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UniProtKB - P76291 (CMOB_ECOLI)
Protein
tRNA U34 carboxymethyltransferase
Gene
cmoB
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes carboxymethyl transfer from carboxy-S-adenosyl-L-methionine (Cx-SAM) to 5-hydroxyuridine (ho5U) to form 5-carboxymethoxyuridine (cmo5U) at position 34 in tRNAs. Can also catalyze the SAM-dependent methylation of ho5U, with much lower efficiency.
2 PublicationsCatalytic activityi
- 5-hydroxyuridine34 in tRNA + carboxy-S-adenosyl-L-methionine = 5-carboxymethoxyuridine34 in tRNA + H+ + S-adenosyl-L-homocysteineUniRule annotation2 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 91 | Carboxy-S-adenosyl-L-methionineUniRule annotationCombined sources | 1 | |
Binding sitei | 105 | Carboxy-S-adenosyl-L-methionineUniRule annotationCombined sources | 1 | |
Binding sitei | 110 | Carboxy-S-adenosyl-L-methionineUniRule annotationCombined sources | 1 | |
Binding sitei | 130 | Carboxy-S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotationCombined sources | 1 | |
Binding sitei | 196 | Carboxy-S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotationCombined sources | 1 | |
Binding sitei | 200 | Carboxy-S-adenosyl-L-methionineUniRule annotationCombined sources | 1 | |
Binding sitei | 315 | Carboxy-S-adenosyl-L-methionineUniRule annotationCombined sources | 1 |
GO - Molecular functioni
- identical protein binding Source: EcoCyc
- transferase activity, transferring alkyl or aryl (other than methyl) groups Source: EcoCyc
GO - Biological processi
- tRNA wobble uridine modification Source: EcoCyc
Keywordsi
Molecular function | Transferase |
Biological process | tRNA processing |
Enzyme and pathway databases
BioCyci | EcoCyc:G7021-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: tRNA U34 carboxymethyltransferaseUniRule annotationCurated (EC:2.5.1.-UniRule annotation2 Publications) |
Gene namesi | Name:cmoB1 PublicationUniRule annotation Synonyms:yecP Ordered Locus Names:b1871, JW1860 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 91 | K → A: Decreases affinity toward Cx-SAM and increases affinity toward SAM. Lack of carboxymethyltransferase activity, but can still produce mho5U. | 1 | |
Mutagenesisi | 200 | Y → A: Slight decrease in Cx-SAM binding affinity. Does not affect carboxymethyltransferase activity. | 1 | |
Mutagenesisi | 315 | R → A: Slight decrease in Cx-SAM binding affinity. Does not affect carboxymethyltransferase activity. | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000169085 | 1 – 323 | tRNA U34 carboxymethyltransferaseAdd BLAST | 323 |
Proteomic databases
jPOSTi | P76291 |
PaxDbi | P76291 |
PRIDEi | P76291 |
Interactioni
Subunit structurei
Homotetramer.
UniRule annotation1 PublicationGO - Molecular functioni
- identical protein binding Source: EcoCyc
Protein-protein interaction databases
BioGRIDi | 4260349, 43 interactors |
IntActi | P76291, 7 interactors |
STRINGi | 511145.b1871 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P76291 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 152 – 154 | Carboxy-S-adenosyl-L-methionine bindingUniRule annotationCombined sources | 3 | |
Regioni | 181 – 182 | Carboxy-S-adenosyl-L-methionine bindingUniRule annotationCombined sources | 2 |
Sequence similaritiesi
Belongs to the class I-like SAM-binding methyltransferase superfamily. CmoB family.UniRule annotationCurated
Phylogenomic databases
eggNOGi | COG0500, Bacteria |
HOGENOMi | CLU_052665_0_0_6 |
InParanoidi | P76291 |
PhylomeDBi | P76291 |
Family and domain databases
HAMAPi | MF_01590, tRNA_carboxymethyltr_CmoB, 1 hit |
InterProi | View protein in InterPro IPR010017, CmoB IPR027555, Mo5U34_MeTrfas-like IPR029063, SAM-dependent_MTases |
Pfami | View protein in Pfam PF08003, Methyltransf_9, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
TIGRFAMsi | TIGR00452, TIGR00452, 1 hit |
i Sequence
Sequence statusi: Complete.
