The new UniProt website is here!
Take me to UniProt BETA
UniProtKB - P76446 (PDEN_ECOLI)
Protein
Probable cyclic di-GMP phosphodiesterase PdeN
Gene
pdeN
Organism
Escherichia coli (strain K12)
Status
Functioni
Phosphodiesterase (PDE) that catalyzes the hydrolysis of cyclic-di-GMP (c-di-GMP) to 5'-pGpG.
By similarityCaution
Was originally (Ref. 4) thought to originate from Proteus vulgaris, but was shown to originate from E.coli.1 Publication
Catalytic activityi
- EC:3.1.4.52By similarity
GO - Molecular functioni
- cyclic-guanylate-specific phosphodiesterase activity Source: UniProtKB-EC
Keywordsi
Molecular function | Hydrolase |
Ligand | c-di-GMP |
Enzyme and pathway databases
BioCyci | EcoCyc:G7148-MONOMER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:pdeN1 Publication Synonyms:rtn Ordered Locus Names:b2176, JW2164 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane 1 Publication; Multi-pass membrane protein Sequence analysis
Plasma Membrane
- plasma membrane Source: EcoCyc
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 16 – 36 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 236 – 256 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000097517 | 1 – 518 | Probable cyclic di-GMP phosphodiesterase PdeNAdd BLAST | 518 |
Proteomic databases
PaxDbi | P76446 |
PRIDEi | P76446 |
Expressioni
Inductioni
Expressed at both 28 and 37 degrees Celsius during exponential growth. Repressed by RpoS.1 Publication
Interactioni
Protein-protein interaction databases
BioGRIDi | 4261095, 10 interactors |
DIPi | DIP-10813N |
STRINGi | 511145.b2176 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 261 – 514 | EALPROSITE-ProRule annotationAdd BLAST | 254 |
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG2200, Bacteria |
HOGENOMi | CLU_000445_131_2_6 |
InParanoidi | P76446 |
OMAi | FLQGYWI |
PhylomeDBi | P76446 |
Family and domain databases
CDDi | cd01948, EAL, 1 hit |
Gene3Di | 3.20.20.450, 1 hit |
InterProi | View protein in InterPro IPR024744, CSS-motif_dom IPR001633, EAL_dom IPR035919, EAL_sf |
Pfami | View protein in Pfam PF12792, CSS-motif, 1 hit PF00563, EAL, 1 hit |
SMARTi | View protein in SMART SM00052, EAL, 1 hit |
SUPFAMi | SSF141868, SSF141868, 1 hit |
PROSITEi | View protein in PROSITE PS50883, EAL, 1 hit |
i Sequence
Sequence statusi: Complete.
P76446-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MFIRAPNFGR KLLLTCIVAG VMIAILVSCL QFLVAWHKHE VKYDTLITDV
60 70 80 90 100
QKYLDTYFAD LKSTTDRLQP LTLDTCQQAN PELTARAAFS MNVRTFVLVK
110 120 130 140 150
DKKTFCSSAT GEMDIPLNEL IPALDINKNV DMAILPGTPM VPNKPAIVIW
160 170 180 190 200
YRNPLLKNSG VFAALNLNLT PSLFYSSRQE DYDGVALIIG NTALSTFSSR
210 220 230 240 250
LMNVNELTDM PVRETKIAGI PLTVRLYADD WTWNDVWYAF LLGGMSGTVV
260 270 280 290 300
GLLCYYLMSV RMRPGREIMT AIKREQFYVA YQPVVDTQAL RVTGLEVLLR
310 320 330 340 350
WRHPVAGEIP PDAFINFAES QKMIVPLTQH LFELIARDAA ELEKVLPVGV
360 370 380 390 400
KFGINIAPDH LHSESFKADI QKLLTSLPAH HFQIVLEITE RDMLKEQEAT
410 420 430 440 450
QLFAWLHSVG VEIAIDDFGT GHSALIYLER FTLDYLKIDR GFINAIGTET
460 470 480 490 500
ITSPVLDAVL TLAKRLNMLT VAEGVETPEQ ARWLSERGVN FMQGYWISRP
510
LPLDDFVRWL KKPYTPQW
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 482 – 518 | RWLSE…YTPQW → D no nucleotide entry (Ref. 4) CuratedAdd BLAST | 37 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC75237.1 AP009048 Genomic DNA Translation: BAA15984.1 U12780 Genomic DNA Translation: AAA50754.1 U83404 Genomic DNA Translation: AAB51508.1 |
PIRi | G64986 |
RefSeqi | NP_416681.1, NC_000913.3 WP_000470560.1, NZ_SSZK01000027.1 |
Genome annotation databases
EnsemblBacteriai | AAC75237; AAC75237; b2176 BAA15984; BAA15984; BAA15984 |
GeneIDi | 946695 |
KEGGi | ecj:JW2164 eco:b2176 |
PATRICi | fig|1411691.4.peg.60 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC75237.1 AP009048 Genomic DNA Translation: BAA15984.1 U12780 Genomic DNA Translation: AAA50754.1 U83404 Genomic DNA Translation: AAB51508.1 |
PIRi | G64986 |
RefSeqi | NP_416681.1, NC_000913.3 WP_000470560.1, NZ_SSZK01000027.1 |
3D structure databases
AlphaFoldDBi | P76446 |
SMRi | P76446 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4261095, 10 interactors |
DIPi | DIP-10813N |
STRINGi | 511145.b2176 |
Proteomic databases
PaxDbi | P76446 |
PRIDEi | P76446 |
Genome annotation databases
EnsemblBacteriai | AAC75237; AAC75237; b2176 BAA15984; BAA15984; BAA15984 |
GeneIDi | 946695 |
KEGGi | ecj:JW2164 eco:b2176 |
PATRICi | fig|1411691.4.peg.60 |
Organism-specific databases
EchoBASEi | EB3830 |
Phylogenomic databases
eggNOGi | COG2200, Bacteria |
HOGENOMi | CLU_000445_131_2_6 |
InParanoidi | P76446 |
OMAi | FLQGYWI |
PhylomeDBi | P76446 |
Enzyme and pathway databases
BioCyci | EcoCyc:G7148-MONOMER |
Miscellaneous databases
PROi | PR:P76446 |
Family and domain databases
CDDi | cd01948, EAL, 1 hit |
Gene3Di | 3.20.20.450, 1 hit |
InterProi | View protein in InterPro IPR024744, CSS-motif_dom IPR001633, EAL_dom IPR035919, EAL_sf |
Pfami | View protein in Pfam PF12792, CSS-motif, 1 hit PF00563, EAL, 1 hit |
SMARTi | View protein in SMART SM00052, EAL, 1 hit |
SUPFAMi | SSF141868, SSF141868, 1 hit |
PROSITEi | View protein in PROSITE PS50883, EAL, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PDEN_ECOLI | |
Accessioni | P76446Primary (citable) accession number: P76446 Secondary accession number(s): P77139, Q52613 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | February 1, 1997 | |
Last modified: | May 25, 2022 | |
This is version 139 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |