UniProtKB - P76562 (TMCA_ECOLI)
tRNA(Met) cytidine acetyltransferase TmcA
tmcA
Functioni
Catalyzes the formation of N4-acetylcytidine (ac4C) at the wobble position of tRNA(Met), by using acetyl-CoA as an acetyl donor and ATP (or GTP). It recognizes the wobble base of tRNA(Met), thus distinguishing between tRNA(Met) and the structurally similar tRNA(Ile2) (PubMed:18668122, PubMed:19322199).
Could use an RNA helicase motor driven by ATP hydrolysis to deliver the wobble base of tRNA(Met) to the acetyltransferase domain of TmcA (Probable).
1 Publication2 PublicationsAlso functions as a lysine 2-hydroxyisobutyryltransferase to regulate transcription. Can specifically catalyze the 2-hydroxyisobutyrylation (Khib) of the DNA-binding protein H-NS. Hydroxyisobutyrylation of H-NS decreases its DNA-binding activity, promotes the expression of acid-resistance genes and enhances bacterial survival under extreme acid stress.
1 PublicationMiscellaneous
Catalytic activityi
- acetyl-CoA + ATP + cytidine34 in elongator tRNAMet + H2O = ADP + CoA + H+ + N4-acetylcytidine34 in elongator tRNAMet + phosphateUniRule annotation2 PublicationsEC:2.3.1.193UniRule annotation2 Publications
- 2-hydroxyisobutanoyl-CoA + L-lysyl-[protein] = CoA + H+ + N6-(2-hydroxyisobutanoyl)-L-lysyl-[protein]1 PublicationThis reaction proceeds in the forward1 Publication direction.
Activity regulationi
Kineticsi
- KM=2.93 µM for ATP (in the absence of both acetyl-CoA and tRNA(Met))1 Publication
- KM=4.16 µM for ATP (in the presence of both acetyl-CoA and tRNA(Met))1 Publication
- KM=117 µM for GTP (in the absence of both acetyl-CoA and tRNA(Met))1 Publication
- KM=66.6 µM for GTP (in the presence of both acetyl-CoA and tRNA(Met))1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 180 | ATPUniRule annotation1 Publication | 1 | |
Binding sitei | 319 | ATPUniRule annotation1 Publication | 1 | |
Binding sitei | 499 | Acetyl-CoAUniRule annotation1 Publication | 1 | |
Sitei | 502 | Important for lysine 2-hydroxyisobutyryltransferase activity1 Publication | 1 | |
Binding sitei | 506 | Acetyl-CoAUniRule annotation1 Publication | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 202 – 211 | ATPUniRule annotation1 Publication | 10 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- tRNA binding Source: EcoCyc
- tRNA N-acetyltransferase activity Source: EcoCyc
GO - Biological processi
- tRNA acetylation Source: EcoCyc
- tRNA wobble cytosine modification Source: EcoCyc
Keywordsi
Molecular function | Acyltransferase, RNA-binding, Transferase, tRNA-binding |
Biological process | tRNA processing |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:G7297-MONOMER |
BRENDAi | 2.3.1.193, 2026 |
SABIO-RKi | P76562 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:tmcAUniRule annotation Synonyms:ypfI Ordered Locus Names:b2474, JW2459 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 287 | T → A: Loss of ATPase activity, but no change in tRNA-binding. 1 Publication | 1 | |
Mutagenesisi | 291 | Y → A: Loss of ATPase activity, but no change in tRNA-binding. 1 Publication | 1 | |
Mutagenesisi | 292 | E → A: Loss of ATPase activity, but no change in tRNA-binding. 1 Publication | 1 | |
Mutagenesisi | 296 | R → A: Decrease in ATPase activity and loss of tRNA-binding. 1 Publication | 1 | |
