UniProtKB - P76083 (PAAH_ECOLI)
Protein
3-hydroxyadipyl-CoA dehydrogenase
Gene
paaH
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the oxidation of 3-hydroxyadipyl-CoA to yield 3-oxoadipyl-CoA.2 Publications
Catalytic activityi
: phenylacetate degradation Pathwayi
This protein is involved in the pathway phenylacetate degradation, which is part of Aromatic compound metabolism.1 PublicationView all proteins of this organism that are known to be involved in the pathway phenylacetate degradation and in Aromatic compound metabolism.
GO - Molecular functioni
- 3-hydroxyacyl-CoA dehydrogenase activity Source: EcoCyc
- 3-hydroxybutyryl-CoA dehydrogenase activity Source: UniProtKB
- NAD+ binding Source: InterPro
GO - Biological processi
- fatty acid metabolic process Source: InterPro
- phenylacetate catabolic process Source: UniProtKB
Keywordsi
Molecular function | Oxidoreductase |
Ligand | NAD |
Enzyme and pathway databases
BioCyci | EcoCyc:G6716-MONOMER MetaCyc:G6716-MONOMER |
UniPathwayi | UPA00930 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:paaH Synonyms:ydbU Ordered Locus Names:b1395, JW1390 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Mutants accumulate 3-hydroxyadipate and are unable to use phenylacetate as a carbon source.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000109332 | 1 – 475 | 3-hydroxyadipyl-CoA dehydrogenaseAdd BLAST | 475 |
Proteomic databases
PaxDbi | P76083 |
PRIDEi | P76083 |
Expressioni
Inductioni
Activated by cAMP receptor protein (CRP), integration host factor (IHF) and by phenylacetyl-coenzyme A (PA-CoA) that prevents PaaX from binding its target sequences. Inhibited by PaaX.2 Publications
Interactioni
Subunit structurei
Homotrimer.
CuratedProtein-protein interaction databases
BioGRIDi | 4259599, 406 interactors |
IntActi | P76083, 1 interactor |
STRINGi | 511145.b1395 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P76083 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P76083 |
Family & Domainsi
Sequence similaritiesi
Belongs to the 3-hydroxyacyl-CoA dehydrogenase family.Curated
Phylogenomic databases
eggNOGi | COG1250, Bacteria |
HOGENOMi | CLU_009834_2_2_6 |
InParanoidi | P76083 |
PhylomeDBi | P76083 |
Family and domain databases
InterProi | View protein in InterPro IPR006180, 3-OHacyl-CoA_DH_CS IPR006176, 3-OHacyl-CoA_DH_NAD-bd IPR011967, 3-OHacyl-CoA_DH_PaaC IPR006108, 3HC_DH_C IPR041040, 3HCDH_RFF IPR008927, 6-PGluconate_DH-like_C_sf IPR036291, NAD(P)-bd_dom_sf |
Pfami | View protein in Pfam PF00725, 3HCDH, 2 hits PF02737, 3HCDH_N, 1 hit PF18321, 3HCDH_RFF, 1 hit |
SUPFAMi | SSF48179, SSF48179, 2 hits SSF51735, SSF51735, 1 hit |
TIGRFAMsi | TIGR02279, PaaC-3OHAcCoADH, 1 hit |
PROSITEi | View protein in PROSITE PS00067, 3HCDH, 1 hit |
i Sequence
Sequence statusi: Complete.
