UniProtKB - P76215 (ASTE_ECOLI)
Protein
Succinylglutamate desuccinylase
Gene
astE
Organism
Escherichia coli (strain K12)
Status
Functioni
Transforms N2-succinylglutamate into succinate and glutamate.1 Publication
Catalytic activityi
- EC:3.5.1.96
Cofactori
Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity
: L-arginine degradation via AST pathway Pathwayi
This protein is involved in step 5 of the subpathway that synthesizes L-glutamate and succinate from L-arginine.Proteins known to be involved in the 5 steps of the subpathway in this organism are:
- Arginine N-succinyltransferase (astA), Arginine N-succinyltransferase (astA)
- N-succinylarginine dihydrolase (astB), N-succinylarginine dihydrolase (astB)
- Succinylornithine transaminase (astC), Succinylornithine transaminase (astC)
- N-succinylglutamate 5-semialdehyde dehydrogenase (astD), N-succinylglutamate 5-semialdehyde dehydrogenase (astD)
- Succinylglutamate desuccinylase (astE), Succinylglutamate desuccinylase (astE)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate and succinate from L-arginine, the pathway L-arginine degradation via AST pathway and in Amino-acid degradation.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 53 | ZincBy similarity | 1 | |
Metal bindingi | 56 | ZincBy similarity | 1 | |
Metal bindingi | 147 | ZincBy similarity | 1 | |
Active sitei | 210 | Sequence analysis | 1 |
GO - Molecular functioni
- hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides Source: GO_Central
- hydrolase activity, acting on ester bonds Source: UniProtKB-UniRule
- succinylglutamate desuccinylase activity Source: EcoCyc
- zinc ion binding Source: UniProtKB-UniRule
GO - Biological processi
- arginine catabolic process to glutamate Source: UniProtKB-UniRule
- arginine catabolic process to succinate Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Hydrolase |
Biological process | Arginine metabolism, Stress response |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
BioCyci | EcoCyc:SUCCGLUDESUCC-MONOMER MetaCyc:SUCCGLUDESUCC-MONOMER |
BRENDAi | 3.5.1.96, 2026 |
UniPathwayi | UPA00185;UER00283 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:astE Synonyms:ydjS Ordered Locus Names:b1744, JW1733 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000174639 | 1 – 322 | Succinylglutamate desuccinylaseAdd BLAST | 322 |
Proteomic databases
jPOSTi | P76215 |
PaxDbi | P76215 |
PRIDEi | P76215 |
Expressioni
Inductioni
By nitrogen starvation, and arginine. Induced at stationary phase by sigma S.1 Publication
Interactioni
Binary interactionsi
P76215
With | #Exp. | IntAct |
---|---|---|
acrR [P0ACS9] | 3 | EBI-1121806,EBI-1117360 |
Protein-protein interaction databases
BioGRIDi | 4262239, 18 interactors 850616, 4 interactors |
IntActi | P76215, 10 interactors |
STRINGi | 511145.b1744 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P76215 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P76215 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG2988, Bacteria |
HOGENOMi | CLU_071608_0_0_6 |
InParanoidi | P76215 |
Family and domain databases
CDDi | cd03855, M14_ASTE, 1 hit |
HAMAPi | MF_00767, Arg_catab_AstE, 1 hit |
InterProi | View protein in InterPro IPR007036, Aste_AspA IPR016681, SuccinylGlu_desuccinylase |
Pfami | View protein in Pfam PF04952, AstE_AspA, 1 hit |
PIRSFi | PIRSF017020, AstE, 1 hit |
TIGRFAMsi | TIGR03242, arg_catab_astE, 1 hit |
i Sequence
Sequence statusi: Complete.
P76215-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDNFLALTLT GKKPVITERE INGVRWRWLG DGVLELTPLT PPQGALVISA
60 70 80 90 100
GIHGNETAPV EMLDALLGAI SHGEIPLRWR LLVILGNPPA LKQGKRYCHS
110 120 130 140 150
DMNRMFGGRW QLFAESGETC RARELEQCLE DFYDQGKESV RWHLDLHTAI
160 170 180 190 200
RGSLHPQFGV LPQRDIPWDE KFLTWLGAAG LEALVFHQEP GGTFTHFSAR
210 220 230 240 250
HFGALACTLE LGKALPFGQN DLRQFAVTAS AIAALLSGES VGIVRTPPLR
260 270 280 290 300
YRVVSQITRH SPSFEMHMAS DTLNFMPFEK GTLLAQDGEE RFTVTHDVEY
310 320
VLFPNPLVAL GLRAGLMLEK IS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74814.1 AP009048 Genomic DNA Translation: BAE76515.1 |
PIRi | H64933 |
RefSeqi | NP_416258.1, NC_000913.3 WP_000368506.1, NZ_SSZK01000001.1 |
Genome annotation databases
EnsemblBacteriai | AAC74814; AAC74814; b1744 BAE76515; BAE76515; BAE76515 |
GeneIDi | 57731140 946256 |
KEGGi | ecj:JW1733 eco:b1744 |
PATRICi | fig|1411691.4.peg.512 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74814.1 AP009048 Genomic DNA Translation: BAE76515.1 |
PIRi | H64933 |
RefSeqi | NP_416258.1, NC_000913.3 WP_000368506.1, NZ_SSZK01000001.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1YW6 | X-ray | 3.10 | A/B | 1-322 | [»] | |
SMRi | P76215 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262239, 18 interactors 850616, 4 interactors |
IntActi | P76215, 10 interactors |
STRINGi | 511145.b1744 |
Proteomic databases
jPOSTi | P76215 |
PaxDbi | P76215 |
PRIDEi | P76215 |
Genome annotation databases
EnsemblBacteriai | AAC74814; AAC74814; b1744 BAE76515; BAE76515; BAE76515 |
GeneIDi | 57731140 946256 |
KEGGi | ecj:JW1733 eco:b1744 |
PATRICi | fig|1411691.4.peg.512 |
Organism-specific databases
EchoBASEi | EB3751 |
Phylogenomic databases
eggNOGi | COG2988, Bacteria |
HOGENOMi | CLU_071608_0_0_6 |
InParanoidi | P76215 |
Enzyme and pathway databases
UniPathwayi | UPA00185;UER00283 |
BioCyci | EcoCyc:SUCCGLUDESUCC-MONOMER MetaCyc:SUCCGLUDESUCC-MONOMER |
BRENDAi | 3.5.1.96, 2026 |
Miscellaneous databases
EvolutionaryTracei | P76215 |
PROi | PR:P76215 |
Family and domain databases
CDDi | cd03855, M14_ASTE, 1 hit |
HAMAPi | MF_00767, Arg_catab_AstE, 1 hit |
InterProi | View protein in InterPro IPR007036, Aste_AspA IPR016681, SuccinylGlu_desuccinylase |
Pfami | View protein in Pfam PF04952, AstE_AspA, 1 hit |
PIRSFi | PIRSF017020, AstE, 1 hit |
TIGRFAMsi | TIGR03242, arg_catab_astE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ASTE_ECOLI | |
Accessioni | P76215Primary (citable) accession number: P76215 Secondary accession number(s): Q2MB41 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | February 1, 1997 | |
Last modified: | April 7, 2021 | |
This is version 151 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families