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Protein

Acetyl-CoA acetyltransferase

Gene

atoB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

2 acetyl-CoA = CoA + acetoacetyl-CoA.PROSITE-ProRule annotation

Pathwayi: (R)-mevalonate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes (R)-mevalonate from acetyl-CoA.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Acetyl-CoA acetyltransferase (atoB)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
This subpathway is part of the pathway (R)-mevalonate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-mevalonate from acetyl-CoA, the pathway (R)-mevalonate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei88Acyl-thioester intermediateBy similarity1
Active sitei349Proton acceptorPROSITE-ProRule annotation1
Active sitei379Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • acetoacetic acid catabolic process Source: EcoCyc
  • fatty acid beta-oxidation Source: GO_Central
  • protein homotetramerization Source: EcoCyc

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processFatty acid metabolism, Lipid metabolism

Enzyme and pathway databases

BioCyciEcoCyc:ACETYL-COA-ACETYLTRANSFER-MONOMER
MetaCyc:ACETYL-COA-ACETYLTRANSFER-MONOMER
UniPathwayi
UPA00058;UER00101

Names & Taxonomyi

Protein namesi
Recommended name:
Acetyl-CoA acetyltransferase (EC:2.3.1.9)
Alternative name(s):
Acetoacetyl-CoA thiolase
Gene namesi
Name:atoB
Ordered Locus Names:b2224, JW2218
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11672 atoB

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002064061 – 394Acetyl-CoA acetyltransferaseAdd BLAST394

Proteomic databases

PaxDbiP76461
PRIDEiP76461

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi4263266, 10 interactors
IntActiP76461, 3 interactors
STRINGi316385.ECDH10B_2382

Structurei

Secondary structure

1394
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP76461
SMRiP76461
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thiolase family.Curated

Phylogenomic databases

eggNOGiENOG4105CHU Bacteria
COG0183 LUCA
HOGENOMiHOG000012240
InParanoidiP76461
KOiK00626
OMAiFDNELIP
PhylomeDBiP76461

Family and domain databases

CDDicd00751 thiolase, 1 hit
Gene3Di3.40.47.10, 4 hits
InterProiView protein in InterPro
IPR002155 Thiolase
IPR016039 Thiolase-like
IPR020615 Thiolase_acyl_enz_int_AS
IPR020610 Thiolase_AS
IPR020617 Thiolase_C
IPR020613 Thiolase_CS
IPR020616 Thiolase_N
PfamiView protein in Pfam
PF02803 Thiolase_C, 1 hit
PF00108 Thiolase_N, 1 hit
PIRSFiPIRSF000429 Ac-CoA_Ac_transf, 1 hit
SUPFAMiSSF53901 SSF53901, 2 hits
TIGRFAMsiTIGR01930 AcCoA-C-Actrans, 1 hit
PROSITEiView protein in PROSITE
PS00098 THIOLASE_1, 1 hit
PS00737 THIOLASE_2, 1 hit
PS00099 THIOLASE_3, 1 hit

Sequencei

Sequence statusi: Complete.

P76461-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKNCVIVSAV RTAIGSFNGS LASTSAIDLG ATVIKAAIER AKIDSQHVDE
60 70 80 90 100
VIMGNVLQAG LGQNPARQAL LKSGLAETVC GFTVNKVCGS GLKSVALAAQ
110 120 130 140 150
AIQAGQAQSI VAGGMENMSL APYLLDAKAR SGYRLGDGQV YDVILRDGLM
160 170 180 190 200
CATHGYHMGI TAENVAKEYG ITREMQDELA LHSQRKAAAA IESGAFTAEI
210 220 230 240 250
VPVNVVTRKK TFVFSQDEFP KANSTAEALG ALRPAFDKAG TVTAGNASGI
260 270 280 290 300
NDGAAALVIM EESAALAAGL TPLARIKSYA SGGVPPALMG MGPVPATQKA
310 320 330 340 350
LQLAGLQLAD IDLIEANEAF AAQFLAVGKN LGFDSEKVNV NGGAIALGHP
360 370 380 390
IGASGARILV TLLHAMQARD KTLGLATLCI GGGQGIAMVI ERLN
Length:394
Mass (Da):40,352
Last modified:February 1, 1997 - v1
Checksum:iDE27C394C3E6BDFA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC75284.1
AP009048 Genomic DNA Translation: BAA16020.1
PIRiF64992
RefSeqiNP_416728.1, NC_000913.3
WP_000786547.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75284; AAC75284; b2224
BAA16020; BAA16020; BAA16020
GeneIDi946727
KEGGiecj:JW2218
eco:b2224
PATRICifig|1411691.4.peg.11

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC75284.1
AP009048 Genomic DNA Translation: BAA16020.1
PIRiF64992
RefSeqiNP_416728.1, NC_000913.3
WP_000786547.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4WYSX-ray2.10A/B/C/D1-393[»]
5F0VX-ray1.80A/B/C/D1-393[»]
5F38X-ray1.90A1-394[»]
B/C1-393[»]
D1-392[»]
ProteinModelPortaliP76461
SMRiP76461
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263266, 10 interactors
IntActiP76461, 3 interactors
STRINGi316385.ECDH10B_2382

Proteomic databases

PaxDbiP76461
PRIDEiP76461

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75284; AAC75284; b2224
BAA16020; BAA16020; BAA16020
GeneIDi946727
KEGGiecj:JW2218
eco:b2224
PATRICifig|1411691.4.peg.11

Organism-specific databases

EchoBASEiEB1623
EcoGeneiEG11672 atoB

Phylogenomic databases

eggNOGiENOG4105CHU Bacteria
COG0183 LUCA
HOGENOMiHOG000012240
InParanoidiP76461
KOiK00626
OMAiFDNELIP
PhylomeDBiP76461

Enzyme and pathway databases

UniPathwayi
UPA00058;UER00101

BioCyciEcoCyc:ACETYL-COA-ACETYLTRANSFER-MONOMER
MetaCyc:ACETYL-COA-ACETYLTRANSFER-MONOMER

Miscellaneous databases

PROiPR:P76461

Family and domain databases

CDDicd00751 thiolase, 1 hit
Gene3Di3.40.47.10, 4 hits
InterProiView protein in InterPro
IPR002155 Thiolase
IPR016039 Thiolase-like
IPR020615 Thiolase_acyl_enz_int_AS
IPR020610 Thiolase_AS
IPR020617 Thiolase_C
IPR020613 Thiolase_CS
IPR020616 Thiolase_N
PfamiView protein in Pfam
PF02803 Thiolase_C, 1 hit
PF00108 Thiolase_N, 1 hit
PIRSFiPIRSF000429 Ac-CoA_Ac_transf, 1 hit
SUPFAMiSSF53901 SSF53901, 2 hits
TIGRFAMsiTIGR01930 AcCoA-C-Actrans, 1 hit
PROSITEiView protein in PROSITE
PS00098 THIOLASE_1, 1 hit
PS00737 THIOLASE_2, 1 hit
PS00099 THIOLASE_3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiATOB_ECOLI
AccessioniPrimary (citable) accession number: P76461
Secondary accession number(s): P78176
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: March 28, 2018
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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