UniProtKB - P76256 (TSAB_ECOLI)
tRNA threonylcarbamoyladenosine biosynthesis protein TsaB
tsaB
Functioni
Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaD and TsaE. TsaB seems to play an indirect role in the t6A biosynthesis pathway, possibly in regulating the core enzymatic function of TsaD. In fact, can act as a protease that specifically degrades TsaD in vitro; therefore TsaB may post-translationally regulate cellular pools of TsaD via proteolytic degradation. Does not show sialoglycoprotease activity against glycophorin A.
2 PublicationsMiscellaneous
Caution
GO - Molecular functioni
- identical protein binding Source: EcoCyc
- metallopeptidase activity Source: EcoCyc
GO - Biological processi
- tRNA threonylcarbamoyladenosine modification Source: ComplexPortal
Keywordsi
Biological process | tRNA processing |
Enzyme and pathway databases
BioCyci | EcoCyc:G6991-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: tRNA threonylcarbamoyladenosine biosynthesis protein TsaBAlternative name(s): t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaB |
Gene namesi | Name:tsaB Synonyms:yeaZ Ordered Locus Names:b1807, JW1796 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Cytosol
- cytosol Source: EcoCyc
Other locations
- EKC/KEOPS complex Source: ComplexPortal
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000096990 | 1 – 231 | tRNA threonylcarbamoyladenosine biosynthesis protein TsaBAdd BLAST | 231 |
Proteomic databases
jPOSTi | P76256 |
PaxDbi | P76256 |
PRIDEi | P76256 |
Interactioni
Subunit structurei
Homodimer.
Interacts with TsaD and TsaE in a mutually exclusive manner; TsaD is the preferred partner.
Also interacts with TsaC.
3 PublicationsBinary interactionsi
P76256
With | #Exp. | IntAct |
---|---|---|
tsaD [P05852] | 8 | EBI-560669,EBI-561994 |
GO - Molecular functioni
- identical protein binding Source: EcoCyc
Protein-protein interaction databases
BioGRIDi | 4260340, 15 interactors 850661, 5 interactors |
ComplexPortali | CPX-1094, YgjD-YeaZ-YjeE complex |
DIPi | DIP-11804N |
IntActi | P76256, 12 interactors |
STRINGi | 511145.b1807 |
Structurei
Secondary structure
3D structure databases
SMRi | P76256 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P76256 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG1214, Bacteria |
HOGENOMi | CLU_064886_2_0_6 |
InParanoidi | P76256 |
PhylomeDBi | P76256 |
Family and domain databases
InterProi | View protein in InterPro IPR043129, ATPase_NBD IPR000905, Gcp-like_dom IPR022496, T6A_TsaB |
PANTHERi | PTHR11735, PTHR11735, 1 hit PTHR11735:SF11, PTHR11735:SF11, 1 hit |
Pfami | View protein in Pfam PF00814, TsaD, 1 hit |
SUPFAMi | SSF53067, SSF53067, 2 hits |
TIGRFAMsi | TIGR03725, T6A_YeaZ, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MRILAIDTAT EACSVALWND GTVNAHFELC PREHTQRILP MVQDILTTSG
60 70 80 90 100
TSLTDINALA YGRGPGSFTG VRIGIGIAQG LALGAELPMI GVSTLMTMAQ
110 120 130 140 150
GAWRKNGATR VLAAIDARMG EVYWAEYQRD ENGIWHGEET EAVLKPEIVH
160 170 180 190 200
ERMQQLSGEW VTVGTGWQAW PDLGKESGLV LRDGEVLLPA AEDMLPIACQ
210 220 230
MFAEGKTVAV EHAEPVYLRN NVAWKKLPGK E
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74877.1 AP009048 Genomic DNA Translation: BAA15611.1 |
PIRi | G64941 |
RefSeqi | NP_416321.1, NC_000913.3 WP_001220966.1, NZ_SSZK01000001.1 |
Genome annotation databases
EnsemblBacteriai | AAC74877; AAC74877; b1807 BAA15611; BAA15611; BAA15611 |
GeneIDi | 946304 |
KEGGi | ecj:JW1796 eco:b1807 |
PATRICi | fig|1411691.4.peg.446 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74877.1 AP009048 Genomic DNA Translation: BAA15611.1 |
PIRi | G64941 |
RefSeqi | NP_416321.1, NC_000913.3 WP_001220966.1, NZ_SSZK01000001.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1OKJ | X-ray | 2.28 | A/B/C/D | 2-231 | [»] | |
4WQ4 | X-ray | 2.33 | C/D | 1-231 | [»] | |
4WQ5 | X-ray | 2.33 | C/D | 1-231 | [»] | |
4YDU | X-ray | 2.33 | C/D | 1-231 | [»] | |
6Z81 | X-ray | 2.31 | C/D | 1-231 | [»] | |
SMRi | P76256 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260340, 15 interactors 850661, 5 interactors |
ComplexPortali | CPX-1094, YgjD-YeaZ-YjeE complex |
DIPi | DIP-11804N |
IntActi | P76256, 12 interactors |
STRINGi | 511145.b1807 |
Proteomic databases
jPOSTi | P76256 |
PaxDbi | P76256 |
PRIDEi | P76256 |
Genome annotation databases
EnsemblBacteriai | AAC74877; AAC74877; b1807 BAA15611; BAA15611; BAA15611 |
GeneIDi | 946304 |
KEGGi | ecj:JW1796 eco:b1807 |
PATRICi | fig|1411691.4.peg.446 |
Organism-specific databases
EchoBASEi | EB3285 |
Phylogenomic databases
eggNOGi | COG1214, Bacteria |
HOGENOMi | CLU_064886_2_0_6 |
InParanoidi | P76256 |
PhylomeDBi | P76256 |
Enzyme and pathway databases
BioCyci | EcoCyc:G6991-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P76256 |
PROi | PR:P76256 |
Family and domain databases
InterProi | View protein in InterPro IPR043129, ATPase_NBD IPR000905, Gcp-like_dom IPR022496, T6A_TsaB |
PANTHERi | PTHR11735, PTHR11735, 1 hit PTHR11735:SF11, PTHR11735:SF11, 1 hit |
Pfami | View protein in Pfam PF00814, TsaD, 1 hit |
SUPFAMi | SSF53067, SSF53067, 2 hits |
TIGRFAMsi | TIGR03725, T6A_YeaZ, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TSAB_ECOLI | |
Accessioni | P76256Primary (citable) accession number: P76256 Secondary accession number(s): O08476, O08477 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | February 1, 1997 | |
Last modified: | February 23, 2022 | |
This is version 161 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families