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Protein

Probable diguanylate cyclase CdgI

Gene

cdgI

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the synthesis of cyclic-di-GMP (c-di-GMP) via the condensation of 2 GTP molecules.By similarity

Caution

Has a EGEVF motif instead of the conserved GG[D/E]EF motif characteristic of active diguanylate cyclases, suggesting that CdgI may be inactive. However, site-directed mutagenesis shows that it is probably a functional diguanylate cyclase.1 Publication

Catalytic activityi

2 GTP = 2 diphosphate + cyclic di-3',5'-guanylate.By similarity

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per monomer.By similarity

Pathwayi: 3',5'-cyclic di-GMP biosynthesis

This protein is involved in the pathway 3',5'-cyclic di-GMP biosynthesis, which is part of Purine metabolism.By similarity
View all proteins of this organism that are known to be involved in the pathway 3',5'-cyclic di-GMP biosynthesis and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi364MagnesiumBy similarity1
Metal bindingi365Magnesium; via carbonyl oxygenBy similarity1
Sitei369Transition state stabilizerSequence analysis1
Binding sitei372SubstrateBy similarity1
Binding sitei377Substrate; via carbonyl oxygenBy similarity1
Binding sitei381SubstrateBy similarity1
Active sitei407Proton acceptorSequence analysis1
Metal bindingi407MagnesiumBy similarity1
Binding sitei427SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

  • negative regulation of bacterial-type flagellum-dependent cell motility Source: EcoCyc

Keywordsi

Molecular functionTransferase
LigandGTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:G6971-MONOMER
UniPathwayiUPA00599

Names & Taxonomyi

Protein namesi
Recommended name:
Probable diguanylate cyclase CdgICurated (EC:2.7.7.65By similarity)
Short name:
DGCCurated
Gene namesi
Name:cdgI1 Publication
Synonyms:yeaI
Ordered Locus Names:b1785, JW1774
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13495 yeaI

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 54CytoplasmicCuratedAdd BLAST54
Transmembranei55 – 75HelicalSequence analysisAdd BLAST21
Topological domaini76 – 89PeriplasmicCuratedAdd BLAST14
Transmembranei90 – 110HelicalSequence analysisAdd BLAST21
Topological domaini111 – 121CytoplasmicCuratedAdd BLAST11
Transmembranei122 – 142HelicalSequence analysisAdd BLAST21
Topological domaini143 – 158PeriplasmicCuratedAdd BLAST16
Transmembranei159 – 179HelicalSequence analysisAdd BLAST21
Topological domaini180 – 193CytoplasmicCuratedAdd BLAST14
Transmembranei194 – 214HelicalSequence analysisAdd BLAST21
Topological domaini215 – 236PeriplasmicCuratedAdd BLAST22
Transmembranei237 – 257HelicalSequence analysisAdd BLAST21
Topological domaini258 – 265CytoplasmicCurated8
Transmembranei266 – 286HelicalSequence analysisAdd BLAST21
Topological domaini287 – 293PeriplasmicCurated7
Transmembranei294 – 314HelicalSequence analysisAdd BLAST21
Topological domaini315 – 491Cytoplasmic1 PublicationAdd BLAST177

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Deletion of the gene increases swimming motility and early biofilm formation.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi407E → A: Abolishes impact on swimming motility. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001690151 – 491Probable diguanylate cyclase CdgIAdd BLAST491

Proteomic databases

PaxDbiP76236
PRIDEiP76236

Expressioni

Inductioni

Induced by 0.3M NaCl in an RpoS-dependent fashion.1 Publication

Interactioni

Subunit structurei

Homodimer.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
htpGP0A6Z33EBI-552525,EBI-369221

Protein-protein interaction databases

BioGridi4260314, 18 interactors
DIPiDIP-11788N
IntActiP76236, 2 interactors
STRINGi316385.ECDH10B_1923

Structurei

3D structure databases

ProteinModelPortaliP76236
SMRiP76236
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini356 – 491GGDEFPROSITE-ProRule annotationAdd BLAST136

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4106KTS Bacteria
COG2199 LUCA
HOGENOMiHOG000118907
InParanoidiP76236
OMAiPGILVCV

Family and domain databases

CDDicd01949 GGDEF, 1 hit
InterProiView protein in InterPro
IPR000160 GGDEF_dom
IPR033424 MASE4
IPR029787 Nucleotide_cyclase
PfamiView protein in Pfam
PF00990 GGDEF, 1 hit
PF17158 MASE4, 1 hit
SMARTiView protein in SMART
SM00267 GGDEF, 1 hit
SUPFAMiSSF55073 SSF55073, 1 hit
TIGRFAMsiTIGR00254 GGDEF, 1 hit
PROSITEiView protein in PROSITE
PS50887 GGDEF, 1 hit

Sequencei

Sequence statusi: Complete.

P76236-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIQSTRISMG LFFKYFLSLT KIDPGQNYIS LPSIKSSTHI ALLFMVSMGT
60 70 80 90 100
QKLKAQSFFI FSLLLTLILF CITTLYNENT NVKLIPQMNY LMVVVALFFL
110 120 130 140 150
NAVIFLFMLM KYFTNKQILP TLILSLAFLS GLIYLVETIV IIHKPINGST
160 170 180 190 200
LIQTKSNDVS IFYIFRQLSF ICLTSLALFC YGKDNILDNN KKKTGILLLA
210 220 230 240 250
LIPFLVFPLL AHNLSSYNAD YSLYVVDYCP DNHTATWGIN YTKILVCLWA
260 270 280 290 300
FLLFFIIMRT RLASELWPLI ALLCLASLCC NLLLLTLDEY NYTIWYISRG
310 320 330 340 350
IEVSSKLFVV SFLIYNIFQE LQLSSKLAVH DVLTNIYNRR YFFNSVESLL
360 370 380 390 400
SRPVVKDFCV MLVDINQFKR INAQWGHRVG DKVLVSIVDI IQQSIRPDDI
410 420 430 440 450
LARLEGEVFG LLFTELNSAQ AKIIAERMRK NVELLTGFSN RYDVPEQMTI
460 470 480 490
SIGTVFSTGD TRNISLVMTE ADKALREAKS EGGNKVIIHH I
Length:491
Mass (Da):56,101
Last modified:February 1, 1997 - v1
Checksum:i2CF33E70FB6551AB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74855.1
AP009048 Genomic DNA Translation: BAA15586.1
PIRiA64939
RefSeqiNP_416299.1, NC_000913.3
WP_000616433.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74855; AAC74855; b1785
BAA15586; BAA15586; BAA15586
GeneIDi946366
KEGGiecj:JW1774
eco:b1785
PATRICifig|511145.12.peg.1858

Similar proteinsi

Entry informationi

Entry nameiCDGI_ECOLI
AccessioniPrimary (citable) accession number: P76236
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 1, 1997
Last modified: April 25, 2018
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health