UniProtKB - P76578 (A2MG_ECOLI)
Protein
Alpha-2-macroglobulin
Gene
yfhM
Organism
Escherichia coli (strain K12)
Status
Functioni
Protects the bacterial cell from host peptidases (PubMed:18697741, PubMed:26143919, PubMed:26100869). Acts by a 'trapping' mechanism. Cleavage of the bait-region domain by host peptidases leads to a global conformational change, which results in entrapment of the host peptidase and activation of the thioester bond that covalently binds the attacking host peptidase (PubMed:26143919, PubMed:26100869). Trapped peptidases are still active except against very large substrates (PubMed:26100869). May protect the entire periplam, including the lipoproteins anchored to the periplasmic side of the outer membrane, against intruding endopeptidases (PubMed:26100869).3 Publications
Miscellaneous
Bacterial alpha-2-macroglobulins were probably acquired one or more times by horizontal gene transfer from metazoan hosts.1 Publication
GO - Molecular functioni
- endopeptidase inhibitor activity Source: EcoCyc
GO - Biological processi
- negative regulation of endopeptidase activity Source: EcoCyc
Keywordsi
Molecular function | Protease inhibitor |
Enzyme and pathway databases
BioCyci | EcoCyc:G7323-MONOMER |
Protein family/group databases
MEROPSi | I39.008 |
Names & Taxonomyi
Protein namesi | Recommended name: Alpha-2-macroglobulin2 PublicationsAlternative name(s): ECAM1 Publication |
Gene namesi | Name:yfhM Ordered Locus Names:b2520, JW2504 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cell inner membrane 2 Publications; Lipid-anchor PROSITE-ProRule annotation; Periplasmic side 1 Publication
GO - Cellular componenti
- anchored component of external side of plasma membrane Source: EcoCyc
- extracellular space Source: InterPro
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 17 | PROSITE-ProRule annotationAdd BLAST | 17 | |
ChainiPRO_0000013775 | 18 – 1653 | Alpha-2-macroglobulinAdd BLAST | 1636 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 18 | N-palmitoyl cysteinePROSITE-ProRule annotation | 1 | |
Lipidationi | 18 | S-diacylglycerol cysteinePROSITE-ProRule annotation | 1 | |
Cross-linki | 1187 ↔ 1190 | Isoglutamyl cysteine thioester (Cys-Gln)1 Publication |
Keywords - PTMi
Lipoprotein, Palmitate, Thioester bondProteomic databases
jPOSTi | P76578 |
PaxDbi | P76578 |
PRIDEi | P76578 |
Interactioni
Subunit structurei
May form homooligomers.
1 PublicationProtein-protein interaction databases
BioGRIDi | 4260803, 213 interactors |
DIPi | DIP-28064N |
IntActi | P76578, 15 interactors |
STRINGi | 511145.b2520 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P76578 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 1559 – 1589 | Sequence analysisAdd BLAST | 31 |
Domaini
Cleavage causes major structural rearrangement of more than half the 13-domain structure from a native to a compact induced form.1 Publication
Sequence similaritiesi
Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family. Bacterial alpha-2-macroglobulin subfamily. [View classification]Curated
Keywords - Domaini
Coiled coil, SignalPhylogenomic databases
eggNOGi | COG2373, Bacteria |
HOGENOMi | CLU_000965_1_0_6 |
InParanoidi | P76578 |
PhylomeDBi | P76578 |
Family and domain databases
InterProi | View protein in InterPro IPR026284, A2-macglob_dom_prot_bac IPR011625, A2M_N_BRD IPR040639, A2MG_MG1 IPR011626, Alpha-macroglobulin_TED IPR021868, Alpha_2_Macroglob_MG3 IPR041203, Bact_A2M_MG5 IPR041462, Bact_A2M_MG6 IPR041246, Bact_MG10 IPR001599, Macroglobln_a2 IPR002890, MG2 IPR008930, Terpenoid_cyclase/PrenylTrfase |
Pfami | View protein in Pfam PF00207, A2M, 1 hit PF07703, A2M_BRD, 1 hit PF17970, bMG1, 1 hit PF17973, bMG10, 1 hit PF11974, bMG3, 1 hit PF17972, bMG5, 1 hit PF17962, bMG6, 1 hit PF01835, MG2, 1 hit PF07678, TED_complement, 1 hit |
PIRSFi | PIRSF038980, A2M_bac, 1 hit |
SMARTi | View protein in SMART SM01360, A2M, 1 hit SM01359, A2M_N_2, 1 hit |
SUPFAMi | SSF48239, SSF48239, 1 hit |
PROSITEi | View protein in PROSITE PS51257, PROKAR_LIPOPROTEIN, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P76578-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKKLRVAACM LMLALAGCDN NDNAPTAVKK DAPSEVTKAA SSENASSAKL
60 70 80 90 100
SVPERQKLAQ QSAGKVLTLL DLSEVQLDGA ATLVLTFSIP LDPDQDFSRV
110 120 130 140 150
IHVVDKKSGK VDGAWELSDN LKELRLRHLE PKRDLIVTIG KEVKALNNAT
160 170 180 190 200
FSKDYEKTIT TRDIQPSVGF ASRGSLLPGK VVEGLPVMAL NVNNVDVNFF
210 220 230 240 250
RVKPESLPAF ISQWEYRNSL ANWQSDKLLQ MADLVYTGRF DLNPARNTRE
260 270 280 290 300
KLLLPLGDIK PLQQAGVYLA VMNQAGRYDY SNPATLFTLS DIGVSAHRYH
310 320 330 340 350
NRLDIFTQSL ENGAAQQGIE VSLLNEKGQT LTQATSDAQG HVQLENDKNA
360 370 380 390 400
ALLLARKDGQ TTLLDLKLPA LDLAEFNIAG APGYSKQFFM FGPRDLYRPG
410 420 430 440 450
ETVILNGLLR DADGKALPNQ PIKLDVIKPD GQVLRSVVSQ PENGLYHFTW
460 470 480 490 500
PLDSNAATGM WHIRANTGDN QYRMWDFHVE DFMPERMALN LTGEKTPLTP
510 520 530 540 550
KDEVKFSVVG YYLYGAPANG NTLQGQLFLR PLREAVSALP GFEFGDIAAE
560 570 580 590 600
NLSRTLDEVQ LTLDDKGRGE VSTESQWKET HSPLQVIFQG SLLESGGRPV
610 620 630 640 650
TRRAEQAIWP ADALPGIRPQ FASKSVYDYR TDSTVKQPIV DEGSNAAFDI
660 670 680 690 700
VYSDAQGVKK AVSGLQVRLI RERRDYYWNW SEDEGWQSQF DQKDLIENEQ
710 720 730 740 750
TLDLKADETG KVSFPVEWGA YRLEVKAPNE AVSSVRFWAG YSWQDNSDGS
760 770 780 790 800
GAVRPDRVTL KLDKASYRPG DTIKLHIAAP TAGKGYAMVE SSEGPLWWQE
810 820 830 840 850
IDVRAQGLDL TIPVDKTWNR HDLYLSTLVV RPGDKSRSAT PKRAVGVLHL
860 870 880 890 900
PLGDENRRLD LALETPAKMR PNQPLTVKIK ASTKNGEKPK QVNVLVSAVD
910 920 930 940 950
SGVLNITDYV TPDPWQAFFG QKRYGADIYD IYGQVIEGQG RLAALRFGGD
960 970 980 990 1000
GDELKRGGKP PVNHVNIVVQ QALPVTLNEQ GEGSVTLPIG DFNGELRVMA
1010 1020 1030 1040 1050
QAWTADDFGS NESKVIVAAP VIAELNMPRF MASGDTSRLT LDITNLTDKP
1060 1070 1080 1090 1100
QKLNVALTAS GLLELVSDSP AAVELAPGVR TTLFIPVRAL PGYGDGEIQA
1110 1120 1130 1140 1150
TISGLALPGE TVADQHKQWK IGVRPAFPAQ TVNYGTALQP GETWAIPADG
1160 1170 1180 1190 1200
LQNFSPVTLE GQLLLSGKPP LNIARYIKEL KAYPYGCLEQ TASGLFPSLY
1210 1220 1230 1240 1250
TNAAQLQALG IKGDSDEKRR ASVDIGISRL LQMQRDNGGF ALWDKNGDEE
1260 1270 1280 1290 1300
YWLTAYVMDF LVRAGEQGYS VPTDAINRGN ERLLRYLQDP GMMSIPYADN
1310 1320 1330 1340 1350
LKASKFAVQS YAALVLARQQ KAPLGALREI WEHRADAASG LPLLQLGVAL
1360 1370 1380 1390 1400
KTMGDATRGE EAIALALKTP RNSDERIWLG DYGSSLRDNA LMLSLLEENK
1410 1420 1430 1440 1450
LLPDEQYTLL NTLSQQAFGE RWLSTQESNA LFLAARTIQD LPGKWQAQTS
1460 1470 1480 1490 1500
FSAEQLTGEK AQNSNLNSDQ LVTLQVSNSG DQPLWLRMDA SGYPQSAPLP
1510 1520 1530 1540 1550
ANNVLQIERH ILGTDGKSKS LDSLRSGDLV LVWLQVKASN SVPDALVVDL
1560 1570 1580 1590 1600
LPAGLELENQ NLANGSASLE QSGGEVQNLL NQMQQASIKH IEFRDDRFVA
1610 1620 1630 1640 1650
AVAVDEYQPV TLVYLARAVT PGTYQVPQPM VESMYVPQWR ATGAAEDLLI
VRP
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC75573.1 AP009048 Genomic DNA Translation: BAE76729.1 |
PIRi | G65028 |
RefSeqi | NP_417015.1, NC_000913.3 WP_000736312.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC75573; AAC75573; b2520 BAE76729; BAE76729; BAE76729 |
GeneIDi | 947302 |
KEGGi | ecj:JW2504 eco:b2520 |
PATRICi | fig|511145.12.peg.2619 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC75573.1 AP009048 Genomic DNA Translation: BAE76729.1 |
PIRi | G65028 |
RefSeqi | NP_417015.1, NC_000913.3 WP_000736312.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4RTD | X-ray | 3.65 | A | 23-1653 | [»] | |
4ZIQ | X-ray | 2.55 | A | 40-1653 | [»] | |
4ZIU | X-ray | 2.70 | A | 1018-1653 | [»] | |
4ZJG | X-ray | 2.30 | A | 40-385 | [»] | |
4ZJH | X-ray | 1.60 | A | 163-368 | [»] | |
5A42 | electron microscopy | 16.00 | A | 40-1653 | [»] | |
SMRi | P76578 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260803, 213 interactors |
DIPi | DIP-28064N |
IntActi | P76578, 15 interactors |
STRINGi | 511145.b2520 |
Protein family/group databases
MEROPSi | I39.008 |
Proteomic databases
jPOSTi | P76578 |
PaxDbi | P76578 |
PRIDEi | P76578 |
Genome annotation databases
EnsemblBacteriai | AAC75573; AAC75573; b2520 BAE76729; BAE76729; BAE76729 |
GeneIDi | 947302 |
KEGGi | ecj:JW2504 eco:b2520 |
PATRICi | fig|511145.12.peg.2619 |
Organism-specific databases
EchoBASEi | EB3175 |
Phylogenomic databases
eggNOGi | COG2373, Bacteria |
HOGENOMi | CLU_000965_1_0_6 |
InParanoidi | P76578 |
PhylomeDBi | P76578 |
Enzyme and pathway databases
BioCyci | EcoCyc:G7323-MONOMER |
Miscellaneous databases
PROi | PR:P76578 |
Family and domain databases
InterProi | View protein in InterPro IPR026284, A2-macglob_dom_prot_bac IPR011625, A2M_N_BRD IPR040639, A2MG_MG1 IPR011626, Alpha-macroglobulin_TED IPR021868, Alpha_2_Macroglob_MG3 IPR041203, Bact_A2M_MG5 IPR041462, Bact_A2M_MG6 IPR041246, Bact_MG10 IPR001599, Macroglobln_a2 IPR002890, MG2 IPR008930, Terpenoid_cyclase/PrenylTrfase |
Pfami | View protein in Pfam PF00207, A2M, 1 hit PF07703, A2M_BRD, 1 hit PF17970, bMG1, 1 hit PF17973, bMG10, 1 hit PF11974, bMG3, 1 hit PF17972, bMG5, 1 hit PF17962, bMG6, 1 hit PF01835, MG2, 1 hit PF07678, TED_complement, 1 hit |
PIRSFi | PIRSF038980, A2M_bac, 1 hit |
SMARTi | View protein in SMART SM01360, A2M, 1 hit SM01359, A2M_N_2, 1 hit |
SUPFAMi | SSF48239, SSF48239, 1 hit |
PROSITEi | View protein in PROSITE PS51257, PROKAR_LIPOPROTEIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | A2MG_ECOLI | |
Accessioni | P76578Primary (citable) accession number: P76578 Secondary accession number(s): Q2MAH7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 27, 2002 |
Last sequence update: | February 1, 1997 | |
Last modified: | December 2, 2020 | |
This is version 145 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Uncharacterized protein families (UPF)
List of uncharacterized protein family (UPF) entries