UniProtKB - P76503 (FADI_ECOLI)
Protein
3-ketoacyl-CoA thiolase FadI
Gene
fadI
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the final step of fatty acid oxidation in which acetyl-CoA is released and the CoA ester of a fatty acid two carbons shorter is formed. Strongly involved in the anaerobic degradation of long and medium-chain fatty acids in the presence of nitrate and weakly involved in the aerobic degradation of long-chain fatty acids.2 Publications
Catalytic activityi
- EC:2.3.1.16
: fatty acid beta-oxidation Pathwayi
This protein is involved in the pathway fatty acid beta-oxidation, which is part of Lipid metabolism.View all proteins of this organism that are known to be involved in the pathway fatty acid beta-oxidation and in Lipid metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 99 | Acyl-thioester intermediateBy similarity | 1 | |
Active sitei | 392 | Proton acceptorBy similarity | 1 | |
Active sitei | 422 | Proton acceptorBy similarity | 1 |
GO - Molecular functioni
- 3-hydroxyacyl-CoA dehydrogenase activity Source: EcoliWiki
- acetyl-CoA C-acetyltransferase activity Source: GO_Central
- acetyl-CoA C-acyltransferase activity Source: EcoliWiki
- enoyl-CoA hydratase activity Source: EcoliWiki
GO - Biological processi
- fatty acid beta-oxidation Source: EcoCyc
- fatty acid beta-oxidation, unsaturated, even number Source: EcoliWiki
Keywordsi
Molecular function | Acyltransferase, Transferase |
Biological process | Fatty acid metabolism, Lipid degradation, Lipid metabolism |
Enzyme and pathway databases
BioCyci | EcoCyc:G7213-MONOMER MetaCyc:G7213-MONOMER |
UniPathwayi | UPA00659 |
Names & Taxonomyi
Protein namesi | Recommended name: 3-ketoacyl-CoA thiolase FadI (EC:2.3.1.16)Alternative name(s): ACSs Acetyl-CoA acyltransferase Acyl-CoA ligase Beta-ketothiolase Fatty acid oxidation complex subunit beta |
Gene namesi | Name:fadI Synonyms:yfcY Ordered Locus Names:b2342, JW2339 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000206437 | 1 – 436 | 3-ketoacyl-CoA thiolase FadIAdd BLAST | 436 |
Proteomic databases
jPOSTi | P76503 |
PaxDbi | P76503 |
PRIDEi | P76503 |
Expressioni
Inductioni
Unlike the aerobic pathway, the anaerobic pathway is not strongly repressed by FadR regulatory protein.
Interactioni
Subunit structurei
Heterotetramer of two alpha chains (FadJ) and two beta chains (FadI).
By similarityProtein-protein interaction databases
BioGRIDi | 4260534, 173 interactors |
ComplexPortali | CPX-3966, fadJI fatty acid oxidation complex, anaerobic conditions |
DIPi | DIP-11991N |
IntActi | P76503, 12 interactors |
STRINGi | 511145.b2342 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0183, Bacteria |
HOGENOMi | CLU_031026_2_0_6 |
InParanoidi | P76503 |
PhylomeDBi | P76503 |
Family and domain databases
CDDi | cd00751, thiolase, 1 hit |
Gene3Di | 3.40.47.10, 1 hit |
HAMAPi | MF_01618, FadI, 1 hit |
InterProi | View protein in InterPro IPR012806, Ac-CoA_C-AcTrfase_FadI IPR002155, Thiolase IPR016039, Thiolase-like IPR020615, Thiolase_acyl_enz_int_AS IPR020610, Thiolase_AS IPR020617, Thiolase_C IPR020613, Thiolase_CS IPR020616, Thiolase_N |
Pfami | View protein in Pfam PF02803, Thiolase_C, 1 hit PF00108, Thiolase_N, 1 hit |
PIRSFi | PIRSF000429, Ac-CoA_Ac_transf, 1 hit |
SUPFAMi | SSF53901, SSF53901, 2 hits |
TIGRFAMsi | TIGR01930, AcCoA-C-Actrans, 1 hit TIGR02446, FadI, 1 hit |
PROSITEi | View protein in PROSITE PS00098, THIOLASE_1, 1 hit PS00737, THIOLASE_2, 1 hit PS00099, THIOLASE_3, 1 hit |
i Sequence
Sequence statusi: Complete.
