UniProtKB - P76339 (HPRS_ECOLI)
Protein
Sensor histidine kinase HprS
Gene
hprS
Organism
Escherichia coli (strain K12)
Status
Functioni
Member of a two-component regulatory system HprR/HprS involved in response to hydrogen peroxide (PubMed:25568260, PubMed:27983483). Senses H2O2, maybe via the redox state of the membrane (PubMed:27983483). Activates HprR by phosphorylation (PubMed:15522865). Can also phosphorylate CusR (PubMed:15522865).3 Publications
Miscellaneous
HprSR and CusSR form a unique regulation system, where both two-component systems recognize and regulate the same set of genes, but under different environmental conditions. HprSR plays a role in H2O2 response regulation, while CusSR plays a role in Cu2+ response regulation.2 Publications
Catalytic activityi
- ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.1 Publication EC:2.7.13.3
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 165 | Essential for the response to H(2)O(2)1 Publication | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- phosphorelay sensor kinase activity Source: InterPro
- protein kinase activity Source: EcoCyc
GO - Biological processi
- cellular response to DNA damage stimulus Source: EcoliWiki
- phosphorelay signal transduction system Source: GO_Central
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Two-component regulatory system |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:G7056-MONOMER MetaCyc:G7056-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Sensor histidine kinase HprSCurated (EC:2.7.13.31 Publication)Alternative name(s): Hydrogen peroxide response sensor1 Publication |
Gene namesi | Name:hprS1 Publication Synonyms:yedV Ordered Locus Names:b1968, JW1951 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cell inner membrane 1 Publication; Multi-pass membrane protein Sequence analysis
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 9 | CytoplasmicCurated | 9 | |
Transmembranei | 10 – 30 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 31 – 158 | PeriplasmicCuratedAdd BLAST | 128 | |
Transmembranei | 159 – 179 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 180 – 452 | Cytoplasmic1 PublicationAdd BLAST | 273 |
GO - Cellular componenti
- integral component of membrane Source: UniProtKB-KW
- plasma membrane Source: EcoCyc
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 165 | C → A: Loss of H(2)O(2)-dependent transcription of hiuH. 1 Publication | 1 | |
Mutagenesisi | 333 | C → A: No change in activity. 1 Publication | 1 | |
Mutagenesisi | 338 | C → A: No change in activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000074914 | 1 – 452 | Sensor histidine kinase HprSAdd BLAST | 452 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 245 | Phosphohistidine; by autocatalysisPROSITE-ProRule annotation | 1 |
Post-translational modificationi
Autophosphorylated.1 Publication
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | P76339 |
PRIDEi | P76339 |
Interactioni
Binary interactionsi
P76339
With | #Exp. | IntAct |
---|---|---|
ygbT [Q46896] | 3 | EBI-554869,EBI-1130209 |
Protein-protein interaction databases
BioGRIDi | 4259161, 16 interactors 850838, 1 interactor |
DIPi | DIP-11852N |
IntActi | P76339, 4 interactors |
STRINGi | 511145.b1968 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 181 – 234 | HAMPPROSITE-ProRule annotationAdd BLAST | 54 | |
Domaini | 242 – 452 | Histidine kinasePROSITE-ProRule annotationAdd BLAST | 211 |
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG2205, Bacteria |
HOGENOMi | CLU_000445_89_6_6 |
InParanoidi | P76339 |
PhylomeDBi | P76339 |
Family and domain databases
CDDi | cd06225, HAMP, 1 hit cd00082, HisKA, 1 hit |
Gene3Di | 3.30.565.10, 1 hit |
InterProi | View protein in InterPro IPR006290, CztS_silS_copS IPR003660, HAMP_dom IPR003594, HATPase_C IPR036890, HATPase_C_sf IPR005467, His_kinase_dom IPR003661, HisK_dim/P IPR036097, HisK_dim/P_sf IPR004358, Sig_transdc_His_kin-like_C |
Pfami | View protein in Pfam PF02518, HATPase_c, 1 hit PF00512, HisKA, 1 hit |
PRINTSi | PR00344, BCTRLSENSOR |
SMARTi | View protein in SMART SM00387, HATPase_c, 1 hit SM00388, HisKA, 1 hit |
SUPFAMi | SSF47384, SSF47384, 1 hit SSF55874, SSF55874, 1 hit |
TIGRFAMsi | TIGR01386, cztS_silS_copS, 1 hit |
PROSITEi | View protein in PROSITE PS50885, HAMP, 1 hit PS50109, HIS_KIN, 1 hit |
i Sequence
Sequence statusi: Complete.