Mutagenesisi | 301 | K → A: Decrease in ATPase activity and loss of tRNA-binding. 1 Publication | 1 | |
Mutagenesisi | 319 | R → A: Loss of ATPase activity, but no change in tRNA-binding. Lack of hydroxyisobutyrylation activity. 2 Publications | 1 | |
Mutagenesisi | 327 | E → A: Loss of ATPase activity, but no change in tRNA-binding. 1 Publication | 1 | |
Mutagenesisi | 377 | H → A: Decrease in ATPase activity and loss of tRNA-binding. 1 Publication | 1 | |
Mutagenesisi | 387 | R → A: Abolishes the tRNA-binding capacity, but no loss of activity. 1 Publication | 1 | |
Mutagenesisi | 460 | R → A: Loss of tRNA-binding. 1 Publication | 1 | |
Mutagenesisi | 463 | V → A: Does not affect hydroxyisobutyrylation activity. 1 Publication | 1 | |
Mutagenesisi | 469 | R → A: Does not affect hydroxyisobutyrylation activity. 1 Publication | 1 | |
Mutagenesisi | 471 | G → A: Does not affect hydroxyisobutyrylation activity. 1 Publication | 1 | |
Mutagenesisi | 502 | R → A: Lack of hydroxyisobutyrylation activity. Has little effect on RNA acetylation activity. Alters binding with hydroxyisobutanoyl-CoA, but not with acetyl-CoA. 1 Publication | 1 | |
Mutagenesisi | 504 | W → A: Does not affect hydroxyisobutyrylation activity. No change in acetyl-CoA or hydroxyisobutanoyl-CoA binding. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000169239 | 1 – 671 | tRNA(Met) cytidine acetyltransferase TmcAAdd BLAST | 671 |
Proteomic databases
PaxDbi | P76562 |
PRIDEi | P76562 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4259195, 20 interactors |
DIPi | DIP-12825N |
IntActi | P76562, 8 interactors |
STRINGi | 511145.b2474 |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P76562 |
SMRi | P76562 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P76562 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 356 – 531 | N-acetyltransferaseUniRule annotationAdd BLAST | 176 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 461 – 463 | Acetyl-CoA bindingUniRule annotation1 Publication | 3 | |
Regioni | 468 – 474 | Acetyl-CoA bindingUniRule annotation1 Publication | 7 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG1444, Bacteria |
HOGENOMi | CLU_004652_1_1_6 |
InParanoidi | P76562 |
OMAi | NEPDDLG |
PhylomeDBi | P76562 |
Family and domain databases
Gene3Di | 1.20.120.890, 1 hit 3.40.50.300, 1 hit |
HAMAPi | MF_01886, tRNA_acetyltr_TmcA, 1 hit |
InterProi | View protein in InterPro IPR016181, Acyl_CoA_acyltransferase IPR000182, GNAT_dom IPR007807, Helicase_dom IPR027417, P-loop_NTPase IPR032672, TmcA/NAT10/Kre33 IPR038321, TmcA_C_sf IPR013562, TmcA_N IPR033442, TmcA_tRNA_bind IPR024914, tRNA_acetyltr_TmcA |
PANTHERi | PTHR10925, PTHR10925, 2 hits |
Pfami | View protein in Pfam PF08351, DUF1726, 1 hit PF13718, GNAT_acetyltr_2, 1 hit PF05127, Helicase_RecD, 1 hit PF17176, tRNA_bind_3, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF55729, SSF55729, 1 hit |
PROSITEi | View protein in PROSITE PS51186, GNAT, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MAELTALHTL TAQMKREGIR RLLVLSGEEG WCFEHTLKLR DALPGDWLWI
60 70 80 90 100
SPRPDAENHC SPSALQTLLG REFRHAVFDA RHGFDAAAFA ALSGTLKAGS
110 120 130 140 150
WLVLLLPVWE EWENQPDADS LRWSDCPDPI ATPHFVQHLK RVLTADNEAI
160 170 180 190 200
