UniProtKB - P76342 (MSRP_ECOLI)
Protein
Protein-methionine-sulfoxide reductase catalytic subunit MsrP
Gene
msrP
Organism
Escherichia coli (strain K12)
Status
Functioni
Part of the MsrPQ system that repairs oxidized periplasmic proteins containing methionine sulfoxide residues (Met-O), using respiratory chain electrons (PubMed:26641313). Thus protects these proteins from oxidative-stress damage caused by reactive species of oxygen and chlorine (PubMed:26641313). MsrPQ is essential for the maintenance of envelope integrity under bleach stress, rescuing a wide series of structurally unrelated periplasmic proteins from methionine oxidation, including the primary periplasmic chaperone SurA and the lipoprotein Pal (PubMed:26641313). The catalytic subunit MsrP is non-stereospecific, being able to reduce both (R-) and (S-) diastereoisomers of methionine sulfoxide (PubMed:26641313). Can catalyze the reduction of a variety of substrates in vitro, including dimethyl sulfoxide, trimethylamine N-oxide, phenylmethyl sulfoxide and L-methionine sulfoxide (PubMed:15355966). Cannot reduce cyclic N-oxides (PubMed:15355966). Shows no activity as sulfite oxidase (PubMed:15355966).2 Publications
Catalytic activityi
Cofactori
Mo-molybdopterin1 PublicationNote: Binds 1 Mo-molybdopterin (Mo-MPT) cofactor per subunit. The oxidation state of Mo is +5. Is inactive in the presence of the tungsten-substituted form (W-MPT) of the cofactor.1 Publication
Kineticsi
kcat is 30.5 sec(-1) with N-acetyl-Met-O as substrate. kcat is 36.0 sec(-1) with L-methionine (S)-S-oxide as substrate. kcat is 168.3 sec(-1) with L-methionine (R)-S-oxide as substrate.1 Publication
- KM=12 mM for dimethyl sulfoxide1 Publication
- KM=22 mM for trimethylamine N-oxide1 Publication
- KM=27.9 mM for tetramethylene sulfoxide1 Publication
- KM=119 mM for L-methionine sulfoxide1 Publication
- KM=3.8 mM for N-acetyl-Met-O1 Publication
- KM=8.0 mM for L-methionine (S)-S-oxide1 Publication
- KM=25.7 mM for L-methionine (R)-S-oxide1 Publication
- Vmax=56.3 µmol/min/mg enzyme with N-acetyl-Met-O as substrate1 Publication
- Vmax=67.2 µmol/min/mg enzyme with L-methionine (S)-S-oxide as substrate1 Publication
- Vmax=313.4 µmol/min/mg enzyme with L-methionine (R)-S-oxide as substrate1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 88 | Molybdopterin1 Publication | 1 | |
Metal bindingi | 146 | Molybdenum1 Publication | 1 | |
Binding sitei | 181 | Molybdopterin1 Publication | 1 | |
Binding sitei | 233 | Molybdopterin1 Publication | 1 | |
Binding sitei | 238 | Molybdopterin1 Publication | 1 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- molybdopterin cofactor binding Source: EcoCyc
- oxidoreductase activity, acting on a heme group of donors Source: EcoCyc
- oxidoreductase activity, acting on a sulfur group of donors, quinone or similar compound as acceptor Source: UniProtKB
GO - Biological processi
- oxidation-reduction process Source: UniProtKB
- protein repair Source: UniProtKB
- response to hypochlorite Source: UniProtKB
- response to oxidative stress Source: EcoCyc
Keywordsi
Molecular function | Oxidoreductase |
Ligand | Metal-binding, Molybdenum |
Enzyme and pathway databases
BioCyci | EcoCyc:G7059-MONOMER MetaCyc:G7059-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Protein-methionine-sulfoxide reductase catalytic subunit MsrPUniRule annotation1 Publication (EC:1.8.5.-UniRule annotation1 Publication) |
Gene namesi | Name:msrP1 Publication Synonyms:yedYImported Ordered Locus Names:b1971, JW1954 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Periplasm 1 Publication Note: Is attached to the inner membrane when interacting with the MsrQ subunit.
GO - Cellular componenti
- outer membrane-bounded periplasmic space Source: EcoCyc
Keywords - Cellular componenti
PeriplasmPathology & Biotechi
Disruption phenotypei
Cells lacking the msrPQ genes display no visible growth defect.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 146 | C → S: Loss of enzymatic activity. Enhances binding to MsrQ and targeting to the inner membrane. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 44 | Tat-type signal1 PublicationAdd BLAST | 44 | |
ChainiPRO_0000070684 | 45 – 334 | Protein-methionine-sulfoxide reductase catalytic subunit MsrPAdd BLAST | 290 |
Post-translational modificationi
Exported by the Tat system. Can also be exported by the Sec system.1 Publication
Proteomic databases
jPOSTi | P76342 |
PaxDbi | P76342 |
PRIDEi | P76342 |
Expressioni
Inductioni
Is induced at protein level by hypochlorous acid (HOCl), a powerful antimicrobial released by neutrophils, but not by H2O2. Induction by HOCl is dependent on the presence of a functional YedV/YedW two-component system.1 Publication
Interactioni
Subunit structurei
Heterodimer of a catalytic subunit (MsrP) and a heme-binding subunit (MsrQ).
