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UniProtKB - P76114 (MCBR_ECOLI)
Protein
HTH-type transcriptional regulator McbR
Gene
mcbR
Organism
Escherichia coli (strain K12)
Status
Functioni
Important for biofilm formation. Represses expression of McbA by binding to its promoter region, which prevents colanic acid overproduction and mucoidy.
1 PublicationRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 37 – 56 | H-T-H motifPROSITE-ProRule annotationAdd BLAST | 20 |
GO - Molecular functioni
- DNA binding Source: UniProtKB-KW
- DNA-binding transcription factor activity Source: EcoCyc
GO - Biological processi
- negative regulation of transcription, DNA-templated Source: EcoCyc
Keywordsi
Molecular function | DNA-binding, Repressor |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
BioCyci | EcoCyc:G6761-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: HTH-type transcriptional regulator McbR |
Gene namesi | Name:mcbR Synonyms:yncC Ordered Locus Names:b1450, JW1445 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Disruption of this gene induces 30 genes and represses 10 genes more than fourfold. It decreases biofilm formation, increases extracellular indole, leads to colanic acid overproduction and elicits mucoidy.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000050686 | 1 – 221 | HTH-type transcriptional regulator McbRAdd BLAST | 221 |
Proteomic databases
jPOSTi | P76114 |
PaxDbi | P76114 |
PRIDEi | P76114 |
Expressioni
Inductioni
Induced by LsrR, LsrK, MqsR and TqsA. Repressed by LuxS and YgiV.1 Publication
Interactioni
Binary interactionsi
P76114
With | #Exp. | IntAct |
---|---|---|
rplC [P60438] | 2 | EBI-560824,EBI-542200 |
Protein-protein interaction databases
BioGRIDi | 4260196, 3 interactors 850375, 2 interactors |
DIPi | DIP-12749N |
IntActi | P76114, 11 interactors |
STRINGi | 511145.b1450 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 10 – 77 | HTH gntR-typePROSITE-ProRule annotationAdd BLAST | 68 |
Phylogenomic databases
eggNOGi | COG1802, Bacteria |
HOGENOMi | CLU_017584_5_4_6 |
InParanoidi | P76114 |
OMAi | SLTMQVE |
PhylomeDBi | P76114 |
Family and domain databases
Gene3Di | 1.10.10.10, 1 hit 1.20.120.530, 1 hit |
InterProi | View protein in InterPro IPR011711, GntR_C IPR008920, TF_FadR/GntR_C IPR000524, Tscrpt_reg_HTH_GntR IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
Pfami | View protein in Pfam PF07729, FCD, 1 hit PF00392, GntR, 1 hit |
SMARTi | View protein in SMART SM00895, FCD, 1 hit SM00345, HTH_GNTR, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit SSF48008, SSF48008, 1 hit |
PROSITEi | View protein in PROSITE PS50949, HTH_GNTR, 1 hit |
i Sequence
Sequence statusi: Complete.
P76114-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPGTGKMKHV SLTLQVENDL KHQLSIGALK PGARLITKNL AEQLGMSITP
60 70 80 90 100
VREALLRLVS VNALSVAPAQ AFTVPEVGKR QLDEINRIRY ELELMAVALA
110 120 130 140 150
VENLTPQDLA ELQELLEKLQ QAQEKGDMEQ IINVNRLFRL AIYHRSNMPI
160 170 180 190 200
LCEMIEQLWV RMGPGLHYLY EAINPAELRE HIENYHLLLA ALKAKDKEGC
210 220
RHCLAEIMQQ NIAILYQQYN R
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74532.2 AP009048 Genomic DNA Translation: BAA15082.1 |
PIRi | E64897 |
RefSeqi | NP_415967.2, NC_000913.3 WP_001300742.1, NZ_STEB01000043.1 |
Genome annotation databases
EnsemblBacteriai | AAC74532; AAC74532; b1450 BAA15082; BAA15082; BAA15082 |
GeneIDi | 946014 |
KEGGi | ecj:JW1445 eco:b1450 |
PATRICi | fig|1411691.4.peg.818 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74532.2 AP009048 Genomic DNA Translation: BAA15082.1 |
PIRi | E64897 |
RefSeqi | NP_415967.2, NC_000913.3 WP_001300742.1, NZ_STEB01000043.1 |
3D structure databases
AlphaFoldDBi | P76114 |
SMRi | P76114 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4260196, 3 interactors 850375, 2 interactors |
DIPi | DIP-12749N |
IntActi | P76114, 11 interactors |
STRINGi | 511145.b1450 |
Proteomic databases
jPOSTi | P76114 |
PaxDbi | P76114 |
PRIDEi | P76114 |
Genome annotation databases
EnsemblBacteriai | AAC74532; AAC74532; b1450 BAA15082; BAA15082; BAA15082 |
GeneIDi | 946014 |
KEGGi | ecj:JW1445 eco:b1450 |
PATRICi | fig|1411691.4.peg.818 |
Organism-specific databases
EchoBASEi | EB3535 |
Phylogenomic databases
eggNOGi | COG1802, Bacteria |
HOGENOMi | CLU_017584_5_4_6 |
InParanoidi | P76114 |
OMAi | SLTMQVE |
PhylomeDBi | P76114 |
Enzyme and pathway databases
BioCyci | EcoCyc:G6761-MONOMER |
Miscellaneous databases
PROi | PR:P76114 |
Family and domain databases
Gene3Di | 1.10.10.10, 1 hit 1.20.120.530, 1 hit |
InterProi | View protein in InterPro IPR011711, GntR_C IPR008920, TF_FadR/GntR_C IPR000524, Tscrpt_reg_HTH_GntR IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
Pfami | View protein in Pfam PF07729, FCD, 1 hit PF00392, GntR, 1 hit |
SMARTi | View protein in SMART SM00895, FCD, 1 hit SM00345, HTH_GNTR, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit SSF48008, SSF48008, 1 hit |
PROSITEi | View protein in PROSITE PS50949, HTH_GNTR, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MCBR_ECOLI | |
Accessioni | P76114Primary (citable) accession number: P76114 Secondary accession number(s): P77347 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1999 |
Last sequence update: | July 15, 1999 | |
Last modified: | May 25, 2022 | |
This is version 150 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |