UniProtKB - P76343 (MSRQ_ECOLI)
Protein
Protein-methionine-sulfoxide reductase heme-binding subunit MsrQ
Gene
msrQ
Organism
Escherichia coli (strain K12)
Status
Functioni
Part of the MsrPQ system that repairs oxidized periplasmic proteins containing methionine sulfoxide residues (Met-O), using respiratory chain electrons. Thus protects these proteins from oxidative-stress damage caused by reactive species of oxygen and chlorine. MsrPQ is essential for the maintenance of envelope integrity under bleach stress, rescuing a wide series of structurally unrelated periplasmic proteins from methionine oxidation, including the primary periplasmic chaperone SurA and the lipoprotein Pal. MsrQ provides electrons for reduction to the reductase catalytic subunit MsrP, using the quinone pool of the respiratory chain.1 Publication
Cofactori
Protein has several cofactor binding sites:- FMN1 PublicationNote: Binds 1 FMN per subunit.1 Publication
- heme b1 PublicationNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.1 Publication
Redox potential
E0 is -8 +/-16 mV at pH 7 for heme b.1 Publication
GO - Molecular functioni
- electron transfer activity Source: UniProtKB-UniRule
- FMN binding Source: EcoCyc
- heme binding Source: EcoCyc
- metal ion binding Source: UniProtKB-KW
- oxidoreductase activity, acting on diphenols and related substances as donors Source: EcoCyc
GO - Biological processi
- protein repair Source: UniProtKB
Keywordsi
Biological process | Electron transport, Transport |
Ligand | Flavoprotein, FMN, Heme, Iron, Metal-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:G7060-MONOMER MetaCyc:G7060-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Protein-methionine-sulfoxide reductase heme-binding subunit MsrQUniRule annotation1 PublicationAlternative name(s): Flavocytochrome MsrQUniRule annotation |
Gene namesi | Name:msrQ1 Publication Synonyms:yedZImported Ordered Locus Names:b1972, JW1955 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane ; Multi-pass membrane protein UniRule annotation1 Publication
Plasma Membrane
- integral component of plasma membrane Source: EcoliWiki
- plasma membrane Source: EcoCyc
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 9 | CytoplasmicCurated | 9 | |
Transmembranei | 10 – 30 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 31 – 44 | PeriplasmicCuratedAdd BLAST | 14 | |
Transmembranei | 45 – 67 | HelicalUniRule annotationAdd BLAST | 23 | |
Topological domaini | 68 – 81 | CytoplasmicCuratedAdd BLAST | 14 | |
Transmembranei | 82 – 102 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 103 – 115 | PeriplasmicCuratedAdd BLAST | 13 | |
Transmembranei | 116 – 136 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 137 – 149 | CytoplasmicCuratedAdd BLAST | 13 | |
Transmembranei | 150 – 167 | HelicalUniRule annotationAdd BLAST | 18 | |
Topological domaini | 168 – 170 | PeriplasmicCurated | 3 | |
Transmembranei | 171 – 189 | HelicalUniRule annotationAdd BLAST | 19 | |
Topological domaini | 190 – 211 | CytoplasmicCuratedAdd BLAST | 22 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Cells lacking the msrPQ genes display no visible growth defect.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000091574 | 1 – 211 | Protein-methionine-sulfoxide reductase heme-binding subunit MsrQAdd BLAST | 211 |
Proteomic databases
PaxDbi | P76343 |
PRIDEi | P76343 |
Interactioni
Subunit structurei
Heterodimer of a catalytic subunit (MsrP) and a heme-binding subunit (MsrQ).
1 PublicationProtein-protein interaction databases
BioGRIDi | 4260772, 11 interactors |
IntActi | P76343, 1 interactor |
STRINGi | 511145.b1972 |
Family & Domainsi
Sequence similaritiesi
Belongs to the MsrQ family.UniRule annotation
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG2717, Bacteria |
HOGENOMi | CLU_080662_1_0_6 |
InParanoidi | P76343 |
PhylomeDBi | P76343 |
Family and domain databases
HAMAPi | MF_01207, MsrQ, 1 hit |
InterProi | View protein in InterPro IPR013130, Fe3_Rdtase_TM_dom IPR022837, MsrQ-like |
PANTHERi | PTHR36964, PTHR36964, 1 hit |
Pfami | View protein in Pfam PF01794, Ferric_reduct, 1 hit |
i Sequence
Sequence statusi: Complete.
P76343-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRLTAKQVTW LKVCLHLAGL LPFLWLVWAI NHGGLGADPV KDIQHFTGRT
60 70 80 90 100
ALKFLLATLL ITPLARYAKQ PLLIRTRRLL GLWCFAWATL HLTSYALLEL
110 120 130 140 150
GVNNLALLGK ELITRPYLTL GIISWVILLA LAFTSTQAMQ RKLGKHWQQL
160 170 180 190 200
HNFVYLVAIL APIHYLWSVK IISPQPLIYA GLAVLLLALR YKKLRSLFNR
210
LRKQVHNKLS V
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC75038.1 AP009048 Genomic DNA Translation: BAE76560.1 |
PIRi | H64961 |
RefSeqi | NP_416481.1, NC_000913.3 WP_001240091.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC75038; AAC75038; b1972 BAE76560; BAE76560; BAE76560 |
GeneIDi | 58462953 946483 |
KEGGi | ecj:JW1955 eco:b1972 |
PATRICi | fig|1411691.4.peg.278 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC75038.1 AP009048 Genomic DNA Translation: BAE76560.1 |
PIRi | H64961 |
RefSeqi | NP_416481.1, NC_000913.3 WP_001240091.1, NZ_LN832404.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 4260772, 11 interactors |
IntActi | P76343, 1 interactor |
STRINGi | 511145.b1972 |
Proteomic databases
PaxDbi | P76343 |
PRIDEi | P76343 |
Genome annotation databases
EnsemblBacteriai | AAC75038; AAC75038; b1972 BAE76560; BAE76560; BAE76560 |
GeneIDi | 58462953 946483 |
KEGGi | ecj:JW1955 eco:b1972 |
PATRICi | fig|1411691.4.peg.278 |
Organism-specific databases
EchoBASEi | EB3801 |
Phylogenomic databases
eggNOGi | COG2717, Bacteria |
HOGENOMi | CLU_080662_1_0_6 |
InParanoidi | P76343 |
PhylomeDBi | P76343 |
Enzyme and pathway databases
BioCyci | EcoCyc:G7060-MONOMER MetaCyc:G7060-MONOMER |
Miscellaneous databases
PROi | PR:P76343 |
Family and domain databases
HAMAPi | MF_01207, MsrQ, 1 hit |
InterProi | View protein in InterPro IPR013130, Fe3_Rdtase_TM_dom IPR022837, MsrQ-like |
PANTHERi | PTHR36964, PTHR36964, 1 hit |
Pfami | View protein in Pfam PF01794, Ferric_reduct, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MSRQ_ECOLI | |
Accessioni | P76343Primary (citable) accession number: P76343 Secondary accession number(s): Q2MAZ6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 16, 2002 |
Last sequence update: | February 1, 1997 | |
Last modified: | April 7, 2021 | |
This is version 142 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Uncharacterized protein families (UPF)
List of uncharacterized protein family (UPF) entries - SIMILARITY comments
Index of protein domains and families