UniProtKB - P76194 (SUFE_ECOLI)
Protein
Cysteine desulfuration protein SufE
Gene
sufE
Organism
Escherichia coli (strain K12)
Status
Functioni
Participates in cysteine desulfuration mediated by SufS. Cysteine desulfuration mobilizes sulfur from L-cysteine to yield L-alanine and constitutes an essential step in sulfur metabolism for biosynthesis of a variety of sulfur-containing biomolecules. Functions as a sulfur acceptor for SufS, by mediating the direct transfer of the sulfur atom from the S-sulfanylcysteine of SufS, an intermediate product of cysteine desulfuration process. Together with the SufBCD complex, it thereby enhances up to 50-fold, the cysteine desulfurase activity of SufS. Component of the suf operon, which is activated and required under specific conditions such as oxidative stress and iron limitation. Does not affect the selenocysteine lyase activity of SufS.3 Publications
: iron-sulfur cluster biosynthesis Pathwayi
This protein is involved in the pathway iron-sulfur cluster biosynthesis, which is part of Cofactor biosynthesis.View all proteins of this organism that are known to be involved in the pathway iron-sulfur cluster biosynthesis and in Cofactor biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 51 | Cysteine persulfide intermediateCurated | 1 |
GO - Molecular functioni
- enzyme activator activity Source: EcoCyc
- sulfur carrier activity Source: EcoCyc
GO - Biological processi
- iron-sulfur cluster assembly Source: InterPro
- positive regulation of catalytic activity Source: EcoCyc
- response to oxidative stress Source: EcoCyc
- sulfur incorporation into metallo-sulfur cluster Source: EcoCyc
Enzyme and pathway databases
BioCyci | EcoCyc:G6905-MONOMER MetaCyc:G6905-MONOMER |
UniPathwayi | UPA00266 |
Names & Taxonomyi
Protein namesi | Recommended name: Cysteine desulfuration protein SufE |
Gene namesi | Name:sufE Synonyms:ynhA Ordered Locus Names:b1679, JW1669 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm By similarity
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 51 | C → S: Abolishes cysteine desulfurase activity. 3 Publications | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000202124 | 1 – 138 | Cysteine desulfuration protein SufEAdd BLAST | 138 |
Proteomic databases
jPOSTi | P76194 |
PaxDbi | P76194 |
PRIDEi | P76194 |
Expressioni
Inductioni
Suf operon is under both the Fe-dependent Fur repressor and the oxidative stress dependent OxyR activator.
Interactioni
Subunit structurei
Homodimer.
Interacts with SufS.
3 PublicationsBinary interactionsi
P76194
With | #Exp. | IntAct |
---|---|---|
sufS [P77444] | 3 | EBI-1124973,EBI-1124981 |
Protein-protein interaction databases
BioGRIDi | 4260278, 38 interactors |
ComplexPortali | CPX-2125, SufE complex |
DIPi | DIP-10941N |
IntActi | P76194, 9 interactors |
STRINGi | 511145.b1679 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P76194 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P76194 |
Family & Domainsi
Sequence similaritiesi
Belongs to the SufE family.Curated
Phylogenomic databases
eggNOGi | COG2166, Bacteria |
HOGENOMi | CLU_124502_1_1_6 |
InParanoidi | P76194 |
PhylomeDBi | P76194 |
Family and domain databases
HAMAPi | MF_01832, SufE, 1 hit |
InterProi | View protein in InterPro IPR023939, Cysteine_desulfuration_SufE IPR003808, Fe-S_metab-assoc_dom |
PANTHERi | PTHR43597, PTHR43597, 1 hit |
Pfami | View protein in Pfam PF02657, SufE, 1 hit |
i Sequence
Sequence statusi: Complete.
P76194-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MALLPDKEKL LRNFLRCANW EEKYLYIIEL GQRLPELRDE DRSPQNSIQG
60 70 80 90 100
CQSQVWIVMR QNAQGIIELQ GDSDAAIVKG LIAVVFILYD QMTPQDIVNF
110 120 130
DVRPWFEKMA LTQHLTPSRS QGLEAMIRAI RAKAAALS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74749.1 AP009048 Genomic DNA Translation: BAE76500.1 |
PIRi | G64925 |
RefSeqi | NP_416194.1, NC_000913.3 WP_001196530.1, NZ_STEB01000003.1 |
Genome annotation databases
EnsemblBacteriai | AAC74749; AAC74749; b1679 BAE76500; BAE76500; BAE76500 |
GeneIDi | 946173 |
KEGGi | ecj:JW1669 eco:b1679 |
PATRICi | fig|1411691.4.peg.579 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74749.1 AP009048 Genomic DNA Translation: BAE76500.1 |
PIRi | G64925 |
RefSeqi | NP_416194.1, NC_000913.3 WP_001196530.1, NZ_STEB01000003.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1MZG | X-ray | 2.00 | A/B | 1-138 | [»] | |
SMRi | P76194 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260278, 38 interactors |
ComplexPortali | CPX-2125, SufE complex |
DIPi | DIP-10941N |
IntActi | P76194, 9 interactors |
STRINGi | 511145.b1679 |
Proteomic databases
jPOSTi | P76194 |
PaxDbi | P76194 |
PRIDEi | P76194 |
Genome annotation databases
EnsemblBacteriai | AAC74749; AAC74749; b1679 BAE76500; BAE76500; BAE76500 |
GeneIDi | 946173 |
KEGGi | ecj:JW1669 eco:b1679 |
PATRICi | fig|1411691.4.peg.579 |
Organism-specific databases
EchoBASEi | EB3719 |
Phylogenomic databases
eggNOGi | COG2166, Bacteria |
HOGENOMi | CLU_124502_1_1_6 |
InParanoidi | P76194 |
PhylomeDBi | P76194 |
Enzyme and pathway databases
UniPathwayi | UPA00266 |
BioCyci | EcoCyc:G6905-MONOMER MetaCyc:G6905-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P76194 |
PROi | PR:P76194 |
Family and domain databases
HAMAPi | MF_01832, SufE, 1 hit |
InterProi | View protein in InterPro IPR023939, Cysteine_desulfuration_SufE IPR003808, Fe-S_metab-assoc_dom |
PANTHERi | PTHR43597, PTHR43597, 1 hit |
Pfami | View protein in Pfam PF02657, SufE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SUFE_ECOLI | |
Accessioni | P76194Primary (citable) accession number: P76194 Secondary accession number(s): Q2MB56 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | February 1, 1997 | |
Last modified: | December 2, 2020 | |
This is version 142 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Uncharacterized protein families (UPF)
List of uncharacterized protein family (UPF) entries - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families