UniProtKB - P76403 (TRHP_ECOLI)
Protein
tRNA hydroxylation protein P
Gene
trhP
Organism
Escherichia coli (strain K12)
Status
Functioni
Involved in prephenate-dependent formation of 5-hydroxyuridine (ho5U) modification at position 34 in tRNAs, the first step in 5-carboxymethoxyuridine (cmo5U) biosynthesis (PubMed:31253794). Involved differently in ho5U formation in each tRNA; tRNA(Leu3) and tRNA(Pro3) are major targets of TrhP (PubMed:31253794).1 Publication
GO - Molecular functioni
- peptidase activity Source: UniProtKB-KW
GO - Biological processi
- tRNA modification Source: UniProtKB
- tRNA wobble uridine modification Source: EcoCyc
Keywordsi
Molecular function | Hydrolase, Protease |
Biological process | tRNA processing |
Enzyme and pathway databases
BioCyci | EcoCyc:G7118-MONOMER |
Protein family/group databases
MEROPSi | U32.002 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:trhP1 Publication Synonyms:yegQ Ordered Locus Names:b2081, JW2066 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Deletion mutant cells display reduced 5-methoxycarbonylmethoxyuridine (mcmo5U) formation in tRNA(Ala1), tRNA(Ser1) and tRNA(Thr4) (PubMed:31253794). Reduced 5-carboxymethoxyuridine (cmo5U) formation in tRNA(Val1) (PubMed:31253794). Strongly reduced cmo5U formation in tRNA(Leu3) and tRNA(Pro3) (PubMed:31253794). Cells lacking both trhP and trhO show complete absence of 5-carboxymethoxyuridine (cmo5U) modification in tRNAs; cells display a temperature-sensitive phenotype and decode codons ending in G (GCG and UCG) less efficiently than the wild-type strain (PubMed:31253794).1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 162 | E → A: Reduced 5-carboxymethoxyuridine formation in tRNAs. 1 Publication | 1 | |
Mutagenesisi | 166 | H → A: Reduced 5-carboxymethoxyuridine formation in tRNAs. 1 Publication | 1 | |
Mutagenesisi | 170 | C → A: Reduced 5-carboxymethoxyuridine formation in tRNAs. 1 Publication | 1 | |
Mutagenesisi | 177 | C → A: Reduced 5-carboxymethoxyuridine formation in tRNAs. 1 Publication | 1 | |
Mutagenesisi | 193 | C → A: Reduced 5-carboxymethoxyuridine formation in tRNAs. 1 Publication | 1 | |
Mutagenesisi | 197 | C → A: Reduced 5-carboxymethoxyuridine formation in tRNAs. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000079184 | 1 – 453 | tRNA hydroxylation protein PAdd BLAST | 453 |
Proteomic databases
jPOSTi | P76403 |
PaxDbi | P76403 |
PRIDEi | P76403 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4263508, 43 interactors |
DIPi | DIP-11887N |
IntActi | P76403, 8 interactors |
STRINGi | 511145.b2081 |
Family & Domainsi
Sequence similaritiesi
Belongs to the peptidase U32 family.Curated
Phylogenomic databases
eggNOGi | COG0826, Bacteria |
HOGENOMi | CLU_011540_0_2_6 |
InParanoidi | P76403 |
PhylomeDBi | P76403 |
Family and domain databases
InterProi | View protein in InterPro IPR001539, Peptidase_U32 IPR032525, Peptidase_U32_C |
Pfami | View protein in Pfam PF01136, Peptidase_U32, 1 hit PF16325, Peptidase_U32_C, 1 hit |
PROSITEi | View protein in PROSITE PS01276, PEPTIDASE_U32, 1 hit |
i Sequence
Sequence statusi: Complete.
P76403-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MFKPELLSPA GTLKNMRYAF AYGADAVYAG QPRYSLRVRN NEFNHENLQL
60 70 80 90 100
GINEAHALGK KFYVVVNIAP HNAKLKTFIR DLKPVVEMGP DALIMSDPGL
110 120 130 140 150
IMLVREHFPE MPIHLSVQAN AVNWATVKFW QQMGLTRVIL SRELSLEEIE
160 170 180 190 200
EIRNQVPDME IEIFVHGALC MAYSGRCLLS GYINKRDPNQ GTCTNACRWE
210 220 230 240 250
YNVQEGKEDD VGNIVHKYEP IPVQNVEPTL GIGAPTDKVF MIEEAQRPGE
260 270 280 290 300
YMTAFEDEHG TYIMNSKDLR AIAHVERLTK MGVHSLKIEG RTKSFYYCAR
310 320 330 340 350
TAQVYRKAID DAAAGKPFDT SLLETLEGLA HRGYTEGFLR RHTHDDYQNY
360 370 380 390 400
EYGYSVSDRQ QFVGEFTGER KGDLAAVAVK NKFSVGDSLE LMTPQGNINF
410 420 430 440 450
TLEHMENAKG EAMPIAPGDG YTVWLPVPQD LELNYALLMR NFSGETTRNP
HGK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC75142.1 AP009048 Genomic DNA Translation: BAA15936.1 |
PIRi | H64974 |
RefSeqi | NP_416585.1, NC_000913.3 WP_000476011.1, NZ_STEB01000002.1 |
Genome annotation databases
EnsemblBacteriai | AAC75142; AAC75142; b2081 BAA15936; BAA15936; BAA15936 |
GeneIDi | 946609 |
KEGGi | ecj:JW2066 eco:b2081 |
PATRICi | fig|511145.12.peg.2159 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC75142.1 AP009048 Genomic DNA Translation: BAA15936.1 |
PIRi | H64974 |
RefSeqi | NP_416585.1, NC_000913.3 WP_000476011.1, NZ_STEB01000002.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 4263508, 43 interactors |
DIPi | DIP-11887N |
IntActi | P76403, 8 interactors |
STRINGi | 511145.b2081 |
Protein family/group databases
MEROPSi | U32.002 |
Proteomic databases
jPOSTi | P76403 |
PaxDbi | P76403 |
PRIDEi | P76403 |
Genome annotation databases
EnsemblBacteriai | AAC75142; AAC75142; b2081 BAA15936; BAA15936; BAA15936 |
GeneIDi | 946609 |
KEGGi | ecj:JW2066 eco:b2081 |
PATRICi | fig|511145.12.peg.2159 |
Organism-specific databases
EchoBASEi | EB3813 |
Phylogenomic databases
eggNOGi | COG0826, Bacteria |
HOGENOMi | CLU_011540_0_2_6 |
InParanoidi | P76403 |
PhylomeDBi | P76403 |
Enzyme and pathway databases
BioCyci | EcoCyc:G7118-MONOMER |
Miscellaneous databases
PROi | PR:P76403 |
Family and domain databases
InterProi | View protein in InterPro IPR001539, Peptidase_U32 IPR032525, Peptidase_U32_C |
Pfami | View protein in Pfam PF01136, Peptidase_U32, 1 hit PF16325, Peptidase_U32_C, 1 hit |
PROSITEi | View protein in PROSITE PS01276, PEPTIDASE_U32, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TRHP_ECOLI | |
Accessioni | P76403Primary (citable) accession number: P76403 Secondary accession number(s): O08007, O08010 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | February 1, 1997 | |
Last modified: | April 7, 2021 | |
This is version 124 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - SIMILARITY comments
Index of protein domains and families