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UniProtKB - P76261 (PDED_ECOLI)
Protein
Probable cyclic di-GMP phosphodiesterase PdeD
Gene
pdeD
Organism
Escherichia coli (strain K12)
Status
Functioni
Phosphodiesterase (PDE) that catalyzes the hydrolysis of cyclic-di-GMP (c-di-GMP) to 5'-pGpG (By similarity).
May serve as a negative regulator of cellulose synthesis (as has been suggested for S.typhimurium); overexpression inhibits cell aggregation in strains able to produce adhesive curli fimbriae. Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria (PubMed:16513732).
By similarity1 PublicationCatalytic activityi
- EC:3.1.4.52By similarity
GO - Molecular functioni
- cyclic-guanylate-specific phosphodiesterase activity Source: UniProtKB-EC
GO - Biological processi
- cellular response to DNA damage stimulus Source: EcoliWiki
Keywordsi
Molecular function | Hydrolase |
Ligand | c-di-GMP |
Enzyme and pathway databases
BioCyci | EcoCyc:G6996-MONOMER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:pdeD1 Publication Synonyms:adrB, yoaD Ordered Locus Names:b1815, JW1804 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell membrane Curated; Multi-pass membrane protein Sequence analysis
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 16 – 36 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 245 – 265 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Cells lacking this gene show increased aggregation, increased cellulose production but no increase in surface attachment.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000169033 | 1 – 532 | Probable cyclic di-GMP phosphodiesterase PdeDAdd BLAST | 532 |
Proteomic databases
PaxDbi | P76261 |
PRIDEi | P76261 |
Expressioni
Inductioni
Expressed at 28 degrees Celsius in late stationary phase, constitutively expressed at low levels at 37 degrees Celsius, more highly expressed on plates than in liquid medium. Expression is RpoS- and CsgD-dependent.2 Publications
Interactioni
Protein-protein interaction databases
BioGRIDi | 4260346, 6 interactors |
IntActi | P76261, 1 interactor |
STRINGi | 511145.b1815 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 266 – 515 | EALPROSITE-ProRule annotationAdd BLAST | 250 |
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG2200, Bacteria |
HOGENOMi | CLU_000445_131_2_6 |
InParanoidi | P76261 |
PhylomeDBi | P76261 |
Family and domain databases
CDDi | cd01948, EAL, 1 hit |
Gene3Di | 3.20.20.450, 1 hit |
InterProi | View protein in InterPro IPR024744, CSS-motif_dom IPR001633, EAL_dom IPR035919, EAL_sf |
Pfami | View protein in Pfam PF12792, CSS-motif, 1 hit PF00563, EAL, 1 hit |
SMARTi | View protein in SMART SM00052, EAL, 1 hit |
SUPFAMi | SSF141868, SSF141868, 1 hit |
PROSITEi | View protein in PROSITE PS50883, EAL, 1 hit |
i Sequence
Sequence statusi: Complete.
P76261-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQKAQRIIKT YRRNRMIVCT ICALVTLAST LSVRFISQRN LNQQRVVQFA
60 70 80 90 100
NHAVEELDKV LLPLQAGSEV LLPLIGLPCS VAHLPLRKQA AKLQTVRSIG
110 120 130 140 150
LVQDGTLYCS SIFGYRNVPV VDILAELPAP QPLLRLTIDR ALIKGSPVLI
160 170 180 190 200
QWTPAAGSSN AGVMEMINID LLTAMLLEPQ LPQISSASLT VDKRHLLYGN
210 220 230 240 250
GLVDSLPQPE DNENYQVSSQ RFPFTINVNG PGATALAWHY LPTQLPLAVL
260 270 280 290 300
LSLLVGYIAW LATAYRMSFS REINLGLAQH EFELFCQPLL NARSQQCIGV
310 320 330 340 350
EILLRWNNPR QGWISPDVFI PIAEEHHLIV PLTRYVMAET IRQRHVFPMS
360 370 380 390 400
SQFHVGINVA PSHFRRGVLI KDLNQYWFSA HPIQQLILEI TERDALLDVD
410 420 430 440 450
YRIARELHRK NVKLAIDDFG TGNSSFSWLE TLRPDVLKID KSFTAAIGSD
460 470 480 490 500
AVNSTVTDII IALGQRLNIE LVAEGVETQE QAKYLRRHGV HILQGYLYAQ
510 520 530
PMPLRDFPKW LAGSQPPPAR HNGHITPIMP LR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74885.2 AP009048 Genomic DNA Translation: BAA15622.1 |
PIRi | G64942 |
RefSeqi | NP_416329.4, NC_000913.3 WP_001295494.1, NZ_SSUW01000012.1 |
Genome annotation databases
EnsemblBacteriai | AAC74885; AAC74885; b1815 BAA15622; BAA15622; BAA15622 |
GeneIDi | 66674296 946336 |
KEGGi | ecj:JW1804 eco:b1815 |
PATRICi | fig|511145.12.peg.1892 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74885.2 AP009048 Genomic DNA Translation: BAA15622.1 |
PIRi | G64942 |
RefSeqi | NP_416329.4, NC_000913.3 WP_001295494.1, NZ_SSUW01000012.1 |
3D structure databases
SMRi | P76261 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4260346, 6 interactors |
IntActi | P76261, 1 interactor |
STRINGi | 511145.b1815 |
Proteomic databases
PaxDbi | P76261 |
PRIDEi | P76261 |
Genome annotation databases
EnsemblBacteriai | AAC74885; AAC74885; b1815 BAA15622; BAA15622; BAA15622 |
GeneIDi | 66674296 946336 |
KEGGi | ecj:JW1804 eco:b1815 |
PATRICi | fig|511145.12.peg.1892 |
Organism-specific databases
EchoBASEi | EB3289 |
Phylogenomic databases
eggNOGi | COG2200, Bacteria |
HOGENOMi | CLU_000445_131_2_6 |
InParanoidi | P76261 |
PhylomeDBi | P76261 |
Enzyme and pathway databases
BioCyci | EcoCyc:G6996-MONOMER |
Miscellaneous databases
PROi | PR:P76261 |
Family and domain databases
CDDi | cd01948, EAL, 1 hit |
Gene3Di | 3.20.20.450, 1 hit |
InterProi | View protein in InterPro IPR024744, CSS-motif_dom IPR001633, EAL_dom IPR035919, EAL_sf |
Pfami | View protein in Pfam PF12792, CSS-motif, 1 hit PF00563, EAL, 1 hit |
SMARTi | View protein in SMART SM00052, EAL, 1 hit |
SUPFAMi | SSF141868, SSF141868, 1 hit |
PROSITEi | View protein in PROSITE PS50883, EAL, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PDED_ECOLI | |
Accessioni | P76261Primary (citable) accession number: P76261 Secondary accession number(s): P97188, P97189 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 27, 2001 |
Last sequence update: | April 27, 2001 | |
Last modified: | February 23, 2022 | |
This is version 130 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |