UniProtKB - P76445 (LPXT_ECOLI)
Protein
Lipid A 1-diphosphate synthase
Gene
lpxT
Organism
Escherichia coli (strain K12)
Status
Functioni
Involved in the modification of the lipid A domain of lipopolysaccharides (LPS). Transfers a phosphate group from undecaprenyl pyrophosphate (C55-PP) to lipid A to form lipid A 1-diphosphate. Contributes to the recycling of undecaprenyl phosphate (C55-P) (PubMed:18047581). In vitro, has low undecaprenyl-diphosphate phosphatase activity (PubMed:17660416).2 Publications
Miscellaneous
In E.coli, lipid A contains typically a monophosphate unit at positions 1 and 4' (bis-phosphorylated lipid A). However, one-third of the lipid A contains an unsubstituted diphosphate unit at position 1 (lipid A 1-diphosphate).1 Publication
Catalytic activityi
- α-Kdo-(2→4)-α-Kdo-(2→6)-lipid A + di-trans,octa-cis-undecaprenyl diphosphate = (Kdo)2-lipid A 1-diphosphate + di-trans,octa-cis-undecaprenyl phosphateUniRule annotation1 PublicationEC:2.7.4.29UniRule annotation1 Publication
Activity regulationi
Inhibited by BasR. This regulation does not occur at the level of transcription, but rather following the assembly of LpxT into the inner membrane.1 Publication
pH dependencei
Optimum pH is 7.0. Significant activity is observed from pH 5.5 to pH 7.5.1 Publication
: lipopolysaccharide biosynthesis Pathwayi
This protein is involved in the pathway lipopolysaccharide biosynthesis, which is part of Bacterial outer membrane biogenesis.UniRule annotation1 PublicationView all proteins of this organism that are known to be involved in the pathway lipopolysaccharide biosynthesis and in Bacterial outer membrane biogenesis.
GO - Molecular functioni
- phosphotransferase activity, phosphate group as acceptor Source: EcoCyc
- undecaprenyl-diphosphatase activity Source: EcoCyc
GO - Biological processi
- Gram-negative-bacterium-type cell outer membrane assembly Source: EcoCyc
- lipid A biosynthetic process Source: UniProtKB-UniRule
- lipopolysaccharide biosynthetic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Transferase |
Biological process | Lipopolysaccharide biosynthesis |
Enzyme and pathway databases
BioCyci | EcoCyc:G7146-MONOMER MetaCyc:G7146-MONOMER |
UniPathwayi | UPA00030 |
Names & Taxonomyi
Protein namesi | Recommended name: Lipid A 1-diphosphate synthaseUniRule annotationCurated (EC:2.7.4.29UniRule annotation1 Publication)Alternative name(s): Kdo(2)-lipid A phosphotransferaseCurated Undecaprenyl pyrophosphate:lipid A 1-phosphate phosphotransferaseUniRule annotationCurated |
Gene namesi | Name:lpxT1 PublicationUniRule annotation Synonyms:yeiU Ordered Locus Names:b2174, JW2162 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane UniRule annotation2 Publications; Multi-pass membrane protein UniRule annotation1 Publication
Note: Transferase activity takes place on the periplamic side of the inner membrane.UniRule annotation1 Publication
Plasma Membrane
- integral component of plasma membrane Source: UniProtKB-UniRule
- plasma membrane Source: EcoCyc
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 5 | Cytoplasmic1 Publication | 5 | |
Transmembranei | 6 – 26 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 27 – 62 | Periplasmic1 PublicationAdd BLAST | 36 | |
Transmembranei | 63 – 83 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 84 – 90 | Cytoplasmic1 Publication | 7 | |
Transmembranei | 91 – 111 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 112 – 145 | Periplasmic1 PublicationAdd BLAST | 34 | |
Topological domaini | 167 | Cytoplasmic1 Publication | 1 | |
Transmembranei | 168 – 188 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 189 – 194 | Periplasmic1 Publication | 6 | |
Transmembranei | 195 – 215 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 216 – 237 | Cytoplasmic2 PublicationsAdd BLAST | 22 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Deletion of the gene results in the production of LPS containing only the bis-phosphorylated lipid A species.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 190 | H → A: Lack of activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000169160 | 1 – 237 | Lipid A 1-diphosphate synthaseAdd BLAST | 237 |
Proteomic databases
jPOSTi | P76445 |
PaxDbi | P76445 |
PRIDEi | P76445 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4260460, 4 interactors |
STRINGi | 511145.b2174 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG0671, Bacteria |
HOGENOMi | CLU_102925_0_0_6 |
InParanoidi | P76445 |
PhylomeDBi | P76445 |
Family and domain databases
HAMAPi | MF_01945, Lipid_A_LpxT, 1 hit |
InterProi | View protein in InterPro IPR032908, LpxT IPR036938, P_Acid_Pase_2/haloperoxi_sf IPR000326, P_Acid_Pase_2/haloperoxidase |
Pfami | View protein in Pfam PF01569, PAP2, 1 hit |
SMARTi | View protein in SMART SM00014, acidPPc, 1 hit |
SUPFAMi | SSF48317, SSF48317, 1 hit |
i Sequence
Sequence statusi: Complete.
P76445-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIKNLPQIVL LNIVGLALFL SWYIPVNHGF WLPIDADIFY FFNQKLVESK
60 70 80 90 100
AFLWLVALTN NRAFDGCSLL AMGMLMLSFW LKENAPGRRR IVIIGLVMLL
110 120 130 140 150
TAVVLNQLGQ ALIPVKRASP TLTFTDINRV SELLSVPTKD ASRDSFPGDH
160 170 180 190 200
GMMLLIFSAF MWRYFGKVAG LIALIIFVVF AFPRVMIGAH WFTDIIVGSM
210 220 230
TVILIGLPWV LLTPLSDRLI TFFDKSLPGK NKHFQNK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC75235.2 AP009048 Genomic DNA Translation: BAE76645.1 |
RefSeqi | NP_416679.4, NC_000913.3 WP_000594599.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC75235; AAC75235; b2174 BAE76645; BAE76645; BAE76645 |
GeneIDi | 57729687 946693 |
KEGGi | ecj:JW2162 eco:b2174 |
PATRICi | fig|1411691.4.peg.62 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC75235.2 AP009048 Genomic DNA Translation: BAE76645.1 |
RefSeqi | NP_416679.4, NC_000913.3 WP_000594599.1, NZ_LN832404.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 4260460, 4 interactors |
STRINGi | 511145.b2174 |
Proteomic databases
jPOSTi | P76445 |
PaxDbi | P76445 |
PRIDEi | P76445 |
Genome annotation databases
EnsemblBacteriai | AAC75235; AAC75235; b2174 BAE76645; BAE76645; BAE76645 |
GeneIDi | 57729687 946693 |
KEGGi | ecj:JW2162 eco:b2174 |
PATRICi | fig|1411691.4.peg.62 |
Organism-specific databases
EchoBASEi | EB3828 |
Phylogenomic databases
eggNOGi | COG0671, Bacteria |
HOGENOMi | CLU_102925_0_0_6 |
InParanoidi | P76445 |
PhylomeDBi | P76445 |
Enzyme and pathway databases
UniPathwayi | UPA00030 |
BioCyci | EcoCyc:G7146-MONOMER MetaCyc:G7146-MONOMER |
Miscellaneous databases
PROi | PR:P76445 |
Family and domain databases
HAMAPi | MF_01945, Lipid_A_LpxT, 1 hit |
InterProi | View protein in InterPro IPR032908, LpxT IPR036938, P_Acid_Pase_2/haloperoxi_sf IPR000326, P_Acid_Pase_2/haloperoxidase |
Pfami | View protein in Pfam PF01569, PAP2, 1 hit |
SMARTi | View protein in SMART SM00014, acidPPc, 1 hit |
SUPFAMi | SSF48317, SSF48317, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LPXT_ECOLI | |
Accessioni | P76445Primary (citable) accession number: P76445 Secondary accession number(s): Q2MAR1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | July 15, 1998 | |
Last modified: | February 10, 2021 | |
This is version 132 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families