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Protein

Acyl-coenzyme A thioesterase PaaI

Gene

paaI

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Thioesterase with a preference for ring-hydroxylated phenylacetyl-CoA esters. Hydrolyzes 3,4-dihydroxyphenylacetyl-CoA, 3-hydroxyphenylacetyl-CoA and 4-hydroxyphenylacetyl-CoA. Inactive towards 4-hydroxybenzoyl-CoA and 4-hydroxyphenacyl-CoA.2 Publications

Kineticsi

  1. KM=16 µM for 3,4-dihydroxyphenylacetyl-CoA
  2. KM=21 µM for 3-hydroxyphenylacetyl-CoA
  3. KM=35 µM for 4-hydroxyphenylacetyl-CoA

    pH dependencei

    Optimum pH is 7.5-9.

    Pathwayi: phenylacetate degradation

    This protein is involved in the pathway phenylacetate degradation, which is part of Aromatic compound metabolism.
    View all proteins of this organism that are known to be involved in the pathway phenylacetate degradation and in Aromatic compound metabolism.

    GO - Molecular functioni

    • CoA hydrolase activity Source: EcoCyc

    GO - Biological processi

    Keywordsi

    Molecular functionHydrolase

    Enzyme and pathway databases

    BioCyciEcoCyc:G6717-MONOMER
    MetaCyc:G6717-MONOMER
    BRENDAi3.1.2.B5 2026
    UniPathwayiUPA00930

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Acyl-coenzyme A thioesterase PaaI (EC:3.1.2.-)
    Alternative name(s):
    Phenylacetic acid degradation protein PaaI
    Gene namesi
    Name:paaI
    Synonyms:ydbV
    Ordered Locus Names:b1396, JW1391
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG13743 paaI

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi46N → A: Reduces activity 1000-fold. 1 Publication1
    Mutagenesisi52H → A: Reduces activity 100-fold. 1 Publication1
    Mutagenesisi61D → A: Loss of activity. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Initiator methionineiRemoved1 Publication
    ChainiPRO_00001566752 – 140Acyl-coenzyme A thioesterase PaaIAdd BLAST139

    Proteomic databases

    PaxDbiP76084
    PRIDEiP76084

    Expressioni

    Inductioni

    Activated by cAMP receptor protein (CRP), integration host factor (IHF) and by phenylacetyl-coenzyme A (PA-CoA) that prevents PaaX from binding its target sequences. Inhibited by PaaX.2 Publications

    Interactioni

    Subunit structurei

    Homotetramer.1 Publication

    Protein-protein interaction databases

    BioGridi4261735, 619 interactors
    DIPiDIP-10428N
    IntActiP76084, 2 interactors
    STRINGi316385.ECDH10B_1521

    Structurei

    Secondary structure

    1140
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Helixi2 – 22Combined sources21
    Beta strandi25 – 30Combined sources6
    Beta strandi33 – 39Combined sources7
    Helixi42 – 44Combined sources3
    Beta strandi49 – 51Combined sources3
    Helixi53 – 69Combined sources17
    Turni70 – 72Combined sources3
    Beta strandi76 – 84Combined sources9
    Beta strandi93 – 103Combined sources11
    Beta strandi105 – 115Combined sources11
    Beta strandi121 – 130Combined sources10
    Beta strandi133 – 135Combined sources3

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1PSUX-ray2.20A/B1-140[»]
    2FS2X-ray2.00A/B2-140[»]
    ProteinModelPortaliP76084
    SMRiP76084
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP76084

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the thioesterase PaaI family.Curated

    Phylogenomic databases

    eggNOGiENOG4108ZJI Bacteria
    COG2050 LUCA
    HOGENOMiHOG000066992
    InParanoidiP76084
    KOiK02614
    OMAiAFACNSH
    PhylomeDBiP76084

    Family and domain databases

    InterProiView protein in InterPro
    IPR029069 HotDog_dom_sf
    IPR011973 PaaD
    IPR003736 PAAI_dom
    IPR006683 Thioestr_dom
    PfamiView protein in Pfam
    PF03061 4HBT, 1 hit
    SUPFAMiSSF54637 SSF54637, 1 hit
    TIGRFAMsiTIGR02286 PaaD, 1 hit
    TIGR00369 unchar_dom_1, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P76084-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSHKAWQNAH AMYENDACAK ALGIDIISMD EGFAVVTMTV TAQMLNGHQS
    60 70 80 90 100
    CHGGQLFSLA DTAFAYACNS QGLAAVASAC TIDFLRPGFA GDTLTATAQV
    110 120 130 140
    RHQGKQTGVY DIEIVNQQQK TVALFRGKSH RIGGTITGEA
    Length:140
    Mass (Da):14,851
    Last modified:February 1, 1997 - v1
    Checksum:iA91DE9D2C8E8CF42
    GO

    Mass spectrometryi

    Molecular mass is 14720 Da from positions 2 - 140. Determined by ESI. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X97452 Genomic DNA Translation: CAA66098.1
    U00096 Genomic DNA Translation: AAC74478.1
    AP009048 Genomic DNA Translation: BAE76427.1
    PIRiG64890
    RefSeqiNP_415914.1, NC_000913.3
    WP_000018413.1, NZ_LN832404.1

    Genome annotation databases

    EnsemblBacteriaiAAC74478; AAC74478; b1396
    BAE76427; BAE76427; BAE76427
    GeneIDi945265
    KEGGiecj:JW1391
    eco:b1396
    PATRICifig|1411691.4.peg.875

    Similar proteinsi

    Entry informationi

    Entry nameiPAAI_ECOLI
    AccessioniPrimary (citable) accession number: P76084
    Secondary accession number(s): Q2MBC9
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: February 1, 1997
    Last modified: March 28, 2018
    This is version 130 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families
    5. Uncharacterized protein families (UPF)
      List of uncharacterized protein family (UPF) entries

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