UniProtKB - P76113 (CURA_ECOLI)
Protein
NADPH-dependent curcumin reductase
Gene
curA
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the metal-independent reduction of curcumin to dihydrocurcumin (DHC) as an intermediate product, followed by further reduction to tetrahydrocurcumin (THC) as an end product. It also acts on 3-octene-2-one, 3-hepten-2-one, resveratrol, and trans-2-octenal.1 Publication
Catalytic activityi
- EC:1.3.1.n31 Publication
Activity regulationi
Inhibited by thiol-specific reagents (p-chloromercuribenzoate and 5,5'-dithio-bis-2-nitrobenzoate).1 Publication
Kineticsi
- KM=29 µM for curcumin1 Publication
- Vmax=9.35 µmol/min/mg enzyme1 Publication
pH dependencei
Optimum pH is 5.9. The enzyme is most stable in the pH range of 4.5 to 12.0, with 90% of the initial activity being retained even at pH 12.0.1 Publication
Temperature dependencei
Optimum temperature is 35 degrees Celsius. The enzyme is most stable from 10 to 50 degrees Celsius.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 51 | NADPBy similarity | 1 | |
Binding sitei | 186 | NADPBy similarity | 1 | |
Binding sitei | 225 | NADPBy similarity | 1 | |
Binding sitei | 333 | NADPBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 160 – 163 | NADPBy similarity | 4 | |
Nucleotide bindingi | 182 – 186 | NADPBy similarity | 5 | |
Nucleotide bindingi | 247 – 253 | NADPBy similarity | 7 | |
Nucleotide bindingi | 282 – 284 | NADPBy similarity | 3 |
GO - Molecular functioni
- oxidoreductase activity Source: UniProtKB-KW
Keywordsi
Molecular function | Oxidoreductase |
Ligand | NADP |
Enzyme and pathway databases
BioCyci | EcoCyc:G6760-MONOMER MetaCyc:G6760-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: NADPH-dependent curcumin reductase (EC:1.3.1.n3)Alternative name(s): NADPH-dependent curcumin/dihydrocurcumin reductase |
Gene namesi | Name:curA Synonyms:yncB Ordered Locus Names:b1449, JW5907 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 247 | C → A: Exhibits a reduction of about 50% in activity, but is not inhibited by 5,5'-dithio-bis-2-nitrobenzoate. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000218075 | 1 – 345 | NADPH-dependent curcumin reductaseAdd BLAST | 345 |
Proteomic databases
jPOSTi | P76113 |
PaxDbi | P76113 |
PRIDEi | P76113 |
Interactioni
Subunit structurei
Homodimer.
1 PublicationProtein-protein interaction databases
BioGRIDi | 4260195, 14 interactors |
DIPi | DIP-12748N |
STRINGi | 511145.b1449 |
Family & Domainsi
Sequence similaritiesi
Belongs to the NADP-dependent oxidoreductase L4BD family.Curated
Phylogenomic databases
eggNOGi | COG2130, Bacteria |
InParanoidi | P76113 |
PhylomeDBi | P76113 |
Family and domain databases
InterProi | View protein in InterPro IPR013149, ADH_C IPR041694, ADH_N_2 IPR011032, GroES-like_sf IPR036291, NAD(P)-bd_dom_sf IPR020843, PKS_ER |
Pfami | View protein in Pfam PF16884, ADH_N_2, 1 hit PF00107, ADH_zinc_N, 1 hit |
SMARTi | View protein in SMART SM00829, PKS_ER, 1 hit |
SUPFAMi | SSF50129, SSF50129, 2 hits SSF51735, SSF51735, 1 hit |
i Sequence
Sequence statusi: Complete.
P76113-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGQQKQRNRR WVLASRPHGA PVPENFRLEE DDVATPGEGQ VLLRTVYLSL
60 70 80 90 100
DPYMRGRMSD EPSYSPPVDI GGVMVGGTVS RVVESNHPDY QSGDWVLGYS
110 120 130 140 150
GWQDYDISSG DDLVKLGDHP QNPSWSLGVL GMPGFTAYMG LLDIGQPKEG
160 170 180 190 200
ETLVVAAATG PVGATVGQIG KLKGCRVVGV AGGAEKCRHA TEVLGFDVCL
210 220 230 240 250
DHHADDFAEQ LAKACPKGID IYYENVGGKV FDAVLPLLNT SARIPVCGLV
260 270 280 290 300
SSYNATELPP GPDRLPLLMA TVLKKRIRLQ GFIIAQDYGH RIHEFQREMG
310 320 330 340
QWVKEDKIHY REEITDGLEN APQTFIGLLK GKNFGKVVIR VAGDD
Mass spectrometryi
Molecular mass is 37513 Da. Determined by MALDI. 1 Publication
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74531.4 AP009048 Genomic DNA Translation: BAA15081.1 |
PIRi | D64897 |
RefSeqi | NP_415966.6, NC_000913.3 WP_000531462.1, NZ_SSZK01000021.1 |
Genome annotation databases
EnsemblBacteriai | AAC74531; AAC74531; b1449 BAA15081; BAA15081; BAA15081 |
GeneIDi | 946012 |
KEGGi | ecj:JW5907 eco:b1449 |
PATRICi | fig|1411691.4.peg.819 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74531.4 AP009048 Genomic DNA Translation: BAA15081.1 |
PIRi | D64897 |
RefSeqi | NP_415966.6, NC_000913.3 WP_000531462.1, NZ_SSZK01000021.1 |
3D structure databases
SMRi | P76113 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4260195, 14 interactors |
DIPi | DIP-12748N |
STRINGi | 511145.b1449 |
Proteomic databases
jPOSTi | P76113 |
PaxDbi | P76113 |
PRIDEi | P76113 |
Genome annotation databases
EnsemblBacteriai | AAC74531; AAC74531; b1449 BAA15081; BAA15081; BAA15081 |
GeneIDi | 946012 |
KEGGi | ecj:JW5907 eco:b1449 |
PATRICi | fig|1411691.4.peg.819 |
Organism-specific databases
EchoBASEi | EB3534 |
Phylogenomic databases
eggNOGi | COG2130, Bacteria |
InParanoidi | P76113 |
PhylomeDBi | P76113 |
Enzyme and pathway databases
BioCyci | EcoCyc:G6760-MONOMER MetaCyc:G6760-MONOMER |
Miscellaneous databases
PROi | PR:P76113 |
Family and domain databases
InterProi | View protein in InterPro IPR013149, ADH_C IPR041694, ADH_N_2 IPR011032, GroES-like_sf IPR036291, NAD(P)-bd_dom_sf IPR020843, PKS_ER |
Pfami | View protein in Pfam PF16884, ADH_N_2, 1 hit PF00107, ADH_zinc_N, 1 hit |
SMARTi | View protein in SMART SM00829, PKS_ER, 1 hit |
SUPFAMi | SSF50129, SSF50129, 2 hits SSF51735, SSF51735, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CURA_ECOLI | |
Accessioni | P76113Primary (citable) accession number: P76113 Secondary accession number(s): P78255 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 24, 2009 | |
Last modified: | December 2, 2020 | |
This is version 138 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families