UniProtKB - P76535 (MURQ_ECOLI)
Protein
N-acetylmuramic acid 6-phosphate etherase
Gene
murQ
Organism
Escherichia coli (strain K12)
Status
Functioni
Specifically catalyzes the cleavage of the D-lactyl ether substituent of MurNAc 6-phosphate, producing GlcNAc 6-phosphate and D-lactate. Is required for growth on MurNAc as the sole source of carbon and energy. Together with AnmK, is also required for the utilization of anhydro-N-acetylmuramic acid (anhMurNAc) either imported from the medium or derived from its own cell wall murein, and thus plays a role in cell wall recycling.2 Publications
Miscellaneous
A lyase-type mechanism (elimination/hydration) is suggested for the cleavage of the lactyl ether bond of MurNAc 6-phosphate, with the formation of an alpha,beta-unsaturated aldehyde intermediate with (E)-stereochemistry, followed by the syn addition of water to give product.
Catalytic activityi
- H2O + N-acetyl-D-muramate 6-phosphate = (R)-lactate + N-acetyl-D-glucosamine 6-phosphate1 PublicationEC:4.2.1.1261 Publication
Kineticsi
kcat is 5.7 s(-1).
- KM=1.20 mM for N-acetylmuramic acid 6-phosphate1 Publication
: 1,6-anhydro-N-acetylmuramate degradation Pathwayi
This protein is involved in the pathway 1,6-anhydro-N-acetylmuramate degradation, which is part of Amino-sugar metabolism.View all proteins of this organism that are known to be involved in the pathway 1,6-anhydro-N-acetylmuramate degradation and in Amino-sugar metabolism.
Pathwayi: N-acetylmuramate degradation
This protein is involved in the pathway N-acetylmuramate degradation, which is part of Amino-sugar metabolism.View all proteins of this organism that are known to be involved in the pathway N-acetylmuramate degradation and in Amino-sugar metabolism.
Pathwayi: peptidoglycan recycling
This protein is involved in the pathway peptidoglycan recycling, which is part of Cell wall biogenesis.View all proteins of this organism that are known to be involved in the pathway peptidoglycan recycling and in Cell wall biogenesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 83 | Proton donor1 Publication | 1 | |
Active sitei | 114 | 1 Publication | 1 |
GO - Molecular functioni
- carbohydrate derivative binding Source: InterPro
- carbon-oxygen lyase activity Source: EcoCyc
- ether hydrolase activity Source: EcoliWiki
GO - Biological processi
- 1,6-anhydro-N-acetyl-beta-muramic acid catabolic process Source: UniProtKB-UniPathway
- amino sugar catabolic process Source: EcoCyc
- carbohydrate metabolic process Source: UniProtKB-KW
- N-acetylmuramic acid catabolic process Source: UniProtKB-UniPathway
- peptidoglycan turnover Source: EcoCyc
Keywordsi
Molecular function | Lyase |
Biological process | Carbohydrate metabolism |
Enzyme and pathway databases
BioCyci | EcoCyc:G7263-MONOMER MetaCyc:G7263-MONOMER |
UniPathwayi | UPA00342 UPA00343 UPA00544 |
Names & Taxonomyi
Protein namesi | Recommended name: N-acetylmuramic acid 6-phosphate etherase (EC:4.2.1.126)Short name: MurNAc-6-P etherase Alternative name(s): N-acetylmuramic acid 6-phosphate hydrolase N-acetylmuramic acid 6-phosphate lyase |
Gene namesi | Name:murQ Synonyms:yfeU Ordered Locus Names:b2428, JW2421 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
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Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 83 | E → A: 10000-fold reduction in catalytic activity. 1 Publication | 1 | |
Mutagenesisi | 83 | E → Q: Loss of catalytic activity. 1 Publication | 1 | |
Mutagenesisi | 114 | E → Q: 2000-fold reduction in catalytic activity and 4-fold increase in substrate affinity. 1 Publication | 1 | |
Mutagenesisi | 115 | D → N: 7-fold reduction in catalytic activity and 1.5-fold increase in substrate affinity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000214829 | 1 – 298 | N-acetylmuramic acid 6-phosphate etheraseAdd BLAST | 298 |
Proteomic databases
jPOSTi | P76535 |
PaxDbi | P76535 |
PRIDEi | P76535 |
Expressioni
Inductioni
Induced by MurNAc 6-phosphate that releases the repressor MurR from the DNA. Also up-regulated by the cAMP receptor protein crp via the binding of crp-cAMP to a class I site upstream of the murQ promoter. Repressed by MurR in the absence of MurNAc 6-phosphate.1 Publication
Interactioni
Subunit structurei
Homodimer.