P76083-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MMINVQTVAV IGSGTMGAGI AEVAASHGHQ VLLYDISAEA LTRAIDGIHA
60 70 80 90 100
RLNSRVTRGK LTAETCERTL KRLIPVTDIH ALAAADLVIE AASERLEVKK
110 120 130 140 150
ALFAQLAEVC PPQTLLTTNT SSISITAIAA EIKNPERVAG LHFFNPAPVM
160 170 180 190 200
KLVEVVSGLA TAAEVVEQLC ELTLSWGKQP VRCHSTPGFI VNRVARPYYS
210 220 230 240 250
EAWRALEEQV AAPEVIDAAL RDGAGFPMGP LELTDLIGQD VNFAVTCSVF
260 270 280 290 300
NAFWQERRFL PSLVQQELVI GGRLGKKSGL GVYDWRAERE AVVGLEAVSD
310 320 330 340 350
SFSPMKVEKK SDGVTEIDDV LLIETQGETA QALAIRLARP VVVIDKMAGK
360 370 380 390 400
VVTIAAAAVN PDSATRKAIY YLQQQGKTVL QIADYPGMLI WRTVAMIINE
410 420 430 440 450
ALDALQKGVA SEQDIDTAMR LGVNYPYGPL AWGAQLGWQR ILRLLENLQH
460 470
HYGEERYRPC SLLRQRALLE SGYES
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 132 | I → V in strain: W. | 1 | |
Natural varianti | 344 | I → V in strain: W. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X97452 Genomic DNA Translation: CAA66097.1 U00096 Genomic DNA Translation: AAC74477.1 AP009048 Genomic DNA Translation: BAA15001.2 |
PIRi | F64890 |
RefSeqi | NP_415913.1, NC_000913.3 WP_000973360.1, NZ_SSZK01000012.1 |
Genome annotation databases
EnsemblBacteriai | AAC74477; AAC74477; b1395 BAA15001; BAA15001; BAA15001 |
GeneIDi | 945940 |
KEGGi | ecj:JW1390 eco:b1395 |
PATRICi | fig|511145.12.peg.1458 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X97452 Genomic DNA Translation: CAA66097.1 U00096 Genomic DNA Translation: AAC74477.1 AP009048 Genomic DNA Translation: BAA15001.2 |
PIRi | F64890 |
RefSeqi | NP_415913.1, NC_000913.3 WP_000973360.1, NZ_SSZK01000012.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3MOG | X-ray | 2.20 | A/B/C | 4-475 | [»] | |
SMRi | P76083 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259599, 406 interactors |
IntActi | P76083, 1 interactor |
STRINGi | 511145.b1395 |
Proteomic databases
PaxDbi | P76083 |
PRIDEi | P76083 |
Genome annotation databases
EnsemblBacteriai | AAC74477; AAC74477; b1395 BAA15001; BAA15001; BAA15001 |
GeneIDi | 945940 |
KEGGi | ecj:JW1390 eco:b1395 |
PATRICi | fig|511145.12.peg.1458 |
Organism-specific databases
EchoBASEi | EB3505 |
Phylogenomic databases
eggNOGi | COG1250, Bacteria |
HOGENOMi | CLU_009834_2_2_6 |
InParanoidi | P76083 |
PhylomeDBi | P76083 |
Enzyme and pathway databases
UniPathwayi | UPA00930 |
BioCyci | EcoCyc:G6716-MONOMER MetaCyc:G6716-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P76083 |
PROi | PR:P76083 |
Family and domain databases
InterProi | View protein in InterPro IPR006180, 3-OHacyl-CoA_DH_CS IPR006176, 3-OHacyl-CoA_DH_NAD-bd IPR011967, 3-OHacyl-CoA_DH_PaaC IPR006108, 3HC_DH_C IPR041040, 3HCDH_RFF IPR008927, 6-PGluconate_DH-like_C_sf IPR036291, NAD(P)-bd_dom_sf |
Pfami | View protein in Pfam PF00725, 3HCDH, 2 hits PF02737, 3HCDH_N, 1 hit PF18321, 3HCDH_RFF, 1 hit |
SUPFAMi | SSF48179, SSF48179, 2 hits SSF51735, SSF51735, 1 hit |
TIGRFAMsi | TIGR02279, PaaC-3OHAcCoADH, 1 hit |
PROSITEi | View protein in PROSITE PS00067, 3HCDH, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PAAH_ECOLI | |
Accessioni | P76083Primary (citable) accession number: P76083 Secondary accession number(s): O53016, P78289 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | February 1, 1997 | |
Last modified: | April 7, 2021 | |
This is version 155 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families