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UniProtKB - P76472 (ARND_ECOLI)
Protein
Probable 4-deoxy-4-formamido-L-arabinose-phosphoundecaprenol deformylase ArnD
Gene
arnD
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the deformylation of 4-deoxy-4-formamido-L-arabinose-phosphoundecaprenol to 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol. The modified arabinose is attached to lipid A and is required for resistance to polymyxin and cationic antimicrobial peptides (Probable).
1 PublicationCatalytic activityi
- 4-deoxy-4-formamido-α-L-arabinopyranosyl di-trans,octa-cis-undecaprenyl phosphate + H2O = 4-amino-4-deoxy-α-L-arabinopyranosyl di-trans,octa-cis-undecaprenyl phosphate + formateUniRule annotation1 PublicationEC:3.5.1.n3UniRule annotation1 Publication
: 4-amino-4-deoxy-alpha-L-arabinose undecaprenyl phosphate biosynthesis Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes 4-amino-4-deoxy-alpha-L-arabinose undecaprenyl phosphate from UDP-4-deoxy-4-formamido-beta-L-arabinose and undecaprenyl phosphate.UniRule annotation1 Publication This subpathway is part of the pathway 4-amino-4-deoxy-alpha-L-arabinose undecaprenyl phosphate biosynthesis, which is itself part of Glycolipid biosynthesis.View all proteins of this organism that are known to be involved in the subpathway that synthesizes 4-amino-4-deoxy-alpha-L-arabinose undecaprenyl phosphate from UDP-4-deoxy-4-formamido-beta-L-arabinose and undecaprenyl phosphate, the pathway 4-amino-4-deoxy-alpha-L-arabinose undecaprenyl phosphate biosynthesis and in Glycolipid biosynthesis.
Pathwayi: lipopolysaccharide biosynthesis
This protein is involved in the pathway lipopolysaccharide biosynthesis, which is part of Bacterial outer membrane biogenesis.UniRule annotation1 PublicationView all proteins of this organism that are known to be involved in the pathway lipopolysaccharide biosynthesis and in Bacterial outer membrane biogenesis.
GO - Molecular functioni
- hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides Source: UniProtKB-UniRule
GO - Biological processi
- 4-amino-4-deoxy-alpha-L-arabinopyranosyl undecaprenyl phosphate biosynthetic process Source: UniProtKB-UniPathway
- lipid A biosynthetic process Source: UniProtKB-UniRule
- lipopolysaccharide biosynthetic process Source: UniProtKB-UniPathway
- response to antibiotic Source: EcoCyc
- response to iron(III) ion Source: EcoCyc
Keywordsi
Molecular function | Hydrolase |
Biological process | Antibiotic resistance, Lipid A biosynthesis, Lipid biosynthesis, Lipid metabolism, Lipopolysaccharide biosynthesis |
Enzyme and pathway databases
BioCyci | EcoCyc:G7169-MONOMER |
UniPathwayi | UPA00030 UPA00036;UER00496 |
Names & Taxonomyi
Protein namesi | Recommended name: Probable 4-deoxy-4-formamido-L-arabinose-phosphoundecaprenol deformylase ArnDUniRule annotation (EC:3.5.1.n3UniRule annotation) |
Gene namesi | Name:arnDUniRule annotation Synonyms:pmrJ, yfbH Ordered Locus Names:b2256, JW2250 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000169176 | 1 – 296 | Probable 4-deoxy-4-formamido-L-arabinose-phosphoundecaprenol deformylase ArnDAdd BLAST | 296 |
Proteomic databases
PaxDbi | P76472 |
PRIDEi | P76472 |
Expressioni
Inductioni
Induced by BasR.By similarity
Interactioni
Protein-protein interaction databases
BioGRIDi | 4260498, 208 interactors |
IntActi | P76472, 1 interactor |
STRINGi | 511145.b2256 |
Structurei
3D structure databases
AlphaFoldDBi | P76472 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 2 – 260 | NodB homologyUniRule annotationAdd BLAST | 259 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0726, Bacteria |
HOGENOMi | CLU_084199_0_0_6 |
InParanoidi | P76472 |
OMAi | KFLWKML |
PhylomeDBi | P76472 |
Family and domain databases
HAMAPi | MF_01870, ArnD, 1 hit |
InterProi | View protein in InterPro IPR023557, ArnD IPR011330, Glyco_hydro/deAcase_b/a-brl IPR002509, NODB_dom |
Pfami | View protein in Pfam PF01522, Polysacc_deac_1, 1 hit |
SUPFAMi | SSF88713, SSF88713, 1 hit |
PROSITEi | View protein in PROSITE PS51677, NODB, 1 hit |
i Sequence
Sequence statusi: Complete.
