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Protein

Aldehyde oxidoreductase iron-sulfur-binding subunit PaoA

Gene

paoA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Oxidizes aldehydes to the corresponding carboxylic acids with a preference for aromatic aldehydes. It might play a role in the detoxification of aldehydes to avoid cell damage.1 Publication

Catalytic activityi

An aldehyde + acceptor + H2O = a carboxylate + reduced acceptor.2 Publications

Cofactori

[2Fe-2S] cluster2 PublicationsNote: Binds 2 [2Fe-2S] clusters.1 Publication1 Publication

Activity regulationi

The complex requires PaoD for activity.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi99Iron-sulfur (2Fe-2S) 1Combined sources1 Publication1
Metal bindingi104Iron-sulfur (2Fe-2S) 1Combined sources1 Publication1
Metal bindingi105Iron-sulfur (2Fe-2S) 1; via amide nitrogenCombined sources1 Publication1
Metal bindingi107Iron-sulfur (2Fe-2S) 1Combined sources1 Publication1
Metal bindingi119Iron-sulfur (2Fe-2S) 1Combined sources1 Publication1
Metal bindingi158Iron-sulfur (2Fe-2S) 2Combined sources1 Publication1
Metal bindingi161Iron-sulfur (2Fe-2S) 2Combined sources1 Publication1
Metal bindingi208Iron-sulfur (2Fe-2S) 2Combined sources1 Publication1
Metal bindingi210Iron-sulfur (2Fe-2S) 2Combined sources1 Publication1

GO - Molecular functioni

Keywordsi

Molecular functionOxidoreductase
Ligand2Fe-2S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:G6157-MONOMER
MetaCyc:G6157-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Aldehyde oxidoreductase iron-sulfur-binding subunit PaoACurated (EC:1.2.99.62 Publications)
Gene namesi
Name:paoA1 Publication
Synonyms:yagT
Ordered Locus Names:b0286, JW0280
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13559 yagT

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Periplasm

Pathology & Biotechi

Disruption phenotypei

Mutation results in complete impairment of cell growth in the presence of cinnamaldehyde.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 53Tat-type signalPROSITE-ProRule annotationAdd BLAST53
ChainiPRO_000018941454 – 229Aldehyde oxidoreductase iron-sulfur-binding subunit PaoAAdd BLAST176

Post-translational modificationi

Exported by the Tat system (PubMed:17218314). The position of the signal peptide cleavage has not been experimentally proven (Probable).Curated1 Publication

Proteomic databases

PaxDbiP77165
PRIDEiP77165

Interactioni

Subunit structurei

Heterotrimer composed of PaoA, PaoB and PaoC.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
proXP0AFM22EBI-1115563,EBI-1129961

Protein-protein interaction databases

BioGridi4259786, 3 interactors
IntActiP77165, 10 interactors
STRINGi316385.ECDH10B_0274

Structurei

Secondary structure

1229
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP77165
SMRiP77165
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini61 – 1372Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd BLAST77

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107XKX Bacteria
COG2080 LUCA
HOGENOMiHOG000166647
InParanoidiP77165
KOiK13483
PhylomeDBiP77165

Family and domain databases

CDDicd00207 fer2, 1 hit
Gene3Di3.10.20.30, 1 hit
InterProiView protein in InterPro
IPR002888 2Fe-2S-bd
IPR036884 2Fe-2S-bd_dom_sf
IPR036010 2Fe-2S_ferredoxin-like_sf
IPR001041 2Fe-2S_ferredoxin-type
IPR006058 2Fe2S_fd_BS
IPR012675 Beta-grasp_dom_sf
IPR006311 TAT_signal
PfamiView protein in Pfam
PF00111 Fer2, 1 hit
PF01799 Fer2_2, 1 hit
SUPFAMiSSF47741 SSF47741, 1 hit
SSF54292 SSF54292, 1 hit
PROSITEiView protein in PROSITE
PS00197 2FE2S_FER_1, 1 hit
PS51085 2FE2S_FER_2, 1 hit
PS51318 TAT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P77165-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSNQGEYPED NRVGKHEPHD LSLTRRDLIK VSAATAATAV VYPHSTLAAS
60 70 80 90 100
VPAATPAPEI MPLTLKVNGK TEQLEVDTRT TLLDTLRENL HLIGTKKGCD
110 120 130 140 150
HGQCGACTVL VNGRRLNACL TLAVMHQGAE ITTIEGLGSP DNLHPMQAAF
160 170 180 190 200
IKHDGFQCGY CTSGQICSSV AVLKEIQDGI PSHVTVDLVS APETTADEIR
210 220
ERMSGNICRC GAYANILAAI EDAAGEIKS
Length:229
Mass (Da):24,343
Last modified:February 1, 1997 - v1
Checksum:i216961D7BD21836C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73857 Genomic DNA Translation: AAB18015.1
U00096 Genomic DNA Translation: AAC73389.1
AP009048 Genomic DNA Translation: BAE76070.1
PIRiF64754
RefSeqiNP_414820.1, NC_000913.3
WP_000070700.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73389; AAC73389; b0286
BAE76070; BAE76070; BAE76070
GeneIDi945330
KEGGiecj:JW0280
eco:b0286
PATRICifig|1411691.4.peg.1992

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73857 Genomic DNA Translation: AAB18015.1
U00096 Genomic DNA Translation: AAC73389.1
AP009048 Genomic DNA Translation: BAE76070.1
PIRiF64754
RefSeqiNP_414820.1, NC_000913.3
WP_000070700.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5G5GX-ray1.70A1-229[»]
5G5HX-ray2.30A1-229[»]
ProteinModelPortaliP77165
SMRiP77165
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259786, 3 interactors
IntActiP77165, 10 interactors
STRINGi316385.ECDH10B_0274

Proteomic databases

PaxDbiP77165
PRIDEiP77165

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73389; AAC73389; b0286
BAE76070; BAE76070; BAE76070
GeneIDi945330
KEGGiecj:JW0280
eco:b0286
PATRICifig|1411691.4.peg.1992

Organism-specific databases

EchoBASEiEB3329
EcoGeneiEG13559 yagT

Phylogenomic databases

eggNOGiENOG4107XKX Bacteria
COG2080 LUCA
HOGENOMiHOG000166647
InParanoidiP77165
KOiK13483
PhylomeDBiP77165

Enzyme and pathway databases

BioCyciEcoCyc:G6157-MONOMER
MetaCyc:G6157-MONOMER

Miscellaneous databases

PROiPR:P77165

Family and domain databases

CDDicd00207 fer2, 1 hit
Gene3Di3.10.20.30, 1 hit
InterProiView protein in InterPro
IPR002888 2Fe-2S-bd
IPR036884 2Fe-2S-bd_dom_sf
IPR036010 2Fe-2S_ferredoxin-like_sf
IPR001041 2Fe-2S_ferredoxin-type
IPR006058 2Fe2S_fd_BS
IPR012675 Beta-grasp_dom_sf
IPR006311 TAT_signal
PfamiView protein in Pfam
PF00111 Fer2, 1 hit
PF01799 Fer2_2, 1 hit
SUPFAMiSSF47741 SSF47741, 1 hit
SSF54292 SSF54292, 1 hit
PROSITEiView protein in PROSITE
PS00197 2FE2S_FER_1, 1 hit
PS51085 2FE2S_FER_2, 1 hit
PS51318 TAT, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPAOA_ECOLI
AccessioniPrimary (citable) accession number: P77165
Secondary accession number(s): Q2MCD6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: November 7, 2018
This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
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Main funding by: National Institutes of Health

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