NP_416907.1, NC_000913.3 WP_001300494.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1F46 | X-ray | 1.50 | A/B | 189-328 | [»] | |
1F47 | X-ray | 1.95 | B | 185-328 | [»] | |
1F7W | NMR | - | A | 185-328 | [»] | |
1F7X | NMR | - | A | 185-328 | [»] | |
1S1J | X-ray | 2.18 | A/B | 185-328 | [»] | |
1S1S | X-ray | 2.10 | A/B | 185-328 | [»] | |
1Y2F | X-ray | 2.00 | A | 190-328 | [»] | |
1Y2G | X-ray | 1.90 | A/B | 189-328 | [»] | |
SMRi | P77173 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261708, 561 interactors 851209, 1 interactor |
ComplexPortali | CPX-1936, Divisome complex |
DIPi | DIP-1157N |
IntActi | P77173, 2 interactors |
STRINGi | 511145.b2412 |
Chemistry databases
BindingDBi | P77173 |
ChEMBLi | CHEMBL3954 |
DrugBanki | DB02191, (7as,12ar,12bs)-1,2,3,4,7a,12,12a,12b-Octahydroindolo[2,3-a]Quinolizin-7(6h)-One DB03916, 4-{2-[4-(2-Aminoethyl)Piperazin-1-Yl]Pyridin-4-Yl}-N-(3-Chloro-4-Methylphenyl)Pyrimidin-2-Amine DB04154, N-Methyl-N-[3-(6-Phenyl[1,2,4]Triazolo[4,3-B]Pyridazin-3-Yl)Phenyl]Acetamide DB01967, N-{3-[(7ar,12as,12bs)-7-Oxo-1,3,4,6,7,7a,12a,12b-Octahydroindolo[2,3-a]Quinolizin-12(2h)-Yl]Propyl}Propane-2-Sulfonamide |
Proteomic databases
jPOSTi | P77173 |
PaxDbi | P77173 |
PRIDEi | P77173 |
Genome annotation databases
EnsemblBacteriai | AAC75465; AAC75465; b2412 BAA16284; BAA16284; BAA16284 |
GeneIDi | 57729417 946869 |
KEGGi | ecj:JW2404 eco:b2412 |
PATRICi | fig|511145.12.peg.2506 |
Organism-specific databases
EchoBASEi | EB3921 |
Phylogenomic databases
eggNOGi | COG3115, Bacteria |
HOGENOMi | CLU_030174_1_0_6 |
InParanoidi | P77173 |
Enzyme and pathway databases
BioCyci | EcoCyc:G7258-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P77173 |
PROi | PR:P77173 |
Family and domain databases
CDDi | cd00231, ZipA, 1 hit |
DisProti | DP00161 |
Gene3Di | 3.30.1400.10, 1 hit |
HAMAPi | MF_00509, ZipA, 1 hit |
InterProi | View protein in InterPro IPR011919, Cell_div_ZipA IPR007449, ZipA_FtsZ-bd_C IPR036765, ZipA_FtsZ-bd_C_sf |
PANTHERi | PTHR38685, PTHR38685, 1 hit |
Pfami | View protein in Pfam PF04354, ZipA_C, 1 hit |
SMARTi | View protein in SMART SM00771, ZipA_C, 1 hit |
SUPFAMi | SSF64383, SSF64383, 1 hit |
TIGRFAMsi | TIGR02205, septum_zipA, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ZIPA_ECOLI | |
Accessioni | P77173Primary (citable) accession number: P77173 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | August 29, 2003 | |
Last modified: | April 7, 2021 | |
This is version 160 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families