P76472-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTKVGLRIDV DTFRGTREGV PRLLEILSKH NIQASIFFSV GPDNMGRHLW
60 70 80 90 100
RLVKPQFLWK MLRSNAASLY GWDILLAGTA WPGKEIGHAN ADIIREAAKH
110 120 130 140 150
HEVGLHAWDH HAWQARSGNW DRQTMIDDIA RGLRTLEEII GQPVTCSAAA
160 170 180 190 200
GWRADQKVIE AKEAFHLRYN SDCRGAMPFR PLLESGNPGT AQIPVTLPTW
210 220 230 240 250
DEVIGRDVKA EDFNGWLLNR ILRDKGTPVY TIHAEVEGCA YQHNFVDLLK
260 270 280 290
RAAQEGVTFC PLSELLSETL PLGQVVRGNI AGREGWLGCQ QIAGSR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC75316.1 AP009048 Genomic DNA Translation: BAE76673.1 |
PIRi | F64996 |
RefSeqi | NP_416759.1, NC_000913.3 WP_000169728.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC75316; AAC75316; b2256 BAE76673; BAE76673; BAE76673 |
GeneIDi | 58389479 945334 |
KEGGi | ecj:JW2250 eco:b2256 |
PATRICi | fig|1411691.4.peg.4481 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC75316.1 AP009048 Genomic DNA Translation: BAE76673.1 |
PIRi | F64996 |
RefSeqi | NP_416759.1, NC_000913.3 WP_000169728.1, NZ_LN832404.1 |
3D structure databases
AlphaFoldDBi | P76472 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 4260498, 208 interactors |
IntActi | P76472, 1 interactor |
STRINGi | 511145.b2256 |
Proteomic databases
PaxDbi | P76472 |
PRIDEi | P76472 |
Genome annotation databases
EnsemblBacteriai | AAC75316; AAC75316; b2256 BAE76673; BAE76673; BAE76673 |
GeneIDi | 58389479 945334 |
KEGGi | ecj:JW2250 eco:b2256 |
PATRICi | fig|1411691.4.peg.4481 |
Organism-specific databases
EchoBASEi | EB3845 |
Phylogenomic databases
eggNOGi | COG0726, Bacteria |
HOGENOMi | CLU_084199_0_0_6 |
InParanoidi | P76472 |
OMAi | KFLWKML |
PhylomeDBi | P76472 |
Enzyme and pathway databases
UniPathwayi | UPA00030 UPA00036;UER00496 |
BioCyci | EcoCyc:G7169-MONOMER |
Miscellaneous databases
PROi | PR:P76472 |
Family and domain databases
HAMAPi | MF_01870, ArnD, 1 hit |
InterProi | View protein in InterPro IPR023557, ArnD IPR011330, Glyco_hydro/deAcase_b/a-brl IPR002509, NODB_dom |
Pfami | View protein in Pfam PF01522, Polysacc_deac_1, 1 hit |
SUPFAMi | SSF88713, SSF88713, 1 hit |
PROSITEi | View protein in PROSITE PS51677, NODB, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ARND_ECOLI | |
Accessioni | P76472Primary (citable) accession number: P76472 Secondary accession number(s): Q2MAN3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 27, 2001 |
Last sequence update: | February 1, 1997 | |
Last modified: | May 25, 2022 | |
This is version 139 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families