UniProtKB - P76082 (PAAF_ECOLI)
Protein
2,3-dehydroadipyl-CoA hydratase
Gene
paaF
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the reversible conversion of enzymatically produced 2,3-dehydroadipyl-CoA into 3-hydroxyadipyl-CoA.2 Publications
Catalytic activityi
: phenylacetate degradation Pathwayi
This protein is involved in the pathway phenylacetate degradation, which is part of Aromatic compound metabolism.View all proteins of this organism that are known to be involved in the pathway phenylacetate degradation and in Aromatic compound metabolism.
GO - Molecular functioni
- enoyl-CoA hydratase activity Source: UniProtKB
- identical protein binding Source: EcoCyc
GO - Biological processi
- fatty acid beta-oxidation Source: GO_Central
- phenylacetate catabolic process Source: EcoCyc
Keywordsi
Molecular function | Lyase |
Biological process | Fatty acid metabolism, Lipid metabolism |
Enzyme and pathway databases
BioCyci | EcoCyc:G6714-MONOMER |
BRENDAi | 4.2.1.17, 2026 |
UniPathwayi | UPA00930 |
Names & Taxonomyi
Protein namesi | Recommended name: 2,3-dehydroadipyl-CoA hydratase (EC:4.2.1.17)Alternative name(s): Enoyl-CoA hydratase |
Gene namesi | Name:paaF Synonyms:ydbR Ordered Locus Names:b1393, JW1388 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Mutants accumulate delta3-dehydroadipate and are unable to use phenylacetate as a carbon source.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000109330 | 1 – 255 | 2,3-dehydroadipyl-CoA hydrataseAdd BLAST | 255 |
Proteomic databases
PaxDbi | P76082 |
PRIDEi | P76082 |
Expressioni
Inductioni
Activated by cAMP receptor protein (CRP), integration host factor (IHF) and by phenylacetyl-coenzyme A (PA-CoA) that prevents PaaX from binding its target sequences. Inhibited by PaaX.2 Publications
Interactioni
Binary interactionsi
P76082
With | #Exp. | IntAct |
---|---|---|
paaG [P77467] | 3 | EBI-1115747,EBI-1118484 |
GO - Molecular functioni
- identical protein binding Source: EcoCyc
Protein-protein interaction databases
BioGRIDi | 4260970, 248 interactors |
ComplexPortali | CPX-5681, Enoyl CoA hydratase/isomerase complex |
DIPi | DIP-10425N |
IntActi | P76082, 2 interactors |
STRINGi | 511145.b1393 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P76082 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the enoyl-CoA hydratase/isomerase family.Curated
Phylogenomic databases
eggNOGi | COG1024, Bacteria |
HOGENOMi | CLU_009834_7_6_6 |
InParanoidi | P76082 |
PhylomeDBi | P76082 |
Family and domain databases
Gene3Di | 1.10.12.10, 1 hit |
InterProi | View protein in InterPro IPR029045, ClpP/crotonase-like_dom_sf IPR018376, Enoyl-CoA_hyd/isom_CS IPR001753, Enoyl-CoA_hydra/iso IPR014748, Enoyl-CoA_hydra_C |
Pfami | View protein in Pfam PF00378, ECH_1, 1 hit |
SUPFAMi | SSF52096, SSF52096, 1 hit |
PROSITEi | View protein in PROSITE PS00166, ENOYL_COA_HYDRATASE, 1 hit |
i Sequence
Sequence statusi: Complete.
P76082-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSELIVSRQQ RVLLLTLNRP AARNALNNAL LMQLVNELEA AATDTSISVC
60 70 80 90 100
VITGNARFFA AGADLNEMAE KDLAATLNDT RPQLWARLQA FNKPLIAAVN
110 120 130 140 150
GYALGAGCEL ALLCDVVVAG ENARFGLPEI TLGIMPGAGG TQRLIRSVGK
160 170 180 190 200
SLASKMVLSG ESITAQQAQQ AGLVSDVFPS DLTLEYALQL ASKMARHSPL
210 220 230 240 250
ALQAAKQALR QSQEVALQAG LAQERQLFTL LAATEDRHEG ISAFLQKRTP
DFKGR
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 11 | R → Q in strain: W. | 1 | |
Natural varianti | 32 | M → T in strain: W. | 1 | |
Natural varianti | 45 | T → S in strain: W. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X97452 Genomic DNA Translation: CAA66095.1 U00096 Genomic DNA Translation: AAC74475.1 AP009048 Genomic DNA Translation: BAA14999.2 |
PIRi | D64890 |
RefSeqi | NP_415911.1, NC_000913.3 WP_001292353.1, NZ_STEB01000005.1 |
Genome annotation databases
EnsemblBacteriai | AAC74475; AAC74475; b1393 BAA14999; BAA14999; BAA14999 |
GeneIDi | 946011 |
KEGGi | ecj:JW1388 eco:b1393 |
PATRICi | fig|1411691.4.peg.878 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X97452 Genomic DNA Translation: CAA66095.1 U00096 Genomic DNA Translation: AAC74475.1 AP009048 Genomic DNA Translation: BAA14999.2 |
PIRi | D64890 |
RefSeqi | NP_415911.1, NC_000913.3 WP_001292353.1, NZ_STEB01000005.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4FZW | X-ray | 2.55 | A/B | 1-255 | [»] | |
SMRi | P76082 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260970, 248 interactors |
ComplexPortali | CPX-5681, Enoyl CoA hydratase/isomerase complex |
DIPi | DIP-10425N |
IntActi | P76082, 2 interactors |
STRINGi | 511145.b1393 |
Proteomic databases
PaxDbi | P76082 |
PRIDEi | P76082 |
Genome annotation databases
EnsemblBacteriai | AAC74475; AAC74475; b1393 BAA14999; BAA14999; BAA14999 |
GeneIDi | 946011 |
KEGGi | ecj:JW1388 eco:b1393 |
PATRICi | fig|1411691.4.peg.878 |
Organism-specific databases
EchoBASEi | EB3503 |
Phylogenomic databases
eggNOGi | COG1024, Bacteria |
HOGENOMi | CLU_009834_7_6_6 |
InParanoidi | P76082 |
PhylomeDBi | P76082 |
Enzyme and pathway databases
UniPathwayi | UPA00930 |
BioCyci | EcoCyc:G6714-MONOMER |
BRENDAi | 4.2.1.17, 2026 |
Miscellaneous databases
PROi | PR:P76082 |
Family and domain databases
Gene3Di | 1.10.12.10, 1 hit |
InterProi | View protein in InterPro IPR029045, ClpP/crotonase-like_dom_sf IPR018376, Enoyl-CoA_hyd/isom_CS IPR001753, Enoyl-CoA_hydra/iso IPR014748, Enoyl-CoA_hydra_C |
Pfami | View protein in Pfam PF00378, ECH_1, 1 hit |
SUPFAMi | SSF52096, SSF52096, 1 hit |
PROSITEi | View protein in PROSITE PS00166, ENOYL_COA_HYDRATASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PAAF_ECOLI | |
Accessioni | P76082Primary (citable) accession number: P76082 Secondary accession number(s): O53014, P78288 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | February 1, 1997 | |
Last modified: | April 7, 2021 | |
This is version 145 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families