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Entry version 138 (02 Dec 2020)
Sequence version 1 (01 Feb 1997)
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Protein

Kojibiose phosphorylase

Gene

ycjT

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

In vitro catalyzes the phosphorolysis of D-kojibiose into beta-D-glucose 1-phosphate and D-glucose. No other disaccharides tested substitute for D-kojibiose. In the reverse direction disaccharides can be formed from beta-D-glucose 1-phosphate plus D-glucose, L-sorbose, D-sorbitol, L-iditol or 1,5-anhydro-D-glucitol, but with low efficiency. The beta-D-glucose 1-phosphate product is the substrate for YcjU (AC P77366), the next apparent enzyme in the putative biochemical pathway encoded in this locus (yjcM to ycjW).1 Publication

Miscellaneous

Kojibiose does not support bacterial growth of K12 / BW25113 nor of BL-21(DE3), perhaps because it is not transported into E.coli.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

kcat is 1.0 sec(-1) for phosphorolysis of kojibiose, and kcat is 0.8 sec(-1) for the reverse reaction.1 Publication
  1. KM=1.05 mM for kojibiose (at pH 7.5, 30 degrees Celsius)1 Publication
  2. KM=3.0 mM for phosphate (at pH 7.5, 30 degrees Celsius)1 Publication
  3. KM=1.7 mM for D-glucose (at pH 7.5, 30 degrees Celsius)1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei473Proton donorBy similarity1

    <p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

    GO - Biological processi

    <p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

    Molecular functionGlycosidase, Hydrolase, Transferase

    Enzyme and pathway databases

    BioCyc Collection of Pathway/Genome Databases

    More...
    BioCyci
    EcoCyc:G6654-MONOMER
    MetaCyc:G6654-MONOMER

    Protein family/group databases

    Carbohydrate-Active enZymes

    More...
    CAZyi
    GH65, Glycoside Hydrolase Family 65

    <p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
    Recommended name:
    Kojibiose phosphorylase1 Publication (EC:2.4.1.2301 Publication)
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
    Name:ycjT
    Ordered Locus Names:b1316, JW1309
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
    • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    <p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

    <p>This subsection of the 'Pathology and Biotech' section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

    No visible phenotype when grown on glucose.1 Publication

    <p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001080171 – 755Kojibiose phosphorylaseAdd BLAST755

    Proteomic databases

    PaxDb, a database of protein abundance averages across all three domains of life

    More...
    PaxDbi
    P77154

    PRoteomics IDEntifications database

    More...
    PRIDEi
    P77154

    <p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

    Protein-protein interaction databases

    The Biological General Repository for Interaction Datasets (BioGRID)

    More...
    BioGRIDi
    4263194, 19 interactors

    Protein interaction database and analysis system

    More...
    IntActi
    P77154, 1 interactor

    STRING: functional protein association networks

    More...
    STRINGi
    511145.b1316

    <p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

    3D structure databases

    SWISS-MODEL Repository - a database of annotated 3D protein structure models

    More...
    SMRi
    P77154

    Database of comparative protein structure models

    More...
    ModBasei
    Search...

    <p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni333 – 334Substrate bindingBy similarity2
    Regioni573 – 574Substrate bindingBy similarity2

    <p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

    Belongs to the glycosyl hydrolase 65 family.Curated

    Phylogenomic databases

    evolutionary genealogy of genes: Non-supervised Orthologous Groups

    More...
    eggNOGi
    COG1554, Bacteria

    The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

    More...
    HOGENOMi
    CLU_006285_2_1_6

    InParanoid: Eukaryotic Ortholog Groups

    More...
    InParanoidi
    P77154

    Database for complete collections of gene phylogenies

    More...
    PhylomeDBi
    P77154

    Family and domain databases

    Gene3D Structural and Functional Annotation of Protein Families

    More...
    Gene3Di
    1.50.10.10, 1 hit
    2.70.98.40, 1 hit

    Integrated resource of protein families, domains and functional sites

    More...
    InterProi
    View protein in InterPro
    IPR008928, 6-hairpin_glycosidase_sf
    IPR012341, 6hp_glycosidase-like_sf
    IPR011013, Gal_mutarotase_sf_dom
    IPR005194, Glyco_hydro_65_C
    IPR005195, Glyco_hydro_65_M
    IPR005196, Glyco_hydro_65_N
    IPR037018, Glyco_hydro_65_N_sf
    IPR017045, Malt_Pase/Glycosyl_Hdrlase

    Pfam protein domain database

    More...
    Pfami
    View protein in Pfam
    PF03633, Glyco_hydro_65C, 1 hit
    PF03632, Glyco_hydro_65m, 1 hit
    PF03636, Glyco_hydro_65N, 1 hit

    PIRSF; a whole-protein classification database

    More...
    PIRSFi
    PIRSF036289, Glycosyl_hydrolase_malt_phosph, 1 hit

    Superfamily database of structural and functional annotation

    More...
    SUPFAMi
    SSF48208, SSF48208, 1 hit
    SSF74650, SSF74650, 1 hit

    <p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

    <p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

    P77154-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MTRPVTLSEP HFSQHTLNKY ASLMAQGNGY LGLRASHEED YTRQTRGMYL
    60 70 80 90 100
    AGLYHRAGKG EINELVNLPD VVGMEIAING EVFSLSHEAW QRELDFASGE
    110 120 130 140 150
    LRRNVVWRTS NGSGYTIASR RFVSADQLPL IALEITITPL DADASVLIST
    160 170 180 190 200
    GIDATQTNHG RQHLDETQVR VFGQHLMQGS YTTQDGRSDV AISCCCKVSG
    210 220 230 240 250
    DVQQCYTAKE RRLLQHTSAQ LHAGETMTLQ KLVWIDWRDD RQAALDEWGS
    260 270 280 290 300
    ASLRQLEMCA QQSYDQLLAA STENWRQWWQ KRRITVNGGE AHDQQALDYA
    310 320 330 340 350
    LYHLRIMTPA HDERSSIAAK GLTGEGYKGH VFWDTEVFLL PFHLFSDPTV
    360 370 380 390 400
    ARSLLRYRWH NLPGAQEKAR RNGWQGALFP WESARSGEEE TPEFAAINIR
    410 420 430 440 450
    TGLRQKVASA QAEHHLVADI AWAVIQYWQT TGDESFIAHE GMALLLETAK
    460 470 480 490 500
    FWISRAVRVN DRLEIHDVIG PDEYTEHVNN NAYTSYMARY NVQQALNIAR
    510 520 530 540 550
    QFGCSDDAFI HRAEMFLKEL WMPEIQPDGV LPQDDSFMAK PAINLAKYKA
    560 570 580 590 600
    AAGKQTILLD YSRAEVNEMQ ILKQADVVML NYMLPEQFSA ASCLANLQFY
    610 620 630 640 650
    EPRTIHDSSL SKAIHGIVAA RCGLLTQSYQ FWREGTEIDL GADPHSCDDG
    660 670 680 690 700
    IHAAATGAIW LGAIQGFAGV SVRDGELHLN PALPEQWQQL SFPLFWQGCE
    710 720 730 740 750
    LQVTLDAQRI AIRTSAPVSL RLNGQLITVA EESVFCLGDF ILPFNGTATK

    HQEDE
    Length:755
    Mass (Da):84,867
    Last modified:February 1, 1997 - v1
    <p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i89A645A7A5AB599D
    GO

    Sequence databases

    Select the link destinations:

    EMBL nucleotide sequence database

    More...
    EMBLi

    GenBank nucleotide sequence database

    More...
    GenBanki

    DNA Data Bank of Japan; a nucleotide sequence database

    More...
    DDBJi
    Links Updated
    U00096 Genomic DNA Translation: AAC74398.1
    AP009048 Genomic DNA Translation: BAA14891.1

    Protein sequence database of the Protein Information Resource

    More...
    PIRi
    G64880

    NCBI Reference Sequences

    More...
    RefSeqi
    NP_415832.1, NC_000913.3
    WP_000198036.1, NZ_SSZK01000012.1

    Genome annotation databases

    Ensembl bacterial and archaeal genome annotation project

    More...
    EnsemblBacteriai
    AAC74398; AAC74398; b1316
    BAA14891; BAA14891; BAA14891

    Database of genes from NCBI RefSeq genomes

    More...
    GeneIDi
    945895

    KEGG: Kyoto Encyclopedia of Genes and Genomes

    More...
    KEGGi
    ecj:JW1309
    eco:b1316

    Pathosystems Resource Integration Center (PATRIC)

    More...
    PATRICi
    fig|1411691.4.peg.963

    <p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

    <p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U00096 Genomic DNA Translation: AAC74398.1
    AP009048 Genomic DNA Translation: BAA14891.1
    PIRiG64880
    RefSeqiNP_415832.1, NC_000913.3
    WP_000198036.1, NZ_SSZK01000012.1

    3D structure databases

    SMRiP77154
    ModBaseiSearch...

    Protein-protein interaction databases

    BioGRIDi4263194, 19 interactors
    IntActiP77154, 1 interactor
    STRINGi511145.b1316

    Protein family/group databases

    CAZyiGH65, Glycoside Hydrolase Family 65

    Proteomic databases

    PaxDbiP77154
    PRIDEiP77154

    Genome annotation databases

    EnsemblBacteriaiAAC74398; AAC74398; b1316
    BAA14891; BAA14891; BAA14891
    GeneIDi945895
    KEGGiecj:JW1309
    eco:b1316
    PATRICifig|1411691.4.peg.963

    Organism-specific databases

    EchoBASE - an integrated post-genomic database for E. coli

    More...
    EchoBASEi
    EB3676

    Phylogenomic databases

    eggNOGiCOG1554, Bacteria
    HOGENOMiCLU_006285_2_1_6
    InParanoidiP77154
    PhylomeDBiP77154

    Enzyme and pathway databases

    BioCyciEcoCyc:G6654-MONOMER
    MetaCyc:G6654-MONOMER

    Miscellaneous databases

    Protein Ontology

    More...
    PROi
    PR:P77154

    Family and domain databases

    Gene3Di1.50.10.10, 1 hit
    2.70.98.40, 1 hit
    InterProiView protein in InterPro
    IPR008928, 6-hairpin_glycosidase_sf
    IPR012341, 6hp_glycosidase-like_sf
    IPR011013, Gal_mutarotase_sf_dom
    IPR005194, Glyco_hydro_65_C
    IPR005195, Glyco_hydro_65_M
    IPR005196, Glyco_hydro_65_N
    IPR037018, Glyco_hydro_65_N_sf
    IPR017045, Malt_Pase/Glycosyl_Hdrlase
    PfamiView protein in Pfam
    PF03633, Glyco_hydro_65C, 1 hit
    PF03632, Glyco_hydro_65m, 1 hit
    PF03636, Glyco_hydro_65N, 1 hit
    PIRSFiPIRSF036289, Glycosyl_hydrolase_malt_phosph, 1 hit
    SUPFAMiSSF48208, SSF48208, 1 hit
    SSF74650, SSF74650, 1 hit

    ProtoNet; Automatic hierarchical classification of proteins

    More...
    ProtoNeti
    Search...

    MobiDB: a database of protein disorder and mobility annotations

    More...
    MobiDBi
    Search...

    <p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

    <p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiKOJP_ECOLI
    <p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P77154
    <p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: February 1, 1997
    Last modified: December 2, 2020
    This is version 138 of the entry and version 1 of the sequence. See complete history.
    <p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    <p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

    Keywords - Technical termi

    Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families
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