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Protein

NADP-dependent malic enzyme

Gene

maeB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Cannot use NAD+.

Catalytic activityi

(S)-malate + NADP+ = pyruvate + CO2 + NADPH.
Oxaloacetate = pyruvate + CO2.

Cofactori

Mg2+1 Publication, Mn2+1 PublicationNote: Divalent metal cations. Prefers magnesium or manganese.1 Publication

Enzyme regulationi

Inhibited by 4 mM Mg2+ and acetyl-CoA, competitively inhibited by fumarate and oxaloacetate. Activated by glutamate and aspartate, glucose-6-phosphate, acetyl-phosphate and 2 mM KCl.1 Publication

Kineticsi

  1. KM=3.41 mM for L-malate1 Publication
  2. KM=0.0415 mM for NADP1 Publication
  3. KM=6.21 mM for pyruvate1 Publication

    pH dependencei

    Optimum pH is 7.5 for L-malate.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei94Proton acceptorBy similarity1
    Metal bindingi136Divalent metal cationBy similarity1
    Metal bindingi137Divalent metal cationBy similarity1
    Binding sitei162NADBy similarity1
    Binding sitei288NADBy similarity1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi76 – 83NADPBy similarity8

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionMultifunctional enzyme, Oxidoreductase
    LigandMetal-binding, NADP

    Enzyme and pathway databases

    BioCyciEcoCyc:MALIC-NADP-MONOMER
    MetaCyc:MALIC-NADP-MONOMER
    BRENDAi1.1.1.40 2026

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADP-dependent malic enzyme (EC:1.1.1.40)
    Short name:
    NADP-ME
    Gene namesi
    Name:maeB
    Synonyms:ypfF
    Ordered Locus Names:b2463, JW2447
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG14193 maeB

    Subcellular locationi

    GO - Cellular componenti

    • cytosol Source: UniProtKB

    Pathology & Biotechi

    Chemistry databases

    ChEMBLiCHEMBL1687685

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001602421 – 759NADP-dependent malic enzymeAdd BLAST759

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei56N6-acetyllysine1 Publication1

    Keywords - PTMi

    Acetylation

    Proteomic databases

    EPDiP76558
    PaxDbiP76558
    PRIDEiP76558

    PTM databases

    iPTMnetiP76558

    Interactioni

    Subunit structurei

    Homooligomer, possibly an octamer.

    Protein-protein interaction databases

    BioGridi4260925, 8 interactors
    DIPiDIP-10141N
    IntActiP76558, 10 interactors
    STRINGi316385.ECDH10B_2628

    Chemistry databases

    BindingDBiP76558

    Structurei

    3D structure databases

    ProteinModelPortaliP76558
    SMRiP76558
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni1 – 428Malic enzymeAdd BLAST428
    Regioni429 – 759Phosphate acetyltransferase; required for oligomerization, inhibition by acetyl-CoA and activation by glutamate, aspartate, and glucose-6-phosphateAdd BLAST331

    Domaini

    The-C-terminal phosphate acetyltransferase domain is responsible for oligomerization, and is responsible for inhibition by acetyl-CoA and activation by glutamate, aspartate, and glucose-6-phosphate as shown by its deletion. The isolated domain does not catalyze the interconversion of acetyl-CoA and acetyl-phosphate.1 Publication

    Sequence similaritiesi

    In the N-terminal section; belongs to the malic enzymes family.Curated
    In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family.Curated

    Phylogenomic databases

    eggNOGiENOG4105C6K Bacteria
    COG0280 LUCA
    COG0281 LUCA
    HOGENOMiHOG000132448
    InParanoidiP76558
    KOiK00029
    OMAiKFPFSKL
    PhylomeDBiP76558

    Family and domain databases

    Gene3Di3.40.50.10380, 1 hit
    InterProiView protein in InterPro
    IPR015884 Malic_enzyme_CS
    IPR012301 Malic_N_dom
    IPR037062 Malic_N_dom_sf
    IPR012302 Malic_NAD-bd
    IPR012188 ME_PTA
    IPR036291 NAD(P)-bd_dom_sf
    IPR002505 PTA_PTB
    PfamiView protein in Pfam
    PF00390 malic, 1 hit
    PF03949 Malic_M, 1 hit
    PF01515 PTA_PTB, 1 hit
    PIRSFiPIRSF036684 ME_PTA, 1 hit
    SMARTiView protein in SMART
    SM01274 malic, 1 hit
    SM00919 Malic_M, 1 hit
    SUPFAMiSSF51735 SSF51735, 1 hit
    PROSITEiView protein in PROSITE
    PS00331 MALIC_ENZYMES, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P76558-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MDDQLKQSAL DFHEFPVPGK IQVSPTKPLA TQRDLALAYS PGVAAPCLEI
    60 70 80 90 100
    EKDPLKAYKY TARGNLVAVI SNGTAVLGLG NIGALAGKPV MEGKGVLFKK
    110 120 130 140 150
    FAGIDVFDIE VDELDPDKFI EVVAALEPTF GGINLEDIKA PECFYIEQKL
    160 170 180 190 200
    RERMNIPVFH DDQHGTAIIS TAAILNGLRV VEKNISDVRM VVSGAGAAAI
    210 220 230 240 250
    ACMNLLVALG LQKHNIVVCD SKGVIYQGRE PNMAETKAAY AVVDDGKRTL
    260 270 280 290 300
    DDVIEGADIF LGCSGPKVLT QEMVKKMARA PMILALANPE PEILPPLAKE
    310 320 330 340 350
    VRPDAIICTG RSDYPNQVNN VLCFPFIFRG ALDVGATAIN EEMKLAAVRA
    360 370 380 390 400
    IAELAHAEQS EVVASAYGDQ DLSFGPEYII PKPFDPRLIV KIAPAVAKAA
    410 420 430 440 450
    MESGVATRPI ADFDVYIDKL TEFVYKTNLF MKPIFSQARK APKRVVLPEG
    460 470 480 490 500
    EEARVLHATQ ELVTLGLAKP ILIGRPNVIE MRIQKLGLQI KAGVDFEIVN
    510 520 530 540 550
    NESDPRFKEY WTEYFQIMKR RGVTQEQAQR ALISNPTVIG AIMVQRGEAD
    560 570 580 590 600
    AMICGTVGDY HEHFSVVKNV FGYRDGVHTA GAMNALLLPS GNTFIADTYV
    610 620 630 640 650
    NDEPDAEELA EITLMAAETV RRFGIEPRVA LLSHSNFGSS DCPSSSKMRQ
    660 670 680 690 700
    ALELVRERAP ELMIDGEMHG DAALVEAIRN DRMPDSSLKG SANILVMPNM
    710 720 730 740 750
    EAARISYNLL RVSSSEGVTV GPVLMGVAKP VHVLTPIASV RRIVNMVALA

    VVEAQTQPL
    Length:759
    Mass (Da):82,417
    Last modified:February 1, 1997 - v1
    Checksum:i9A8B67D9635E25BA
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U00096 Genomic DNA Translation: AAC75516.1
    AP009048 Genomic DNA Translation: BAA16337.1
    PIRiF65021
    RefSeqiNP_416958.1, NC_000913.3
    WP_000342644.1, NZ_LN832404.1

    Genome annotation databases

    EnsemblBacteriaiAAC75516; AAC75516; b2463
    BAA16337; BAA16337; BAA16337
    GeneIDi946947
    KEGGiecj:JW2447
    eco:b2463
    PATRICifig|1411691.4.peg.4277

    Similar proteinsi

    Entry informationi

    Entry nameiMAO2_ECOLI
    AccessioniPrimary (citable) accession number: P76558
    Secondary accession number(s): P78200, P78201
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: February 1, 1997
    Last modified: March 28, 2018
    This is version 161 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

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