P76291-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIDFGNFYSL IAKNHLSHWL ETLPAQIANW QREQQHGLFK QWSNAVEFLP
60 70 80 90 100
EIKPYRLDLL HSVTAESEEP LSAGQIKRIE TLMRNLMPWR KGPFSLYGVN
110 120 130 140 150
IDTEWRSDWK WDRVLPHLSD LTGRTILDVG CGSGYHMWRM IGAGAHLAVG
160 170 180 190 200
IDPTQLFLCQ FEAVRKLLGN DQRAHLLPLG IEQLPALKAF DTVFSMGVLY
210 220 230 240 250
HRRSPLEHLW QLKDQLVNEG ELVLETLVID GDENTVLVPG DRYAQMRNVY
260 270 280 290 300
FIPSALALKN WLKKCGFVDI RIADVSVTTT EEQRRTEWMV TESLADFLDP
310 320
HDPGKTVEGY PAPKRAVLIA RKP
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74941.1 AP009048 Genomic DNA Translation: BAA15681.1 |
PIRi | G64949 |
RefSeqi | NP_416385.1, NC_000913.3 WP_000564725.1, NZ_SSZK01000001.1 |
Genome annotation databases
EnsemblBacteriai | AAC74941; AAC74941; b1871 BAA15681; BAA15681; BAA15681 |
GeneIDi | 58462338 946387 |
KEGGi | ecj:JW1860 eco:b1871 |
PATRICi | fig|1411691.4.peg.377 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74941.1 AP009048 Genomic DNA Translation: BAA15681.1 |
PIRi | G64949 |
RefSeqi | NP_416385.1, NC_000913.3 WP_000564725.1, NZ_SSZK01000001.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4QNU | X-ray | 2.60 | A/B/C/D/E/F/G/H | 1-323 | [»] | |
4QNV | X-ray | 2.64 | A/B | 1-323 | [»] | |
4QNX | X-ray | 2.62 | A/B | 1-323 | [»] | |
SMRi | P76291 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260349, 43 interactors |
IntActi | P76291, 7 interactors |
STRINGi | 511145.b1871 |
Proteomic databases
jPOSTi | P76291 |
PaxDbi | P76291 |
PRIDEi | P76291 |
Genome annotation databases
EnsemblBacteriai | AAC74941; AAC74941; b1871 BAA15681; BAA15681; BAA15681 |
GeneIDi | 58462338 946387 |
KEGGi | ecj:JW1860 eco:b1871 |
PATRICi | fig|1411691.4.peg.377 |
Organism-specific databases
EchoBASEi | EB3788 |
Phylogenomic databases
eggNOGi | COG0500, Bacteria |
HOGENOMi | CLU_052665_0_0_6 |
InParanoidi | P76291 |
PhylomeDBi | P76291 |
Enzyme and pathway databases
BioCyci | EcoCyc:G7021-MONOMER |
Miscellaneous databases
PROi | PR:P76291 |
Family and domain databases
HAMAPi | MF_01590, tRNA_carboxymethyltr_CmoB, 1 hit |
InterProi | View protein in InterPro IPR010017, CmoB IPR027555, Mo5U34_MeTrfas-like IPR029063, SAM-dependent_MTases |
Pfami | View protein in Pfam PF08003, Methyltransf_9, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
TIGRFAMsi | TIGR00452, TIGR00452, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CMOB_ECOLI | |
Accessioni | P76291Primary (citable) accession number: P76291 Secondary accession number(s): O07983 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | February 1, 1997 | |
Last modified: | February 23, 2022 | |
This is version 144 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families