P76503-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGQVLPLVTR QGDRIAIVSG LRTPFARQAT AFHGIPAVDL GKMVVGELLA
60 70 80 90 100
RSEIPAEVIE QLVFGQVVQM PEAPNIAREI VLGTGMNVHT DAYSVSRACA
110 120 130 140 150
TSFQAVANVA ESLMAGTIRA GIAGGADSSS VLPIGVSKKL ARVLVDVNKA
160 170 180 190 200
RTMSQRLKLF SRLRLRDLMP VPPAVAEYST GLRMGDTAEQ MAKTYGITRE
210 220 230 240 250
QQDALAHRSH QRAAQAWSDG KLKEEVMTAF IPPYKQPLVE DNNIRGNSSL
260 270 280 290 300
ADYAKLRPAF DRKHGTVTAA NSTPLTDGAA AVILMTESRA KELGLVPLGY
310 320 330 340 350
LRSYAFTAID VWQDMLLGPA WSTPLALERA GLTMSDLTLI DMHEAFAAQT
360 370 380 390 400
LANIQLLGSE RFAREALGRA HATGEVDDSK FNVLGGSIAY GHPFAATGAR
410 420 430
MITQTLHELR RRGGGFGLVT ACAAGGLGAA MVLEAE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC75402.1 AP009048 Genomic DNA Translation: BAA16197.2 |
PIRi | D65007 |
RefSeqi | NP_416844.1, NC_000913.3 WP_000531952.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC75402; AAC75402; b2342 BAA16197; BAA16197; BAA16197 |
GeneIDi | 948823 |
KEGGi | ecj:JW2339 eco:b2342 |
PATRICi | fig|1411691.4.peg.4390 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC75402.1 AP009048 Genomic DNA Translation: BAA16197.2 |
PIRi | D65007 |
RefSeqi | NP_416844.1, NC_000913.3 WP_000531952.1, NZ_LN832404.1 |
3D structure databases
SASBDBi | P76503 |
SMRi | P76503 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4260534, 173 interactors |
ComplexPortali | CPX-3966, fadJI fatty acid oxidation complex, anaerobic conditions |
DIPi | DIP-11991N |
IntActi | P76503, 12 interactors |
STRINGi | 511145.b2342 |
Proteomic databases
jPOSTi | P76503 |
PaxDbi | P76503 |
PRIDEi | P76503 |
Genome annotation databases
EnsemblBacteriai | AAC75402; AAC75402; b2342 BAA16197; BAA16197; BAA16197 |
GeneIDi | 948823 |
KEGGi | ecj:JW2339 eco:b2342 |
PATRICi | fig|1411691.4.peg.4390 |
Organism-specific databases
EchoBASEi | EB3880 |
Phylogenomic databases
eggNOGi | COG0183, Bacteria |
HOGENOMi | CLU_031026_2_0_6 |
InParanoidi | P76503 |
PhylomeDBi | P76503 |
Enzyme and pathway databases
UniPathwayi | UPA00659 |
BioCyci | EcoCyc:G7213-MONOMER MetaCyc:G7213-MONOMER |
Miscellaneous databases
PROi | PR:P76503 |
Family and domain databases
CDDi | cd00751, thiolase, 1 hit |
Gene3Di | 3.40.47.10, 1 hit |
HAMAPi | MF_01618, FadI, 1 hit |
InterProi | View protein in InterPro IPR012806, Ac-CoA_C-AcTrfase_FadI IPR002155, Thiolase IPR016039, Thiolase-like IPR020615, Thiolase_acyl_enz_int_AS IPR020610, Thiolase_AS IPR020617, Thiolase_C IPR020613, Thiolase_CS IPR020616, Thiolase_N |
Pfami | View protein in Pfam PF02803, Thiolase_C, 1 hit PF00108, Thiolase_N, 1 hit |
PIRSFi | PIRSF000429, Ac-CoA_Ac_transf, 1 hit |
SUPFAMi | SSF53901, SSF53901, 2 hits |
TIGRFAMsi | TIGR01930, AcCoA-C-Actrans, 1 hit TIGR02446, FadI, 1 hit |
PROSITEi | View protein in PROSITE PS00098, THIOLASE_1, 1 hit PS00737, THIOLASE_2, 1 hit PS00099, THIOLASE_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | FADI_ECOLI | |
Accessioni | P76503Primary (citable) accession number: P76503 Secondary accession number(s): P77208, P77230, Q6KCX1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1999 |
Last sequence update: | February 1, 1997 | |
Last modified: | February 10, 2021 | |
This is version 162 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families