P76339-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKRLSITVRL TLLFILLLSV AGAGIVWTLY NGLASELKWR DDTTLINRTA
60 70 80 90 100
QIKQLLIDGV NPDTLPVYFN RMMDVSQDIL IIHGDSINKI VNRTNVSDGM
110 120 130 140 150
LNNIPASETI SAAGIYRSII NDTEIDALRI NIDEVSPSLT VTVAKLASAR
160 170 180 190 200
HNMLEQYKIN SIIICIVAIV LCSVLSPLLI RTGLREIKKL SGVTEALNYN
210 220 230 240 250
DSREPVEVSA LPRELKPLGQ ALNKMHHALV KDFERLSQFA DDLAHELRTP
260 270 280 290 300
INALLGQNQV TLSQTRSIAE YQKTIAGNIE ELENISRLTE NILFLARADK
310 320 330 340 350
NNVLVKLDSL SLNKEVENLL DYLEYLSDEK EICFKVECNQ QIFADKILLQ
360 370 380 390 400
RMLSNLIVNA IRYSPEKSRI HITSFLDTNS YLNIDIASPG TKINEPEKLF
410 420 430 440 450
RRFWRGDNSR HSVGQGLGLS LVKAIAELHG GSATYHYLNK HNVFRITLPQ
RN
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC75034.1 AP009048 Genomic DNA Translation: BAA15795.1 |
PIRi | D64961 |
RefSeqi | NP_416477.1, NC_000913.3 WP_000826792.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC75034; AAC75034; b1968 BAA15795; BAA15795; BAA15795 |
GeneIDi | 946487 |
KEGGi | ecj:JW1951 eco:b1968 |
PATRICi | fig|1411691.4.peg.282 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC75034.1 AP009048 Genomic DNA Translation: BAA15795.1 |
PIRi | D64961 |
RefSeqi | NP_416477.1, NC_000913.3 WP_000826792.1, NZ_LN832404.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 4259161, 16 interactors 850838, 1 interactor |
DIPi | DIP-11852N |
IntActi | P76339, 4 interactors |
STRINGi | 511145.b1968 |
Proteomic databases
PaxDbi | P76339 |
PRIDEi | P76339 |
Genome annotation databases
EnsemblBacteriai | AAC75034; AAC75034; b1968 BAA15795; BAA15795; BAA15795 |
GeneIDi | 946487 |
KEGGi | ecj:JW1951 eco:b1968 |
PATRICi | fig|1411691.4.peg.282 |
Organism-specific databases
EchoBASEi | EB3797 |
Phylogenomic databases
eggNOGi | COG2205, Bacteria |
HOGENOMi | CLU_000445_89_6_6 |
InParanoidi | P76339 |
PhylomeDBi | P76339 |
Enzyme and pathway databases
BioCyci | EcoCyc:G7056-MONOMER MetaCyc:G7056-MONOMER |
Miscellaneous databases
PROi | PR:P76339 |
Family and domain databases
CDDi | cd06225, HAMP, 1 hit cd00082, HisKA, 1 hit |
Gene3Di | 3.30.565.10, 1 hit |
InterProi | View protein in InterPro IPR006290, CztS_silS_copS IPR003660, HAMP_dom IPR003594, HATPase_C IPR036890, HATPase_C_sf IPR005467, His_kinase_dom IPR003661, HisK_dim/P IPR036097, HisK_dim/P_sf IPR004358, Sig_transdc_His_kin-like_C |
Pfami | View protein in Pfam PF02518, HATPase_c, 1 hit PF00512, HisKA, 1 hit |
PRINTSi | PR00344, BCTRLSENSOR |
SMARTi | View protein in SMART SM00387, HATPase_c, 1 hit SM00388, HisKA, 1 hit |
SUPFAMi | SSF47384, SSF47384, 1 hit SSF55874, SSF55874, 1 hit |
TIGRFAMsi | TIGR01386, cztS_silS_copS, 1 hit |
PROSITEi | View protein in PROSITE PS50885, HAMP, 1 hit PS50109, HIS_KIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HPRS_ECOLI | |
Accessioni | P76339Primary (citable) accession number: P76339 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | February 1, 1997 | |
Last modified: | December 2, 2020 | |
This is version 167 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |