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Protein

Probable cyclic di-GMP phosphodiesterase PdeD

Gene

pdeD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Phosphodiesterase (PDE) that catalyzes the hydrolysis of cyclic-di-GMP (c-di-GMP) to 5'-pGpG (By similarity). May serve as a negative regulator of cellulose synthesis (as has been suggested for S.typhimurium); overexpression inhibits cell aggregation in strains able to produce adhesive curli fimbriae. Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria (PubMed:16513732).By similarity1 Publication

Catalytic activityi

Cyclic di-3',5'-guanylate + H2O = 5'-phosphoguanylyl(3'->5')guanosine.By similarity

GO - Molecular functioni

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoliWiki

Keywordsi

Molecular functionHydrolase
Ligandc-di-GMP

Enzyme and pathway databases

BioCyciEcoCyc:G6996-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Probable cyclic di-GMP phosphodiesterase PdeDCurated (EC:3.1.4.52By similarity)
Gene namesi
Name:pdeD1 Publication
Synonyms:adrB, yoaD
Ordered Locus Names:b1815, JW1804
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13516 adrB

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei16 – 36HelicalSequence analysisAdd BLAST21
Transmembranei245 – 265HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene show increased aggregation, increased cellulose production but no increase in surface attachment.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001690331 – 532Probable cyclic di-GMP phosphodiesterase PdeDAdd BLAST532

Proteomic databases

PaxDbiP76261
PRIDEiP76261

Expressioni

Inductioni

Expressed at 28 degrees Celsius in late stationary phase, constitutively expressed at low levels at 37 degrees Celsius, more highly expressed on plates than in liquid medium. Expression is RpoS- and CsgD-dependent.2 Publications

Interactioni

Protein-protein interaction databases

BioGridi4260346, 6 interactors
IntActiP76261, 1 interactor
STRINGi316385.ECDH10B_1953

Structurei

3D structure databases

ProteinModelPortaliP76261
SMRiP76261
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini266 – 515EALPROSITE-ProRule annotationAdd BLAST250

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4107XTP Bacteria
COG2200 LUCA
HOGENOMiHOG000273106
InParanoidiP76261
KOiK21090
OMAiIRFISQR
PhylomeDBiP76261

Family and domain databases

CDDicd01948 EAL, 1 hit
Gene3Di3.20.20.450, 1 hit
InterProiView protein in InterPro
IPR024744 CSS-motif_dom
IPR001633 EAL_dom
IPR035919 EAL_sf
PfamiView protein in Pfam
PF12792 CSS-motif, 1 hit
PF00563 EAL, 1 hit
SMARTiView protein in SMART
SM00052 EAL, 1 hit
SUPFAMiSSF141868 SSF141868, 1 hit
PROSITEiView protein in PROSITE
PS50883 EAL, 1 hit

Sequencei

Sequence statusi: Complete.

P76261-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQKAQRIIKT YRRNRMIVCT ICALVTLAST LSVRFISQRN LNQQRVVQFA
60 70 80 90 100
NHAVEELDKV LLPLQAGSEV LLPLIGLPCS VAHLPLRKQA AKLQTVRSIG
110 120 130 140 150
LVQDGTLYCS SIFGYRNVPV VDILAELPAP QPLLRLTIDR ALIKGSPVLI
160 170 180 190 200
QWTPAAGSSN AGVMEMINID LLTAMLLEPQ LPQISSASLT VDKRHLLYGN
210 220 230 240 250
GLVDSLPQPE DNENYQVSSQ RFPFTINVNG PGATALAWHY LPTQLPLAVL
260 270 280 290 300
LSLLVGYIAW LATAYRMSFS REINLGLAQH EFELFCQPLL NARSQQCIGV
310 320 330 340 350
EILLRWNNPR QGWISPDVFI PIAEEHHLIV PLTRYVMAET IRQRHVFPMS
360 370 380 390 400
SQFHVGINVA PSHFRRGVLI KDLNQYWFSA HPIQQLILEI TERDALLDVD
410 420 430 440 450
YRIARELHRK NVKLAIDDFG TGNSSFSWLE TLRPDVLKID KSFTAAIGSD
460 470 480 490 500
AVNSTVTDII IALGQRLNIE LVAEGVETQE QAKYLRRHGV HILQGYLYAQ
510 520 530
PMPLRDFPKW LAGSQPPPAR HNGHITPIMP LR
Length:532
Mass (Da):59,711
Last modified:April 27, 2001 - v2
Checksum:i977155A7D56C18C7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74885.2
AP009048 Genomic DNA Translation: BAA15622.1
PIRiG64942
RefSeqiNP_416329.4, NC_000913.3
WP_001295494.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74885; AAC74885; b1815
BAA15622; BAA15622; BAA15622
GeneIDi946336
KEGGiecj:JW1804
eco:b1815
PATRICifig|511145.12.peg.1892

Similar proteinsi

Entry informationi

Entry nameiPDED_ECOLI
AccessioniPrimary (citable) accession number: P76261
Secondary accession number(s): P97188, P97189
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: April 27, 2001
Last modified: April 25, 2018
This is version 114 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene

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