UniProtKB - P76641 (GUAD_ECOLI)
Protein
Guanine deaminase
Gene
guaD
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia.
Catalytic activityi
- EC:3.5.4.3
Cofactori
Zn2+Note: Binds 1 zinc ion per subunit.
: guanine degradation Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes xanthine from guanine.Proteins known to be involved in this subpathway in this organism are:
- Guanine deaminase (guaD), Guanine deaminase (guaD)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes xanthine from guanine, the pathway guanine degradation and in Purine metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 82 | Zinc; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 84 | Zinc; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 237 | Zinc; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 327 | ZincBy similarity | 1 | |
Binding sitei | 327 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- ammeline aminohydrolase activity Source: EcoCyc
- deaminase activity Source: GO_Central
- guanine deaminase activity Source: EcoCyc
- zinc ion binding Source: EcoCyc
GO - Biological processi
- guanine catabolic process Source: UniProtKB-UniPathway
- guanine metabolic process Source: GO_Central
Keywordsi
Molecular function | Hydrolase |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
BioCyci | EcoCyc:G7502-MONOMER MetaCyc:G7502-MONOMER |
UniPathwayi | UPA00603;UER00660 |
Names & Taxonomyi
Protein namesi | Recommended name: Guanine deaminase (EC:3.5.4.3)Short name: Guanase Short name: Guanine aminase Alternative name(s): Guanine aminohydrolase Short name: GAH |
Gene namesi | Name:guaD Synonyms:ygfP Ordered Locus Names:b2883, JW5466 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000122297 | 1 – 439 | Guanine deaminaseAdd BLAST | 439 |
Proteomic databases
jPOSTi | P76641 |
PaxDbi | P76641 |
PRIDEi | P76641 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4259227, 10 interactors 851689, 1 interactor |
STRINGi | 511145.b2883 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P76641 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 84 – 87 | Substrate bindingBy similarity | 4 | |
Regioni | 209 – 210 | Substrate bindingBy similarity | 2 | |
Regioni | 237 – 240 | Substrate bindingBy similarity | 4 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0402, Bacteria |
HOGENOMi | CLU_012358_0_2_6 |
InParanoidi | P76641 |
PhylomeDBi | P76641 |
Family and domain databases
CDDi | cd01303, GDEase, 1 hit |
Gene3Di | 2.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR006680, Amidohydro-rel IPR014311, Guanine_deaminase IPR011059, Metal-dep_hydrolase_composite IPR032466, Metal_Hydrolase |
PANTHERi | PTHR11271:SF6, PTHR11271:SF6, 1 hit |
Pfami | View protein in Pfam PF01979, Amidohydro_1, 1 hit |
SUPFAMi | SSF51338, SSF51338, 1 hit SSF51556, SSF51556, 1 hit |
TIGRFAMsi | TIGR02967, guan_deamin, 1 hit |
i Sequence
Sequence statusi: Complete.
P76641-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MMSGEHTLKA VRGSFIDVTR TIDNPEEIAS ALRFIEDGLL LIKQGKVEWF
60 70 80 90 100
GEWENGKHQI PDTIRVRDYR GKLIVPGFVD THIHYPQSEM VGAYGEQLLE
110 120 130 140 150
WLNKHTFPTE RRYEDLEYAR EMSAFFIKQL LRNGTTTALV FGTVHPQSVD
160 170 180 190 200
ALFEAASHIN MRMIAGKVMM DRNAPDYLLD TAESSYHQSK ELIERWHKNG
210 220 230 240 250
RLLYAITPRF APTSSPEQMA MAQRLKEEYP DTWVHTHLCE NKDEIAWVKS
260 270 280 290 300
LYPDHDGYLD VYHQYGLTGK NCVFAHCVHL EEKEWDRLSE TKSSIAFCPT
310 320 330 340 350
SNLYLGSGLF NLKKAWQKKV KVGMGTDIGA GTTFNMLQTL NEAYKVLQLQ
360 370 380 390 400
GYRLSAYEAF YLATLGGAKS LGLDDLIGNF LPGKEADFVV MEPTATPLQQ
410 420 430
LRYDNSVSLV DKLFVMMTLG DDRSIYRTYV DGRLVYERN
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U28375 Genomic DNA Translation: AAA83064.1 U00096 Genomic DNA Translation: AAC75921.1 AP009048 Genomic DNA Translation: BAE76949.1 |
PIRi | C65072 |
RefSeqi | NP_417359.1, NC_000913.3 |
Genome annotation databases
EnsemblBacteriai | AAC75921; AAC75921; b2883 BAE76949; BAE76949; BAE76949 |
GeneIDi | 947366 |
KEGGi | ecj:JW5466 eco:b2883 |
PATRICi | fig|511145.12.peg.2976 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U28375 Genomic DNA Translation: AAA83064.1 U00096 Genomic DNA Translation: AAC75921.1 AP009048 Genomic DNA Translation: BAE76949.1 |
PIRi | C65072 |
RefSeqi | NP_417359.1, NC_000913.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6OHB | X-ray | 2.30 | A/B/C/D | 1-439 | [»] | |
6OHC | X-ray | 2.30 | A/B/C/D | 1-439 | [»] | |
SMRi | P76641 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259227, 10 interactors 851689, 1 interactor |
STRINGi | 511145.b2883 |
Proteomic databases
jPOSTi | P76641 |
PaxDbi | P76641 |
PRIDEi | P76641 |
Genome annotation databases
EnsemblBacteriai | AAC75921; AAC75921; b2883 BAE76949; BAE76949; BAE76949 |
GeneIDi | 947366 |
KEGGi | ecj:JW5466 eco:b2883 |
PATRICi | fig|511145.12.peg.2976 |
Organism-specific databases
EchoBASEi | EB2878 |
Phylogenomic databases
eggNOGi | COG0402, Bacteria |
HOGENOMi | CLU_012358_0_2_6 |
InParanoidi | P76641 |
PhylomeDBi | P76641 |
Enzyme and pathway databases
UniPathwayi | UPA00603;UER00660 |
BioCyci | EcoCyc:G7502-MONOMER MetaCyc:G7502-MONOMER |
Miscellaneous databases
PROi | PR:P76641 |
Family and domain databases
CDDi | cd01303, GDEase, 1 hit |
Gene3Di | 2.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR006680, Amidohydro-rel IPR014311, Guanine_deaminase IPR011059, Metal-dep_hydrolase_composite IPR032466, Metal_Hydrolase |
PANTHERi | PTHR11271:SF6, PTHR11271:SF6, 1 hit |
Pfami | View protein in Pfam PF01979, Amidohydro_1, 1 hit |
SUPFAMi | SSF51338, SSF51338, 1 hit SSF51556, SSF51556, 1 hit |
TIGRFAMsi | TIGR02967, guan_deamin, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GUAD_ECOLI | |
Accessioni | P76641Primary (citable) accession number: P76641 Secondary accession number(s): Q2M9V7, Q46816 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | February 1, 1997 | |
Last modified: | April 7, 2021 | |
This is version 139 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families