UniProtKB - P76217 (ASTD_ECOLI)
Protein
N-succinylglutamate 5-semialdehyde dehydrogenase
Gene
astD
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the NAD-dependent reduction of succinylglutamate semialdehyde into succinylglutamate (Probable). Also shows in vitro activity with decanal or succinic semialdehyde as the electron donor and NAD as the electron acceptor. No activity is detected with NADP as the electron acceptor. Therefore, is an aldehyde dehydrogenase with broad substrate specificity (PubMed:15808744).1 Publication1 Publication
Catalytic activityi
- H2O + N-succinyl-L-glutamate 5-semialdehyde + NAD+ = 2 H+ + N-succinyl-L-glutamate + NADH2 PublicationsEC:1.2.1.712 PublicationsThis reaction proceeds in the forward1 Publication direction.
Kineticsi
- KM=91 µM for decanal1 Publication
- KM=160 µM for NAD (at pH 8.5)1 Publication
- KM=1.72 mM for succinic semialdehyde (at pH 8.5)1 Publication
- Vmax=5.0 µmol/min/mg enzyme with decanal as substrate1 Publication
- Vmax=0.69 µmol/min/mg enzyme with succinic semialdehyde as substrate1 Publication
: L-arginine degradation via AST pathway Pathwayi
This protein is involved in step 4 of the subpathway that synthesizes L-glutamate and succinate from L-arginine.1 PublicationProteins known to be involved in the 5 steps of the subpathway in this organism are:
- Arginine N-succinyltransferase (astA), Arginine N-succinyltransferase (astA)
- N-succinylarginine dihydrolase (astB), N-succinylarginine dihydrolase (astB)
- Succinylornithine transaminase (astC), Succinylornithine transaminase (astC)
- N-succinylglutamate 5-semialdehyde dehydrogenase (astD), N-succinylglutamate 5-semialdehyde dehydrogenase (astD)
- Succinylglutamate desuccinylase (astE), Succinylglutamate desuccinylase (astE)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate and succinate from L-arginine, the pathway L-arginine degradation via AST pathway and in Amino-acid degradation.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 243 | By similarity | 1 | |
Active sitei | 277 | By similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 220 – 225 | NADBy similarity | 6 |
GO - Molecular functioni
- aldehyde dehydrogenase (NAD+) activity Source: EcoCyc
- succinylglutamate-semialdehyde dehydrogenase activity Source: UniProtKB-EC
GO - Biological processi
- arginine catabolic process to glutamate Source: UniProtKB-UniRule
- arginine catabolic process to succinate Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Arginine metabolism, Stress response |
Ligand | NAD |
Enzyme and pathway databases
BioCyci | EcoCyc:SUCCGLUALDDEHYD-MONOMER MetaCyc:SUCCGLUALDDEHYD-MONOMER |
BRENDAi | 1.2.1.3, 2026 |
UniPathwayi | UPA00185;UER00282 |
Names & Taxonomyi
Protein namesi | Recommended name: N-succinylglutamate 5-semialdehyde dehydrogenase (EC:1.2.1.712 Publications)Alternative name(s): Succinylglutamic semialdehyde dehydrogenase Short name: SGSD |
Gene namesi | Name:astD Synonyms:ydjU Ordered Locus Names:b1746, JW5282 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000056569 | 1 – 492 | N-succinylglutamate 5-semialdehyde dehydrogenaseAdd BLAST | 492 |
Proteomic databases
jPOSTi | P76217 |
PaxDbi | P76217 |
PRIDEi | P76217 |
Expressioni
Inductioni
By nitrogen starvation, and arginine. Induced at stationary phase by sigma S.2 Publications
Interactioni
Binary interactionsi
P76217
With | #Exp. | IntAct |
---|---|---|
mutH [P06722] | 2 | EBI-545161,EBI-545170 |
Protein-protein interaction databases
BioGRIDi | 4262236, 13 interactors |
DIPi | DIP-9186N |
IntActi | P76217, 1 interactor |
STRINGi | 511145.b1746 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG1012, Bacteria |
HOGENOMi | CLU_005391_1_0_6 |
InParanoidi | P76217 |
PhylomeDBi | P76217 |
Family and domain databases
CDDi | cd07095, ALDH_SGSD_AstD, 1 hit |
Gene3Di | 3.40.309.10, 1 hit 3.40.605.10, 1 hit |
HAMAPi | MF_01174, Aldedh_AstD, 1 hit |
InterProi | View protein in InterPro IPR016161, Ald_DH/histidinol_DH IPR016163, Ald_DH_C IPR016160, Ald_DH_CS_CYS IPR029510, Ald_DH_CS_GLU IPR016162, Ald_DH_N IPR015590, Aldehyde_DH_dom IPR017649, SuccinylGlu_semiald_DH_AstD |
PANTHERi | PTHR11699:SF197, PTHR11699:SF197, 1 hit |
Pfami | View protein in Pfam PF00171, Aldedh, 1 hit |
SUPFAMi | SSF53720, SSF53720, 1 hit |
TIGRFAMsi | TIGR03240, arg_catab_astD, 1 hit |
PROSITEi | View protein in PROSITE PS00070, ALDEHYDE_DEHYDR_CYS, 1 hit PS00687, ALDEHYDE_DEHYDR_GLU, 1 hit |
i Sequence
Sequence statusi: Complete.
