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UniProtKB - P76273 (RSMF_ECOLI)
Protein
Ribosomal RNA small subunit methyltransferase F
Gene
rsmF
Organism
Escherichia coli (strain K12)
Status
Functioni
Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA.
1 PublicationCatalytic activityi
- cytidine1407 in 16S rRNA + S-adenosyl-L-methionine = 5-methylcytidine1407 in 16S rRNA + H+ + S-adenosyl-L-homocysteine2 PublicationsEC:2.1.1.1782 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 149 | S-adenosyl-L-methionineBy similarity | 1 | |
Binding sitei | 176 | S-adenosyl-L-methionineBy similarity | 1 | |
Binding sitei | 194 | S-adenosyl-L-methionineBy similarity | 1 | |
Active sitei | 247 | NucleophileBy similarity | 1 |
GO - Molecular functioni
- RNA binding Source: UniProtKB-KW
- rRNA (cytosine-C5-)-methyltransferase activity Source: EcoCyc
GO - Biological processi
- RNA methylation Source: GO_Central
- rRNA base methylation Source: EcoCyc
Keywordsi
Molecular function | Methyltransferase, RNA-binding, Transferase |
Biological process | rRNA processing |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
BioCyci | EcoCyc:G7008-MONOMER |
BRENDAi | 2.1.1.178, 2026 |
Names & Taxonomyi
Protein namesi | Recommended name: Ribosomal RNA small subunit methyltransferase F (EC:2.1.1.178)Alternative name(s): 16S rRNA m5C1407 methyltransferase rRNA (cytosine-C(5)-)-methyltransferase RsmF |
Gene namesi | Name:rsmF Synonyms:yebU Ordered Locus Names:b1835, JW5301 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm Curated
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000211822 | 1 – 479 | Ribosomal RNA small subunit methyltransferase FAdd BLAST | 479 |
Proteomic databases
PaxDbi | P76273 |
PRIDEi | P76273 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4259158, 28 interactors |
STRINGi | 511145.b1835 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P76273 |
SMRi | P76273 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P76273 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 125 – 131 | S-adenosyl-L-methionine bindingBy similarity | 7 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0144, Bacteria COG3270, Bacteria |
HOGENOMi | CLU_005316_6_2_6 |
InParanoidi | P76273 |
OMAi | PWCAEGF |
PhylomeDBi | P76273 |
Family and domain databases
Gene3Di | 3.40.50.150, 1 hit |
HAMAPi | MF_01579, 16SrRNA_methyltr_F, 1 hit |
InterProi | View protein in InterPro IPR018314, Fmu/NOL1/Nop2p_CS IPR031341, Methyltr_RsmF_N IPR001678, MeTrfase_RsmB/NOP2 IPR027391, Nol1_Nop2_Fmu_2 IPR011023, Nop2p IPR023267, RCMT IPR023545, rRNA_ssu_MeTfrase_F IPR029063, SAM-dependent_MTases |
PANTHERi | PTHR22807, PTHR22807, 1 hit |
Pfami | View protein in Pfam PF01189, Methyltr_RsmB-F, 1 hit PF17125, Methyltr_RsmF_N, 1 hit PF13636, Methyltranf_PUA, 1 hit |
PRINTSi | PR02008, RCMTFAMILY |
SUPFAMi | SSF53335, SSF53335, 1 hit |
TIGRFAMsi | TIGR00446, nop2p, 1 hit |
PROSITEi | View protein in PROSITE PS01153, NOL1_NOP2_SUN, 1 hit PS51686, SAM_MT_RSMB_NOP, 1 hit |
i Sequence
Sequence statusi: Complete.
P76273-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAQHTVYFPD AFLTQMREAM PSTLSFDDFL AACQRPLRRS IRVNTLKISV
60 70 80 90 100
ADFLQLTAPY GWTLTPIPWC EEGFWIERDN EDALPLGSTA EHLSGLFYIQ
110 120 130 140 150
EASSMLPVAA LFADGNAPQR VMDVAAAPGS KTTQISARMN NEGAILANEF
160 170 180 190 200
SASRVKVLHA NISRCGISNV ALTHFDGRVF GAAVPEMFDA ILLDAPCSGE
210 220 230 240 250
GVVRKDPDAL KNWSPESNQE IAATQRELID SAFHALRPGG TLVYSTCTLN
260 270 280 290 300
QEENEAVCLW LKETYPDAVE FLPLGDLFPG ANKALTEEGF LHVFPQIYDC
310 320 330 340 350
EGFFVARLRK TQAIPALPAP KYKVGNFPFS PVKDREAGQI RQAATGVGLN
360 370 380 390 400
WDENLRLWQR DKELWLFPVG IEALIGKVRF SRLGIKLAET HNKGYRWQHE
410 420 430 440 450
AVIALASPDN MNAFELTPQE AEEWYRGRDV YPQAAPVADD VLVTFQHQPI
460 470
GLAKRIGSRL KNSYPRELVR DGKLFTGNA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74905.2 AP009048 Genomic DNA Translation: BAA15648.2 |
PIRi | C64945 |
RefSeqi | NP_416349.2, NC_000913.3 WP_001352260.1, NZ_SSZK01000001.1 |
Genome annotation databases
EnsemblBacteriai | AAC74905; AAC74905; b1835 BAA15648; BAA15648; BAA15648 |
GeneIDi | 946348 |
KEGGi | ecj:JW5301 eco:b1835 |
PATRICi | fig|511145.12.peg.1913 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74905.2 AP009048 Genomic DNA Translation: BAA15648.2 |
PIRi | C64945 |
RefSeqi | NP_416349.2, NC_000913.3 WP_001352260.1, NZ_SSZK01000001.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2FRX | X-ray | 2.90 | A/B/C/D | 1-479 | [»] | |
AlphaFoldDBi | P76273 | |||||
SMRi | P76273 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259158, 28 interactors |
STRINGi | 511145.b1835 |
Proteomic databases
PaxDbi | P76273 |
PRIDEi | P76273 |
Genome annotation databases
EnsemblBacteriai | AAC74905; AAC74905; b1835 BAA15648; BAA15648; BAA15648 |
GeneIDi | 946348 |
KEGGi | ecj:JW5301 eco:b1835 |
PATRICi | fig|511145.12.peg.1913 |
Organism-specific databases
EchoBASEi | EB3777 |
Phylogenomic databases
eggNOGi | COG0144, Bacteria COG3270, Bacteria |
HOGENOMi | CLU_005316_6_2_6 |
InParanoidi | P76273 |
OMAi | PWCAEGF |
PhylomeDBi | P76273 |
Enzyme and pathway databases
BioCyci | EcoCyc:G7008-MONOMER |
BRENDAi | 2.1.1.178, 2026 |
Miscellaneous databases
EvolutionaryTracei | P76273 |
PROi | PR:P76273 |
Family and domain databases
Gene3Di | 3.40.50.150, 1 hit |
HAMAPi | MF_01579, 16SrRNA_methyltr_F, 1 hit |
InterProi | View protein in InterPro IPR018314, Fmu/NOL1/Nop2p_CS IPR031341, Methyltr_RsmF_N IPR001678, MeTrfase_RsmB/NOP2 IPR027391, Nol1_Nop2_Fmu_2 IPR011023, Nop2p IPR023267, RCMT IPR023545, rRNA_ssu_MeTfrase_F IPR029063, SAM-dependent_MTases |
PANTHERi | PTHR22807, PTHR22807, 1 hit |
Pfami | View protein in Pfam PF01189, Methyltr_RsmB-F, 1 hit PF17125, Methyltr_RsmF_N, 1 hit PF13636, Methyltranf_PUA, 1 hit |
PRINTSi | PR02008, RCMTFAMILY |
SUPFAMi | SSF53335, SSF53335, 1 hit |
TIGRFAMsi | TIGR00446, nop2p, 1 hit |
PROSITEi | View protein in PROSITE PS01153, NOL1_NOP2_SUN, 1 hit PS51686, SAM_MT_RSMB_NOP, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RSMF_ECOLI | |
Accessioni | P76273Primary (citable) accession number: P76273 Secondary accession number(s): O07980 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | December 15, 1998 | |
Last modified: | May 25, 2022 | |
This is version 147 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families