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Protein

Ribosomal RNA small subunit methyltransferase F

Gene

rsmF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + cytosine(1407) in 16S rRNA = S-adenosyl-L-homocysteine + 5-methylcytosine(1407) in 16S rRNA.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei149S-adenosyl-L-methionineBy similarity1
Binding sitei176S-adenosyl-L-methionineBy similarity1
Binding sitei194S-adenosyl-L-methionineBy similarity1
Active sitei247NucleophileBy similarity1

GO - Molecular functioni

  • RNA binding Source: UniProtKB-KW
  • rRNA (cytosine-C5-)-methyltransferase activity Source: EcoCyc

GO - Biological processi

  • rRNA base methylation Source: EcoCyc

Keywordsi

Molecular functionMethyltransferase, RNA-binding, Transferase
Biological processrRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:G7008-MONOMER
MetaCyc:G7008-MONOMER
BRENDAi2.1.1.178 2026

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA small subunit methyltransferase F (EC:2.1.1.178)
Alternative name(s):
16S rRNA m5C1407 methyltransferase
rRNA (cytosine-C(5)-)-methyltransferase RsmF
Gene namesi
Name:rsmF
Synonyms:yebU
Ordered Locus Names:b1835, JW5301
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14023 rsmF

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002118221 – 479Ribosomal RNA small subunit methyltransferase FAdd BLAST479

Proteomic databases

PaxDbiP76273
PRIDEiP76273

Interactioni

Protein-protein interaction databases

BioGridi4259158, 28 interactors
STRINGi316385.ECDH10B_1974

Structurei

Secondary structure

1479
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP76273
SMRiP76273
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP76273

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni125 – 131S-adenosyl-L-methionine bindingBy similarity7

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CYJ Bacteria
COG0144 LUCA
COG3270 LUCA
HOGENOMiHOG000218115
InParanoidiP76273
KOiK11392
PhylomeDBiP76273

Family and domain databases

HAMAPiMF_01579 16SrRNA_methyltr_F, 1 hit
InterProiView protein in InterPro
IPR018314 Fmu/NOL1/Nop2p_CS
IPR031341 Methyltr_RsmF_N
IPR001678 MeTrfase_RsmB/NOP2
IPR027391 Nol1_Nop2_Fmu_2
IPR011023 Nop2p
IPR023267 RCMT
IPR023545 rRNA_ssu_MeTfrase_F
IPR029063 SAM-dependent_MTases
PfamiView protein in Pfam
PF01189 Methyltr_RsmB-F, 1 hit
PF17125 Methyltr_RsmF_N, 1 hit
PF13636 Methyltranf_PUA, 1 hit
PRINTSiPR02008 RCMTFAMILY
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00446 nop2p, 1 hit
PROSITEiView protein in PROSITE
PS01153 NOL1_NOP2_SUN, 1 hit
PS51686 SAM_MT_RSMB_NOP, 1 hit

Sequencei

Sequence statusi: Complete.

P76273-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAQHTVYFPD AFLTQMREAM PSTLSFDDFL AACQRPLRRS IRVNTLKISV
60 70 80 90 100
ADFLQLTAPY GWTLTPIPWC EEGFWIERDN EDALPLGSTA EHLSGLFYIQ
110 120 130 140 150
EASSMLPVAA LFADGNAPQR VMDVAAAPGS KTTQISARMN NEGAILANEF
160 170 180 190 200
SASRVKVLHA NISRCGISNV ALTHFDGRVF GAAVPEMFDA ILLDAPCSGE
210 220 230 240 250
GVVRKDPDAL KNWSPESNQE IAATQRELID SAFHALRPGG TLVYSTCTLN
260 270 280 290 300
QEENEAVCLW LKETYPDAVE FLPLGDLFPG ANKALTEEGF LHVFPQIYDC
310 320 330 340 350
EGFFVARLRK TQAIPALPAP KYKVGNFPFS PVKDREAGQI RQAATGVGLN
360 370 380 390 400
WDENLRLWQR DKELWLFPVG IEALIGKVRF SRLGIKLAET HNKGYRWQHE
410 420 430 440 450
AVIALASPDN MNAFELTPQE AEEWYRGRDV YPQAAPVADD VLVTFQHQPI
460 470
GLAKRIGSRL KNSYPRELVR DGKLFTGNA
Length:479
Mass (Da):53,228
Last modified:December 15, 1998 - v2
Checksum:i30D2B6AD01FCFF3E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74905.2
AP009048 Genomic DNA Translation: BAA15648.2
PIRiC64945
RefSeqiNP_416349.2, NC_000913.3
WP_001352260.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74905; AAC74905; b1835
BAA15648; BAA15648; BAA15648
GeneIDi946348
KEGGiecj:JW5301
eco:b1835
PATRICifig|511145.12.peg.1913

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74905.2
AP009048 Genomic DNA Translation: BAA15648.2
PIRiC64945
RefSeqiNP_416349.2, NC_000913.3
WP_001352260.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2FRXX-ray2.90A/B/C/D1-479[»]
ProteinModelPortaliP76273
SMRiP76273
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259158, 28 interactors
STRINGi316385.ECDH10B_1974

Proteomic databases

PaxDbiP76273
PRIDEiP76273

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74905; AAC74905; b1835
BAA15648; BAA15648; BAA15648
GeneIDi946348
KEGGiecj:JW5301
eco:b1835
PATRICifig|511145.12.peg.1913

Organism-specific databases

EchoBASEiEB3777
EcoGeneiEG14023 rsmF

Phylogenomic databases

eggNOGiENOG4105CYJ Bacteria
COG0144 LUCA
COG3270 LUCA
HOGENOMiHOG000218115
InParanoidiP76273
KOiK11392
PhylomeDBiP76273

Enzyme and pathway databases

BioCyciEcoCyc:G7008-MONOMER
MetaCyc:G7008-MONOMER
BRENDAi2.1.1.178 2026

Miscellaneous databases

EvolutionaryTraceiP76273
PROiPR:P76273

Family and domain databases

HAMAPiMF_01579 16SrRNA_methyltr_F, 1 hit
InterProiView protein in InterPro
IPR018314 Fmu/NOL1/Nop2p_CS
IPR031341 Methyltr_RsmF_N
IPR001678 MeTrfase_RsmB/NOP2
IPR027391 Nol1_Nop2_Fmu_2
IPR011023 Nop2p
IPR023267 RCMT
IPR023545 rRNA_ssu_MeTfrase_F
IPR029063 SAM-dependent_MTases
PfamiView protein in Pfam
PF01189 Methyltr_RsmB-F, 1 hit
PF17125 Methyltr_RsmF_N, 1 hit
PF13636 Methyltranf_PUA, 1 hit
PRINTSiPR02008 RCMTFAMILY
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00446 nop2p, 1 hit
PROSITEiView protein in PROSITE
PS01153 NOL1_NOP2_SUN, 1 hit
PS51686 SAM_MT_RSMB_NOP, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRSMF_ECOLI
AccessioniPrimary (citable) accession number: P76273
Secondary accession number(s): O07980
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: December 15, 1998
Last modified: November 7, 2018
This is version 131 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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