1 PublicationProtein-protein interaction databases
BioGRIDi | 4260569, 7 interactors |
IntActi | P76535, 6 interactors |
STRINGi | 511145.b2428 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 55 – 218 | SISAdd BLAST | 164 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG2103, Bacteria |
HOGENOMi | CLU_049049_1_1_6 |
InParanoidi | P76535 |
PhylomeDBi | P76535 |
Family and domain databases
CDDi | cd05007, SIS_Etherase, 1 hit |
HAMAPi | MF_00068, MurQ, 1 hit |
InterProi | View protein in InterPro IPR005488, Etherase_MurQ IPR005486, Glucokinase_regulatory_CS IPR040190, MURQ/GCKR IPR001347, SIS |
PANTHERi | PTHR10088, PTHR10088, 1 hit PTHR10088:SF5, PTHR10088:SF5, 1 hit |
Pfami | View protein in Pfam PF01380, SIS, 1 hit |
TIGRFAMsi | TIGR00274, TIGR00274, 1 hit |
PROSITEi | View protein in PROSITE PS01272, GCKR, 1 hit PS51464, SIS, 1 hit |
i Sequence
Sequence statusi: Complete.
P76535-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQFEKMITEG SNTASAEIDR VSTLEMCRII NDEDKTVPLA VERVLPDIAA
60 70 80 90 100
AIDVIHAQVS GGGRLIYLGA GTSGRLGILD ASECPPTYGV KPGLVVGLIA
110 120 130 140 150
GGEYAIQHAV EGAEDSREGG VNDLKNINLT AQDVVVGIAA SGRTPYVIAG
160 170 180 190 200
LEYARQLGCR TVGISCNPGS AVSTTAEFAI TPIVGAEVVT GSSRMKAGTA
210 220 230 240 250
QKLVLNMLST GLMIKSGKVF GNLMVDVVAT NEKLHVRQVN IVKNATGCSA
260 270 280 290
EQAEAALIAC ERNCKTAIVM VLKNLDAAEA KKRLDQHGGF IRQVLDKE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC75481.1 AP009048 Genomic DNA Translation: BAA16312.2 |
PIRi | C65017 |
RefSeqi | NP_416923.1, NC_000913.3 WP_001159160.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC75481; AAC75481; b2428 BAA16312; BAA16312; BAA16312 |
GeneIDi | 946893 |
KEGGi | ecj:JW2421 eco:b2428 |
PATRICi | fig|1411691.4.peg.4303 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC75481.1 AP009048 Genomic DNA Translation: BAA16312.2 |
PIRi | C65017 |
RefSeqi | NP_416923.1, NC_000913.3 WP_001159160.1, NZ_LN832404.1 |
3D structure databases
SMRi | P76535 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4260569, 7 interactors |
IntActi | P76535, 6 interactors |
STRINGi | 511145.b2428 |
Proteomic databases
jPOSTi | P76535 |
PaxDbi | P76535 |
PRIDEi | P76535 |
Genome annotation databases
EnsemblBacteriai | AAC75481; AAC75481; b2428 BAA16312; BAA16312; BAA16312 |
GeneIDi | 946893 |
KEGGi | ecj:JW2421 eco:b2428 |
PATRICi | fig|1411691.4.peg.4303 |
Organism-specific databases
EchoBASEi | EB3914 |
Phylogenomic databases
eggNOGi | COG2103, Bacteria |
HOGENOMi | CLU_049049_1_1_6 |
InParanoidi | P76535 |
PhylomeDBi | P76535 |
Enzyme and pathway databases
UniPathwayi | UPA00342 UPA00343 UPA00544 |
BioCyci | EcoCyc:G7263-MONOMER MetaCyc:G7263-MONOMER |
Miscellaneous databases
PROi | PR:P76535 |
Family and domain databases
CDDi | cd05007, SIS_Etherase, 1 hit |
HAMAPi | MF_00068, MurQ, 1 hit |
InterProi | View protein in InterPro IPR005488, Etherase_MurQ IPR005486, Glucokinase_regulatory_CS IPR040190, MURQ/GCKR IPR001347, SIS |
PANTHERi | PTHR10088, PTHR10088, 1 hit PTHR10088:SF5, PTHR10088:SF5, 1 hit |
Pfami | View protein in Pfam PF01380, SIS, 1 hit |
TIGRFAMsi | TIGR00274, TIGR00274, 1 hit |
PROSITEi | View protein in PROSITE PS01272, GCKR, 1 hit PS51464, SIS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MURQ_ECOLI | |
Accessioni | P76535Primary (citable) accession number: P76535 Secondary accession number(s): P76965, P76966, P76967 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | February 1, 1997 | |
Last modified: | December 2, 2020 | |
This is version 149 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families