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UniProtKB - P76403 (TRHP_ECOLI)

Entry version 124 (07 Apr 2021)
Sequence version 1 (01 Feb 1997)
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Protein

tRNA hydroxylation protein P

Gene

trhP

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Involved in prephenate-dependent formation of 5-hydroxyuridine (ho5U) modification at position 34 in tRNAs, the first step in 5-carboxymethoxyuridine (cmo5U) biosynthesis (PubMed:31253794). Involved differently in ho5U formation in each tRNA; tRNA(Leu3) and tRNA(Pro3) are major targets of TrhP (PubMed:31253794).1 Publication

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

  • tRNA modification Source: UniProtKB
  • tRNA wobble uridine modification Source: EcoCyc
Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionHydrolase, Protease
Biological processtRNA processing

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...BioCyci		EcoCyc:G7118-MONOMER

Protein family/group databases

MEROPS protease database

More...MEROPSi		U32.002

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
tRNA hydroxylation protein P1 Publication (EC:3.4.-.-Curated)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:trhP1 Publication
Synonyms:yegQ
Ordered Locus Names:b2081, JW2066
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia ›
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the 'Pathology and Biotech' section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

Deletion mutant cells display reduced 5-methoxycarbonylmethoxyuridine (mcmo5U) formation in tRNA(Ala1), tRNA(Ser1) and tRNA(Thr4) (PubMed:31253794). Reduced 5-carboxymethoxyuridine (cmo5U) formation in tRNA(Val1) (PubMed:31253794). Strongly reduced cmo5U formation in tRNA(Leu3) and tRNA(Pro3) (PubMed:31253794). Cells lacking both trhP and trhO show complete absence of 5-carboxymethoxyuridine (cmo5U) modification in tRNAs; cells display a temperature-sensitive phenotype and decode codons ending in G (GCG and UCG) less efficiently than the wild-type strain (PubMed:31253794).1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi162E → A: Reduced 5-carboxymethoxyuridine formation in tRNAs. 1 Publication1
Mutagenesisi166H → A: Reduced 5-carboxymethoxyuridine formation in tRNAs. 1 Publication1
Mutagenesisi170C → A: Reduced 5-carboxymethoxyuridine formation in tRNAs. 1 Publication1
Mutagenesisi177C → A: Reduced 5-carboxymethoxyuridine formation in tRNAs. 1 Publication1
Mutagenesisi193C → A: Reduced 5-carboxymethoxyuridine formation in tRNAs. 1 Publication1
Mutagenesisi197C → A: Reduced 5-carboxymethoxyuridine formation in tRNAs. 1 Publication1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000791841 – 453tRNA hydroxylation protein PAdd BLAST453

Proteomic databases

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		P76403

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		P76403

PRoteomics IDEntifications database

More...PRIDEi		P76403

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		4263508, 43 interactors

Database of interacting proteins

More...DIPi		DIP-11887N

Protein interaction database and analysis system

More...IntActi		P76403, 8 interactors

STRING: functional protein association networks

More...STRINGi		511145.b2081

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the peptidase U32 family.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		COG0826, Bacteria

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_011540_0_2_6

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		P76403

Database for complete collections of gene phylogenies

More...PhylomeDBi		P76403

Family and domain databases

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR001539, Peptidase_U32
IPR032525, Peptidase_U32_C

Pfam protein domain database

More...Pfami		View protein in Pfam
PF01136, Peptidase_U32, 1 hit
PF16325, Peptidase_U32_C, 1 hit

PROSITE; a protein domain and family database

More...PROSITEi		View protein in PROSITE
PS01276, PEPTIDASE_U32, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P76403-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MFKPELLSPA GTLKNMRYAF AYGADAVYAG QPRYSLRVRN NEFNHENLQL 
        60         70         80         90        100
GINEAHALGK KFYVVVNIAP HNAKLKTFIR DLKPVVEMGP DALIMSDPGL 
       110        120        130        140        150
IMLVREHFPE MPIHLSVQAN AVNWATVKFW QQMGLTRVIL SRELSLEEIE 
       160        170        180        190        200
EIRNQVPDME IEIFVHGALC MAYSGRCLLS GYINKRDPNQ GTCTNACRWE 
       210        220        230        240        250
YNVQEGKEDD VGNIVHKYEP IPVQNVEPTL GIGAPTDKVF MIEEAQRPGE 
       260        270        280        290        300
YMTAFEDEHG TYIMNSKDLR AIAHVERLTK MGVHSLKIEG RTKSFYYCAR 
       310        320        330        340        350
TAQVYRKAID DAAAGKPFDT SLLETLEGLA HRGYTEGFLR RHTHDDYQNY 
       360        370        380        390        400
EYGYSVSDRQ QFVGEFTGER KGDLAAVAVK NKFSVGDSLE LMTPQGNINF 
       410        420        430        440        450
TLEHMENAKG EAMPIAPGDG YTVWLPVPQD LELNYALLMR NFSGETTRNP 

HGK
Length:453
Mass (Da):51,193
Last modified:February 1, 1997 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i1843AB6D040ECDDF
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC75142.1
AP009048 Genomic DNA Translation: BAA15936.1

Protein sequence database of the Protein Information Resource

More...PIRi		H64974

NCBI Reference Sequences

More...RefSeqi		NP_416585.1, NC_000913.3
WP_000476011.1, NZ_STEB01000002.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...EnsemblBacteriai		AAC75142; AAC75142; b2081
BAA15936; BAA15936; BAA15936

Database of genes from NCBI RefSeq genomes

More...GeneIDi		946609

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		ecj:JW2066
eco:b2081

Pathosystems Resource Integration Center (PATRIC)

More...PATRICi		fig|511145.12.peg.2159

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC75142.1
AP009048 Genomic DNA Translation: BAA15936.1
PIRiH64974
RefSeqiNP_416585.1, NC_000913.3
WP_000476011.1, NZ_STEB01000002.1

3D structure databases

Database of comparative protein structure models

More...ModBasei		Search...

SWISS-MODEL Interactive Workspace

More...SWISS-MODEL-Workspacei		Submit a new modelling project...

Protein-protein interaction databases

BioGRIDi4263508, 43 interactors
DIPiDIP-11887N
IntActiP76403, 8 interactors
STRINGi511145.b2081

Protein family/group databases

MEROPSiU32.002

Proteomic databases

jPOSTiP76403
PaxDbiP76403
PRIDEiP76403

Genome annotation databases

EnsemblBacteriaiAAC75142; AAC75142; b2081
BAA15936; BAA15936; BAA15936
GeneIDi946609
KEGGiecj:JW2066
eco:b2081
PATRICifig|511145.12.peg.2159

Organism-specific databases

EchoBASE - an integrated post-genomic database for E. coli

More...EchoBASEi		EB3813

Phylogenomic databases

eggNOGiCOG0826, Bacteria
HOGENOMiCLU_011540_0_2_6
InParanoidiP76403
PhylomeDBiP76403

Enzyme and pathway databases

BioCyciEcoCyc:G7118-MONOMER

Miscellaneous databases

Protein Ontology

More...PROi		PR:P76403

Family and domain databases

InterProiView protein in InterPro
IPR001539, Peptidase_U32
IPR032525, Peptidase_U32_C
PfamiView protein in Pfam
PF01136, Peptidase_U32, 1 hit
PF16325, Peptidase_U32_C, 1 hit
PROSITEiView protein in PROSITE
PS01276, PEPTIDASE_U32, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...ProtoNeti		Search...

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiTRHP_ECOLI
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P76403
Secondary accession number(s): O08007, O08010
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: April 7, 2021
This is version 124 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
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