We will be switching to the new UniProt website soon. Please explore and share your feedback.
Take me to the new website.
UniProtKB - P76344 (ZINT_ECOLI)
Protein
Metal-binding protein ZinT
Gene
zinT
Organism
Escherichia coli (strain K12)
Status
Functioni
May function as a periplasmic zinc chaperone or mediate direct transport of zinc from the periplasm to the cytoplasm under zinc-limited conditions. Binds zinc with high affinity, and can also bind cadmium, mercury or nickel. Preferentially binds Zn2+ over Cd2+. Contains one high affinity metal binding site, and can bind additional metal ions at other sites.
2 PublicationsSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 167 | Zinc 13 Publications | 1 | |
Metal bindingi | 176 | Zinc 13 Publications | 1 | |
Metal bindingi | 176 | Zinc 23 Publications | 1 | |
Metal bindingi | 178 | Zinc 13 Publications | 1 | |
Metal bindingi | 212 | Zinc 23 Publications | 1 | |
Metal bindingi | 216 | Zinc 23 Publications | 1 |
GO - Molecular functioni
- cadmium ion binding Source: EcoCyc
- metal ion binding Source: EcoCyc
- zinc ion binding Source: EcoCyc
GO - Biological processi
- cellular response to cadmium ion Source: EcoCyc
- cellular response to hydrogen peroxide Source: EcoCyc
- cellular response to zinc ion starvation Source: EcoCyc
- cellular zinc ion homeostasis Source: EcoCyc
Keywordsi
Ligand | Cadmium, Metal-binding, Nickel, Zinc |
Enzyme and pathway databases
BioCyci | EcoCyc:G7061-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Metal-binding protein ZinTAlternative name(s): Cadmium-induced protein ZinT |
Gene namesi | Name:zinT Synonyms:yodA Ordered Locus Names:b1973, JW1956 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytosol
- cytosol Source: EcoCyc
Other locations
- outer membrane-bounded periplasmic space Source: EcoCyc
Keywords - Cellular componenti
Cytoplasm, PeriplasmPathology & Biotechi
Disruption phenotypei
Mutant shows a growth defect and a lowered cellular Zn2+ level under Zn2+ limitation. Mutant does not bind cadmium.3 Publications
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 23 | 1 PublicationAdd BLAST | 23 | |
ChainiPRO_0000013865 | 24 – 216 | Metal-binding protein ZinTAdd BLAST | 193 |
Proteomic databases
PaxDbi | P76344 |
PRIDEi | P76344 |
2D gel databases
SWISS-2DPAGEi | P76344 |
Expressioni
Inductioni
Repressed by Zur. Up-regulated in response to extreme Zn2+ deprivation. Also induced by cadmium and hydrogen peroxide.3 Publications
Interactioni
Protein-protein interaction databases
BioGRIDi | 4260387, 38 interactors 850831, 14 interactors |
IntActi | P76344, 16 interactors |
STRINGi | 511145.b1973 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P76344 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P76344 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | COG3443, Bacteria |
HOGENOMi | CLU_072001_1_0_6 |
InParanoidi | P76344 |
PhylomeDBi | P76344 |
Family and domain databases
Gene3Di | 2.40.128.20, 1 hit |
InterProi | View protein in InterPro IPR012674, Calycin IPR015304, ZinT_dom |
Pfami | View protein in Pfam PF09223, ZinT, 1 hit |
SUPFAMi | SSF50814, SSF50814, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P76344-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAIRLYKLAV ALGVFIVSAP AFSHGHHSHG KPLTEVEQKA ANGVFDDANV
60 70 80 90 100
QNRTLSDWDG VWQSVYPLLQ SGKLDPVFQK KADADKTKTF AEIKDYYHKG
110 120 130 140 150
YATDIEMIGI EDGIVEFHRN NETTSCKYDY DGYKILTYKS GKKGVRYLFE
160 170 180 190 200
CKDPESKAPK YIQFSDHIIA PRKSSHFHIF MGNDSQQSLL NEMENWPTYY
210
PYQLSSEEVV EEMMSH
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC75039.1 AP009048 Genomic DNA Translation: BAE76561.1 |
PIRi | A64962 |
RefSeqi | NP_416482.1, NC_000913.3 WP_001007805.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC75039; AAC75039; b1973 BAE76561; BAE76561; BAE76561 |
GeneIDi | 66674137 946480 |
KEGGi | ecj:JW1956 eco:b1973 |
PATRICi | fig|1411691.4.peg.277 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC75039.1 AP009048 Genomic DNA Translation: BAE76561.1 |
PIRi | A64962 |
RefSeqi | NP_416482.1, NC_000913.3 WP_001007805.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1OEE | X-ray | 2.10 | A | 24-216 | [»] | |
1OEJ | X-ray | 1.81 | A | 24-216 | [»] | |
1OEK | X-ray | 2.40 | A | 24-216 | [»] | |
1S7D | X-ray | 2.17 | A | 1-216 | [»] | |
1TXL | X-ray | 1.70 | A | 2-216 | [»] | |
5AQ6 | X-ray | 1.79 | A | 24-216 | [»] | |
5XM5 | X-ray | 1.49 | A/B | 24-216 | [»] | |
5YXC | X-ray | 1.76 | A/B | 1-216 | [»] | |
6LM2 | X-ray | 2.13 | A | 32-215 | [»] | |
SMRi | P76344 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260387, 38 interactors 850831, 14 interactors |
IntActi | P76344, 16 interactors |
STRINGi | 511145.b1973 |
2D gel databases
SWISS-2DPAGEi | P76344 |
Proteomic databases
PaxDbi | P76344 |
PRIDEi | P76344 |
Protocols and materials databases
DNASUi | 946480 |
Genome annotation databases
EnsemblBacteriai | AAC75039; AAC75039; b1973 BAE76561; BAE76561; BAE76561 |
GeneIDi | 66674137 946480 |
KEGGi | ecj:JW1956 eco:b1973 |
PATRICi | fig|1411691.4.peg.277 |
Organism-specific databases
EchoBASEi | EB3802 |
Phylogenomic databases
eggNOGi | COG3443, Bacteria |
HOGENOMi | CLU_072001_1_0_6 |
InParanoidi | P76344 |
PhylomeDBi | P76344 |
Enzyme and pathway databases
BioCyci | EcoCyc:G7061-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P76344 |
PROi | PR:P76344 |
Family and domain databases
Gene3Di | 2.40.128.20, 1 hit |
InterProi | View protein in InterPro IPR012674, Calycin IPR015304, ZinT_dom |
Pfami | View protein in Pfam PF09223, ZinT, 1 hit |
SUPFAMi | SSF50814, SSF50814, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ZINT_ECOLI | |
Accessioni | P76344Primary (citable) accession number: P76344 Secondary accession number(s): Q2MAZ5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 15, 1998 |
Last sequence update: | February 1, 1997 | |
Last modified: | February 23, 2022 | |
This is version 148 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families