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UniProtKB - P76085 (PAAK_ECOLI)
Protein
Phenylacetate-coenzyme A ligase
Gene
paaK
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the activation of phenylacetic acid (PA) to phenylacetyl-CoA (PA-CoA).
2 PublicationsCatalytic activityi
: phenylacetate degradation Pathwayi
This protein is involved in the pathway phenylacetate degradation, which is part of Aromatic compound metabolism.1 PublicationView all proteins of this organism that are known to be involved in the pathway phenylacetate degradation and in Aromatic compound metabolism.
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- CoA-ligase activity Source: EcoliWiki
- phenylacetate-CoA ligase activity Source: EcoliWiki
GO - Biological processi
- phenylacetate catabolic process Source: UniProtKB
Keywordsi
Molecular function | Ligase |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:G6719-MONOMER |
UniPathwayi | UPA00930 |
Names & Taxonomyi
Protein namesi | Recommended name: Phenylacetate-coenzyme A ligase (EC:6.2.1.301 Publication)Alternative name(s): Phenylacetyl-CoA ligase Short name: PA-CoA ligase |
Gene namesi | Name:paaK Ordered Locus Names:b1398, JW5218 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000058164 | 1 – 437 | Phenylacetate-coenzyme A ligaseAdd BLAST | 437 |
Proteomic databases
PaxDbi | P76085 |
PRIDEi | P76085 |
Expressioni
Inductioni
Activated by cAMP receptor protein (CRP), integration host factor (IHF) and by phenylacetyl-coenzyme A that prevents PaaX from binding its target sequences. Inhibited by PaaX.2 Publications
Interactioni
Subunit structurei
Monomer.
By similarityProtein-protein interaction databases
BioGRIDi | 4263013, 319 interactors |
DIPi | DIP-10430N |
STRINGi | 511145.b1398 |
Family & Domainsi
Sequence similaritiesi
Belongs to the phenylacetyl-CoA ligase family.Curated
Phylogenomic databases
eggNOGi | COG1541, Bacteria |
HOGENOMi | CLU_035301_1_1_6 |
InParanoidi | P76085 |
PhylomeDBi | P76085 |
Family and domain databases
CDDi | cd05913, PaaK, 1 hit |
Gene3Di | 3.30.300.30, 1 hit 3.40.50.12780, 1 hit |
InterProi | View protein in InterPro IPR045851, AMP-bd_C_sf IPR028154, AMP-dep_Lig_C IPR000873, AMP-dep_Synth/Lig IPR042099, ANL_N_sf IPR011880, PA_CoA_ligase |
Pfami | View protein in Pfam PF00501, AMP-binding, 1 hit PF14535, AMP-binding_C_2, 1 hit |
PIRSFi | PIRSF006444, PaaK, 1 hit |
TIGRFAMsi | TIGR02155, PA_CoA_ligase, 1 hit |
i Sequence
Sequence statusi: Complete.
P76085-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MITNTKLDPI ETASVDELQA LQTQRLKWTL KHAYENVPMY RRKFDAAGVH
60 70 80 90 100
PDDFRELSDL RKFPCTTKQD LRDNYPFDTF AVPMEQVVRI HASSGTTGKP
110 120 130 140 150
TVVGYTQNDI DNWANIVARS LRAAGGSPKD KIHVAYGYGL FTGGLGAHYG
160 170 180 190 200
AERLGATVIP MSGGQTEKQA QLIRDFQPDM IMVTPSYCLN LIEELERQLG
210 220 230 240 250
GDASGCSLRV GVFGAEPWTQ AMRKEIERRL GITALDIYGL SEVMGPGVAM
260 270 280 290 300
ECLETTDGPT IWEDHFYPEI VNPHDGTPLA DGEHGELLFT TLTKEALPVI
310 320 330 340 350
RYRTRDLTRL LPGTARTMRR MDRISGRSDD MLIIRGVNVF PSQLEEEIVK
360 370 380 390 400
FEHLSPHYQL EVNRRGHLDS LSVKVELKES SLTLTHEQRC QVCHQLRHRI
410 420 430
KSMVGISTDV MIVNCGSIPR SEGKACRVFD LRNIVGA
Sequence cautioni
The sequence CAA66100 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 127 | S → T in strain: W. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X97452 Genomic DNA Translation: CAA66100.1 Different initiation. U00096 Genomic DNA Translation: AAC74480.1 AP009048 Genomic DNA Translation: BAE76428.1 |
PIRi | A64891 |
RefSeqi | NP_415916.1, NC_000913.3 WP_000632280.1, NZ_SSZK01000012.1 |
Genome annotation databases
EnsemblBacteriai | AAC74480; AAC74480; b1398 BAE76428; BAE76428; BAE76428 |
GeneIDi | 945963 |
KEGGi | ecj:JW5218 eco:b1398 |
PATRICi | fig|1411691.4.peg.873 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X97452 Genomic DNA Translation: CAA66100.1 Different initiation. U00096 Genomic DNA Translation: AAC74480.1 AP009048 Genomic DNA Translation: BAE76428.1 |
PIRi | A64891 |
RefSeqi | NP_415916.1, NC_000913.3 WP_000632280.1, NZ_SSZK01000012.1 |
3D structure databases
SMRi | P76085 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4263013, 319 interactors |
DIPi | DIP-10430N |
STRINGi | 511145.b1398 |
Proteomic databases
PaxDbi | P76085 |
PRIDEi | P76085 |
Genome annotation databases
EnsemblBacteriai | AAC74480; AAC74480; b1398 BAE76428; BAE76428; BAE76428 |
GeneIDi | 945963 |
KEGGi | ecj:JW5218 eco:b1398 |
PATRICi | fig|1411691.4.peg.873 |
Organism-specific databases
EchoBASEi | EB3508 |
Phylogenomic databases
eggNOGi | COG1541, Bacteria |
HOGENOMi | CLU_035301_1_1_6 |
InParanoidi | P76085 |
PhylomeDBi | P76085 |
Enzyme and pathway databases
UniPathwayi | UPA00930 |
BioCyci | EcoCyc:G6719-MONOMER |
Miscellaneous databases
PROi | PR:P76085 |
Family and domain databases
CDDi | cd05913, PaaK, 1 hit |
Gene3Di | 3.30.300.30, 1 hit 3.40.50.12780, 1 hit |
InterProi | View protein in InterPro IPR045851, AMP-bd_C_sf IPR028154, AMP-dep_Lig_C IPR000873, AMP-dep_Synth/Lig IPR042099, ANL_N_sf IPR011880, PA_CoA_ligase |
Pfami | View protein in Pfam PF00501, AMP-binding, 1 hit PF14535, AMP-binding_C_2, 1 hit |
PIRSFi | PIRSF006444, PaaK, 1 hit |
TIGRFAMsi | TIGR02155, PA_CoA_ligase, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PAAK_ECOLI | |
Accessioni | P76085Primary (citable) accession number: P76085 Secondary accession number(s): O53018, Q2MBC8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 27, 2001 |
Last sequence update: | February 1, 1997 | |
Last modified: | February 23, 2022 | |
This is version 139 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families