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UniProtKB - P76518 (ACOCT_ECOLI)
Protein
Acetyl-CoA:oxalate CoA-transferase
Gene
yfdE
Organism
Escherichia coli (strain K12)
Status
Functioni
Involved in the catabolism of oxalate and in the adapatation to low pH. ACOCT serves to prime the oxalate-induced acid tolerance response (ATR) cycle by producing substrate for oxalyl-CoA decarboxylase (OXC) and formyl-coenzyme A transferase (FCOCT). Catalyzes the reversible conversion of acetyl-CoA and oxalate to oxalyl-CoA and acetate. It can also use formyl-CoA and oxalate to produce oxalyl-CoA and formate with significantly reduced specific activity.
1 PublicationCatalytic activityi
- EC:2.8.3.191 Publication
Kineticsi
kcat is 11 sec(-1) for the CoA-transferase activity with acetyl-CoA as substrate (at pH 6.7 and 25 degrees Celsius). kcat is 15 sec(-1) for the CoA-transferase activity with oxalate as substrate (at pH 6.7 and 25 degrees Celsius).
- KM=17 µM for acetyl-CoA (at pH 6.7 and 25 degrees Celsius)1 Publication
- KM=22 mM for oxalate (at pH 6.7 and 25 degrees Celsius)1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 233 | By similarity | 1 |
GO - Molecular functioni
- acetyl-CoA:oxalate CoA-transferase Source: EcoCyc
Keywordsi
Molecular function | Transferase |
Enzyme and pathway databases
BioCyci | EcoCyc:G7234-MONOMER |
BRENDAi | 2.8.3.19, 2026 |
Names & Taxonomyi
Protein namesi | Recommended name: Acetyl-CoA:oxalate CoA-transferase (EC:2.8.3.19)Short name: ACOCT Alternative name(s): Acetyl-coenzyme A transferase CoA:oxalate CoA-transferase |
Gene namesi | Name:yfdE Ordered Locus Names:b2371, JW2368 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000194725 | 1 – 381 | Acetyl-CoA:oxalate CoA-transferaseAdd BLAST | 381 |
Proteomic databases
PaxDbi | P76518 |
PRIDEi | P76518 |
Expressioni
Inductioni
By the acid response regulator EvgA.2 Publications
Interactioni
Subunit structurei
Homodimer.
1 PublicationProtein-protein interaction databases
BioGRIDi | 4259190, 3 interactors |
DIPi | DIP-11994N |
IntActi | P76518, 7 interactors |
STRINGi | 511145.b2371 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P76518 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the CoA-transferase III family.Curated
Phylogenomic databases
eggNOGi | COG1804, Bacteria |
HOGENOMi | CLU_033975_0_0_6 |
InParanoidi | P76518 |
PhylomeDBi | P76518 |
Family and domain databases
Gene3Di | 3.30.1540.10, 1 hit 3.40.50.10540, 1 hit |
InterProi | View protein in InterPro IPR003673, CoA-Trfase_fam_III IPR044855, CoA-Trfase_III_dom3_sf IPR023606, CoA-Trfase_III_dom_1_sf |
Pfami | View protein in Pfam PF02515, CoA_transf_3, 1 hit |
SUPFAMi | SSF89796, SSF89796, 1 hit |
i Sequence
Sequence statusi: Complete.
P76518-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTNNESKGPF EGLLVIDMTH VLNGPFGTQL LCNMGARVIK VEPPGHGDDT
60 70 80 90 100
RTFGPYVDGQ SLYYSFINHG KESVVLDLKN DHDKSIFINM LKQADVLAEN
110 120 130 140 150
FRPGTMEKLG FSWETLQEIN PRLIYASSSG FGHTGPLKDA PAYDTIIQAM
160 170 180 190 200
SGIMMETGYP DAPPVRVGTS LADLCGGVYL FSGIVSALYG REKSQRGAHV
210 220 230 240 250
DIAMFDATLS FLEHGLMAYI ATGKSPQRLG NRHPYMAPFD VFNTQDKPIT
260 270 280 290 300
ICCGNDKLFS ALCQALELTE LVNDPRFSSN ILRVQNQAIL KQYIERTLKT
310 320 330 340 350
QAAEVWLARI HEVGVPVAPL LSVAEAIKLP QTQARNMLIE AGGIMMPGNP
360 370 380
IKISGCADPH VMPGAATLDQ HGEQIRQEFS S
Sequence cautioni
The sequence D14008 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 294 | I → T in D14008 (PubMed:8125343).Curated | 1 | |
Sequence conflicti | 333 | Q → E in D14008 (PubMed:8125343).Curated | 1 | |
Sequence conflicti | 357 | A → T in D14008 (PubMed:8125343).Curated | 1 | |
Sequence conflicti | 368 | L → S in D14008 (PubMed:8125343).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC75430.2 AP009048 Genomic DNA Translation: BAA16242.1 D14008 Genomic DNA No translation available. |
PIRi | H65010 |
RefSeqi | NP_416872.4, NC_000913.3 WP_001296867.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC75430; AAC75430; b2371 BAA16242; BAA16242; BAA16242 |
GeneIDi | 66673759 946432 |
KEGGi | ecj:JW2368 eco:b2371 |
PATRICi | fig|1411691.4.peg.4358 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC75430.2 AP009048 Genomic DNA Translation: BAA16242.1 D14008 Genomic DNA No translation available. |
PIRi | H65010 |
RefSeqi | NP_416872.4, NC_000913.3 WP_001296867.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4HL6 | X-ray | 2.12 | A/B/C/D/E/F | 1-381 | [»] | |
SMRi | P76518 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259190, 3 interactors |
DIPi | DIP-11994N |
IntActi | P76518, 7 interactors |
STRINGi | 511145.b2371 |
Proteomic databases
PaxDbi | P76518 |
PRIDEi | P76518 |
Genome annotation databases
EnsemblBacteriai | AAC75430; AAC75430; b2371 BAA16242; BAA16242; BAA16242 |
GeneIDi | 66673759 946432 |
KEGGi | ecj:JW2368 eco:b2371 |
PATRICi | fig|1411691.4.peg.4358 |
Organism-specific databases
EchoBASEi | EB3069 |
Phylogenomic databases
eggNOGi | COG1804, Bacteria |
HOGENOMi | CLU_033975_0_0_6 |
InParanoidi | P76518 |
PhylomeDBi | P76518 |
Enzyme and pathway databases
BioCyci | EcoCyc:G7234-MONOMER |
BRENDAi | 2.8.3.19, 2026 |
Miscellaneous databases
PROi | PR:P76518 |
Family and domain databases
Gene3Di | 3.30.1540.10, 1 hit 3.40.50.10540, 1 hit |
InterProi | View protein in InterPro IPR003673, CoA-Trfase_fam_III IPR044855, CoA-Trfase_III_dom3_sf IPR023606, CoA-Trfase_III_dom_1_sf |
Pfami | View protein in Pfam PF02515, CoA_transf_3, 1 hit |
SUPFAMi | SSF89796, SSF89796, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ACOCT_ECOLI | |
Accessioni | P76518Primary (citable) accession number: P76518 Secondary accession number(s): P76946 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | August 29, 2001 | |
Last modified: | February 23, 2022 | |
This is version 155 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families