P76217-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTLWINGDWI TGQGASRVKR NPVSGEVLWQ GNDADAAQVE QACRAARAAF
60 70 80 90 100
PRWARLSFAE RHAVVERFAA LLESNKAELT AIIARETGKP RWEAATEVTA
110 120 130 140 150
MINKIAISIK AYHVRTGEQR SEMPDGAASL RHRPHGVLAV FGPYNFPGHL
160 170 180 190 200
PNGHIVPALL AGNTIIFKPS ELTPWSGEAV MRLWQQAGLP PGVLNLVQGG
210 220 230 240 250
RETGQALSAL EDLDGLLFTG SANTGYQLHR QLSGQPEKIL ALEMGGNNPL
260 270 280 290 300
IIDEVADIDA AVHLTIQSAF VTAGQRCTCA RRLLLKSGAQ GDAFLARLVA
310 320 330 340 350
VSQRLTPGNW DDEPQPFIGG LISEQAAQQV VTAWQQLEAM GGRPLLAPRL
360 370 380 390 400
LQAGTSLLTP GIIEMTGVAG VPDEEVFGPL LRVWRYDTFD EAIRMANNTR
410 420 430 440 450
FGLSCGLVSP EREKFDQLLL EARAGIVNWN KPLTGAASTA PFGGIGASGN
460 470 480 490
HRPSAWYAAD YCAWPMASLE SDSLTLPATL NPGLDFSDEV VR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74816.1 AP009048 Genomic DNA Translation: BAA15538.2 |
PIRi | B64934 |
RefSeqi | NP_416260.1, NC_000913.3 WP_000177206.1, NZ_SSZK01000001.1 |
Genome annotation databases
EnsemblBacteriai | AAC74816; AAC74816; b1746 BAA15538; BAA15538; BAA15538 |
GeneIDi | 946260 |
KEGGi | ecj:JW5282 eco:b1746 |
PATRICi | fig|1411691.4.peg.510 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74816.1 AP009048 Genomic DNA Translation: BAA15538.2 |
PIRi | B64934 |
RefSeqi | NP_416260.1, NC_000913.3 WP_000177206.1, NZ_SSZK01000001.1 |
3D structure databases
SMRi | P76217 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4262236, 13 interactors |
DIPi | DIP-9186N |
IntActi | P76217, 1 interactor |
STRINGi | 511145.b1746 |
Proteomic databases
jPOSTi | P76217 |
PaxDbi | P76217 |
PRIDEi | P76217 |
Genome annotation databases
EnsemblBacteriai | AAC74816; AAC74816; b1746 BAA15538; BAA15538; BAA15538 |
GeneIDi | 946260 |
KEGGi | ecj:JW5282 eco:b1746 |
PATRICi | fig|1411691.4.peg.510 |
Organism-specific databases
EchoBASEi | EB3753 |
Phylogenomic databases
eggNOGi | COG1012, Bacteria |
HOGENOMi | CLU_005391_1_0_6 |
InParanoidi | P76217 |
PhylomeDBi | P76217 |
Enzyme and pathway databases
UniPathwayi | UPA00185;UER00282 |
BioCyci | EcoCyc:SUCCGLUALDDEHYD-MONOMER MetaCyc:SUCCGLUALDDEHYD-MONOMER |
BRENDAi | 1.2.1.3, 2026 |
Miscellaneous databases
PROi | PR:P76217 |
Family and domain databases
CDDi | cd07095, ALDH_SGSD_AstD, 1 hit |
Gene3Di | 3.40.309.10, 1 hit 3.40.605.10, 1 hit |
HAMAPi | MF_01174, Aldedh_AstD, 1 hit |
InterProi | View protein in InterPro IPR016161, Ald_DH/histidinol_DH IPR016163, Ald_DH_C IPR016160, Ald_DH_CS_CYS IPR029510, Ald_DH_CS_GLU IPR016162, Ald_DH_N IPR015590, Aldehyde_DH_dom IPR017649, SuccinylGlu_semiald_DH_AstD |
PANTHERi | PTHR11699:SF197, PTHR11699:SF197, 1 hit |
Pfami | View protein in Pfam PF00171, Aldedh, 1 hit |
SUPFAMi | SSF53720, SSF53720, 1 hit |
TIGRFAMsi | TIGR03240, arg_catab_astD, 1 hit |
PROSITEi | View protein in PROSITE PS00070, ALDEHYDE_DEHYDR_CYS, 1 hit PS00687, ALDEHYDE_DEHYDR_GLU, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ASTD_ECOLI | |
Accessioni | P76217Primary (citable) accession number: P76217 Secondary accession number(s): P78169, P78170 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 15, 1998 |
Last sequence update: | February 1, 1997 | |
Last modified: | December 2, 2020 | |
This is version 160 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families