LWRQNQPFSL AHFTPRTDWY PATGAPQPEQ QQLLKQLMTM PPGVAAVTAA
210 220 230 240 250
RGRGKSALAG QLISRIAGRA IVTAPAKAST DVLAQFAGEK FRFIAPDALL
260 270 280 290 300
ASDEQADWLV VDEAAAIPAP LLHQLVSRFP RTLLTTTVQG YEGTGRGFLL
310 320 330 340 350
KFCARFPHLH RFELQQPIRW AQGCPLEKMV SEALVFDDEN FTHTPQGNIV
360 370 380 390 400
ISAFEQTLWQ SDPETPLKVY QLLSGAHYRT SPLDLRRMMD APGQHFLQAA
410 420 430 440 450
GENEIAGALW LVDEGGLSQQ LSQAVWAGFR RPRGNLVAQS LAAHGNNPLA
460 470 480 490 500
ATLRGRRVSR IAVHPARQRE GTGRQLIAGA LQYTQDLDYL SVSFGYTGEL
510 520 530 540 550
WRFWQRCGFV LVRMGNHREA SSGCYTAMAL LPMSDAGKQL AEREHYRLRR
560 570 580 590 600
DAQALAQWNG ETLPVDPLND AVLSDDDWLE LAGFAFAHRP LLTSLGCLLR
610 620 630 640 650
LLQTSELALP ALRGRLQKNA SDAQLCTTLK LSGRKMLLVR QREEAAQALF
660 670
ALNDVRTERL RDRITQWQLF H
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC75527.1 AP009048 Genomic DNA Translation: BAA16352.2 |
PIRi | A65023 |
RefSeqi | NP_416969.1, NC_000913.3 WP_000829360.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC75527; AAC75527; b2474 BAA16352; BAA16352; BAA16352 |
GeneIDi | 946053 |
KEGGi | ecj:JW2459 eco:b2474 |
PATRICi | fig|1411691.4.peg.4265 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC75527.1 AP009048 Genomic DNA Translation: BAA16352.2 |
PIRi | A65023 |
RefSeqi | NP_416969.1, NC_000913.3 WP_000829360.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2ZPA | X-ray | 2.35 | A/B | 1-671 | [»] | |
AlphaFoldDBi | P76562 | |||||
SMRi | P76562 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259195, 20 interactors |
DIPi | DIP-12825N |
IntActi | P76562, 8 interactors |
STRINGi | 511145.b2474 |
Proteomic databases
PaxDbi | P76562 |
PRIDEi | P76562 |
Genome annotation databases
EnsemblBacteriai | AAC75527; AAC75527; b2474 BAA16352; BAA16352; BAA16352 |
GeneIDi | 946053 |
KEGGi | ecj:JW2459 eco:b2474 |
PATRICi | fig|1411691.4.peg.4265 |
Organism-specific databases
EchoBASEi | EB3948 |
Phylogenomic databases
eggNOGi | COG1444, Bacteria |
HOGENOMi | CLU_004652_1_1_6 |
InParanoidi | P76562 |
OMAi | NEPDDLG |
PhylomeDBi | P76562 |
Enzyme and pathway databases
BioCyci | EcoCyc:G7297-MONOMER |
BRENDAi | 2.3.1.193, 2026 |
SABIO-RKi | P76562 |
Miscellaneous databases
EvolutionaryTracei | P76562 |
PROi | PR:P76562 |
Family and domain databases
Gene3Di | 1.20.120.890, 1 hit 3.40.50.300, 1 hit |
HAMAPi | MF_01886, tRNA_acetyltr_TmcA, 1 hit |
InterProi | View protein in InterPro IPR016181, Acyl_CoA_acyltransferase IPR000182, GNAT_dom IPR007807, Helicase_dom IPR027417, P-loop_NTPase IPR032672, TmcA/NAT10/Kre33 IPR038321, TmcA_C_sf IPR013562, TmcA_N IPR033442, TmcA_tRNA_bind IPR024914, tRNA_acetyltr_TmcA |
PANTHERi | PTHR10925, PTHR10925, 2 hits |
Pfami | View protein in Pfam PF08351, DUF1726, 1 hit PF13718, GNAT_acetyltr_2, 1 hit PF05127, Helicase_RecD, 1 hit PF17176, tRNA_bind_3, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF55729, SSF55729, 1 hit |
PROSITEi | View protein in PROSITE PS51186, GNAT, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TMCA_ECOLI | |
Accessioni | P76562Primary (citable) accession number: P76562 Secondary accession number(s): P76972 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | February 1, 1997 | |
Last modified: | May 25, 2022 | |
This is version 148 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families