1 PublicationProtein-protein interaction databases
BioGRIDi | 4259675, 54 interactors |
DIPi | DIP-47888N |
IntActi | P76342, 2 interactors |
STRINGi | 511145.b1971 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P76342 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P76342 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 91 – 92 | Molybdopterin binding1 Publication | 2 | |
Regioni | 249 – 251 | Molybdopterin binding1 Publication | 3 |
Sequence similaritiesi
Belongs to the MsrP family.UniRule annotation
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | COG2041, Bacteria |
HOGENOMi | CLU_045520_0_0_6 |
InParanoidi | P76342 |
PhylomeDBi | P76342 |
Family and domain databases
Gene3Di | 3.90.420.10, 1 hit |
HAMAPi | MF_01206, MsrP, 1 hit |
InterProi | View protein in InterPro IPR022867, MsrP IPR000572, OxRdtase_Mopterin-bd_dom IPR036374, OxRdtase_Mopterin-bd_sf IPR006311, TAT_signal |
Pfami | View protein in Pfam PF00174, Oxidored_molyb, 1 hit |
SUPFAMi | SSF56524, SSF56524, 1 hit |
PROSITEi | View protein in PROSITE PS51318, TAT, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P76342-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKKNQFLKES DVTAESVFFM KRRQVLKALG ISATALSLPH AAHADLLSWF
60 70 80 90 100
KGNDRPPAPA GKALEFSKPA AWQNNLPLTP ADKVSGYNNF YEFGLDKADP
110 120 130 140 150
AANAGSLKTD PWTLKISGEV AKPLTLDHDD LTRRFPLEER IYRMRCVEAW
160 170 180 190 200
SMVVPWIGFP LHKLLALAEP TSNAKYVAFE TIYAPEQMPG QQDRFIGGGL
210 220 230 240 250
KYPYVEGLRL DEAMHPLTLM TVGVYGKALP PQNGAPVRLI VPWKYGFKGI
260 270 280 290 300
KSIVSIKLTR ERPPTTWNLA APDEYGFYAN VNPYVDHPRW SQATERFIGS
310 320 330
GGILDVQRQP TLLFNGYAAQ VASLYRGLDL RENF
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC75037.1 AP009048 Genomic DNA Translation: BAE76559.1 |
PIRi | G64961 |
RefSeqi | NP_416480.1, NC_000913.3 |
Genome annotation databases
EnsemblBacteriai | AAC75037; AAC75037; b1971 BAE76559; BAE76559; BAE76559 |
GeneIDi | 946484 |
KEGGi | ecj:JW1954 eco:b1971 |
PATRICi | fig|511145.12.peg.2051 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC75037.1 AP009048 Genomic DNA Translation: BAE76559.1 |
PIRi | G64961 |
RefSeqi | NP_416480.1, NC_000913.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1XDQ | X-ray | 2.55 | A/B/C/D/E | 45-334 | [»] | |
1XDY | X-ray | 2.20 | A/B/C/D/E/F/G/H/I/J | 45-334 | [»] | |
SMRi | P76342 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259675, 54 interactors |
DIPi | DIP-47888N |
IntActi | P76342, 2 interactors |
STRINGi | 511145.b1971 |
Proteomic databases
jPOSTi | P76342 |
PaxDbi | P76342 |
PRIDEi | P76342 |
Genome annotation databases
EnsemblBacteriai | AAC75037; AAC75037; b1971 BAE76559; BAE76559; BAE76559 |
GeneIDi | 946484 |
KEGGi | ecj:JW1954 eco:b1971 |
PATRICi | fig|511145.12.peg.2051 |
Organism-specific databases
EchoBASEi | EB3800 |
Phylogenomic databases
eggNOGi | COG2041, Bacteria |
HOGENOMi | CLU_045520_0_0_6 |
InParanoidi | P76342 |
PhylomeDBi | P76342 |
Enzyme and pathway databases
BioCyci | EcoCyc:G7059-MONOMER MetaCyc:G7059-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P76342 |
PROi | PR:P76342 |
Family and domain databases
Gene3Di | 3.90.420.10, 1 hit |
HAMAPi | MF_01206, MsrP, 1 hit |
InterProi | View protein in InterPro IPR022867, MsrP IPR000572, OxRdtase_Mopterin-bd_dom IPR036374, OxRdtase_Mopterin-bd_sf IPR006311, TAT_signal |
Pfami | View protein in Pfam PF00174, Oxidored_molyb, 1 hit |
SUPFAMi | SSF56524, SSF56524, 1 hit |
PROSITEi | View protein in PROSITE PS51318, TAT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MSRP_ECOLI | |
Accessioni | P76342Primary (citable) accession number: P76342 Secondary accession number(s): Q2MAZ7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 16, 2002 |
Last sequence update: | February 1, 1997 | |
Last modified: | December 2, 2020 | |
This is version 147 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Uncharacterized protein families (UPF)
List of uncharacterized protein family (UPF) entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families